Restriction map for
pFN21AE1556
(Circular) (Six-base) MAPSORT of: pFN21AE1556.seq Check: 4619 from: 1 to: 6085
pFN21AE1556 6085 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3895 278
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 1937
Size: 1491 4594
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2167 2261 4148 5532 5800 1632
Size: 503 32 94 1887 1384 268 1917
Fragments arranged by size:
1917 1887 1384 503 268 94 32
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 2996 4984 5961 1203
Size: 1793 1988 977 1327
Fragments arranged by size:
1988 1793 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 2531 1166
Size: 1365 4720
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AlwNI CAG_nnn'CTG
Cuts at: 2113 2332 2817 3137 3196 3828 5400 2113
Size: 219 485 320 59 632 1572 2798
Fragments arranged by size:
2798 1572 632 485 320 219 59
ApaLI G'TGCA_C
Cuts at: 2521 4142 5298 2521
Size: 1621 1156 3308
Fragments arranged by size:
3308 1621 1156
ApoI r'AATT_y
Cuts at: 1493 2722 2969 2977 3147 3178 3394 3620 3772
Size: 1229 247 8 170 31 216 226 152
Cuts at: 3772 5881 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1229 247 226 216 170 152
31 8
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2857 3410 1870
Size: 73 914 553 4545
Fragments arranged by size:
4545 914 553 73
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3406 4861 618
Size: 325 217 627 1619 1455 1842
Fragments arranged by size:
1842 1619 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3326 3404 1391
Size: 1935 78 4072
Fragments arranged by size:
4072 1935 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 2104 1548
Size: 556 5529
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2234 3124 4696
Size: 107 122 71 631 605 562 890 1572
Cuts at: 4696 136
Size: 1525
Fragments arranged by size:
1572 1525 890 631 605 562 122 107
71
BglII A'GATC_T
Cuts at: 2759 6080 2759
Size: 3321 2764
BlpI GC'TnA_GC
Cuts at: 3502 3502
Size: 6085
Bme1580I G_kGCm'C
Cuts at: 1790 2525 4146 5302 1790
Size: 735 1621 1156 2573
Fragments arranged by size:
2573 1621 1156 735
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3059 3816 4774
Size: 416 53 83 399 24 1642 757 958
Cuts at: 4774 442
Size: 1753
Fragments arranged by size:
1753 1642 958 757 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2335 2372 2884 4745 1239
Size: 983 113 37 512 1861 2579
Fragments arranged by size:
2579 1861 983 512 113 37
Bpu10I CC'TnA_GC
Cuts at: 2150 2150
Size: 6085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4210 5075 5373 4210
Size: 865 298 4922
Fragments arranged by size:
4922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2070 2168 3266 4748 882
Size: 617 571 98 1098 1482 2219
Fragments arranged by size:
2219 1482 1098 617 571 98
BsaAI yAC'GTr
Cuts at: 493 1204 3876 493
Size: 711 2672 2702
Fragments arranged by size:
2702 2672 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3892 4274 4909 275
Size: 53 83 186 936 2359 382 635 1451
Fragments arranged by size:
2359 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2882 2912 1178
Size: 30 165 30 1479 30 4351
Fragments arranged by size:
4351 1479 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2837 3294 1186
Size: 1651 457 3977
Fragments arranged by size:
3977 1651 457
BseYI C'CCAG_C
Cuts at: 5288 5784 5288
Size: 496 5589
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2635 1163
Size: 573 899 4613
Fragments arranged by size:
4613 899 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 3535 4296 5324 1540
Size: 275 186 60 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2430 2525 2690 4146 4231 5302 2430
Size: 95 165 1456 85 1071 3213
Fragments arranged by size:
3213 1456 1071 165 95 85
BsmI GAATG_Cn'
Cuts at: 2625 3596 3689 2625
Size: 971 93 5021
Fragments arranged by size:
5021 971 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 2525 2690 3326 3404 4146 4231
Size: 399 640 95 165 636 78 742 85
Cuts at: 4231 5302 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 640 636 399 165 95
85 78
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 2877 3974 5704 2877
Size: 1097 1730 3258
Fragments arranged by size:
3258 1730 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3440 844
Size: 2596 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 2070 2659 3350 3972 1394
Size: 429 215 32 589 691 622 3507
Fragments arranged by size:
3507 691 622 589 429 215 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 3034 4580 4754 65
Size: 1183 1786 1546 174 1396
Fragments arranged by size:
1786 1546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4729 4916 1700
Size: 110 2919 187 2869
Fragments arranged by size:
2919 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 4145 5157 4145
Size: 1012 5073
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 3343 1184
Size: 2159 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 2759 3415 3782 4167 4184 5636 5722 5734
Size: 787 656 367 385 17 1452 86 12
Cuts at: 5734 6080 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 787 656 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2229 2921 513
Size: 552 1164 692 3677
Fragments arranged by size:
3677 1164 692 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2629 487
Size: 683 56 1403 3943
Fragments arranged by size:
3943 1403 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 3078 3093 3326 3601 4395 4415 2162
Size: 916 15 233 275 794 20 3832
Fragments arranged by size:
3832 916 794 275 233 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DraIII CAC_nnn'GTG
Cuts at: 2520 3173 2520
Size: 653 5432
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2601 3532
Size: 54 1425 50 275 18 228 543 931
Cuts at: 3532 4421 6050 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 931 889 543 275 228 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 3532 1537
Size: 275 246 1474 4090
Fragments arranged by size:
4090 1474 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2630 2691 2952 4015 1353
Size: 1277 61 261 1063 3423
Fragments arranged by size:
3423 1277 1063 261 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2088 4672 5046 5192 387
Size: 1064 138 499 2584 374 146 1280
Fragments arranged by size:
2584 1280 1064 499 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2167 2222 2261 2335 2372 2884
Size: 393 503 32 55 39 74 37 512
Cuts at: 2884 4148 4745 5532 5800 1239
Size: 1264 597 787 268 1524
Fragments arranged by size:
1524 1264 787 597 512 503 393 268
74 55 39 37 32
EcoO109I rG'GnC_Cy
Cuts at: 2236 2572 2596 2236
Size: 336 24 5725
Fragments arranged by size:
5725 336 24
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
FspI TGC'GCA
Cuts at: 2306 4591 2306
Size: 2285 3800
HaeII r_GCGC'y
Cuts at: 1678 2229 5232 1678
Size: 551 3003 2531
Fragments arranged by size:
3003 2531 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2658 2690 2789 2821 4726
Size: 32 135 32 1086 32 99 32 1905
Cuts at: 4726 4758 4800 4832 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1905 1086 135 99 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 3429 3675 669
Size: 988 281 439 1052 246 3079
Fragments arranged by size:
3079 1052 988 439 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 2377 3675 2377
Size: 1298 4787
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5199 5383 1305
Size: 3894 184 2007
Fragments arranged by size:
3894 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4263
Fragments arranged by size:
4263 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 2531 2876 4043 4402 4561 518
Size: 648 1254 111 345 1167 359 159 2042
Fragments arranged by size:
2042 1254 1167 648 359 345 159 111
MspA1I CmG'CkG
Cuts at: 1606 2268 2817 3501 4178 5326 5571 1606
Size: 662 549 684 677 1148 245 2120
Fragments arranged by size:
2120 1148 684 677 662 549 245
NcoI C'CATG_G
Cuts at: 513 1065 2229 2921 513
Size: 552 1164 692 3677
Fragments arranged by size:
3677 1164 692 552
NdeI CA'TA_TG
Cuts at: 387 2365 387
Size: 1978 4107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 2323 3000 3443 4988 1132
Size: 97 1094 677 443 1545 2229
Fragments arranged by size:
2229 1545 1094 677 443 97
PasI CC'CwG_GG
Cuts at: 1348 3121 1348
Size: 1773 4312
PciI A'CATG_T
Cuts at: 2996 4984 2996
Size: 1988 4097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3314 1108
Size: 2206 3879
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2596 2596
Size: 6085
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PstI C_TGCA'G
Cuts at: 830 2132 2337 2759 2936 3437 830
Size: 1302 205 422 177 501 3478
Fragments arranged by size:
3478 1302 501 422 205 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2817 2817
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SapI GCTCTTCn'nnn_
Cuts at: 2630 2630
Size: 6085
SbfI CC_TGCA'GG
Cuts at: 2337 3437 2337
Size: 1100 4985
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 2128 2333 2755 2932 3433 4568 5249
Size: 219 1083 205 422 177 501 1135 681
Cuts at: 5249 5440 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1083 681 501 422 219 205
191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4189 5090 5352 820
Size: 197 926 2246 901 262 1553
Fragments arranged by size:
2246 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 2468 1517
Size: 951 5134
StyI C'CwwG_G
Cuts at: 513 1065 2208 2229 2921 3524 513
Size: 552 1143 21 692 603 3074
Fragments arranged by size:
3074 1143 692 603 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4112 4129 4282 4467 4912 325
Size: 1255 2532 17 153 185 445 1498
Fragments arranged by size:
2532 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 2496 3067
Size: 274 80 33 51 1443 45 474 571
Cuts at: 3067 4331 6043 96
Size: 1264 1712 138
Fragments arranged by size:
1712 1443 1264 571 474 274 138 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3318 3350 6045 6077 3318
Size: 32 2695 32 3326
Fragments arranged by size:
3326 2695 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2594 1316
Size: 1278 4807
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 3292 2185
Size: 1107 4978
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 2981 4214 2981
Size: 1233 4852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3893 276
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI