Restriction map for
pFN21AE1564
(Circular) (Six-base) MAPSORT of: pFN21AE1564.seq Check: 2162 from: 1 to: 6733
pFN21AE1564 6733 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4543 278
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3279 3352 3764 4076 1937
Size: 1342 73 412 312 4594
Fragments arranged by size:
4594 1342 412 312 73
Acc65I G'GTAC_C
Cuts at: 4054 4054
Size: 6733
AclI AA'CG_TT
Cuts at: 1563 4860 5233 1563
Size: 3297 373 3063
Fragments arranged by size:
3297 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4796 6180 6448 1632
Size: 3164 1384 268 1917
Fragments arranged by size:
3164 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3092 820
Size: 197 2075 4461
Fragments arranged by size:
4461 2075 197
AflIII A'CryG_T
Cuts at: 1203 2754 5632 6609 1203
Size: 1551 2878 977 1327
Fragments arranged by size:
2878 1551 1327 977
AgeI A'CCGG_T
Cuts at: 5564 5564
Size: 6733
AhdI GACnn_n'nnGTC
Cuts at: 5462 5462
Size: 6733
AleI CACnn'nnGTG
Cuts at: 1166 2648 3896 1166
Size: 1482 1248 4003
Fragments arranged by size:
4003 1482 1248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6733
AlwNI CAG_nnn'CTG
Cuts at: 4476 6048 4476
Size: 1572 5161
ApaLI G'TGCA_C
Cuts at: 4790 5946 4790
Size: 1156 5577
ApoI r'AATT_y
Cuts at: 1493 2131 2468 2523 2945 2981 3364 3731 4027
Size: 638 337 55 422 36 383 367 296
Cuts at: 4027 4042 4268 4420 6529 1493
Size: 15 226 152 2109 1697
Fragments arranged by size:
2109 1697 638 422 383 367 337 296
226 152 55 36 15
AseI AT'TA_AT
Cuts at: 160 1032 3138 3936 5287 160
Size: 872 2106 798 1351 1606
Fragments arranged by size:
2106 1606 1351 872 798
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
AvaI C'yCGr_G
Cuts at: 1870 1943 4058 1870
Size: 73 2115 4545
Fragments arranged by size:
4545 2115 73
BamHI G'GATC_C
Cuts at: 4063 4430 4063
Size: 367 6366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4054 5509 618
Size: 325 217 627 2267 1455 1842
Fragments arranged by size:
2267 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3452 3994 4052 1391
Size: 2061 542 58 4072
Fragments arranged by size:
4072 2061 542 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2261 3804 928
Size: 971 362 1543 3857
Fragments arranged by size:
3857 1543 971 362
BbvCI CC'TCA_GC
Cuts at: 3513 3513
Size: 6733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4924 4958 1903
Size: 34 2987 34 3678
Fragments arranged by size:
3678 2987 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3514 3681 4625 5835 3514
Size: 167 944 1210 4412
Fragments arranged by size:
4412 1210 944 167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6733
BfrBI ATG'CAT
Cuts at: 2178 2178
Size: 6733
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2694 5344 136
Size: 107 122 71 631 605 1022 2650 1525
Fragments arranged by size:
2650 1525 1022 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6728 6728
Size: 6733
BlpI GC'TnA_GC
Cuts at: 2763 4150 2763
Size: 1387 5346
Bme1580I G_kGCm'C
Cuts at: 1790 3563 3606 4794 5950 1790
Size: 1773 43 1188 1156 2573
Fragments arranged by size:
2573 1773 1188 1156 43
BmgBI CAC'GTC
Cuts at: 1318 2306 1318
Size: 988 5745
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2295 4464 5422
Size: 416 53 83 399 24 878 2169 958
Cuts at: 5422 442
Size: 1753
Fragments arranged by size:
2169 1753 958 878 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6733
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3466 3466
Size: 6733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2805 3144 3776 5393 1239
Size: 1566 339 632 1617 2579
Fragments arranged by size:
2579 1617 1566 632 339
Bpu10I CC'TnA_GC
Cuts at: 3513 3513
Size: 6733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4858 5723 6021 4858
Size: 865 298 5570
Fragments arranged by size:
5570 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5396 882
Size: 617 3897 2219
Fragments arranged by size:
3897 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4524 493
Size: 711 3320 2702
Fragments arranged by size:
3320 2702 711
BsaBI GATnn'nnATC
Cuts at: 6727 6727
Size: 6733
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4540 4922 5557 275
Size: 53 83 186 936 3007 382 635 1451
Fragments arranged by size:
3007 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2991 5166 5564 5838 5985 6396 2991
Size: 2175 398 274 147 411 3328
Fragments arranged by size:
3328 2175 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3117 3147 1178
Size: 30 165 30 1714 30 4764
Fragments arranged by size:
4764 1714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 1186
Size: 853 5880
BseYI C'CCAG_C
Cuts at: 5936 6432 5936
Size: 496 6237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2286 1163
Size: 573 550 5610
Fragments arranged by size:
5610 573 550
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4183 4944 5972 1540
Size: 275 186 2182 761 1028 2301
Fragments arranged by size:
2301 2182 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2764 3994 4794 4879 5950 2764
Size: 1230 800 85 1071 3547
Fragments arranged by size:
3547 1230 1071 800 85
BsmI GAATG_Cn'
Cuts at: 2180 2489 2621 2690 2747 3907 4244 4337 2180
Size: 309 132 69 57 1160 337 93 4576
Fragments arranged by size:
4576 1160 337 309 132 93 69 57
BsmBI CGTCTCn'nnnn_
Cuts at: 2071 2071
Size: 6733
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2764 3452 3563 3606 3994 4052 4794
Size: 399 974 688 111 43 388 58 742
Cuts at: 4794 4879 5950 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 974 742 688 399 388 111
85 58 43
BspEI T'CCGG_A
Cuts at: 2991 6396 2991
Size: 3405 3328
BspHI T'CATG_A
Cuts at: 4622 6352 4622
Size: 1730 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4088 844
Size: 3244 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4620 1394
Size: 429 2797 3507
Fragments arranged by size:
3507 2797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2389 3424 3840 5228 5402 65
Size: 1183 1141 1035 416 1388 174 1396
Fragments arranged by size:
1396 1388 1183 1141 1035 416 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5377 5564 1700
Size: 110 3567 187 2869
Fragments arranged by size:
3567 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3943 96
Size: 3847 2886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6733
BssSI C'ACGA_G
Cuts at: 4793 5805 4793
Size: 1012 5721
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6733
BstBI TT'CG_AA
Cuts at: 5537 5537
Size: 6733
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6733
BstXI CCAn_nnnn'nTGG
Cuts at: 3859 4011 3859
Size: 152 6581
BstYI r'GATC_y
Cuts at: 1972 2491 3952 4063 4430 4815 4832 6284 6370
Size: 519 1461 111 367 385 17 1452 86
Cuts at: 6370 6382 6728 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1461 1452 519 385 367 346 111
86 17 12
BstZ17I GTA'TAC
Cuts at: 3280 3353 3280
Size: 73 6660
BtgI C'CryG_G
Cuts at: 513 1065 3555 513
Size: 552 2490 3691
Fragments arranged by size:
3691 2490 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5994
Fragments arranged by size:
5994 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 4249 5043 5063 2295
Size: 1954 794 20 3965
Fragments arranged by size:
3965 1954 794 20
ClaI AT'CG_AT
Cuts at: 1554 3036 1554
Size: 1482 5251
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6698
DraI TTT'AAA
Cuts at: 2943 4037 2943
Size: 1094 5639
DraIII CAC_nnn'GTG
Cuts at: 3068 3068
Size: 6733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5740 809
Size: 507 4424 1802
Fragments arranged by size:
4424 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3849 4180 5069
Size: 54 1425 50 275 18 2019 331 889
Cuts at: 5069 6698 8
Size: 1629 43
Fragments arranged by size:
2019 1629 1425 889 331 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4180 1537
Size: 275 2368 4090
Fragments arranged by size:
4090 2368 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 4663 1353
Size: 1484 1826 3423
Fragments arranged by size:
3423 1826 1484
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2927 5320 5694 5840 387
Size: 1064 138 1338 2393 374 146 1280
Fragments arranged by size:
2393 1338 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3992 3992
Size: 6733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2805 3144 3776 4796 5393 6180 6448
Size: 393 1173 339 632 1020 597 787 268
Cuts at: 6448 1239
Size: 1524
Fragments arranged by size:
1524 1173 1020 787 632 597 393 339
268
EcoO109I rG'GnC_Cy
Cuts at: 2011 3694 2011
Size: 1683 5050
EcoRI G'AATT_C
Cuts at: 4027 4042 4420 4027
Size: 15 378 6340
Fragments arranged by size:
6340 378 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3107 3107
Size: 6733
FspI TGC'GCA
Cuts at: 5239 5239
Size: 6733
HaeII r_GCGC'y
Cuts at: 1678 2026 2036 5880 1678
Size: 348 10 3844 2531
Fragments arranged by size:
3844 2531 348 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2163 2195 2603 2635 2694
Size: 32 135 32 591 32 408 32 59
Cuts at: 2694 2726 2729 2761 3319 3351 3617 3649 3704
Size: 32 3 32 558 32 266 32 55
Cuts at: 3704 3736 5374 5406 5448 5480 1373
Size: 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 591 558 408 266 135 59
55 42 32 32 32 32 32 32
32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2313 3742 3765 4077 4323 669
Size: 988 281 375 1429 23 312 246 3079
Fragments arranged by size:
3079 1429 988 375 312 281 246 23
HindIII A'AGCT_T
Cuts at: 748 2418 748
Size: 1670 5063
HpaI GTT'AAC
Cuts at: 4323 4323
Size: 6733
KpnI G_GTAC'C
Cuts at: 4058 4058
Size: 6733
MfeI C'AATT_G
Cuts at: 4332 4332
Size: 6733
MluI A'CGCG_T
Cuts at: 6609 6609
Size: 6733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3930 5847 6031 1305
Size: 2625 1917 184 2007
Fragments arranged by size:
2625 2007 1917 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3851 10
Size: 54 1425 343 2019 2892
Fragments arranged by size:
2892 2019 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 3528 3896 3926 4691 5050 5209
Size: 648 1482 880 368 30 765 359 159
Cuts at: 5209 518
Size: 2042
Fragments arranged by size:
2042 1482 880 765 648 368 359 159
30
MspA1I CmG'CkG
Cuts at: 1606 2648 4149 4826 5974 6219 1606
Size: 1042 1501 677 1148 245 2120
Fragments arranged by size:
2120 1501 1148 1042 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6181
NdeI CA'TA_TG
Cuts at: 387 2998 387
Size: 2611 4122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6733
NotI GC'GGCC_GC
Cuts at: 4180 4180
Size: 6733
NsiI A_TGCA'T
Cuts at: 2180 2180
Size: 6733
NspI r_CATG'y
Cuts at: 1132 1229 2758 3527 4091 5636 1132
Size: 97 1529 769 564 1545 2229
Fragments arranged by size:
2229 1545 1529 769 564 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6733
PciI A'CATG_T
Cuts at: 2754 5632 2754
Size: 2878 3855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6733
PmeI GTTT'AAAC
Cuts at: 4037 4037
Size: 6733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4802 4834 6315 6347 4802
Size: 32 1481 32 5188
Fragments arranged by size:
5188 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2011
Size: 6733
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6733
PsiI TTA'TAA
Cuts at: 3495 3912 4303 3495
Size: 417 391 5925
Fragments arranged by size:
5925 417 391
PstI C_TGCA'G
Cuts at: 830 4085 830
Size: 3255 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6733
PvuII CAG'CTG
Cuts at: 2648 2648
Size: 6733
SacI G_AGCT'C
Cuts at: 3994 3994
Size: 6733
SalI G'TCGA_C
Cuts at: 1936 3763 4075 1936
Size: 1827 312 4594
Fragments arranged by size:
4594 1827 312
SbfI CC_TGCA'GG
Cuts at: 4085 4085
Size: 6733
ScaI AGT'ACT
Cuts at: 4981 6693 4981
Size: 1712 5021
SexAI A'CCwGG_T
Cuts at: 2443 2443
Size: 6733
SfcI C'TryA_G
Cuts at: 826 1045 2698 4081 5216 5897 6088 826
Size: 219 1653 1383 1135 681 191 1471
Fragments arranged by size:
1653 1471 1383 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6733
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6733
SmaI CCC'GGG
Cuts at: 1872 4060 1872
Size: 2188 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3092 4837 5738 6000 820
Size: 197 926 1149 1745 901 262 1553
Fragments arranged by size:
1745 1553 1149 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4524 493
Size: 4031 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6733
SphI G_CATG'C
Cuts at: 1132 4091 1132
Size: 2959 3774
SspI AAT'ATT
Cuts at: 5 52 3544 3934 4657 5
Size: 47 3492 390 723 2081
Fragments arranged by size:
3492 2081 723 390 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6733
StyI C'CwwG_G
Cuts at: 513 1065 3579 3618 4172 513
Size: 552 2514 39 554 3074
Fragments arranged by size:
3074 2514 554 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2006 3851 4760 4777 4930 5115 5560
Size: 1255 426 1845 909 17 153 185 445
Cuts at: 5560 325
Size: 1498
Fragments arranged by size:
1845 1498 1255 909 445 426 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3943 4979 6691
Size: 274 80 33 51 1443 1966 1036 1712
Cuts at: 6691 96
Size: 138
Fragments arranged by size:
1966 1712 1443 1036 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2110 2142 6693 6725 2110
Size: 32 4551 32 2118
Fragments arranged by size:
4551 2118 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6733
XbaI T'CTAG_A
Cuts at: 3340 4069 3340
Size: 729 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6733
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6733
XmaI C'CCGG_G
Cuts at: 1870 4058 1870
Size: 2188 4545
XmnI GAAnn'nnTTC
Cuts at: 2419 2819 3402 3801 4862 2419
Size: 400 583 399 1061 4290
Fragments arranged by size:
4290 1061 583 400 399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4541 276
Size: 53 83 186 936 3007 2468
Fragments arranged by size:
3007 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI