Restriction map for
pFN21AE1589
(Circular) (Six-base) MAPSORT of: pFN21AE1589.seq Check: 4889 from: 1 to: 7861
pFN21AE1589 7861 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2280 2957 2280
Size: 677 7184
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5671 278
Size: 53 83 186 936 4135 2468
Fragments arranged by size:
4135 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2754 5204 1937
Size: 817 2450 4594
Fragments arranged by size:
4594 2450 817
Acc65I G'GTAC_C
Cuts at: 5182 5182
Size: 7861
AclI AA'CG_TT
Cuts at: 1563 2086 5988 6361 1563
Size: 523 3902 373 3063
Fragments arranged by size:
3902 3063 523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 4541 4700 5924 7308 7576 1632
Size: 807 2102 159 1224 1384 268 1917
Fragments arranged by size:
2102 1917 1384 1224 807 268 159
AfeI AGC'GCT
Cuts at: 4475 4475
Size: 7861
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7664
AflIII A'CryG_T
Cuts at: 1203 3368 5158 6760 7737 1203
Size: 2165 1790 1602 977 1327
Fragments arranged by size:
2165 1790 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4758 6692 4758
Size: 1934 5927
AhdI GACnn_n'nnGTC
Cuts at: 6590 6590
Size: 7861
AleI CACnn'nnGTG
Cuts at: 1166 2897 1166
Size: 1731 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2199 1184
Size: 1015 6846
AlwNI CAG_nnn'CTG
Cuts at: 4610 5604 7176 4610
Size: 994 1572 5295
Fragments arranged by size:
5295 1572 994
ApaLI G'TGCA_C
Cuts at: 2970 3508 5918 7074 2970
Size: 538 2410 1156 3757
Fragments arranged by size:
3757 2410 1156 538
ApoI r'AATT_y
Cuts at: 1493 2047 2379 2437 2729 3404 5170 5396 5548
Size: 554 332 58 292 675 1766 226 152
Cuts at: 5548 7657 1493
Size: 2109 1697
Fragments arranged by size:
2109 1766 1697 675 554 332 292 226
152 58
AseI AT'TA_AT
Cuts at: 160 1032 6415 160
Size: 872 5383 1606
Fragments arranged by size:
5383 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7861
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 4524 5186 1870
Size: 73 1032 1549 662 4545
Fragments arranged by size:
4545 1549 1032 662 73
BamHI G'GATC_C
Cuts at: 5191 5558 5191
Size: 367 7494
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 5182 6637 618
Size: 325 217 627 1208 2187 1455 1842
Fragments arranged by size:
2187 1842 1455 1208 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4648 4995 5180 1391
Size: 3257 347 185 4072
Fragments arranged by size:
4072 3257 347 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2934 4099 4547 928
Size: 971 1035 1165 448 4242
Fragments arranged by size:
4242 1165 1035 971 448
BbvCI CC'TCA_GC
Cuts at: 3524 3805 4676 3524
Size: 281 871 6709
Fragments arranged by size:
6709 871 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6052 6086 1903
Size: 34 4115 34 3678
Fragments arranged by size:
4115 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5753 6963 5753
Size: 1210 6651
BclI T'GATC_A
Cuts at: 1548 2485 1548
Size: 937 6924
BfrBI ATG'CAT
Cuts at: 3856 3856
Size: 7861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6472 136
Size: 107 122 71 631 605 4800 1525
Fragments arranged by size:
4800 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7856 7856
Size: 7861
BlpI GC'TnA_GC
Cuts at: 4026 4031 4059 5278 4026
Size: 5 28 1219 6609
Fragments arranged by size:
6609 1219 28 5
Bme1580I G_kGCm'C
Cuts at: 1790 2974 3512 5922 7078 1790
Size: 1184 538 2410 1156 2573
Fragments arranged by size:
2573 2410 1184 1156 538
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7861
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2554 4161 5592
Size: 416 53 83 399 24 1137 1607 1431
Cuts at: 5592 6550 442
Size: 958 1753
Fragments arranged by size:
1753 1607 1431 1137 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7861
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4539 4539
Size: 7861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2302 2733 3759 4064 4662 5024 6521 1239
Size: 1063 431 1026 305 598 362 1497 2579
Fragments arranged by size:
2579 1497 1063 1026 598 431 362 305
Bpu10I CC'TnA_GC
Cuts at: 3524 3805 4676 3524
Size: 281 871 6709
Fragments arranged by size:
6709 871 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2880 5986 6851 7149 2880
Size: 3106 865 298 3592
Fragments arranged by size:
3592 3106 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4907 5146 6524 882
Size: 617 3408 239 1378 2219
Fragments arranged by size:
3408 2219 1378 617 239
BsaAI yAC'GTr
Cuts at: 493 1204 2302 5652 493
Size: 711 1098 3350 2702
Fragments arranged by size:
3350 2702 1098 711
BsaBI GATnn'nnATC
Cuts at: 3312 7855 3312
Size: 4543 3318
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 5668 6050 6685
Size: 53 83 186 936 780 3355 382 635
Cuts at: 6685 275
Size: 1451
Fragments arranged by size:
3355 1451 936 780 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4118 4758 6294 6692 6966 7113 7524 4118
Size: 640 1536 398 274 147 411 4455
Fragments arranged by size:
4455 1536 640 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7636
Fragments arranged by size:
7636 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2372 4862 1186
Size: 1111 75 2490 4185
Fragments arranged by size:
4185 2490 1111 75
BseYI C'CCAG_C
Cuts at: 3978 3992 4148 4196 5016 7064 7560 3978
Size: 14 156 48 820 2048 496 4279
Fragments arranged by size:
4279 2048 820 496 156 48 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3904 4763 5311 6072 7100 1540
Size: 275 186 1903 859 548 761 1028 2301
Fragments arranged by size:
2301 1903 1028 859 761 548 275 186
BsiHKAI G_wGCw'C
Cuts at: 2788 2974 3512 4648 4995 5922 6007 7078 2788
Size: 186 538 1136 347 927 85 1071 3571
Fragments arranged by size:
3571 1136 1071 927 538 347 186 85
BsmI GAATG_Cn'
Cuts at: 2777 2952 5372 5465 2777
Size: 175 2420 93 5173
Fragments arranged by size:
5173 2420 175 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2320 3175 2320
Size: 855 7006
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2788 2974 3512 4648 4995 5180 5922
Size: 399 998 186 538 1136 347 185 742
Cuts at: 5922 6007 7078 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1136 1071 998 742 538 399 347
186 185 85
BspEI T'CCGG_A
Cuts at: 4118 7524 4118
Size: 3406 4455
BspHI T'CATG_A
Cuts at: 5028 5750 7480 5028
Size: 722 1730 5409
Fragments arranged by size:
5409 1730 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 2957 4398 5216 844
Size: 1436 677 1441 818 3489
Fragments arranged by size:
3489 1441 1436 818 677
BsrBI CCG'CTC
Cuts at: 1394 1823 5748 1394
Size: 429 3925 3507
Fragments arranged by size:
3925 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2106 2566 3009 3642 4356 6356 6530
Size: 1183 858 460 443 633 714 2000 174
Cuts at: 6530 65
Size: 1396
Fragments arranged by size:
2000 1396 1183 858 714 633 460 443
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3048 4758 6505 6692 1700
Size: 110 1238 1710 1747 187 2869
Fragments arranged by size:
2869 1747 1710 1238 187 110
BsrGI T'GTAC_A
Cuts at: 96 3310 3631 96
Size: 3214 321 4326
Fragments arranged by size:
4326 3214 321
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7861
BssSI C'ACGA_G
Cuts at: 5921 6933 5921
Size: 1012 6849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3154 4411 794
Size: 2360 1257 4244
Fragments arranged by size:
4244 2360 1257
BstBI TT'CG_AA
Cuts at: 6665 6665
Size: 7861
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7861
BstXI CCAn_nnnn'nTGG
Cuts at: 2157 2361 4238 2157
Size: 204 1877 5780
Fragments arranged by size:
5780 1877 204
BstYI r'GATC_y
Cuts at: 1972 2739 3559 4606 5191 5558 5943 5960 7412
Size: 767 820 1047 585 367 385 17 1452
Cuts at: 7412 7498 7510 7856 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1047 820 767 585 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4338 4886 4338
Size: 548 7313
BtgI C'CryG_G
Cuts at: 513 1065 2004 4269 513
Size: 552 939 2265 4105
Fragments arranged by size:
4105 2265 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7122
Fragments arranged by size:
7122 683 56
BtsI GCAGTG_nn'
Cuts at: 5377 6171 6191 5377
Size: 794 20 7047
Fragments arranged by size:
7047 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7861
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2863 2898 529
Size: 35 2299 35 5492
Fragments arranged by size:
5492 2299 35 35
DraI TTT'AAA
Cuts at: 2613 5165 2613
Size: 2552 5309
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6868 809
Size: 507 5552 1802
Fragments arranged by size:
5552 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3132 4899 5308
Size: 54 1425 50 275 18 1302 1767 409
Cuts at: 5308 6197 7826 8
Size: 889 1629 43
Fragments arranged by size:
1767 1629 1425 1302 889 409 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5308 1537
Size: 275 3496 4090
Fragments arranged by size:
4090 3496 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2453 4044 4062 4425 5791 1353
Size: 1100 1591 18 363 1366 3423
Fragments arranged by size:
3423 1591 1366 1100 363 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 6448 6822 6968 387
Size: 1064 138 660 4199 374 146 1280
Fragments arranged by size:
4199 1280 1064 660 374 146 138
EcoICRI GAG'CTC
Cuts at: 4646 4993 4646
Size: 347 7514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2439 2733 3759 4064 4541 4662
Size: 393 670 137 294 1026 305 477 121
Cuts at: 4662 4700 5024 5924 6521 7308 7576 1239
Size: 38 324 900 597 787 268 1524
Fragments arranged by size:
1524 1026 900 787 670 597 477 393
324 305 294 268 137 121 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3238 3873 3238
Size: 635 7226
EcoRI G'AATT_C
Cuts at: 2379 5170 5548 2379
Size: 2791 378 4692
Fragments arranged by size:
4692 2791 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 7861
FspI TGC'GCA
Cuts at: 6367 6367
Size: 7861
HaeII r_GCGC'y
Cuts at: 1678 4477 7008 1678
Size: 2799 2531 2531
Fragments arranged by size:
2799 2531 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2247 2279 2470 2502 2729
Size: 32 135 32 675 32 191 32 227
Cuts at: 2729 2731 2761 2763 3512 3544 3551 3583 5059
Size: 2 30 2 749 32 7 32 1476
Cuts at: 5059 5091 6502 6534 6576 6608 1373
Size: 32 1411 32 42 32 2626
Fragments arranged by size:
2626 1476 1411 749 675 227 191 135
42 32 32 32 32 32 32 32
32 32 30 7 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5205 5451 669
Size: 988 281 3267 246 3079
Fragments arranged by size:
3267 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3738 4712 748
Size: 2990 974 3897
Fragments arranged by size:
3897 2990 974
HpaI GTT'AAC
Cuts at: 5451 5451
Size: 7861
KpnI G_GTAC'C
Cuts at: 5186 5186
Size: 7861
MfeI C'AATT_G
Cuts at: 5460 5460
Size: 7861
MluI A'CGCG_T
Cuts at: 7737 7737
Size: 7861
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2701 4468 6975 7159 1305
Size: 1396 1767 2507 184 2007
Fragments arranged by size:
2507 2007 1767 1396 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3134 4901 10
Size: 54 1425 343 1302 1767 2970
Fragments arranged by size:
2970 1767 1425 1302 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2897 3326 4079 4268 4814 5819
Size: 648 1015 716 429 753 189 546 1005
Cuts at: 5819 6178 6337 518
Size: 359 159 2042
Fragments arranged by size:
2042 1015 1005 753 716 648 546 429
359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2012 5277 5954 7102 7347 1606
Size: 406 3265 677 1148 245 2120
Fragments arranged by size:
3265 2120 1148 677 406 245
NaeI GCC'GGC
Cuts at: 3050 3050
Size: 7861
NcoI C'CATG_G
Cuts at: 513 1065 2004 4269 513
Size: 552 939 2265 4105
Fragments arranged by size:
4105 2265 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7861
NgoMIV G'CCGG_C
Cuts at: 3048 3048
Size: 7861
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7861
NotI GC'GGCC_GC
Cuts at: 5308 5308
Size: 7861
NsiI A_TGCA'T
Cuts at: 3858 3858
Size: 7861
NspI r_CATG'y
Cuts at: 1132 1229 2243 3157 3372 5219 6764 1132
Size: 97 1014 914 215 1847 1545 2229
Fragments arranged by size:
2229 1847 1545 1014 914 215 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7861
PciI A'CATG_T
Cuts at: 3368 6760 3368
Size: 3392 4469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4426 1108
Size: 3318 4543
PfoI T'CCnGG_A
Cuts at: 4041 4041
Size: 7861
PmeI GTTT'AAAC
Cuts at: 5165 5165
Size: 7861
PmlI CAC'GTG
Cuts at: 2302 2302
Size: 7861
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5930 5962 7443 7475 5930
Size: 32 1481 32 6316
Fragments arranged by size:
6316 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7861
PsiI TTA'TAA
Cuts at: 5431 5431
Size: 7861
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2071 2103 2071
Size: 32 7829
PstI C_TGCA'G
Cuts at: 830 4615 5213 830
Size: 3785 598 3478
Fragments arranged by size:
3785 3478 598
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7861
PvuII CAG'CTG
Cuts at: 2012 2012
Size: 7861
SacI G_AGCT'C
Cuts at: 4648 4995 4648
Size: 347 7514
SalI G'TCGA_C
Cuts at: 1936 5203 1936
Size: 3267 4594
SapI GCTCTTCn'nnn_
Cuts at: 2453 4062 4425 2453
Size: 1609 363 5889
Fragments arranged by size:
5889 1609 363
SbfI CC_TGCA'GG
Cuts at: 5213 5213
Size: 7861
ScaI AGT'ACT
Cuts at: 2056 3727 6109 7821 2056
Size: 1671 2382 1712 2096
Fragments arranged by size:
2382 2096 1712 1671
SexAI A'CCwGG_T
Cuts at: 4230 4230
Size: 7861
SfcI C'TryA_G
Cuts at: 826 1045 2755 3958 4377 4611 5209 6344 7025
Size: 219 1710 1203 419 234 598 1135 681
Cuts at: 7025 7216 826
Size: 191 1471
Fragments arranged by size:
1710 1471 1203 1135 681 598 419 234
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7861
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7861
SmaI CCC'GGG
Cuts at: 1872 5188 1872
Size: 3316 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2895 4524 5965 6866 7128 820
Size: 197 926 952 1629 1441 901 262 1553
Fragments arranged by size:
1629 1553 1441 952 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5652 493
Size: 5159 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7861
SphI G_CATG'C
Cuts at: 1132 3157 5219 1132
Size: 2025 2062 3774
Fragments arranged by size:
3774 2062 2025
SspI AAT'ATT
Cuts at: 5 52 5785 5
Size: 47 5733 2081
Fragments arranged by size:
5733 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7861
StyI C'CwwG_G
Cuts at: 513 1065 2004 4269 5300 513
Size: 552 939 2265 1031 3074
Fragments arranged by size:
3074 2265 1031 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2364 3890 4955 4976 5888 5905 6058
Size: 1255 784 1526 1065 21 912 17 153
Cuts at: 6058 6243 6688 325
Size: 185 445 1498
Fragments arranged by size:
1526 1498 1255 1065 912 784 445 185
153 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2054 3310 3380
Size: 274 80 33 51 1443 77 1256 70
Cuts at: 3380 3596 3631 3725 5049 6107 7819 96
Size: 216 35 94 1324 1058 1712 138
Fragments arranged by size:
1712 1443 1324 1256 1058 274 216 138
94 80 77 70 51 35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3466 3498 3814 3846 7821 7853 3466
Size: 32 316 32 3975 32 3474
Fragments arranged by size:
3975 3474 316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7861
XbaI T'CTAG_A
Cuts at: 2524 3613 5197 2524
Size: 1089 1584 5188
Fragments arranged by size:
5188 1584 1089
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2358 2358
Size: 7861
XhoI C'TCGA_G
Cuts at: 1943 4524 1943
Size: 2581 5280
XmaI C'CCGG_G
Cuts at: 1870 5186 1870
Size: 3316 4545
XmnI GAAnn'nnTTC
Cuts at: 2441 2721 4497 5990 2441
Size: 280 1776 1493 4312
Fragments arranged by size:
4312 1776 1493 280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5669 276
Size: 53 83 186 936 4135 2468
Fragments arranged by size:
4135 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I
DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI
PacI PpuMI PspOMI PspXI RsrII SacII SanDI SfiI
SfoI SrfI SwaI