Restriction map for
pFN21AE1566
(Circular) (Six-base) MAPSORT of: pFN21AE1566.seq Check: 3967 from: 1 to: 6130
pFN21AE1566 6130 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 2611 3451 2611
Size: 840 5290
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2936 3234 4193 5577 5845 1632
Size: 1304 298 959 1384 268 1917
Fragments arranged by size:
1917 1384 1304 959 298 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 2023 2230 2684 3873 5445 2023
Size: 207 454 1189 1572 2708
Fragments arranged by size:
2708 1572 1189 454 207
ApaI G_GGCC'C
Cuts at: 2059 2059
Size: 6130
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3439 3665 3817 5926 1493
Size: 1946 226 152 2109 1697
Fragments arranged by size:
2109 1946 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 3455 1870
Size: 73 98 1414 4545
Fragments arranged by size:
4545 1414 98 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2611 3451 4906 618
Size: 325 217 627 824 840 1455 1842
Fragments arranged by size:
1842 1455 840 824 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2059 2143 2669 2906 3235 3449 1391
Size: 668 84 526 237 329 214 4072
Fragments arranged by size:
4072 668 526 329 237 214 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BfrBI ATG'CAT
Cuts at: 3418 3418
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2059 4191 5347 1790
Size: 269 2132 1156 2573
Fragments arranged by size:
2573 2132 1156 269
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2817 3390 3861
Size: 416 53 83 399 24 1400 573 471
Cuts at: 3861 4819 442
Size: 958 1753
Fragments arranged by size:
1753 1400 958 573 471 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 2589 2609 3141 4790 1239
Size: 1128 222 20 532 1649 2579
Fragments arranged by size:
2579 1649 1128 532 222 20
Bpu10I CC'TnA_GC
Cuts at: 2176 2176
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4255 5120 5418 4255
Size: 865 298 4967
Fragments arranged by size:
4967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2626 3921 493
Size: 711 1422 1295 2702
Fragments arranged by size:
2702 1422 1295 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2920 3937 4319 4954
Size: 53 83 186 936 1387 1017 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1451 1387 1017 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2481 2497 2527 2743
Size: 30 165 30 1048 30 16 30 216
Cuts at: 2743 2773 2800 2830 3082 3112 1178
Size: 30 27 30 252 30 4196
Fragments arranged by size:
4196 1048 252 216 165 30 30 30
30 30 30 30 27 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2808 2997 3000 3003 3027 3138 3141 3144
Size: 1622 189 3 3 24 111 3 3
Cuts at: 3144 3188 1186
Size: 44 4128
Fragments arranged by size:
4128 1622 189 111 44 24 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2247 5333 5829 2247
Size: 3086 496 2548
Fragments arranged by size:
3086 2548 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4191 4276 5347 4191
Size: 85 1071 4974
Fragments arranged by size:
4974 1071 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2143 2669 2906 3235 3449 4191
Size: 399 269 84 526 237 329 214 742
Cuts at: 4191 4276 5347 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 329 269 237
214 85 84
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2845 96
Size: 2749 3381
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 2186 794
Size: 1229 163 4738
Fragments arranged by size:
4738 1229 163
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125
Size: 1488 367 385 17 1452 86 12 346
Cuts at: 6125 1972
Size: 1977
Fragments arranged by size:
1977 1488 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3227 513
Size: 552 939 1223 3416
Fragments arranged by size:
3416 1223 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2722 3646 4440 4460 2722
Size: 924 794 20 4392
Fragments arranged by size:
4392 924 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2208 3063
Size: 54 1425 50 275 18 261 117 855
Cuts at: 3063 3577 4466 6095 8
Size: 514 889 1629 43
Fragments arranged by size:
1629 1425 889 855 514 275 261 117
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 2066 2317 3165 3210 4060 1353
Size: 672 41 251 848 45 850 3423
Fragments arranged by size:
3423 850 848 672 251 45 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4717 5091 5237 387
Size: 1064 138 435 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 2589 2609 2936 3141 3234 4193
Size: 393 735 222 20 327 205 93 959
Cuts at: 4193 4790 5577 5845 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 959 787 735 597 393 327 268
222 205 93 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2544 2544
Size: 6130
EcoO109I rG'GnC_Cy
Cuts at: 2056 2972 3220 2056
Size: 916 248 4966
Fragments arranged by size:
4966 916 248
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
EcoRV GAT'ATC
Cuts at: 2236 2236
Size: 6130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2576 2576
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 5277 1678
Size: 3599 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2365 2397 2743 2775 3284
Size: 32 135 32 793 32 346 32 509
Cuts at: 3284 3316 4771 4803 4845 4877 1373
Size: 32 1455 32 42 32 2626
Fragments arranged by size:
2626 1455 793 509 346 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 2615 3455 2615
Size: 840 5290
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2540 2729 5244 5428 1305
Size: 1235 189 2515 184 2007
Fragments arranged by size:
2515 2007 1235 189 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 3065 10
Size: 54 1425 343 378 855 3075
Fragments arranged by size:
3075 1425 855 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 4088 4447 4606 518
Size: 648 1320 1602 359 159 2042
Fragments arranged by size:
2042 1602 1320 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3546 4223 5371 5616 1606
Size: 1940 677 1148 245 2120
Fragments arranged by size:
2120 1940 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NsiI A_TGCA'T
Cuts at: 3420 3420
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 3488 5033 1132
Size: 97 2259 1545 2229
Fragments arranged by size:
2259 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2972 3220 2972
Size: 248 5882
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PspOMI G'GGCC_C
Cuts at: 2055 2055
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2019 2226 3482 830
Size: 1189 207 1256 3478
Fragments arranged by size:
3478 1256 1189 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2015 2222 2854 2914 3026 3478 4613
Size: 219 970 207 632 60 112 452 1135
Cuts at: 4613 5294 5485 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 970 681 632 452 219 207
191 112 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 2043 3457 1872
Size: 171 1414 4545
Fragments arranged by size:
4545 1414 171
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4234 5135 5397 820
Size: 197 926 2291 901 262 1553
Fragments arranged by size:
2291 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2626 3921 493
Size: 2133 1295 2702
Fragments arranged by size:
2702 2133 1295
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 3082 4054 5
Size: 47 3030 972 2081
Fragments arranged by size:
3030 2081 972 47
StuI AGG'CCT
Cuts at: 1517 2869 1517
Size: 1352 4778
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 3569 513
Size: 552 939 201 1364 3074
Fragments arranged by size:
3074 1364 939 552 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 2480 2754 4157 4174 4327 4512
Size: 1255 603 297 274 1403 17 153 185
Cuts at: 4512 4957 325
Size: 445 1498
Fragments arranged by size:
1498 1403 1255 603 445 297 274 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2845 4376 6088
Size: 274 80 33 51 1443 868 1531 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
1712 1531 1443 868 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 3212 3244 6090 6122 2451
Size: 32 729 32 2846 32 2459
Fragments arranged by size:
2846 2459 729 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 2440 3466 2440
Size: 1026 5104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 2041 3455 1870
Size: 171 1414 4545
Fragments arranged by size:
4545 1414 171
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI