Restriction map for
pFN21AE1562
(Circular) (Six-base) MAPSORT of: pFN21AE1562.seq Check: 3852 from: 1 to: 6151
pFN21AE1562 6151 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2345 2511 2345
Size: 166 5985
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2801 4214 5598 5866 1632
Size: 1169 1413 1384 268 1917
Fragments arranged by size:
1917 1413 1384 1169 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 2153 3201 1166
Size: 987 1048 4116
Fragments arranged by size:
4116 1048 987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 2244 3022 3040 3131 3151 3894 5466 2244
Size: 778 18 91 20 743 1572 2929
Fragments arranged by size:
2929 1572 778 743 91 20 18
ApaI G_GGCC'C
Cuts at: 2519 2519
Size: 6151
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 3311 3460 3686 3838 5947 1493
Size: 1818 149 226 152 2109 1697
Fragments arranged by size:
2109 1818 1697 226 152 149
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 2914 3476 1870
Size: 73 828 143 562 4545
Fragments arranged by size:
4545 828 562 143 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 3472 4927 618
Size: 325 217 627 516 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 516 325 217
BanII G_rGCy'C
Cuts at: 1391 2519 2970 3470 1391
Size: 1128 451 500 4072
Fragments arranged by size:
4072 1128 500 451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3390 928
Size: 971 1491 3689
Fragments arranged by size:
3689 1491 971
BbvCI CC'TCA_GC
Cuts at: 2928 2928
Size: 6151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4342 4376 1903
Size: 34 50 34 2321 34 3678
Fragments arranged by size:
3678 2321 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 2964 3568 2964
Size: 604 5547
Bme1580I G_kGCm'C
Cuts at: 1790 2519 4212 5368 1790
Size: 729 1693 1156 2573
Fragments arranged by size:
2573 1693 1156 729
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3882 4840 442
Size: 416 53 83 399 24 2465 958 1753
Fragments arranged by size:
2465 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2438 1056
Size: 1382 4769
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2852 4811 1239
Size: 1613 1959 2579
Fragments arranged by size:
2579 1959 1613
Bpu10I CC'TnA_GC
Cuts at: 2928 3015 2928
Size: 87 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 4276 5141 5439 2147
Size: 2129 865 298 2859
Fragments arranged by size:
2859 2129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4814 882
Size: 617 3315 2219
Fragments arranged by size:
3315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5926
Fragments arranged by size:
5926 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6151
BseYI C'CCAG_C
Cuts at: 2751 3233 5354 5850 2751
Size: 482 2121 496 3052
Fragments arranged by size:
3052 2121 496 482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2230 1163
Size: 573 494 5084
Fragments arranged by size:
5084 573 494
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2970 4212 4297 5368 2970
Size: 1242 85 1071 3753
Fragments arranged by size:
3753 1242 1071 85
BsmI GAATG_Cn'
Cuts at: 3662 3755 3662
Size: 93 6058
BsmBI CGTCTCn'nnnn_
Cuts at: 2019 3434 2019
Size: 1415 4736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2519 2970 3470 4212 4297 5368 1391
Size: 399 729 451 500 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 500 451 399 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2345 2511 3159 3506 844
Size: 1501 166 648 347 3489
Fragments arranged by size:
3489 1501 648 347 166
BsrBI CCG'CTC
Cuts at: 1394 1823 2211 3246 4038 1394
Size: 429 388 1035 792 3507
Fragments arranged by size:
3507 1035 792 429 388
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 3007 4646 4820 65
Size: 1183 1044 715 1639 174 1396
Fragments arranged by size:
1639 1396 1183 1044 715 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2607 4795 4982 1700
Size: 110 797 2188 187 2869
Fragments arranged by size:
2869 2188 797 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3151 794
Size: 2357 3794
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 2467
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 2068 2685 3481 3848 4233 4250 5702 5788
Size: 96 617 796 367 385 17 1452 86
Cuts at: 5788 5800 6146 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 796 617 385 367 346 96
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2064 2064
Size: 6151
BtgI C'CryG_G
Cuts at: 513 1065 2907 513
Size: 552 1842 3757
Fragments arranged by size:
3757 1842 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 3247 487
Size: 683 56 899 1122 3391
Fragments arranged by size:
3391 1122 899 683 56
BtsI GCAGTG_nn'
Cuts at: 2239 2313 3223 3667 4461 4481 2239
Size: 74 910 444 794 20 3909
Fragments arranged by size:
3909 910 794 444 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2134 2169 2382 2417 529
Size: 35 1570 35 213 35 4263
Fragments arranged by size:
4263 1570 213 35 35 35
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116
Size: 54 1425 50 275 18 1768 889 1629
Cuts at: 6116 8
Size: 43
Fragments arranged by size:
1768 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2777 3162 4081 1353
Size: 1424 385 919 3423
Fragments arranged by size:
3423 1424 919 385
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2626 3238 4738 5112 5258 387
Size: 1064 138 1037 612 1500 374 146 1280
Fragments arranged by size:
1500 1280 1064 1037 612 374 146 138
EcoICRI GAG'CTC
Cuts at: 2968 2968
Size: 6151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2801 2852 4214 4811 5598 5866 1239
Size: 393 1169 51 1362 597 787 268 1524
Fragments arranged by size:
1524 1362 1169 787 597 393 268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2415 2415
Size: 6151
EcoO109I rG'GnC_Cy
Cuts at: 2421 2515 2516 2538 3019 2421
Size: 94 1 22 481 5553
Fragments arranged by size:
5553 481 94 22 1
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2837 2704
Size: 133 6018
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 5298 1678
Size: 3620 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3321 3353 4792 4824 4866
Size: 32 135 32 1749 32 1439 32 42
Cuts at: 4866 4898 1373
Size: 32 2626
Fragments arranged by size:
2626 1749 1439 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2359 3495 3741 669
Size: 988 281 421 1136 246 3079
Fragments arranged by size:
3079 1136 988 421 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2083 5265 5449 1305
Size: 778 3182 184 2007
Fragments arranged by size:
3182 2007 778 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 3201 3287 4109 4468 4627 518
Size: 648 987 1048 86 822 359 159 2042
Fragments arranged by size:
2042 1048 987 822 648 359 159 86
MspA1I CmG'CkG
Cuts at: 1606 2199 2241 2300 2909 3037 3567 4244 5392
Size: 593 42 59 609 128 530 677 1148
Cuts at: 5392 5637 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 609 593 530 245 128
59 42
NaeI GCC'GGC
Cuts at: 2609 2609
Size: 6151
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5599
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NgoMIV G'CCGG_C
Cuts at: 2607 2607
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 2434 1052
Size: 1382 4769
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 3509 5054 1132
Size: 97 2280 1545 2229
Fragments arranged by size:
2280 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2396 1108
Size: 1288 4863
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2421 3019 2421
Size: 598 5553
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3309 3721 3309
Size: 412 5739
PspOMI G'GGCC_C
Cuts at: 2515 2515
Size: 6151
PspXI vC'TCGA_Gb
Cuts at: 2771 2914 2771
Size: 143 6008
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 6119
PstI C_TGCA'G
Cuts at: 830 2537 2622 3503 830
Size: 1707 85 881 3478
Fragments arranged by size:
3478 1707 881 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2241 2241
Size: 6151
SacI G_AGCT'C
Cuts at: 2970 2970
Size: 6151
SacII CC_GC'GG
Cuts at: 2910 2910
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SanDI GG'GwC_CC
Cuts at: 2421 2421
Size: 6151
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 3161 4399 6111 3161
Size: 1238 1712 3201
Fragments arranged by size:
3201 1712 1238
SfcI C'TryA_G
Cuts at: 826 1045 2533 2618 2656 3499 4634 5315 5506
Size: 219 1488 85 38 843 1135 681 191
Cuts at: 5506 826
Size: 1471
Fragments arranged by size:
1488 1471 1135 843 681 219 191 85
38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2771 2914 4255 5156 5418
Size: 197 926 219 609 143 1341 901 262
Cuts at: 5418 820
Size: 1553
Fragments arranged by size:
1553 1341 926 901 609 262 219 197
143
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 3102 1517
Size: 1585 4566
StyI C'CwwG_G
Cuts at: 513 1065 2098 2569 2995 3590 513
Size: 552 1033 471 426 595 3074
Fragments arranged by size:
3074 1033 595 552 471 426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4178 4195 4348 4533 4978 325
Size: 1255 2598 17 153 185 445 1498
Fragments arranged by size:
2598 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 3159 4397
Size: 274 80 33 51 1443 573 609 1238
Cuts at: 4397 6109 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1238 609 573 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3061 3093 3396 3428 6111 6143 3061
Size: 32 303 32 2683 32 3069
Fragments arranged by size:
3069 2683 303 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6151
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3230 3230
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 2771 2914 1943
Size: 828 143 5180
Fragments arranged by size:
5180 828 143
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI RsrII SapI SexAI
SfiI SfoI SrfI SwaI