Restriction map for
pFN21AE1594
(Circular) (Six-base) MAPSORT of: pFN21AE1594.seq Check: 7716 from: 1 to: 5605
pFN21AE1594 5605 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2785 3415 278
Size: 53 83 186 936 1249 630 2468
Fragments arranged by size:
2468 1249 936 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2779 2948 1937
Size: 842 169 4594
Fragments arranged by size:
4594 842 169
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3668 5052 5320 1632
Size: 2036 1384 268 1917
Fragments arranged by size:
2036 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2430 1184
Size: 1246 4359
AlwNI CAG_nnn'CTG
Cuts at: 2459 2476 2710 3348 4920 2459
Size: 17 234 638 1572 3144
Fragments arranged by size:
3144 1572 638 234 17
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2149 2497 2914 3140 3292 5401 1493
Size: 656 348 417 226 152 2109 1697
Fragments arranged by size:
2109 1697 656 417 348 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 1870
Size: 73 987 4545
Fragments arranged by size:
4545 987 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2509 2641 2924 1391
Size: 1118 132 283 4072
Fragments arranged by size:
4072 1118 283 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 928
Size: 971 364 4270
Fragments arranged by size:
4270 971 364
BbvCI CC'TCA_GC
Cuts at: 2741 2741
Size: 5605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4822 1790
Size: 1876 1156 2573
Fragments arranged by size:
2573 1876 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2831 4265 1239
Size: 1592 1434 2579
Fragments arranged by size:
2579 1592 1434
Bpu10I CC'TnA_GC
Cuts at: 2169 2741 2169
Size: 572 5033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4268 882
Size: 617 2769 2219
Fragments arranged by size:
2769 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2782 3412 3794 4429
Size: 53 83 186 936 1249 630 382 635
Cuts at: 4429 275
Size: 1451
Fragments arranged by size:
1451 1249 936 635 630 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2499 1186
Size: 1313 4292
BseYI C'CCAG_C
Cuts at: 2076 2703 4808 5304 2076
Size: 627 2105 496 2377
Fragments arranged by size:
2377 2105 627 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 1163
Size: 573 414 4618
Fragments arranged by size:
4618 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2509 2641 3666 3751 4822 2509
Size: 132 1025 85 1071 3292
Fragments arranged by size:
3292 1071 1025 132 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2509 2641 2924 3666 3751 4822 1391
Size: 399 719 132 283 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 399 283 132 85
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 4100 4274 65
Size: 1183 1164 1688 174 1396
Fragments arranged by size:
1688 1396 1183 1164 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 2530 96
Size: 2434 3171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2183 794
Size: 1389 4216
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstXI CCAn_nnnn'nTGG
Cuts at: 2084 2084
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2456 2631 2935 3302 3687 3704 5156 5242
Size: 484 175 304 367 385 17 1452 86
Cuts at: 5242 5254 5600 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 484 385 367 346 304 175
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2058 2367 513
Size: 552 993 309 3751
Fragments arranged by size:
3751 993 552 309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2664 487
Size: 683 56 1438 3428
Fragments arranged by size:
3428 1438 683 56
BtsI GCAGTG_nn'
Cuts at: 2832 3121 3915 3935 2832
Size: 289 794 20 4502
Fragments arranged by size:
4502 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2519 2519
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 3052 3941
Size: 54 1425 50 275 18 313 909 889
Cuts at: 3941 5570 8
Size: 1629 43
Fragments arranged by size:
1629 1425 909 889 313 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 3535 1353
Size: 966 1216 3423
Fragments arranged by size:
3423 1216 966
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2507 2639 2507
Size: 132 5473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2831 3668 4265 5052 5320 1239
Size: 393 1199 837 597 787 268 1524
Fragments arranged by size:
1524 1199 837 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2266 2514 2266
Size: 248 5357
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2280 2280
Size: 5605
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2623 2655 4246
Size: 32 135 32 530 32 489 32 1591
Cuts at: 4246 4278 4320 4352 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1591 530 489 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2780 2949 3195 669
Size: 988 281 842 169 246 3079
Fragments arranged by size:
3079 988 842 281 246 169
HindIII A'AGCT_T
Cuts at: 748 2849 748
Size: 2101 3504
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 2181 2526 3204 2181
Size: 345 678 4582
Fragments arranged by size:
4582 678 345
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 10
Size: 54 1425 343 313 3470
Fragments arranged by size:
3470 1425 343 313 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3922 4081 518
Size: 648 2397 359 159 2042
Fragments arranged by size:
2397 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2552 2710 3021 3698 4846 5091 1606
Size: 946 158 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 946 677 311 245 158
NcoI C'CATG_G
Cuts at: 513 1065 2058 2367 513
Size: 552 993 309 3751
Fragments arranged by size:
3751 993 552 309
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2620 2963 4508 1132
Size: 97 1391 343 1545 2229
Fragments arranged by size:
2229 1545 1391 343 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2710 1108
Size: 1602 4003
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2206 2481 2957 830
Size: 1376 275 476 3478
Fragments arranged by size:
3478 1376 476 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2552 2710 2552
Size: 158 5447
SacI G_AGCT'C
Cuts at: 2509 2641 2509
Size: 132 5473
SalI G'TCGA_C
Cuts at: 1936 2778 2947 1936
Size: 842 169 4594
Fragments arranged by size:
4594 842 169
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SexAI A'CCwGG_T
Cuts at: 2515 2515
Size: 5605
SfcI C'TryA_G
Cuts at: 826 1045 2202 2418 2477 2953 4088 4769 4960
Size: 219 1157 216 59 476 1135 681 191
Cuts at: 4960 826
Size: 1471
Fragments arranged by size:
1471 1157 1135 681 476 219 216 191
59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2058 2367 2425 3044 513
Size: 552 993 309 58 619 3074
Fragments arranged by size:
3074 993 619 552 309 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3632 3649 3802 3987 4432 325
Size: 1255 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2530 3851 5563
Size: 274 80 33 51 1443 553 1321 1712
Cuts at: 5563 96
Size: 138
Fragments arranged by size:
1712 1443 1321 553 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2783 3413 276
Size: 53 83 186 936 1249 630 2468
Fragments arranged by size:
2468 1249 936 630 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI