Restriction map for
pFN21AE1567
(Circular) (Six-base) MAPSORT of: pFN21AE1567.seq Check: 9057 from: 1 to: 5959
pFN21AE1567 5959 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2349 2616 3302 1937
Size: 412 267 686 4594
Fragments arranged by size:
4594 686 412 267
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 3261 4022 5406 5674 1632
Size: 514 1115 761 1384 268 1917
Fragments arranged by size:
1917 1384 1115 761 514 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 2154 4858 5835 1203
Size: 951 2704 977 1327
Fragments arranged by size:
2704 1327 977 951
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 2543 1166
Size: 1377 4582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2204 2414 1184
Size: 1020 210 4729
Fragments arranged by size:
4729 1020 210
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaI G_GGCC'C
Cuts at: 2746 2746
Size: 5959
ApaLI G'TGCA_C
Cuts at: 2389 4016 5172 2389
Size: 1627 1156 3176
Fragments arranged by size:
3176 1627 1156
ApoI r'AATT_y
Cuts at: 1493 2091 2507 2765 3268 3494 3646 5755 1493
Size: 598 416 258 503 226 152 2109 1697
Fragments arranged by size:
2109 1697 598 503 416 258 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 2367 3289 3656 2367
Size: 922 367 4670
Fragments arranged by size:
4670 922 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3042 3280 4735 618
Size: 325 217 627 1255 238 1455 1842
Fragments arranged by size:
1842 1455 1255 627 325 238 217
BanII G_rGCy'C
Cuts at: 1391 2220 2556 2746 3172 3278 1391
Size: 829 336 190 426 106 4072
Fragments arranged by size:
4072 829 426 336 190 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2326 2360 4150 4184 1903
Size: 34 50 34 305 34 1790 34 3678
Fragments arranged by size:
3678 1790 305 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2280 1548
Size: 732 5227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2843 4570 136
Size: 107 122 71 631 605 1171 1727 1525
Fragments arranged by size:
1727 1525 1171 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3137 3376 3137
Size: 239 5720
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2393 2746 3047 4020 5176 1790
Size: 403 200 353 301 973 1156 2573
Fragments arranged by size:
2573 1156 973 403 353 301 200
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2491 2035
Size: 456 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2193 2234 3212 4619 1239
Size: 954 41 978 1407 2579
Fragments arranged by size:
2579 1407 978 954 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2539 3096 4622 882
Size: 617 1040 557 1526 2219
Fragments arranged by size:
2219 1526 1040 617 557
BsaAI yAC'GTr
Cuts at: 493 1204 2029 2188 2673 3750 493
Size: 711 825 159 485 1077 2702
Fragments arranged by size:
2702 1077 825 711 485 159
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2364 4392 4790 5064 5211 5622 2364
Size: 2028 398 274 147 411 2701
Fragments arranged by size:
2701 2028 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 2064 2521 5162 5658 2064
Size: 457 2641 496 2365
Fragments arranged by size:
2641 2365 496 457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3137 1163
Size: 573 1401 3985
Fragments arranged by size:
3985 1401 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2544 3409 4170 5198 1540
Size: 275 186 543 865 761 1028 2301
Fragments arranged by size:
2301 1028 865 761 543 275 186
BsiHKAI G_wGCw'C
Cuts at: 2393 4020 4105 5176 2393
Size: 1627 85 1071 3176
Fragments arranged by size:
3176 1627 1071 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2220 2393 2556 2746 3047 3172
Size: 399 403 27 173 163 190 301 125
Cuts at: 3172 3278 4020 4105 5176 1391
Size: 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 301 190 173
163 125 106 85 27
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 3846 1394
Size: 429 647 1376 3507
Fragments arranged by size:
3507 1376 647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2062 4454 4628 65
Size: 1183 814 2392 174 1396
Fragments arranged by size:
2392 1396 1183 814 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 2958 96
Size: 2862 3097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 2852 4019 5031 2852
Size: 1167 1012 3780
Fragments arranged by size:
3780 1167 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2061 794
Size: 1267 4692
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 2426 2545 2908 2426
Size: 119 363 5477
Fragments arranged by size:
5477 363 119
BstYI r'GATC_y
Cuts at: 1972 2367 2383 3289 3656 4041 4058 5510 5596
Size: 395 16 906 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 906 395 385 367 346 86
17 16 12
BtgI C'CryG_G
Cuts at: 513 1065 2538 2544 2865 2929 3084 3147 3225
Size: 552 1473 6 321 64 155 63 78
Cuts at: 3225 513
Size: 3247
Fragments arranged by size:
3247 1473 552 321 155 78 64 63
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2983 3139 487
Size: 683 56 1757 156 3307
Fragments arranged by size:
3307 1757 683 156 56
BtsI GCAGTG_nn'
Cuts at: 2205 3475 4269 4289 2205
Size: 1270 794 20 3875
Fragments arranged by size:
3875 1270 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2541 2811
Size: 54 1425 50 275 18 471 240 270
Cuts at: 2811 2994 3108 3150 3406 4295 5924 8
Size: 183 114 42 256 889 1629 43
Fragments arranged by size:
1629 1425 889 471 275 270 256 240
183 114 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2541 3406 1537
Size: 275 729 865 4090
Fragments arranged by size:
4090 865 729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 3201 3889 1353
Size: 914 934 688 3423
Fragments arranged by size:
3423 934 914 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2396 4546 4920 5066 387
Size: 1064 138 807 2150 374 146 1280
Fragments arranged by size:
2150 1280 1064 807 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2193 2234 3212 3261 4022 4619
Size: 393 514 47 41 978 49 761 597
Cuts at: 4619 5406 5674 1239
Size: 787 268 1524
Fragments arranged by size:
1524 978 787 761 597 514 393 268
49 47 41
EcoNI CCTnn'n_nnAGG
Cuts at: 3110 3110
Size: 5959
EcoO109I rG'GnC_Cy
Cuts at: 2742 2743 2742
Size: 1 5958
EcoRI G'AATT_C
Cuts at: 2091 2765 3268 3646 2091
Size: 674 503 378 4404
Fragments arranged by size:
4404 674 503 378
FspI TGC'GCA
Cuts at: 2240 4465 2240
Size: 2225 3734
FspAI rTGC'GCAy
Cuts at: 2240 2240
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 2846 2878 4600
Size: 32 135 32 462 32 780 32 1722
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1722 780 462 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 2617 2989 3303 3549 669
Size: 988 281 412 267 372 314 246 3079
Fragments arranged by size:
3079 988 412 372 314 281 267 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2813 3110 10
Size: 54 1425 343 981 297 2859
Fragments arranged by size:
2859 1425 981 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 3917 4276 4435 518
Size: 648 1377 1374 359 159 2042
Fragments arranged by size:
2042 1377 1374 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2278 2860 3121 3375 4052 5200 5445 1606
Size: 672 582 261 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 582 261 254 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 2639 387
Size: 2252 3707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2956 3136 3317 4862 1132
Size: 97 1727 180 181 1545 2229
Fragments arranged by size:
2229 1727 1545 181 180 97
PasI CC'CwG_GG
Cuts at: 1348 3095 1348
Size: 1747 4212
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2358 2358
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PmlI CAC'GTG
Cuts at: 2029 2188 2029
Size: 159 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2640 1613
Size: 1027 4932
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2742 2742
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2403 3311 830
Size: 1573 908 3478
Fragments arranged by size:
3478 1573 908
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2278 2860 2278
Size: 582 5377
SalI G'TCGA_C
Cuts at: 1936 2348 2615 3301 1936
Size: 412 267 686 4594
Fragments arranged by size:
4594 686 412 267
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SexAI A'CCwGG_T
Cuts at: 2352 2352
Size: 5959
SfcI C'TryA_G
Cuts at: 826 1045 2251 2399 3307 4442 5123 5314 826
Size: 219 1206 148 908 1135 681 191 1471
Fragments arranged by size:
1471 1206 1135 908 681 219 191 148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3136 3317 1132
Size: 2004 181 3774
Fragments arranged by size:
3774 2004 181
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2846 3164 1517
Size: 1329 318 4312
Fragments arranged by size:
4312 1329 318
StyI C'CwwG_G
Cuts at: 513 1065 3111 3398 513
Size: 552 2046 287 3074
Fragments arranged by size:
3074 2046 552 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2283 2591 3986 4003 4156 4341 4786
Size: 1255 703 308 1395 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1395 1255 703 445 308 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 2937 2958
Size: 274 80 33 51 1443 96 864 21
Cuts at: 2958 4205 5917 96
Size: 1247 1712 138
Fragments arranged by size:
1712 1443 1247 864 274 138 96 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3045 3077 5919 5951 3045
Size: 32 2842 32 3053
Fragments arranged by size:
3053 2842 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2354 1316
Size: 1038 4921
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2545 2545
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI