Restriction map for
pFN21AE1596
(Circular) (Six-base) MAPSORT of: pFN21AE1596.seq Check: 170 from: 1 to: 5767
pFN21AE1596 5767 bp
With 182 enzymes: SgfI *
January 5, 2010 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2652 2652
Size: 5767
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2712 3110 1937
Size: 775 398 4594
Fragments arranged by size:
4594 775 398
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2812 3830 5214 5482 1632
Size: 1180 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1180 1018 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2484 4666 5643 1203
Size: 1281 2182 977 1327
Fragments arranged by size:
2182 1327 1281 977
AgeI A'CCGG_T
Cuts at: 2955 4598 2955
Size: 1643 4124
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 2482
Size: 32 5735
AlwNI CAG_nnn'CTG
Cuts at: 3510 5082 3510
Size: 1572 4195
ApaI G_GGCC'C
Cuts at: 2035 2035
Size: 5767
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2842 3092 1870
Size: 73 83 816 250 4545
Fragments arranged by size:
4545 816 250 83 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2630 3088 4543 618
Size: 325 217 627 420 423 458 1455 1842
Fragments arranged by size:
1842 1455 627 458 423 420 325 217
BanII G_rGCy'C
Cuts at: 1391 2035 3086 1391
Size: 644 1051 4072
Fragments arranged by size:
4072 1051 644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BbvCI CC'TCA_GC
Cuts at: 2672 2672
Size: 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BfrBI ATG'CAT
Cuts at: 2789 2789
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3059 4378 136
Size: 107 122 71 631 605 1387 1319 1525
Fragments arranged by size:
1525 1387 1319 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2800 5762 2800
Size: 2962 2805
BlpI GC'TnA_GC
Cuts at: 2904 3184 2904
Size: 280 5487
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2635 3828 4984 1790
Size: 245 600 1193 1156 2573
Fragments arranged by size:
2573 1193 1156 600 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2943 3498 4456
Size: 416 53 83 399 24 1526 555 958
Cuts at: 4456 442
Size: 1753
Fragments arranged by size:
1753 1526 958 555 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2681 2879 4427 1239
Size: 1442 198 1548 2579
Fragments arranged by size:
2579 1548 1442 198
Bpu10I CC'TnA_GC
Cuts at: 2672 2717 2672
Size: 45 5722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2462 4430 882
Size: 617 963 1968 2219
Fragments arranged by size:
2219 1968 963 617
BsaAI yAC'GTr
Cuts at: 493 1204 2487 3558 493
Size: 711 1283 1071 2702
Fragments arranged by size:
2702 1283 1071 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3043 3574 3956 4591
Size: 53 83 186 936 1510 531 382 635
Cuts at: 4591 275
Size: 1451
Fragments arranged by size:
1510 1451 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2955 3036 4200 4598 4872 5019 5430 2955
Size: 81 1164 398 274 147 411 3292
Fragments arranged by size:
3292 1164 411 398 274 147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2867 2897 1178
Size: 30 165 30 1464 30 4048
Fragments arranged by size:
4048 1464 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2580 2993 1186
Size: 1394 413 3960
Fragments arranged by size:
3960 1394 413
BseYI C'CCAG_C
Cuts at: 2038 2502 4970 5466 2038
Size: 464 2468 496 2339
Fragments arranged by size:
2468 2339 496 464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5194
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2141 3828 3913 4984 2141
Size: 1687 85 1071 2924
Fragments arranged by size:
2924 1687 1071 85
BsmI GAATG_Cn'
Cuts at: 2791 3278 3371 2791
Size: 487 93 5187
Fragments arranged by size:
5187 487 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3034 3034
Size: 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2141 2635 3086 3828 3913 4984
Size: 399 245 106 494 451 742 85 1071
Cuts at: 4984 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 494 451 399 245 106
85
BspEI T'CCGG_A
Cuts at: 3036 5430 3036
Size: 2394 3373
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2652 2886 3122 844
Size: 1808 234 236 3489
Fragments arranged by size:
3489 1808 236 234
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2955 4411 4598 1700
Size: 110 1145 1456 187 2869
Fragments arranged by size:
2869 1456 1145 187 110
BsrGI T'GTAC_A
Cuts at: 96 2547 96
Size: 2451 3316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2800 3097 3464 3849 3866 5318 5404 5416
Size: 828 297 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 828 385 367 346 297 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2888 2888
Size: 5767
BtgI C'CryG_G
Cuts at: 513 1065 2124 2218 2260 2361 513
Size: 552 1059 94 42 101 3919
Fragments arranged by size:
3919 1059 552 101 94 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 2385 3283 4077 4097 2295
Size: 90 898 794 20 3965
Fragments arranged by size:
3965 898 794 90 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3214 4103 5732
Size: 54 1425 50 275 18 1384 889 1629
Cuts at: 5732 8
Size: 43
Fragments arranged by size:
1629 1425 1384 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3697 1353
Size: 2344 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3040 4354 4728 4874 387
Size: 1064 138 1451 1314 374 146 1280
Fragments arranged by size:
1451 1314 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2681 2812 2879 3830 4427 5214 5482
Size: 393 1049 131 67 951 597 787 268
Cuts at: 5482 1239
Size: 1524
Fragments arranged by size:
1524 1049 951 787 597 393 268 131
67
EcoO109I rG'GnC_Cy
Cuts at: 2031 2032 2312 2692 3061 2031
Size: 1 280 380 369 4737
Fragments arranged by size:
4737 380 369 280 1
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 4914 1678
Size: 3236 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2253 2867 2899 4408
Size: 32 135 32 649 32 614 32 1509
Cuts at: 4408 4440 4482 4514 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1509 649 614 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2306 2396 4881 5065 1305
Size: 1001 90 2485 184 2007
Fragments arranged by size:
2485 2007 1001 184 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 2729 3725 4084 4243 518
Size: 648 1189 374 996 359 159 2042
Fragments arranged by size:
2042 1189 996 648 374 359 159
MspA1I CmG'CkG
Cuts at: 1606 2864 2908 3183 3860 5008 5253 1606
Size: 1258 44 275 677 1148 245 2120
Fragments arranged by size:
2120 1258 1148 677 275 245 44
NcoI C'CATG_G
Cuts at: 513 1065 2260 513
Size: 552 1195 4020
Fragments arranged by size:
4020 1195 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 2791 2791
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3125 4670 1132
Size: 97 1896 1545 2229
Fragments arranged by size:
2229 1896 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2056 2557 2815 1348
Size: 708 501 258 4300
Fragments arranged by size:
4300 708 501 258
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PmlI CAC'GTG
Cuts at: 2487 2487
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2312 2692 2312
Size: 380 5387
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 2031 2031
Size: 5767
PspXI vC'TCGA_Gb
Cuts at: 2842 2842
Size: 5767
PstI C_TGCA'G
Cuts at: 830 3119 830
Size: 2289 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2864 2908 2864
Size: 44 5723
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SanDI GG'GwC_CC
Cuts at: 2312 2312
Size: 5767
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 3115 4250 4931 5122 826
Size: 219 2070 1135 681 191 1471
Fragments arranged by size:
2070 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 3094 1872
Size: 1222 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2842 3871 4772 5034 820
Size: 197 926 899 1029 901 262 1553
Fragments arranged by size:
1553 1029 926 901 899 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 2345 152
Size: 2193 3574
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 2573 1517
Size: 1056 4711
StyI C'CwwG_G
Cuts at: 513 1065 2260 2463 2607 3206 513
Size: 552 1195 203 144 599 3074
Fragments arranged by size:
3074 1195 599 552 203 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 2436 3794 3811 3964 4149 4594
Size: 1255 557 299 1358 17 153 185 445
Cuts at: 4594 325
Size: 1498
Fragments arranged by size:
1498 1358 1255 557 445 299 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 2587 2736
Size: 274 80 33 51 1443 570 40 149
Cuts at: 2736 4013 5725 96
Size: 1277 1712 138
Fragments arranged by size:
1712 1443 1277 570 274 149 138 80
51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2263 2295 2317 2349 2353 2385 2850 2882 5727
Size: 32 22 32 4 32 465 32 2845
Cuts at: 5727 5759 2263
Size: 32 2271
Fragments arranged by size:
2845 2271 465 32 32 32 32 32
22 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 2842 1943
Size: 899 4868
XmaI C'CCGG_G
Cuts at: 1870 3092 1870
Size: 1222 4545
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI