Restriction map for
pFN21AE1563
(Circular) (Six-base) MAPSORT of: pFN21AE1563.seq Check: 4612 from: 1 to: 6079
pFN21AE1563 6079 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2559 2559
Size: 6079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 2775 3422 1937
Size: 211 627 647 4594
Fragments arranged by size:
4594 647 627 211
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 3230 4206 4579 1563
Size: 1667 976 373 3063
Fragments arranged by size:
3063 1667 976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3096 4142 5526 5794 1632
Size: 1464 1046 1384 268 1917
Fragments arranged by size:
1917 1464 1384 1046 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 4978 5955 1203
Size: 3775 977 1327
Fragments arranged by size:
3775 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 3213 4808 3213
Size: 1595 4484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2894 1184
Size: 1710 4369
AlwNI CAG_nnn'CTG
Cuts at: 2764 3007 3179 3203 3822 5394 2764
Size: 243 172 24 619 1572 3449
Fragments arranged by size:
3449 1572 619 243 172 24
ApaI G_GGCC'C
Cuts at: 2429 2429
Size: 6079
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 3388 3614 3766 5875 1493
Size: 1895 226 152 2109 1697
Fragments arranged by size:
2109 1895 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 2595 3404 1870
Size: 73 645 7 809 4545
Fragments arranged by size:
4545 809 645 73 7
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 4855 618
Size: 325 217 627 1613 1455 1842
Fragments arranged by size:
1842 1613 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 2429 3289 3398 1391
Size: 835 203 860 109 4072
Fragments arranged by size:
4072 860 835 203 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2017 928
Size: 971 118 4990
Fragments arranged by size:
4990 971 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2396 2430 4270 4304 1903
Size: 34 459 34 1840 34 3678
Fragments arranged by size:
3678 1840 459 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 136
Size: 107 122 71 631 605 3018 1525
Fragments arranged by size:
3018 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 2429 4140 5296 1790
Size: 639 1711 1156 2573
Fragments arranged by size:
2573 1711 1156 639
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3810 4768 442
Size: 416 53 83 399 24 2393 958 1753
Fragments arranged by size:
2393 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3303 3303
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 4739 1239
Size: 974 2526 2579
Fragments arranged by size:
2579 2526 974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3249 4204 5069 5367 3249
Size: 955 865 298 3961
Fragments arranged by size:
3961 955 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2585 4742 882
Size: 617 1086 2157 2219
Fragments arranged by size:
2219 2157 1086 617
BsaAI yAC'GTr
Cuts at: 493 1204 2188 3870 493
Size: 711 984 1682 2702
Fragments arranged by size:
2702 1682 984 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2325 2607 4512 4910 5184 5331 5742 2325
Size: 282 1905 398 274 147 411 2662
Fragments arranged by size:
2662 1905 411 398 282 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2297 2327 2646 2676 1178
Size: 30 165 30 894 30 319 30 4581
Fragments arranged by size:
4581 894 319 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2333 2972 3295 1186
Size: 841 306 639 323 3970
Fragments arranged by size:
3970 841 639 323 306
BseYI C'CCAG_C
Cuts at: 2977 3290 5282 5778 2977
Size: 313 1992 496 3278
Fragments arranged by size:
3278 1992 496 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3341 1163
Size: 573 1605 3901
Fragments arranged by size:
3901 1605 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3529 4290 5318 1540
Size: 275 186 1528 761 1028 2301
Fragments arranged by size:
2301 1528 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2700 3289 4140 4225 5296 2700
Size: 589 851 85 1071 3483
Fragments arranged by size:
3483 1071 851 589 85
BsmI GAATG_Cn'
Cuts at: 2132 2370 2883 3590 3683 2132
Size: 238 513 707 93 4528
Fragments arranged by size:
4528 707 513 238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2429 2700 3289 3398 4140 4225
Size: 399 436 203 271 589 109 742 85
Cuts at: 4225 5296 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 589 436 399 271 203
109 85
BspEI T'CCGG_A
Cuts at: 2325 5742 2325
Size: 3417 2662
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2022 2559 3434 844
Size: 1178 537 875 3489
Fragments arranged by size:
3489 1178 875 537
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4723 4910 1700
Size: 110 2913 187 2869
Fragments arranged by size:
2913 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2109 96
Size: 2013 4066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 2219 4139 5151 2219
Size: 1920 1012 3147
Fragments arranged by size:
3147 1920 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 2065 3116 1184
Size: 881 1051 4147
Fragments arranged by size:
4147 1051 881
BstXI CCAn_nnnn'nTGG
Cuts at: 2568 2568
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074
Size: 1437 367 385 17 1452 86 12 346
Cuts at: 6074 1972
Size: 1977
Fragments arranged by size:
1977 1452 1437 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2650 3121 3210 513
Size: 552 1585 471 89 3382
Fragments arranged by size:
3382 1585 552 471 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 3595 4389 4409 3595
Size: 794 20 5265
Fragments arranged by size:
5265 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2454 2489 529
Size: 35 1890 35 4119
Fragments arranged by size:
4119 1890 35 35
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 2766 3526
Size: 54 1425 50 275 18 837 99 760
Cuts at: 3526 4415 6044 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 837 760 275 99 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2759 2949 4009 1353
Size: 698 708 190 1060 3423
Fragments arranged by size:
3423 1060 708 698 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2705 4666 5040 5186 387
Size: 1064 138 1116 1961 374 146 1280
Fragments arranged by size:
1961 1280 1116 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3287 3287
Size: 6079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 3096 4142 4739 5526 5794 1239
Size: 393 581 883 1046 597 787 268 1524
Fragments arranged by size:
1524 1046 883 787 597 581 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3011 3011
Size: 6079
EcoO109I rG'GnC_Cy
Cuts at: 2425 2537 3215 3371 2425
Size: 112 678 156 5133
Fragments arranged by size:
5133 678 156 112
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 3199 3231 4720
Size: 32 135 32 431 32 1164 32 1489
Cuts at: 4720 4752 4794 4826 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1489 1164 431 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3238 3423 3669 669
Size: 988 281 1300 185 246 3079
Fragments arranged by size:
3079 1300 988 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3238 3669 3238
Size: 431 5648
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2283 2789 5193 5377 1305
Size: 978 506 2404 184 2007
Fragments arranged by size:
2404 2007 978 506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 2768 10
Size: 54 1425 343 837 99 3321
Fragments arranged by size:
3321 1425 837 343 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 2791 4037 4396 4555 518
Size: 648 919 706 1246 359 159 2042
Fragments arranged by size:
2042 1246 919 706 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2968 3361 3495 4172 5320 5565 1606
Size: 693 669 393 134 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 669 393 245 134
NcoI C'CATG_G
Cuts at: 513 1065 3121 513
Size: 552 2056 3471
Fragments arranged by size:
3471 2056 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 2260 3437 4982 1132
Size: 97 1031 1177 1545 2229
Fragments arranged by size:
2229 1545 1177 1031 97
PasI CC'CwG_GG
Cuts at: 1348 2422 1348
Size: 1074 5005
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2764 3007 1108
Size: 1656 243 4180
Fragments arranged by size:
4180 1656 243
PfoI T'CCnGG_A
Cuts at: 2358 3006 2358
Size: 648 5431
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3215 3215
Size: 6079
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 2425 2425
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2298 3366 3431 830
Size: 1468 1068 65 3478
Fragments arranged by size:
3478 1468 1068 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2299 3361 2299
Size: 1062 5017
SacI G_AGCT'C
Cuts at: 3289 3289
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SapI GCTCTTCn'nnn_
Cuts at: 2759 2759
Size: 6079
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 2149 2294 3362 3427 4562 5243 5434
Size: 219 1104 145 1068 65 1135 681 191
Cuts at: 5434 826
Size: 1471
Fragments arranged by size:
1471 1135 1104 1068 681 219 191 145
65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 2597 3406 1872
Size: 725 809 4545
Fragments arranged by size:
4545 809 725
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3264 4183 5084 5346 820
Size: 197 926 1321 919 901 262 1553
Fragments arranged by size:
1553 1321 926 919 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2226 2769 2803 3121 3518 513
Size: 552 1161 543 34 318 397 3074
Fragments arranged by size:
3074 1161 552 543 397 318 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 4325 6037
Size: 274 80 33 51 1443 132 2216 1712
Cuts at: 6037 96
Size: 138
Fragments arranged by size:
2216 1712 1443 274 138 132 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2297 2329 2646 2678 6039 6071 2297
Size: 32 317 32 3361 32 2305
Fragments arranged by size:
3361 2305 317 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 2595 3404 1870
Size: 725 809 4545
Fragments arranged by size:
4545 809 725
XmnI GAAnn'nnTTC
Cuts at: 3015 4208 3015
Size: 1193 4886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI