Restriction map for
pFN21AE1560
(Circular) (Six-base) MAPSORT of: pFN21AE1560.seq Check: 6889 from: 1 to: 5443
pFN21AE1560 5443 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2688 2786 1937
Size: 751 98 4594
Fragments arranged by size:
4594 751 98
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3506 4890 5158 1632
Size: 1874 1384 268 1917
Fragments arranged by size:
1917 1874 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 2591 4274 2591
Size: 1683 3760
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 2372 1166
Size: 1206 4237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 2514
Size: 32 5411
AlwNI CAG_nnn'CTG
Cuts at: 2125 3186 4758 2125
Size: 1061 1572 2810
Fragments arranged by size:
2810 1572 1061
ApaI G_GGCC'C
Cuts at: 2233 2619 2233
Size: 386 5057
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2752 2978 3130 5239 1493
Size: 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 1870
Size: 73 825 4545
Fragments arranged by size:
4545 825 73
BamHI G'GATC_C
Cuts at: 2572 2773 3140 2572
Size: 201 367 4875
Fragments arranged by size:
4875 367 201
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2764 4219 618
Size: 325 217 627 977 1455 1842
Fragments arranged by size:
1842 1455 977 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2233 2619 2762 1391
Size: 842 386 143 4072
Fragments arranged by size:
4072 842 386 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2588 928
Size: 971 689 3783
Fragments arranged by size:
3783 971 689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3634 3668 1903
Size: 34 50 34 1613 34 3678
Fragments arranged by size:
3678 1613 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2386 4054 136
Size: 107 122 71 631 605 714 1668 1525
Fragments arranged by size:
1668 1525 714 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2619 3504 4660 1790
Size: 443 386 885 1156 2573
Fragments arranged by size:
2573 1156 885 443 386
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2664 4103 1239
Size: 1127 298 1439 2579
Fragments arranged by size:
2579 1439 1127 298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2704 4106 882
Size: 617 1205 1402 2219
Fragments arranged by size:
2219 1402 1205 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3250 3632 4267 275
Size: 53 83 186 936 1717 382 635 1451
Fragments arranged by size:
1717 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2591 3876 4274 4548 4695 5106 2591
Size: 1285 398 274 147 411 2928
Fragments arranged by size:
2928 1285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2009 2075 1186
Size: 823 66 4554
Fragments arranged by size:
4554 823 66
BseYI C'CCAG_C
Cuts at: 2051 2259 2442 4646 5142 2051
Size: 208 183 2204 496 2352
Fragments arranged by size:
2352 2204 496 208 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2449 2522 1163
Size: 573 713 73 4084
Fragments arranged by size:
4084 713 573 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2465 3504 3589 4660 2465
Size: 1039 85 1071 3248
Fragments arranged by size:
3248 1071 1039 85
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
BsmBI CGTCTCn'nnnn_
Cuts at: 2531 2531
Size: 5443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2465 2619 2762 3504 3589 4660
Size: 399 443 232 154 143 742 85 1071
Cuts at: 4660 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 443 399 232 154 143
85
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 844
Size: 1954 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2254 3330 1394
Size: 429 431 1076 3507
Fragments arranged by size:
3507 1076 431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2591 4087 4274 1700
Size: 110 781 1496 187 2869
Fragments arranged by size:
2869 1496 781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 2621 3503 4515 2621
Size: 882 1012 3549
Fragments arranged by size:
3549 1012 882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2572 2773 3140 3525 3542 4994 5080 5092
Size: 600 201 367 385 17 1452 86 12
Cuts at: 5092 5438 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 600 385 367 346 201 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2533 513
Size: 552 1468 3423
Fragments arranged by size:
3423 1468 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2194 2437 2477 2959 3753 3773 2194
Size: 243 40 482 794 20 3864
Fragments arranged by size:
3864 794 482 243 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2890 3779 5408
Size: 54 1425 50 275 18 1060 889 1629
Cuts at: 5408 8
Size: 43
Fragments arranged by size:
1629 1425 1060 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2138 2534 3373 1353
Size: 776 9 396 839 3423
Fragments arranged by size:
3423 839 776 396 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 4404 4550 387
Size: 1064 138 2441 374 146 1280
Fragments arranged by size:
2441 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2664 3506 4103 4890 5158 1239
Size: 393 734 298 842 597 787 268 1524
Fragments arranged by size:
1524 842 787 734 597 393 298 268
EcoO109I rG'GnC_Cy
Cuts at: 2229 2615 2616 2229
Size: 386 1 5056
Fragments arranged by size:
5056 386 1
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 2740 4590 1678
Size: 1062 1850 2531
Fragments arranged by size:
2531 1850 1062
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2176 2208 2676 2708 4084
Size: 32 135 32 604 32 468 32 1376
Cuts at: 4084 4116 4158 4190 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1376 604 468 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2120 2346 4557 4741 1305
Size: 815 226 2211 184 2007
Fragments arranged by size:
2211 2007 815 226 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3621
Fragments arranged by size:
3621 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2372 3401 3760 3919 518
Size: 648 996 210 1029 359 159 2042
Fragments arranged by size:
2042 1029 996 648 359 210 159
MspA1I CmG'CkG
Cuts at: 1606 2189 2344 2372 2425 2859 3536 4684 4929
Size: 583 155 28 53 434 677 1148 245
Cuts at: 4929 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 583 434 245 155 53
28
NcoI C'CATG_G
Cuts at: 513 1065 2533 513
Size: 552 1468 3423
Fragments arranged by size:
3423 1468 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2801 4346 1132
Size: 97 1572 1545 2229
Fragments arranged by size:
2229 1572 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2045 1348
Size: 697 4746
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5443
PfoI T'CCnGG_A
Cuts at: 2680 2680
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PspOMI G'GGCC_C
Cuts at: 2229 2615 2229
Size: 386 5057
PstI C_TGCA'G
Cuts at: 830 2343 2484 2795 830
Size: 1513 141 311 3478
Fragments arranged by size:
3478 1513 311 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
PvuII CAG'CTG
Cuts at: 2189 2344 2372 2425 2189
Size: 155 28 53 5207
Fragments arranged by size:
5207 155 53 28
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2339 2480 2791 3926 4607 4798 826
Size: 219 1294 141 311 1135 681 191 1471
Fragments arranged by size:
1471 1294 1135 681 311 219 191 141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2770 1872
Size: 898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2223 2533 2641 2882 513
Size: 552 1158 310 108 241 3074
Fragments arranged by size:
3074 1158 552 310 241 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 3487 3640 3825 4270 325
Size: 1255 1890 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3689 5401 96
Size: 274 80 33 51 1443 1712 1712 138
Fragments arranged by size:
1712 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 2501 2533 5403 5435 2134
Size: 32 335 32 2870 32 2142
Fragments arranged by size:
2870 2142 335 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2768 1870
Size: 898 4545
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI