Restriction map for
pFN21AE1570
(Circular) (Six-base) MAPSORT of: pFN21AE1570.seq Check: 3378 from: 1 to: 6733
pFN21AE1570 6733 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3132 3268 3656 3132
Size: 136 388 6209
Fragments arranged by size:
6209 388 136
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4543 278
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3586 4076 1937
Size: 1649 490 4594
Fragments arranged by size:
4594 1649 490
Acc65I G'GTAC_C
Cuts at: 4054 4054
Size: 6733
AclI AA'CG_TT
Cuts at: 1563 4860 5233 1563
Size: 3297 373 3063
Fragments arranged by size:
3297 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2414 4796 6180 6448 1632
Size: 782 2382 1384 268 1917
Fragments arranged by size:
2382 1917 1384 782 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6536
AflIII A'CryG_T
Cuts at: 1203 3803 5632 6609 1203
Size: 2600 1829 977 1327
Fragments arranged by size:
2600 1829 1327 977
AgeI A'CCGG_T
Cuts at: 5564 5564
Size: 6733
AhdI GACnn_n'nnGTC
Cuts at: 2068 5462 2068
Size: 3394 3339
AleI CACnn'nnGTG
Cuts at: 1166 3635 1166
Size: 2469 4264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2724 3746 1184
Size: 1540 1022 4171
Fragments arranged by size:
4171 1540 1022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2249 2281 4010 4042 2249
Size: 32 1729 32 4940
Fragments arranged by size:
4940 1729 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2332 2678 2731 4476 6048 2332
Size: 346 53 1745 1572 3017
Fragments arranged by size:
3017 1745 1572 346 53
ApaI G_GGCC'C
Cuts at: 2247 2247
Size: 6733
ApaLI G'TGCA_C
Cuts at: 2210 4790 5946 2210
Size: 2580 1156 2997
Fragments arranged by size:
2997 2580 1156
ApoI r'AATT_y
Cuts at: 1493 4042 4268 4420 6529 1493
Size: 2549 226 152 2109 1697
Fragments arranged by size:
2549 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2913 5287 160
Size: 872 1881 2374 1606
Fragments arranged by size:
2374 1881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 3252 3390 3606 3946 4058 1870
Size: 73 148 1161 138 216 340 112 4545
Fragments arranged by size:
4545 1161 340 216 148 138 112 73
BamHI G'GATC_C
Cuts at: 4063 4430 4063
Size: 367 6366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 3866 4054 5509 618
Size: 325 217 627 431 1648 188 1455 1842
Fragments arranged by size:
1842 1648 1455 627 431 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2105 2247 2520 2622 2861 2966 3528 3581
Size: 714 142 273 102 239 105 562 53
Cuts at: 3581 4052 1391
Size: 471 4072
Fragments arranged by size:
4072 714 562 471 273 239 142 105
102 53
BbeI G_GCGC'C
Cuts at: 2222 2222
Size: 6733
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2514 3145 3408 928
Size: 971 615 631 263 4253
Fragments arranged by size:
4253 971 631 615 263
BbvCI CC'TCA_GC
Cuts at: 2151 2151
Size: 6733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4924 4958 1903
Size: 34 2987 34 3678
Fragments arranged by size:
3678 2987 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4625 5835 4625
Size: 1210 5523
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6733
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 2807 5344
Size: 107 122 71 631 605 376 759 2537
Cuts at: 5344 136
Size: 1525
Fragments arranged by size:
2537 1525 759 631 605 376 122 107
71
BglII A'GATC_T
Cuts at: 3546 3942 6728 3546
Size: 396 2786 3551
Fragments arranged by size:
3551 2786 396
BlpI GC'TnA_GC
Cuts at: 2679 2844 3002 3652 3833 4150 2679
Size: 165 158 650 181 317 5262
Fragments arranged by size:
5262 650 317 181 165 158
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2247 3869 4794 5950 1790
Size: 424 33 1622 925 1156 2573
Fragments arranged by size:
2573 1622 1156 925 424 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6733
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2219 2719 4464
Size: 416 53 83 399 24 802 500 1745
Cuts at: 4464 5422 442
Size: 958 1753
Fragments arranged by size:
1753 1745 958 802 500 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6733
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 3568 2875
Size: 693 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2390 2644 3512 3620 3867 5393 1239
Size: 1115 36 254 868 108 247 1526 2579
Fragments arranged by size:
2579 1526 1115 868 254 247 108 36
Bpu10I CC'TnA_GC
Cuts at: 2151 3580 2151
Size: 1429 5304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4858 5723 6021 4858
Size: 865 298 5570
Fragments arranged by size:
5570 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2744 3596 3843 5396 882
Size: 617 1245 852 247 1553 2219
Fragments arranged by size:
2219 1553 1245 852 617 247
BsaAI yAC'GTr
Cuts at: 493 1204 4524 493
Size: 711 3320 2702
Fragments arranged by size:
3320 2702 711
BsaBI GATnn'nnATC
Cuts at: 2591 6727 2591
Size: 4136 2597
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2219 4540 4922 5557
Size: 53 83 186 936 686 2321 382 635
Cuts at: 5557 275
Size: 1451
Fragments arranged by size:
2321 1451 936 686 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2033 2661 3384 3874 5166 5564 5838 5985 6396
Size: 628 723 490 1292 398 274 147 411
Cuts at: 6396 2033
Size: 2370
Fragments arranged by size:
2370 1292 723 628 490 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4010 4040 1178
Size: 30 165 30 2607 30 3871
Fragments arranged by size:
3871 2607 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2300 2438 2641 3408 3583 3908 1186
Size: 982 132 138 203 767 175 325 4011
Fragments arranged by size:
4011 982 767 325 203 175 138 132
BseYI C'CCAG_C
Cuts at: 2239 2671 3719 5936 6432 2239
Size: 432 1048 2217 496 2540
Fragments arranged by size:
2540 2217 1048 496 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3113 3218 3471 3641 3696 1163
Size: 573 1377 105 253 170 55 4200
Fragments arranged by size:
4200 1377 573 253 170 105 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4183 4944 5972 1540
Size: 275 186 2182 761 1028 2301
Fragments arranged by size:
2301 2182 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 2406 2520 2685 2697 3528 3581 3885 4794
Size: 192 114 165 12 831 53 304 909
Cuts at: 4794 4879 5950 2214
Size: 85 1071 2997
Fragments arranged by size:
2997 1071 909 831 304 192 165 114
85 53 12
BsmI GAATG_Cn'
Cuts at: 2551 4244 4337 2551
Size: 1693 93 4947
Fragments arranged by size:
4947 1693 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4030 4030
Size: 6733
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2214 2247 2406 2520 2622 2685
Size: 399 315 109 33 159 114 102 63
Cuts at: 2685 2697 2861 2966 3528 3581 3869 3885 4052
Size: 12 164 105 562 53 288 16 167
Cuts at: 4052 4794 4879 5950 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 399 315 288 167
164 159 114 109 105 102 85 63
53 33 16 12
BspEI T'CCGG_A
Cuts at: 2661 3384 3874 6396 2661
Size: 723 490 2522 2998
Fragments arranged by size:
2998 2522 723 490
BspHI T'CATG_A
Cuts at: 4622 6352 4622
Size: 1730 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3132 3268 3656 3720 4088 844
Size: 2288 136 388 64 368 3489
Fragments arranged by size:
3489 2288 388 368 136 64
BsrBI CCG'CTC
Cuts at: 1394 1823 3964 4620 1394
Size: 429 2141 656 3507
Fragments arranged by size:
3507 2141 656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3094 5228 5402 65
Size: 1183 1846 2134 174 1396
Fragments arranged by size:
2134 1846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5377 5564 1700
Size: 110 3567 187 2869
Fragments arranged by size:
3567 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6733
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6733
BssSI C'ACGA_G
Cuts at: 4793 5805 4793
Size: 1012 5721
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3127 794
Size: 2333 4400
BstBI TT'CG_AA
Cuts at: 5537 5537
Size: 6733
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6733
BstXI CCAn_nnnn'nTGG
Cuts at: 3754 3754
Size: 6733
BstYI r'GATC_y
Cuts at: 1972 2649 3546 3942 4063 4430 4815 4832 6284
Size: 677 897 396 121 367 385 17 1452
Cuts at: 6284 6370 6382 6728 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 897 677 396 385 367 346
121 86 17 12
BstZ17I GTA'TAC
Cuts at: 3587 3587
Size: 6733
Bsu36I CC'TnA_GG
Cuts at: 3147 3375 3147
Size: 228 6505
BtgI C'CryG_G
Cuts at: 513 1065 2004 2294 2802 3633 513
Size: 552 939 290 508 831 3613
Fragments arranged by size:
3613 939 831 552 508 290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3898 487
Size: 683 56 2672 3322
Fragments arranged by size:
3322 2672 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2139 2482 3184 3459 4249 5043 5063 2108
Size: 31 343 702 275 790 794 20 3778
Fragments arranged by size:
3778 794 790 702 343 275 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6733
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6698
DraI TTT'AAA
Cuts at: 4037 4037
Size: 6733
DraIII CAC_nnn'GTG
Cuts at: 2401 2401
Size: 6733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5740 809
Size: 507 4424 1802
Fragments arranged by size:
4424 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2302 3069
Size: 54 1425 50 275 18 177 295 767
Cuts at: 3069 4180 5069 6698 8
Size: 1111 889 1629 43
Fragments arranged by size:
1629 1425 1111 889 767 295 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4180 1537
Size: 275 2368 4090
Fragments arranged by size:
4090 2368 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 3203 4663 1353
Size: 965 885 1460 3423
Fragments arranged by size:
3423 1460 965 885
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2747 3922 5320 5694 5840 387
Size: 1064 138 1158 1175 1398 374 146 1280
Fragments arranged by size:
1398 1280 1175 1158 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2518 3526 3579 2518
Size: 1008 53 5672
Fragments arranged by size:
5672 1008 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2390 2414 2644 3512 3620 3867
Size: 393 722 36 24 230 868 108 247
Cuts at: 3867 4796 5393 6180 6448 1239
Size: 929 597 787 268 1524
Fragments arranged by size:
1524 929 868 787 722 597 393 268
247 230 108 36 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3569 3569
Size: 6733
EcoO109I rG'GnC_Cy
Cuts at: 2088 2243 2244 2346 2667 2822 3112 3858 3898
Size: 155 1 102 321 155 290 746 40
Cuts at: 3898 3973 2088
Size: 75 4848
Fragments arranged by size:
4848 746 321 290 155 155 102 75
40 1
EcoRI G'AATT_C
Cuts at: 4042 4420 4042
Size: 378 6355
EcoRV GAT'ATC
Cuts at: 3382 3382
Size: 6733
FspI TGC'GCA
Cuts at: 3086 5239 3086
Size: 2153 4580
HaeII r_GCGC'y
Cuts at: 1678 2151 2222 2419 5880 1678
Size: 473 71 197 3461 2531
Fragments arranged by size:
3461 2531 473 197 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 5374 5406 5448
Size: 32 135 32 482 32 3288 32 42
Cuts at: 5448 5480 1373
Size: 32 2626
Fragments arranged by size:
3288 2626 482 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4077 4323 669
Size: 988 281 2139 246 3079
Fragments arranged by size:
3079 2139 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6733
HpaI GTT'AAC
Cuts at: 4323 4323
Size: 6733
KasI G'GCGC_C
Cuts at: 2218 2218
Size: 6733
KpnI G_GTAC'C
Cuts at: 4058 4058
Size: 6733
MfeI C'AATT_G
Cuts at: 4332 4332
Size: 6733
MluI A'CGCG_T
Cuts at: 6609 6609
Size: 6733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5847 6031 1305
Size: 4542 184 2007
Fragments arranged by size:
4542 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3071 10
Size: 54 1425 343 1239 3672
Fragments arranged by size:
3672 1425 1239 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3635 4691 5050 5209 518
Size: 648 2469 1056 359 159 2042
Fragments arranged by size:
2469 2042 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2274 2284 2296 2678 2763 2980 3178 3349
Size: 668 10 12 382 85 217 198 171
Cuts at: 3349 3730 4149 4826 5974 6219 1606
Size: 381 419 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 419 382 381 245
217 198 171 85 12 10
NarI GG'CG_CC
Cuts at: 2219 2219
Size: 6733
NcoI C'CATG_G
Cuts at: 513 1065 2004 2802 3633 513
Size: 552 939 798 831 3613
Fragments arranged by size:
3613 939 831 798 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6733
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6733
NotI GC'GGCC_GC
Cuts at: 4180 4180
Size: 6733
NspI r_CATG'y
Cuts at: 1132 1229 3665 3727 3765 3807 4091 5636 1132
Size: 97 2436 62 38 42 284 1545 2229
Fragments arranged by size:
2436 2229 1545 284 97 62 42 38
PasI CC'CwG_GG
Cuts at: 1348 3862 1348
Size: 2514 4219
PciI A'CATG_T
Cuts at: 3803 5632 3803
Size: 1829 4904
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 3078 1108
Size: 1224 746 4763
Fragments arranged by size:
4763 1224 746
PfoI T'CCnGG_A
Cuts at: 2175 3957 2175
Size: 1782 4951
PmeI GTTT'AAAC
Cuts at: 4037 4037
Size: 6733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 4802 4834 6315 6347 4010
Size: 32 760 32 1481 32 4396
Fragments arranged by size:
4396 1481 760 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2088 2346 2667 2822 2088
Size: 258 321 155 5999
Fragments arranged by size:
5999 321 258 155
PshAI GACnn'nnGTC
Cuts at: 1613 2906 1613
Size: 1293 5440
PsiI TTA'TAA
Cuts at: 4303 4303
Size: 6733
PspOMI G'GGCC_C
Cuts at: 2243 2243
Size: 6733
PstI C_TGCA'G
Cuts at: 830 2361 4085 830
Size: 1531 1724 3478
Fragments arranged by size:
3478 1724 1531
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6733
PvuII CAG'CTG
Cuts at: 2284 2678 3178 3349 3730 2284
Size: 394 500 171 381 5287
Fragments arranged by size:
5287 500 394 381 171
SacI G_AGCT'C
Cuts at: 2520 3528 3581 2520
Size: 1008 53 5672
Fragments arranged by size:
5672 1008 53
SacII CC_GC'GG
Cuts at: 2297 2297
Size: 6733
SalI G'TCGA_C
Cuts at: 1936 4075 1936
Size: 2139 4594
SanDI GG'GwC_CC
Cuts at: 2088 2346 2088
Size: 258 6475
SbfI CC_TGCA'GG
Cuts at: 4085 4085
Size: 6733
ScaI AGT'ACT
Cuts at: 4981 6693 4981
Size: 1712 5021
SfcI C'TryA_G
Cuts at: 826 1045 2357 2561 4081 5216 5897 6088 826
Size: 219 1312 204 1520 1135 681 191 1471
Fragments arranged by size:
1520 1471 1312 1135 681 219 204 191
SfoI GGC'GCC
Cuts at: 2220 2220
Size: 6733
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6733
SmaI CCC'GGG
Cuts at: 1872 2093 3254 3608 4060 1872
Size: 221 1161 354 452 4545
Fragments arranged by size:
4545 1161 452 354 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3946 4837 5738 6000 820
Size: 197 926 2003 891 901 262 1553
Fragments arranged by size:
2003 1553 926 901 891 262 197
SnaBI TAC'GTA
Cuts at: 493 4524 493
Size: 4031 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6733
SphI G_CATG'C
Cuts at: 1132 3665 3727 4091 1132
Size: 2533 62 364 3774
Fragments arranged by size:
3774 2533 364 62
SspI AAT'ATT
Cuts at: 5 52 4657 5
Size: 47 4605 2081
Fragments arranged by size:
4605 2081 47
StuI AGG'CCT
Cuts at: 1517 3533 3753 1517
Size: 2016 220 4497
Fragments arranged by size:
4497 2016 220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2373 2802 3555 3633 4172 513
Size: 552 939 369 429 753 78 539 3074
Fragments arranged by size:
3074 939 753 552 539 429 369 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3070 3733 3754 4760 4777 4930 5115
Size: 1255 1490 663 21 1006 17 153 185
Cuts at: 5115 5560 325
Size: 445 1498
Fragments arranged by size:
1498 1490 1255 1006 663 445 185 153
21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4979 6691 96
Size: 274 80 33 51 1443 3002 1712 138
Fragments arranged by size:
3002 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6693 6725 6693
Size: 32 6701
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6733
XbaI T'CTAG_A
Cuts at: 4069 4069
Size: 6733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2732 2732
Size: 6733
XhoI C'TCGA_G
Cuts at: 1943 3946 1943
Size: 2003 4730
XmaI C'CCGG_G
Cuts at: 1870 2091 3252 3606 4058 1870
Size: 221 1161 354 452 4545
Fragments arranged by size:
4545 1161 452 354 221
XmnI GAAnn'nnTTC
Cuts at: 4862 4862
Size: 6733
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4541 276
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SapI SexAI SfiI SrfI SwaI