Restriction map for
pFN21AE1571
(Circular) (Six-base) MAPSORT of: pFN21AE1571.seq Check: 5884 from: 1 to: 5485
pFN21AE1571 5485 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2485 3548 4932 5200 1632
Size: 853 1063 1384 268 1917
Fragments arranged by size:
1917 1384 1063 853 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2529 1184
Size: 1345 4140
AlwNI CAG_nnn'CTG
Cuts at: 2410 2491 3228 4800 2410
Size: 81 737 1572 3095
Fragments arranged by size:
3095 1572 737 81
ApaI G_GGCC'C
Cuts at: 2629 2629
Size: 5485
ApaLI G'TGCA_C
Cuts at: 2353 3542 4698 2353
Size: 1189 1156 3140
Fragments arranged by size:
3140 1189 1156
ApoI r'AATT_y
Cuts at: 1493 2794 3020 3172 5281 1493
Size: 1301 226 152 2109 1697
Fragments arranged by size:
2109 1697 1301 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2810 1870
Size: 73 867 4545
Fragments arranged by size:
4545 867 73
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2806 4261 618
Size: 325 217 627 1019 1455 1842
Fragments arranged by size:
1842 1455 1019 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2058 2107 2417 2629 2804 1391
Size: 667 49 310 212 175 4072
Fragments arranged by size:
4072 667 310 212 175 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2267 928
Size: 971 368 4146
Fragments arranged by size:
4146 971 368
BbvCI CC'TCA_GC
Cuts at: 2424 2424
Size: 5485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4096 136
Size: 107 122 71 631 605 2424 1525
Fragments arranged by size:
2424 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2902 2902
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 1790 2357 2481 2629 3546 4702 1790
Size: 567 124 148 917 1156 2573
Fragments arranged by size:
2573 1156 917 567 148 124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2110 3216 4174
Size: 416 53 83 399 24 693 1106 958
Cuts at: 4174 442
Size: 1753
Fragments arranged by size:
1753 1106 958 693 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2480 4145 1239
Size: 813 428 1665 2579
Fragments arranged by size:
2579 1665 813 428
Bpu10I CC'TnA_GC
Cuts at: 2106 2411 2424 2106
Size: 305 13 5167
Fragments arranged by size:
5167 305 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3610 4475 4773 3610
Size: 865 298 4322
Fragments arranged by size:
4322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2215 4148 882
Size: 617 716 1933 2219
Fragments arranged by size:
2219 1933 716 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3292 3674 4309 275
Size: 53 83 186 936 1759 382 635 1451
Fragments arranged by size:
1759 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2764 3918 4316 4590 4737 5148 2764
Size: 1154 398 274 147 411 3101
Fragments arranged by size:
3101 1154 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5260
Fragments arranged by size:
5260 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2735 2738 1186
Size: 1549 3 3933
Fragments arranged by size:
3933 1549 3
BseYI C'CCAG_C
Cuts at: 2474 4688 5184 2474
Size: 2214 496 2775
Fragments arranged by size:
2775 2214 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2275 2359 2578 1163
Size: 573 539 84 219 4070
Fragments arranged by size:
4070 573 539 219 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2399 2935 3696 4724 1540
Size: 275 186 398 536 761 1028 2301
Fragments arranged by size:
2301 1028 761 536 398 275 186
BsiHKAI G_wGCw'C
Cuts at: 2357 3546 3631 4702 2357
Size: 1189 85 1071 3140
Fragments arranged by size:
3140 1189 1071 85
BsmI GAATG_Cn'
Cuts at: 2996 3089 2996
Size: 93 5392
BsmBI CGTCTCn'nnnn_
Cuts at: 2406 2406
Size: 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2107 2357 2417 2481 2629 2804
Size: 399 268 49 250 60 64 148 175
Cuts at: 2804 3546 3631 4702 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 268 250 175 148
85 64 60 49
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2840 844
Size: 1996 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2393 3372 1394
Size: 429 570 979 3507
Fragments arranged by size:
3507 979 570 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2332 3980 4154 65
Size: 1183 1084 1648 174 1396
Fragments arranged by size:
1648 1396 1183 1084 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4129 4316 1700
Size: 110 2319 187 2869
Fragments arranged by size:
2869 2319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 2150 3545 4557 2150
Size: 1395 1012 3078
Fragments arranged by size:
3078 1395 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5485
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstXI CCAn_nnnn'nTGG
Cuts at: 2022 2022
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2549 2815 3182 3567 3584 5036 5122 5134
Size: 577 266 367 385 17 1452 86 12
Cuts at: 5134 5480 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 577 385 367 346 266 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3994
Fragments arranged by size:
3994 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 2298 2342 3001 3795 3815 2298
Size: 44 659 794 20 3968
Fragments arranged by size:
3968 794 659 44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DraIII CAC_nnn'GTG
Cuts at: 2361 2361
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2395 4492 809
Size: 507 1079 2097 1802
Fragments arranged by size:
2097 1802 1079 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2537 2619
Size: 54 1425 50 275 18 182 525 82
Cuts at: 2619 2932 3821 5450 8
Size: 313 889 1629 43
Fragments arranged by size:
1629 1425 889 525 313 275 182 82
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 3415 1353
Size: 1023 1039 3423
Fragments arranged by size:
3423 1039 1023
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4072 4446 4592 387
Size: 1064 138 2483 374 146 1280
Fragments arranged by size:
2483 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2480 2485 3548 4145 4932 5200
Size: 393 420 428 5 1063 597 787 268
Cuts at: 5200 1239
Size: 1524
Fragments arranged by size:
1524 1063 787 597 428 420 393 268
5
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 5485
EcoO109I rG'GnC_Cy
Cuts at: 2043 2407 2625 2626 2043
Size: 364 218 1 4902
Fragments arranged by size:
4902 364 218 1
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 4632 1678
Size: 2954 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4126 4158 4200 4232 1373
Size: 32 135 32 2554 32 42 32 2626
Fragments arranged by size:
2626 2554 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3075 669
Size: 988 281 891 246 3079
Fragments arranged by size:
3079 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4599 4783 1305
Size: 3294 184 2007
Fragments arranged by size:
3294 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2539 10
Size: 54 1425 343 707 2956
Fragments arranged by size:
2956 1425 707 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 3443 3802 3961 518
Size: 648 1416 861 359 159 2042
Fragments arranged by size:
2042 1416 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2474 2509 2709 2901 3578 4726 4971 1606
Size: 868 35 200 192 677 1148 245 2120
Fragments arranged by size:
2120 1148 868 677 245 200 192 35
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3994
Fragments arranged by size:
3994 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2485 2843 4388 1132
Size: 97 1256 358 1545 2229
Fragments arranged by size:
2229 1545 1256 358 97
PasI CC'CwG_GG
Cuts at: 1348 2715 1348
Size: 1367 4118
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5485
PfoI T'CCnGG_A
Cuts at: 2745 2745
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2043 2043
Size: 5485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PspOMI G'GGCC_C
Cuts at: 2625 2625
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2692 2837 830
Size: 1862 145 3478
Fragments arranged by size:
3478 1862 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
PvuII CAG'CTG
Cuts at: 2474 2509 2709 2474
Size: 35 200 5250
Fragments arranged by size:
5250 200 35
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SanDI GG'GwC_CC
Cuts at: 2043 2043
Size: 5485
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2688 2833 3968 4649 4840 826
Size: 219 1643 145 1135 681 191 1471
Fragments arranged by size:
1643 1471 1135 681 219 191 145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3589 4490 4752 820
Size: 197 926 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2004 2924 513
Size: 552 939 920 3074
Fragments arranged by size:
3074 939 920 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2385 2447 3512 3529 3682 3867 4312
Size: 1255 805 62 1065 17 153 185 445
Cuts at: 4312 325
Size: 1498
Fragments arranged by size:
1498 1255 1065 805 445 185 153 62
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3731 5443 96
Size: 274 80 33 51 1443 1754 1712 138
Fragments arranged by size:
1754 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2336 2368 5445 5477 2336
Size: 32 3077 32 2344
Fragments arranged by size:
3077 2344 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 2022
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI