(Circular) (Six-base) MAPSORT of: pFN21AE1581.seq Check: 73 from: 1 to: 4972 pFN21AE1581 4972 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2782 278 Size: 53 83 186 936 1246 2468 Fragments arranged by size: 2468 1246 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2315 1937 Size: 378 4594 Acc65I G'GTAC_C Cuts at: 2293 2293 Size: 4972 AclI AA'CG_TT Cuts at: 1563 3099 3472 1563 Size: 1536 373 3063 Fragments arranged by size: 3063 1536 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3035 4419 4687 1632 Size: 1403 1384 268 1917 Fragments arranged by size: 1917 1403 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4775 AflIII A'CryG_T Cuts at: 1203 2040 3871 4848 1203 Size: 837 1831 977 1327 Fragments arranged by size: 1831 1327 977 837 AgeI A'CCGG_T Cuts at: 3803 3803 Size: 4972 AhdI GACnn_n'nnGTC Cuts at: 3701 3701 Size: 4972 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4972 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4972 AlwNI CAG_nnn'CTG Cuts at: 2089 2715 4287 2089 Size: 626 1572 2774 Fragments arranged by size: 2774 1572 626 ApaLI G'TGCA_C Cuts at: 3029 4185 3029 Size: 1156 3816 ApoI r'AATT_y Cuts at: 1493 2138 2281 2507 2659 4768 1493 Size: 645 143 226 152 2109 1697 Fragments arranged by size: 2109 1697 645 226 152 143 AseI AT'TA_AT Cuts at: 160 1032 3526 160 Size: 872 2494 1606 Fragments arranged by size: 2494 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4972 AvaI C'yCGr_G Cuts at: 1870 1943 2297 1870 Size: 73 354 4545 Fragments arranged by size: 4545 354 73 BamHI G'GATC_C Cuts at: 2302 2669 2302 Size: 367 4605 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2293 3748 618 Size: 325 217 627 506 1455 1842 Fragments arranged by size: 1842 1455 627 506 325 217 BanII G_rGCy'C Cuts at: 1391 2291 1391 Size: 900 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4001 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3163 3197 1903 Size: 34 1226 34 3678 Fragments arranged by size: 3678 1226 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2864 4074 2864 Size: 1210 3762 BclI T'GATC_A Cuts at: 1548 2025 1548 Size: 477 4495 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3583 136 Size: 107 122 71 631 605 1911 1525 Fragments arranged by size: 1911 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4967 4967 Size: 4972 BlpI GC'TnA_GC Cuts at: 2389 2389 Size: 4972 Bme1580I G_kGCm'C Cuts at: 1790 3033 4189 1790 Size: 1243 1156 2573 Fragments arranged by size: 2573 1243 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4972 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2703 3661 442 Size: 416 53 83 399 24 1286 958 1753 Fragments arranged by size: 1753 1286 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4972 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 3632 1239 Size: 872 1521 2579 Fragments arranged by size: 2579 1521 872 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3097 3962 4260 3097 Size: 865 298 3809 Fragments arranged by size: 3809 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3635 882 Size: 617 2136 2219 Fragments arranged by size: 2219 2136 617 BsaAI yAC'GTr Cuts at: 493 1204 2763 493 Size: 711 1559 2702 Fragments arranged by size: 2702 1559 711 BsaBI GATnn'nnATC Cuts at: 4966 4966 Size: 4972 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2779 3161 3796 275 Size: 53 83 186 936 1246 382 635 1451 Fragments arranged by size: 1451 1246 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3405 3803 4077 4224 4635 3405 Size: 398 274 147 411 3742 Fragments arranged by size: 3742 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4747 Fragments arranged by size: 4747 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4972 BseYI C'CCAG_C Cuts at: 4175 4671 4175 Size: 496 4476 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2014 2422 3183 4211 1540 Size: 275 186 13 408 761 1028 2301 Fragments arranged by size: 2301 1028 761 408 275 186 13 BsiHKAI G_wGCw'C Cuts at: 3033 3118 4189 3033 Size: 85 1071 3816 Fragments arranged by size: 3816 1071 85 BsmI GAATG_Cn' Cuts at: 2483 2576 2483 Size: 93 4879 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2291 3033 3118 4189 1391 Size: 399 501 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 501 399 85 BspEI T'CCGG_A Cuts at: 4635 4635 Size: 4972 BspHI T'CATG_A Cuts at: 2861 4591 2861 Size: 1730 3242 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2327 844 Size: 1483 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2859 1394 Size: 429 1036 3507 Fragments arranged by size: 3507 1036 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2115 2167 3467 3641 65 Size: 1183 867 52 1300 174 1396 Fragments arranged by size: 1396 1300 1183 867 174 52 BsrFI r'CCGG_y Cuts at: 1700 1810 3616 3803 1700 Size: 110 1806 187 2869 Fragments arranged by size: 2869 1806 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4972 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4972 BssSI C'ACGA_G Cuts at: 3032 4044 3032 Size: 1012 3960 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4972 BstBI TT'CG_AA Cuts at: 3776 3776 Size: 4972 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4972 BstYI r'GATC_y Cuts at: 1972 2302 2669 3054 3071 4523 4609 4621 4967 Size: 330 367 385 17 1452 86 12 346 Cuts at: 4967 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 330 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4420 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4233 Fragments arranged by size: 4233 683 56 BtsI GCAGTG_nn' Cuts at: 2488 3282 3302 2488 Size: 794 20 4158 Fragments arranged by size: 4158 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4972 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4937 DraI TTT'AAA Cuts at: 2276 2276 Size: 4972 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3979 809 Size: 507 2663 1802 Fragments arranged by size: 2663 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 2419 3308 Size: 54 1425 50 275 18 417 172 889 Cuts at: 3308 4937 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 417 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2419 1537 Size: 275 607 4090 Fragments arranged by size: 4090 607 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 2902 1353 Size: 690 859 3423 Fragments arranged by size: 3423 859 690 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3559 3933 4079 387 Size: 1064 138 1970 374 146 1280 Fragments arranged by size: 1970 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 3035 3632 4419 4687 1239 Size: 393 479 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 479 393 268 EcoRI G'AATT_C Cuts at: 2281 2659 2281 Size: 378 4594 FspI TGC'GCA Cuts at: 3478 3478 Size: 4972 HaeII r_GCGC'y Cuts at: 1678 2090 4119 1678 Size: 412 2029 2531 Fragments arranged by size: 2531 2029 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3613 3645 3687 3719 1373 Size: 32 135 32 2041 32 42 32 2626 Fragments arranged by size: 2626 2041 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2316 2562 669 Size: 988 281 378 246 3079 Fragments arranged by size: 3079 988 378 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4972 HpaI GTT'AAC Cuts at: 2562 2562 Size: 4972 KpnI G_GTAC'C Cuts at: 2297 2297 Size: 4972 MfeI C'AATT_G Cuts at: 2571 2571 Size: 4972 MluI A'CGCG_T Cuts at: 4848 4848 Size: 4972 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 4086 4270 1305 Size: 720 2061 184 2007 Fragments arranged by size: 2061 2007 720 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 10 Size: 54 1425 343 417 2733 Fragments arranged by size: 2733 1425 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2930 3289 3448 518 Size: 648 1764 359 159 2042 Fragments arranged by size: 2042 1764 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2388 3065 4213 4458 1606 Size: 782 677 1148 245 2120 Fragments arranged by size: 2120 1148 782 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4420 NdeI CA'TA_TG Cuts at: 387 387 Size: 4972 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4972 NotI GC'GGCC_GC Cuts at: 2419 2419 Size: 4972 NspI r_CATG'y Cuts at: 1132 1229 2044 2330 3875 1132 Size: 97 815 286 1545 2229 Fragments arranged by size: 2229 1545 815 286 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4972 PciI A'CATG_T Cuts at: 2040 3871 2040 Size: 1831 3141 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4972 PmeI GTTT'AAAC Cuts at: 2276 2276 Size: 4972 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3041 3073 4554 4586 3041 Size: 32 1481 32 3427 Fragments arranged by size: 3427 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4972 PsiI TTA'TAA Cuts at: 2542 2542 Size: 4972 PstI C_TGCA'G Cuts at: 830 2324 830 Size: 1494 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4972 SalI G'TCGA_C Cuts at: 1936 2314 1936 Size: 378 4594 SbfI CC_TGCA'GG Cuts at: 2324 2324 Size: 4972 ScaI AGT'ACT Cuts at: 3220 4932 3220 Size: 1712 3260 SfcI C'TryA_G Cuts at: 826 1045 2320 3455 4136 4327 826 Size: 219 1275 1135 681 191 1471 Fragments arranged by size: 1471 1275 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4972 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4972 SmaI CCC'GGG Cuts at: 1872 2299 1872 Size: 427 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3076 3977 4239 820 Size: 197 926 1133 901 262 1553 Fragments arranged by size: 1553 1133 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2763 493 Size: 2270 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4972 SphI G_CATG'C Cuts at: 1132 2330 1132 Size: 1198 3774 SspI AAT'ATT Cuts at: 5 52 2896 5 Size: 47 2844 2081 Fragments arranged by size: 2844 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4972 StyI C'CwwG_G Cuts at: 513 1065 2411 513 Size: 552 1346 3074 Fragments arranged by size: 3074 1346 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2028 2999 3016 3169 3354 3799 325 Size: 1255 448 971 17 153 185 445 1498 Fragments arranged by size: 1498 1255 971 448 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 3218 4930 Size: 274 80 33 51 1443 174 1067 1712 Cuts at: 4930 96 Size: 138 Fragments arranged by size: 1712 1443 1067 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4932 4964 4932 Size: 32 4940 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4972 XbaI T'CTAG_A Cuts at: 2308 2308 Size: 4972 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4972 XmaI C'CCGG_G Cuts at: 1870 2297 1870 Size: 427 4545 XmnI GAAnn'nnTTC Cuts at: 3101 3101 Size: 4972 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2780 276 Size: 53 83 186 936 1246 2468 Fragments arranged by size: 2468 1246 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI