Restriction map for
pFN21AE1573
(Circular) (Six-base) MAPSORT of: pFN21AE1573.seq Check: 6421 from: 1 to: 7585
pFN21AE1573 7585 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4759 4759
Size: 7585
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5395 278
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2088 3330 4928 1937
Size: 151 1242 1598 4594
Fragments arranged by size:
4594 1598 1242 151
Acc65I G'GTAC_C
Cuts at: 4906 4906
Size: 7585
AclI AA'CG_TT
Cuts at: 1563 5712 6085 1563
Size: 4149 373 3063
Fragments arranged by size:
4149 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3276 3788 3986 4338 5648 7032 7300 1632
Size: 1644 512 198 352 1310 1384 268 1917
Fragments arranged by size:
1917 1644 1384 1310 512 352 268 198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7388
AflIII A'CryG_T
Cuts at: 1203 2781 3110 4513 6484 7461 1203
Size: 1578 329 1403 1971 977 1327
Fragments arranged by size:
1971 1578 1403 1327 977 329
AgeI A'CCGG_T
Cuts at: 6416 6416
Size: 7585
AhdI GACnn_n'nnGTC
Cuts at: 6314 6314
Size: 7585
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7585
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4656 4688 4656
Size: 32 7553
AlwNI CAG_nnn'CTG
Cuts at: 4538 4775 5328 6900 4538
Size: 237 553 1572 5223
Fragments arranged by size:
5223 1572 553 237
ApaLI G'TGCA_C
Cuts at: 5642 6798 5642
Size: 1156 6429
ApoI r'AATT_y
Cuts at: 1493 2612 3456 3587 4200 4894 5120 5272 7381
Size: 1119 844 131 613 694 226 152 2109
Cuts at: 7381 1493
Size: 1697
Fragments arranged by size:
2109 1697 1119 844 694 613 226 152
131
AseI AT'TA_AT
Cuts at: 160 1032 3084 6139 160
Size: 872 2052 3055 1606
Fragments arranged by size:
3055 2052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
AvaI C'yCGr_G
Cuts at: 1870 1943 3381 4014 4910 1870
Size: 73 1438 633 896 4545
Fragments arranged by size:
4545 1438 896 633 73
BamHI G'GATC_C
Cuts at: 4829 4915 5282 4829
Size: 86 367 7132
Fragments arranged by size:
7132 367 86
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2713 4906 6361 618
Size: 325 217 627 926 2193 1455 1842
Fragments arranged by size:
2193 1842 1455 926 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4904 1391
Size: 3513 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 928
Size: 971 416 6198
Fragments arranged by size:
6198 971 416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5776 5810 1903
Size: 34 3839 34 3678
Fragments arranged by size:
3839 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5477 6687 5477
Size: 1210 6375
BclI T'GATC_A
Cuts at: 1548 2119 2142 4399 1548
Size: 571 23 2257 4734
Fragments arranged by size:
4734 2257 571 23
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 6196 136
Size: 107 122 71 631 605 437 4087 1525
Fragments arranged by size:
4087 1525 631 605 437 122 107 71
BglII A'GATC_T
Cuts at: 3013 4046 4733 7580 3013
Size: 1033 687 2847 3018
Fragments arranged by size:
3018 2847 1033 687
BlpI GC'TnA_GC
Cuts at: 5002 5002
Size: 7585
Bme1580I G_kGCm'C
Cuts at: 1790 5646 6802 1790
Size: 3856 1156 2573
Fragments arranged by size:
3856 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3418 1318
Size: 2100 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2072 2117 5316
Size: 416 53 83 399 24 655 45 3199
Cuts at: 5316 6274 442
Size: 958 1753
Fragments arranged by size:
3199 1753 958 655 416 399 83 53
45 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7585
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4027 4029 4027
Size: 2 7583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2364 2866 3938 6245 1239
Size: 1125 502 1072 2307 2579
Fragments arranged by size:
2579 2307 1125 1072 502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3288 5710 6575 6873 3288
Size: 2422 865 298 4000
Fragments arranged by size:
4000 2422 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4000 6248 882
Size: 617 2501 2248 2219
Fragments arranged by size:
2501 2248 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3068 4209 5376 493
Size: 711 1864 1141 1167 2702
Fragments arranged by size:
2702 1864 1167 1141 711
BsaBI GATnn'nnATC
Cuts at: 7579 7579
Size: 7585
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5392 5774 6409 275
Size: 53 83 186 936 3859 382 635 1451
Fragments arranged by size:
3859 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3039 6018 6416 6690 6837 7248 3039
Size: 2979 398 274 147 411 3376
Fragments arranged by size:
3376 2979 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 2362 2392 2949
Size: 30 165 30 801 30 128 30 557
Cuts at: 2949 2979 1178
Size: 30 5784
Fragments arranged by size:
5784 801 557 165 128 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2840 3376 1186
Size: 1654 536 5395
Fragments arranged by size:
5395 1654 536
BseYI C'CCAG_C
Cuts at: 4833 4837 6788 7284 4833
Size: 4 1951 496 5134
Fragments arranged by size:
5134 1951 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4788 1163
Size: 573 3052 3960
Fragments arranged by size:
3960 3052 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5035 5796 6824 1540
Size: 275 186 3034 761 1028 2301
Fragments arranged by size:
3034 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5646 5731 6802 5646
Size: 85 1071 6429
Fragments arranged by size:
6429 1071 85
BsmI GAATG_Cn'
Cuts at: 5096 5189 5096
Size: 93 7492
BsmBI CGTCTCn'nnnn_
Cuts at: 2857 4427 2857
Size: 1570 6015
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4904 5646 5731 6802 1391
Size: 399 3114 742 85 1071 2174
Fragments arranged by size:
3114 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7248 7248
Size: 7585
BspHI T'CATG_A
Cuts at: 3282 4116 5474 7204 3282
Size: 834 1358 1730 3663
Fragments arranged by size:
3663 1730 1358 834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4371 4759 4940 844
Size: 3527 388 181 3489
Fragments arranged by size:
3527 3489 388 181
BsrBI CCG'CTC
Cuts at: 1394 1823 5472 1394
Size: 429 3649 3507
Fragments arranged by size:
3649 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4372 6080 6254 65
Size: 1183 3124 1708 174 1396
Fragments arranged by size:
3124 1708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6229 6416 1700
Size: 110 4419 187 2869
Fragments arranged by size:
4419 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2605 2798 96
Size: 2509 193 4883
Fragments arranged by size:
4883 2509 193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7585
BssSI C'ACGA_G
Cuts at: 5645 6657 5645
Size: 1012 6573
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7585
BstBI TT'CG_AA
Cuts at: 6389 6389
Size: 7585
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7585
BstXI CCAn_nnnn'nTGG
Cuts at: 3143 3916 4763 3143
Size: 773 847 5965
Fragments arranged by size:
5965 847 773
BstYI r'GATC_y
Cuts at: 1972 2578 3013 4046 4733 4829 4915 5282 5667
Size: 606 435 1033 687 96 86 367 385
Cuts at: 5667 5684 7136 7222 7234 7580 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1033 687 606 435 385 367
346 96 86 86 17 12
BstZ17I GTA'TAC
Cuts at: 2089 2089
Size: 7585
Bsu36I CC'TnA_GG
Cuts at: 2175 2175
Size: 7585
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7033
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6846
Fragments arranged by size:
6846 683 56
BtsI GCAGTG_nn'
Cuts at: 5101 5895 5915 5101
Size: 794 20 6771
Fragments arranged by size:
6771 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7550
DraI TTT'AAA
Cuts at: 2230 3571 4698 4889 2230
Size: 1341 1127 191 4926
Fragments arranged by size:
4926 1341 1127 191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6592 809
Size: 507 5276 1802
Fragments arranged by size:
5276 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4105 5032 5921
Size: 54 1425 50 275 18 2275 927 889
Cuts at: 5921 7550 8
Size: 1629 43
Fragments arranged by size:
2275 1629 1425 927 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5032 1537
Size: 275 3220 4090
Fragments arranged by size:
4090 3220 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 2703 4359 5515 1353
Size: 1269 81 1656 1156 3423
Fragments arranged by size:
3423 1656 1269 1156 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6172 6546 6692 387
Size: 1064 138 4583 374 146 1280
Fragments arranged by size:
4583 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2364 2866 3276 3788 3938 3986 4338
Size: 393 732 502 410 512 150 48 352
Cuts at: 4338 5648 6245 7032 7300 1239
Size: 1310 597 787 268 1524
Fragments arranged by size:
1524 1310 787 732 597 512 502 410
393 352 268 150 48
EcoO109I rG'GnC_Cy
Cuts at: 2055 4841 2055
Size: 2786 4799
EcoRI G'AATT_C
Cuts at: 2612 4894 5272 2612
Size: 2282 378 4925
Fragments arranged by size:
4925 2282 378
EcoRV GAT'ATC
Cuts at: 3466 4850 3466
Size: 1384 6201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 3220 4293 2284
Size: 936 1073 5576
Fragments arranged by size:
5576 1073 936
FspI TGC'GCA
Cuts at: 6091 6091
Size: 7585
HaeII r_GCGC'y
Cuts at: 1678 4144 6732 1678
Size: 2466 2588 2531
Fragments arranged by size:
2588 2531 2466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2362 2394 2949 2981 6226
Size: 32 135 32 790 32 555 32 3245
Cuts at: 6226 6258 6300 6332 1373
Size: 32 42 32 2626
Fragments arranged by size:
3245 2626 790 555 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 4929 5175 669
Size: 988 281 1245 1746 246 3079
Fragments arranged by size:
3079 1746 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3573 748
Size: 2825 4760
HpaI GTT'AAC
Cuts at: 3183 5175 3183
Size: 1992 5593
KpnI G_GTAC'C
Cuts at: 4910 4910
Size: 7585
MfeI C'AATT_G
Cuts at: 5184 5184
Size: 7585
MluI A'CGCG_T
Cuts at: 7461 7461
Size: 7585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3373 4125 4450 6699 6883 1305
Size: 2068 752 325 2249 184 2007
Fragments arranged by size:
2249 2068 2007 752 325 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5763
Fragments arranged by size:
5763 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 5543 5902 6061 518
Size: 648 972 3405 359 159 2042
Fragments arranged by size:
3405 2042 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3916 4837 5001 5678 6826 7071 1606
Size: 2310 921 164 677 1148 245 2120
Fragments arranged by size:
2310 2120 1148 921 677 245 164
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7033
NdeI CA'TA_TG
Cuts at: 387 2335 387
Size: 1948 5637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7585
NotI GC'GGCC_GC
Cuts at: 5032 5032
Size: 7585
NspI r_CATG'y
Cuts at: 1132 1229 2785 3114 3625 4489 4517 4582 4943
Size: 97 1556 329 511 864 28 65 361
Cuts at: 4943 6488 1132
Size: 1545 2229
Fragments arranged by size:
2229 1556 1545 864 511 361 329 97
65 28
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7585
PciI A'CATG_T
Cuts at: 2781 3110 4513 6484 2781
Size: 329 1403 1971 3882
Fragments arranged by size:
3882 1971 1403 329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7585
PmeI GTTT'AAAC
Cuts at: 4889 4889
Size: 7585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5654 5686 7167 7199 5654
Size: 32 1481 32 6040
Fragments arranged by size:
6040 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 4841 2055
Size: 2786 4799
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7585
PsiI TTA'TAA
Cuts at: 4647 5155 4647
Size: 508 7077
PspXI vC'TCGA_Gb
Cuts at: 3381 3381
Size: 7585
PstI C_TGCA'G
Cuts at: 830 4450 4937 830
Size: 3620 487 3478
Fragments arranged by size:
3620 3478 487
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7585
PvuII CAG'CTG
Cuts at: 3916 4837 3916
Size: 921 6664
SalI G'TCGA_C
Cuts at: 1936 4927 1936
Size: 2991 4594
SanDI GG'GwC_CC
Cuts at: 2055 2055
Size: 7585
SbfI CC_TGCA'GG
Cuts at: 4937 4937
Size: 7585
ScaI AGT'ACT
Cuts at: 5833 7545 5833
Size: 1712 5873
SfcI C'TryA_G
Cuts at: 826 1045 2092 2182 3327 4167 4446 4933 6068
Size: 219 1047 90 1145 840 279 487 1135
Cuts at: 6068 6749 6940 826
Size: 681 191 1471
Fragments arranged by size:
1471 1145 1135 1047 840 681 487 279
219 191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7585
SmaI CCC'GGG
Cuts at: 1872 4912 1872
Size: 3040 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3303 3381 5689 6590 6852 820
Size: 197 926 1360 78 2308 901 262 1553
Fragments arranged by size:
2308 1553 1360 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 4209 5376 493
Size: 3716 1167 2702
Fragments arranged by size:
3716 2702 1167
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7585
SphI G_CATG'C
Cuts at: 1132 4943 1132
Size: 3811 3774
SspI AAT'ATT
Cuts at: 5 52 3082 5509 5
Size: 47 3030 2427 2081
Fragments arranged by size:
3030 2427 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7585
StyI C'CwwG_G
Cuts at: 513 1065 2289 2943 3006 3249 5024 513
Size: 552 1224 654 63 243 1775 3074
Fragments arranged by size:
3074 1775 1224 654 552 243 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 4799 5612 5629 5782 5967 6412
Size: 1255 690 2529 813 17 153 185 445
Cuts at: 6412 325
Size: 1498
Fragments arranged by size:
2529 1498 1255 813 690 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2605 2798 4308
Size: 274 80 33 51 1443 628 193 1510
Cuts at: 4308 4575 5831 7543 96
Size: 267 1256 1712 138
Fragments arranged by size:
1712 1510 1443 1256 628 274 267 193
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 7545 7577 2480
Size: 32 5033 32 2488
Fragments arranged by size:
5033 2488 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2963 1316
Size: 1647 5938
XbaI T'CTAG_A
Cuts at: 4921 4921
Size: 7585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4841 4841
Size: 7585
XhoI C'TCGA_G
Cuts at: 1943 3381 1943
Size: 1438 6147
XmaI C'CCGG_G
Cuts at: 1870 4910 1870
Size: 3040 4545
XmnI GAAnn'nnTTC
Cuts at: 4735 5714 4735
Size: 979 6606
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5393 276
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI