Restriction map for
pFN21AE1565
(Circular) (Six-base) MAPSORT of: pFN21AE1565.seq Check: 8690 from: 1 to: 6901
pFN21AE1565 6901 bp
With 182 enzymes: SgfI *
January 20, 2017 10:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4711 278
Size: 53 83 186 936 3175 2468
Fragments arranged by size:
3175 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4244 1937
Size: 2307 4594
Acc65I G'GTAC_C
Cuts at: 4222 4222
Size: 6901
AclI AA'CG_TT
Cuts at: 1563 3558 5028 5401 1563
Size: 1995 1470 373 3063
Fragments arranged by size:
3063 1995 1470 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2254 2287 4964 6348 6616 1632
Size: 452 170 33 2677 1384 268 1917
Fragments arranged by size:
2677 1917 1384 452 268 170 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6704
AflIII A'CryG_T
Cuts at: 1203 2544 4153 5800 6777 1203
Size: 1341 1609 1647 977 1327
Fragments arranged by size:
1647 1609 1341 1327 977
AgeI A'CCGG_T
Cuts at: 5732 5732
Size: 6901
AhdI GACnn_n'nnGTC
Cuts at: 5630 5630
Size: 6901
AleI CACnn'nnGTG
Cuts at: 1166 3356 1166
Size: 2190 4711
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6901
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 6869
AlwNI CAG_nnn'CTG
Cuts at: 2679 3802 4644 6216 2679
Size: 1123 842 1572 3364
Fragments arranged by size:
3364 1572 1123 842
ApaLI G'TGCA_C
Cuts at: 4958 6114 4958
Size: 1156 5745
ApoI r'AATT_y
Cuts at: 1493 2474 3088 3452 3707 4210 4436 4588 6697
Size: 981 614 364 255 503 226 152 2109
Cuts at: 6697 1493
Size: 1697
Fragments arranged by size:
2109 1697 981 614 503 364 255 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3395 5455 160
Size: 872 2363 2060 1606
Fragments arranged by size:
2363 2060 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 4226 1870
Size: 73 172 2111 4545
Fragments arranged by size:
4545 2111 172 73
BamHI G'GATC_C
Cuts at: 2837 4231 4598 2837
Size: 1394 367 5140
Fragments arranged by size:
5140 1394 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 4222 5677 618
Size: 325 217 627 323 2112 1455 1842
Fragments arranged by size:
2112 1842 1455 627 325 323 217
BanII G_rGCy'C
Cuts at: 1391 2921 3744 4220 1391
Size: 1530 823 476 4072
Fragments arranged by size:
4072 1530 823 476
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2442 928
Size: 971 543 5387
Fragments arranged by size:
5387 971 543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5092 5126 1903
Size: 34 3155 34 3678
Fragments arranged by size:
3678 3155 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4793 6003 4793
Size: 1210 5691
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6901
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5512 136
Size: 107 122 71 631 605 3840 1525
Fragments arranged by size:
3840 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6896 6896
Size: 6901
BlpI GC'TnA_GC
Cuts at: 4318 4318
Size: 6901
Bme1580I G_kGCm'C
Cuts at: 1790 2115 4962 6118 1790
Size: 325 2847 1156 2573
Fragments arranged by size:
2847 2573 1156 325
BmgBI CAC'GTC
Cuts at: 1318 3730 4174 1318
Size: 2412 444 4045
Fragments arranged by size:
4045 2412 444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2354 4632 5590
Size: 416 53 83 399 24 937 2278 958
Cuts at: 5590 442
Size: 1753
Fragments arranged by size:
2278 1753 958 937 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2701 3758 4038 5561 1239
Size: 1462 1057 280 1523 2579
Fragments arranged by size:
2579 1523 1462 1057 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5026 5891 6189 5026
Size: 865 298 5738
Fragments arranged by size:
5738 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3842 4108 5564 882
Size: 617 2343 266 1456 2219
Fragments arranged by size:
2343 2219 1456 617 266
BsaAI yAC'GTr
Cuts at: 493 1204 2547 4692 493
Size: 711 1343 2145 2702
Fragments arranged by size:
2702 2145 1343 711
BsaBI GATnn'nnATC
Cuts at: 3905 6895 3905
Size: 2990 3911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2865 4708 5090
Size: 53 83 186 936 588 744 1843 382
Cuts at: 5090 5725 275
Size: 635 1451
Fragments arranged by size:
1843 1451 936 744 635 588 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2913 2934 3624 5334 5732 6006 6153 6564 2913
Size: 21 690 1710 398 274 147 411 3250
Fragments arranged by size:
3250 1710 690 411 398 274 147 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2674 2704 3731 3761 1178
Size: 30 165 30 1271 30 1027 30 4318
Fragments arranged by size:
4318 1271 1027 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3432 1186
Size: 2246 4655
BseYI C'CCAG_C
Cuts at: 2949 3592 6104 6600 2949
Size: 643 2512 496 3250
Fragments arranged by size:
3250 2512 643 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 3803 4094 4196 1163
Size: 573 543 1524 291 102 3868
Fragments arranged by size:
3868 1524 573 543 291 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4351 5112 6140 1540
Size: 275 186 2350 761 1028 2301
Fragments arranged by size:
2350 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2743 2921 4962 5047 6118 2743
Size: 178 2041 85 1071 3526
Fragments arranged by size:
3526 2041 1071 178 85
BsmI GAATG_Cn'
Cuts at: 2812 4412 4505 2812
Size: 1600 93 5208
Fragments arranged by size:
5208 1600 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3713 3713
Size: 6901
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2743 2921 3744 4220 4962 5047
Size: 399 325 628 178 823 476 742 85
Cuts at: 5047 6118 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 823 742 628 476 399 325
178 85
BspEI T'CCGG_A
Cuts at: 3624 6564 3624
Size: 2940 3961
BspHI T'CATG_A
Cuts at: 2412 3540 4790 6520 2412
Size: 1128 1250 1730 2793
Fragments arranged by size:
2793 1730 1250 1128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2091 2136 4256 844
Size: 1247 45 2120 3489
Fragments arranged by size:
3489 2120 1247 45
BsrBI CCG'CTC
Cuts at: 1394 1823 4788 1394
Size: 429 2965 3507
Fragments arranged by size:
3507 2965 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2805 5396 5570 65
Size: 1183 1557 2591 174 1396
Fragments arranged by size:
2591 1557 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3779 4092 5545 5732 1700
Size: 110 1969 313 1453 187 2869
Fragments arranged by size:
2869 1969 1453 313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6901
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6901
BssSI C'ACGA_G
Cuts at: 2149 4961 5973 2149
Size: 2812 1012 3077
Fragments arranged by size:
3077 2812 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6901
BstBI TT'CG_AA
Cuts at: 2518 5705 2518
Size: 3187 3714
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6901
BstXI CCAn_nnnn'nTGG
Cuts at: 2880 2880
Size: 6901
BstYI r'GATC_y
Cuts at: 1972 2296 2837 4231 4598 4983 5000 6452 6538
Size: 324 541 1394 367 385 17 1452 86
Cuts at: 6538 6550 6896 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1394 541 385 367 346 324
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3916 4197 513
Size: 552 2851 281 3217
Fragments arranged by size:
3217 2851 552 281
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3231 487
Size: 683 56 2005 4157
Fragments arranged by size:
4157 2005 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 4082 4144 4417 5211 5231 3124
Size: 958 62 273 794 20 4794
Fragments arranged by size:
4794 958 794 273 62 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6901
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3367 3402 3898 3933 529
Size: 35 2803 35 496 35 3497
Fragments arranged by size:
3497 2803 496 35 35 35
DraI TTT'AAA
Cuts at: 3975 4205 3975
Size: 230 6671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2825 5908 809
Size: 507 1509 3083 1802
Fragments arranged by size:
3083 1802 1509 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 3265 3864
Size: 54 1425 50 275 18 1111 324 599
Cuts at: 3864 4348 5237 6866 8
Size: 484 889 1629 43
Fragments arranged by size:
1629 1425 1111 889 599 484 324 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4348 1537
Size: 275 2536 4090
Fragments arranged by size:
4090 2536 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2035 2306 2561 2607 2755 3468 4831 1353
Size: 682 271 255 46 148 713 1363 3423
Fragments arranged by size:
3423 1363 713 682 271 255 148 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5488 5862 6008 387
Size: 1064 138 3899 374 146 1280
Fragments arranged by size:
3899 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2919 2919
Size: 6901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2254 2287 2701 3758 4038 4964
Size: 393 452 170 33 414 1057 280 926
Cuts at: 4964 5561 6348 6616 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1057 926 787 597 452 414 393
280 268 170 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2165 2165
Size: 6901
EcoO109I rG'GnC_Cy
Cuts at: 3694 3694
Size: 6901
EcoRI G'AATT_C
Cuts at: 3088 3707 4210 4588 3088
Size: 619 503 378 5401
Fragments arranged by size:
5401 619 503 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2156 4209 2156
Size: 2053 4848
FspI TGC'GCA
Cuts at: 5407 5407
Size: 6901
HaeII r_GCGC'y
Cuts at: 1678 6048 1678
Size: 4370 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2443 2674 2706 3731
Size: 32 135 32 839 32 231 32 1025
Cuts at: 3731 3763 5542 5574 5616 5648 1373
Size: 32 1779 32 42 32 2626
Fragments arranged by size:
2626 1779 1025 839 231 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 2896 3313 3562 4245 4491
Size: 988 281 187 771 417 249 683 246
Cuts at: 4491 669
Size: 3079
Fragments arranged by size:
3079 988 771 683 417 281 249 246
187
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6901
HpaI GTT'AAC
Cuts at: 4491 4491
Size: 6901
KpnI G_GTAC'C
Cuts at: 4226 4226
Size: 6901
MfeI C'AATT_G
Cuts at: 4500 4500
Size: 6901
MluI A'CGCG_T
Cuts at: 6777 6777
Size: 6901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2815 3148 3579 6015 6199 1305
Size: 1510 333 431 2436 184 2007
Fragments arranged by size:
2436 2007 1510 431 333 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2943 3866 10
Size: 54 1425 343 1111 923 3045
Fragments arranged by size:
3045 1425 1111 923 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 3036 3267 3356 3947 4008 4158
Size: 648 1194 676 231 89 591 61 150
Cuts at: 4158 4859 5218 5377 518
Size: 701 359 159 2042
Fragments arranged by size:
2042 1194 701 676 648 591 359 231
159 150 89 61
MspA1I CmG'CkG
Cuts at: 1606 2072 3799 4317 4994 6142 6387 1606
Size: 466 1727 518 677 1148 245 2120
Fragments arranged by size:
2120 1727 1148 677 518 466 245
NaeI GCC'GGC
Cuts at: 3781 4094 3781
Size: 313 6588
NcoI C'CATG_G
Cuts at: 513 1065 4197 513
Size: 552 3132 3217
Fragments arranged by size:
3217 3132 552
NdeI CA'TA_TG
Cuts at: 387 2281 387
Size: 1894 5007
NgoMIV G'CCGG_C
Cuts at: 3779 4092 3779
Size: 313 6588
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6901
NotI GC'GGCC_GC
Cuts at: 4348 4348
Size: 6901
NspI r_CATG'y
Cuts at: 1132 1229 2485 3226 4157 4259 5804 1132
Size: 97 1256 741 931 102 1545 2229
Fragments arranged by size:
2229 1545 1256 931 741 102 97
PasI CC'CwG_GG
Cuts at: 1348 4098 1348
Size: 2750 4151
PciI A'CATG_T
Cuts at: 4153 5800 4153
Size: 1647 5254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2679 1108
Size: 1571 5330
PfoI T'CCnGG_A
Cuts at: 2018 2709 3066 4015 2018
Size: 691 357 949 4904
Fragments arranged by size:
4904 949 691 357
PmeI GTTT'AAAC
Cuts at: 4205 4205
Size: 6901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4970 5002 6483 6515 4970
Size: 32 1481 32 5356
Fragments arranged by size:
5356 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3694 3694
Size: 6901
PshAI GACnn'nnGTC
Cuts at: 1613 2271 1613
Size: 658 6243
PsiI TTA'TAA
Cuts at: 4471 4471
Size: 6901
PstI C_TGCA'G
Cuts at: 830 4253 830
Size: 3423 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6901
PvuII CAG'CTG
Cuts at: 3799 3799
Size: 6901
SacI G_AGCT'C
Cuts at: 2921 2921
Size: 6901
SalI G'TCGA_C
Cuts at: 1936 4243 1936
Size: 2307 4594
SanDI GG'GwC_CC
Cuts at: 3694 3694
Size: 6901
SapI GCTCTTCn'nnn_
Cuts at: 2035 2035
Size: 6901
SbfI CC_TGCA'GG
Cuts at: 4253 4253
Size: 6901
ScaI AGT'ACT
Cuts at: 5149 6861 5149
Size: 1712 5189
SfcI C'TryA_G
Cuts at: 826 1045 2367 4249 5384 6065 6256 826
Size: 219 1322 1882 1135 681 191 1471
Fragments arranged by size:
1882 1471 1322 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6901
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6901
SmaI CCC'GGG
Cuts at: 1872 2117 4228 1872
Size: 245 2111 4545
Fragments arranged by size:
4545 2111 245
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5005 5906 6168 820
Size: 197 926 3062 901 262 1553
Fragments arranged by size:
3062 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4692 493
Size: 4199 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6901
SphI G_CATG'C
Cuts at: 1132 4259 1132
Size: 3127 3774
SspI AAT'ATT
Cuts at: 5 52 4825 5
Size: 47 4773 2081
Fragments arranged by size:
4773 2081 47
StuI AGG'CCT
Cuts at: 1517 3154 1517
Size: 1637 5264
StyI C'CwwG_G
Cuts at: 513 1065 2564 2989 3102 4197 4340 513
Size: 552 1499 425 113 1095 143 3074
Fragments arranged by size:
3074 1499 1095 552 425 143 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2068 3395 4928 4945 5098 5283 5728
Size: 1255 488 1327 1533 17 153 185 445
Cuts at: 5728 325
Size: 1498
Fragments arranged by size:
1533 1498 1327 1255 488 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3786 5147 6859
Size: 274 80 33 51 1443 1809 1361 1712
Cuts at: 6859 96
Size: 138
Fragments arranged by size:
1809 1712 1443 1361 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 4073 4105 6861 6893 3098
Size: 32 943 32 2756 32 3106
Fragments arranged by size:
3106 2756 943 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6901
XbaI T'CTAG_A
Cuts at: 4237 4237
Size: 6901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2464 3052 3562 3859 2464
Size: 588 510 297 5506
Fragments arranged by size:
5506 588 510 297
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6901
XmaI C'CCGG_G
Cuts at: 1870 2115 4226 1870
Size: 245 2111 4545
Fragments arranged by size:
4545 2111 245
XmnI GAAnn'nnTTC
Cuts at: 4043 5030 4043
Size: 987 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4709 276
Size: 53 83 186 936 3175 2468
Fragments arranged by size:
3175 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI