Restriction map for
pFN21AE1552
(Circular) (Six-base) MAPSORT of: pFN21AE1552.seq Check: 8166 from: 1 to: 6385
pFN21AE1552 6385 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3524 3524
Size: 6385
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3621 3728 1937
Size: 1684 107 4594
Fragments arranged by size:
4594 1684 107
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3164 3479 4448 5832 6100 1632
Size: 1532 315 969 1384 268 1917
Fragments arranged by size:
1917 1532 1384 969 315 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 2691 5284 6261 1203
Size: 1488 2593 977 1327
Fragments arranged by size:
2593 1488 1327 977
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 5114 5114
Size: 6385
AleI CACnn'nnGTG
Cuts at: 1166 3042 1166
Size: 1876 4509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6385
AlwNI CAG_nnn'CTG
Cuts at: 3142 3397 4128 5700 3142
Size: 255 731 1572 3827
Fragments arranged by size:
3827 1572 731 255
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 2558 3155 3638 3694 3920 4072 6181 1493
Size: 1065 597 483 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 1065 597 483 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 3710 1870
Size: 73 1767 4545
Fragments arranged by size:
4545 1767 73
BamHI G'GATC_C
Cuts at: 3715 4082 3715
Size: 367 6018
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3560 3706 5161 618
Size: 325 217 627 1773 146 1455 1842
Fragments arranged by size:
1842 1773 1455 627 325 217 146
BanII G_rGCy'C
Cuts at: 1391 3704 1391
Size: 2313 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3305 928
Size: 971 1406 4008
Fragments arranged by size:
4008 1406 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4576 4610 1903
Size: 34 2639 34 3678
Fragments arranged by size:
3678 2639 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6385
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4996 136
Size: 107 122 71 631 605 3324 1525
Fragments arranged by size:
3324 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6380 6380
Size: 6385
BlpI GC'TnA_GC
Cuts at: 2833 3802 2833
Size: 969 5416
Bme1580I G_kGCm'C
Cuts at: 1790 4446 5602 1790
Size: 2656 1156 2573
Fragments arranged by size:
2656 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6385
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4116 5074 442
Size: 416 53 83 399 24 2699 958 1753
Fragments arranged by size:
2699 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2981 3552 3617 5045 1239
Size: 1742 571 65 1428 2579
Fragments arranged by size:
2579 1742 1428 571 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3233 4510 5375 5673 3233
Size: 1277 865 298 3945
Fragments arranged by size:
3945 1277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5048 882
Size: 617 3549 2219
Fragments arranged by size:
3549 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2346 3295 4176 493
Size: 711 1142 949 881 2702
Fragments arranged by size:
2702 1142 949 881 711
BsaBI GATnn'nnATC
Cuts at: 2151 6379 2151
Size: 4228 2157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2990 4192 4574 5209
Size: 53 83 186 936 1457 1202 382 635
Cuts at: 5209 275
Size: 1451
Fragments arranged by size:
1457 1451 1202 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4818 5216 5490 5637 6048 4818
Size: 398 274 147 411 5155
Fragments arranged by size:
5155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2645 2675 1178
Size: 30 165 30 1242 30 4888
Fragments arranged by size:
4888 1242 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2660 1186
Size: 1474 4911
BseYI C'CCAG_C
Cuts at: 2438 3069 3357 3588 5588 6084 2438
Size: 631 288 231 2000 496 2739
Fragments arranged by size:
2739 2000 631 496 288 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3076 3504 1163
Size: 573 1340 428 4044
Fragments arranged by size:
4044 1340 573 428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3835 4596 5624 1540
Size: 275 186 1834 761 1028 2301
Fragments arranged by size:
2301 1834 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2285 2966 3078 3280 4446 4531 5602 2285
Size: 681 112 202 1166 85 1071 3068
Fragments arranged by size:
3068 1166 1071 681 202 112 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2966 3078 3280 3704 4446 4531
Size: 399 495 681 112 202 424 742 85
Cuts at: 4531 5602 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 681 495 424 399 202
112 85
BspEI T'CCGG_A
Cuts at: 6048 6048
Size: 6385
BspHI T'CATG_A
Cuts at: 3160 4274 6004 3160
Size: 1114 1730 3541
Fragments arranged by size:
3541 1730 1114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2504 3524 3740 844
Size: 1660 1020 216 3489
Fragments arranged by size:
3489 1660 1020 216
BsrBI CCG'CTC
Cuts at: 1394 1823 4272 1394
Size: 429 2449 3507
Fragments arranged by size:
3507 2449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3242 4880 5054 65
Size: 1183 1994 1638 174 1396
Fragments arranged by size:
1994 1638 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5029 5216 1700
Size: 110 3219 187 2869
Fragments arranged by size:
3219 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6385
BssSI C'ACGA_G
Cuts at: 4445 5457 4445
Size: 1012 5373
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6385
BstBI TT'CG_AA
Cuts at: 5189 5189
Size: 6385
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6385
BstXI CCAn_nnnn'nTGG
Cuts at: 2723 3044 3353 2723
Size: 321 309 5755
Fragments arranged by size:
5755 321 309
BstYI r'GATC_y
Cuts at: 1972 2516 3715 4082 4467 4484 5936 6022 6034
Size: 544 1199 367 385 17 1452 86 12
Cuts at: 6034 6380 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1199 544 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2275 2716 2981 513
Size: 552 1210 441 265 3917
Fragments arranged by size:
3917 1210 552 441 265
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2610 487
Size: 683 56 1384 4262
Fragments arranged by size:
4262 1384 683 56
BtsI GCAGTG_nn'
Cuts at: 3078 3088 3901 4695 4715 3078
Size: 10 813 794 20 4748
Fragments arranged by size:
4748 813 794 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2344 2379 529
Size: 35 1780 35 4535
Fragments arranged by size:
4535 1780 35 35
DraI TTT'AAA
Cuts at: 3689 3689
Size: 6385
DraIII CAC_nnn'GTG
Cuts at: 3432 3432
Size: 6385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2270 5392 809
Size: 507 954 3122 1802
Fragments arranged by size:
3122 1802 954 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2365 3832 4721
Size: 54 1425 50 275 18 535 1467 889
Cuts at: 4721 6350 8
Size: 1629 43
Fragments arranged by size:
1629 1467 1425 889 535 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 3602 4315 1353
Size: 801 1448 713 3423
Fragments arranged by size:
3423 1448 801 713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4972 5346 5492 387
Size: 1064 138 3383 374 146 1280
Fragments arranged by size:
3383 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2981 3164 3479 3552 3617 4448 5045
Size: 393 1349 183 315 73 65 831 597
Cuts at: 5045 5832 6100 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1349 831 787 597 393 315 268
183 73 65
EcoNI CCTnn'n_nnAGG
Cuts at: 2430 2675 2430
Size: 245 6140
EcoO109I rG'GnC_Cy
Cuts at: 2435 3139 3321 3384 3536 3592 2435
Size: 704 182 63 152 56 5228
Fragments arranged by size:
5228 704 182 152 63 56
EcoRI G'AATT_C
Cuts at: 3694 4072 3694
Size: 378 6007
FspI TGC'GCA
Cuts at: 4891 4891
Size: 6385
HaeII r_GCGC'y
Cuts at: 1678 3056 5532 1678
Size: 1378 2476 2531
Fragments arranged by size:
2531 2476 1378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2588 2620 5026
Size: 32 135 32 436 32 548 32 2406
Cuts at: 5026 5058 5100 5132 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2406 548 436 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 3729 3975 669
Size: 988 281 697 1094 246 3079
Fragments arranged by size:
3079 1094 988 697 281 246
HindIII A'AGCT_T
Cuts at: 748 2885 748
Size: 2137 4248
HpaI GTT'AAC
Cuts at: 3975 3975
Size: 6385
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5499 5683 1305
Size: 4194 184 2007
Fragments arranged by size:
4194 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4563
Fragments arranged by size:
4563 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 3023 3042 3207 4343 4702 4861
Size: 648 1530 327 19 165 1136 359 159
Cuts at: 4861 518
Size: 2042
Fragments arranged by size:
2042 1530 1136 648 359 327 165 159
19
MspA1I CmG'CkG
Cuts at: 1606 2298 2409 2442 3801 4478 5626 5871 1606
Size: 692 111 33 1359 677 1148 245 2120
Fragments arranged by size:
2120 1359 1148 692 677 245 111 33
NcoI C'CATG_G
Cuts at: 513 1065 2275 2716 2981 513
Size: 552 1210 441 265 3917
Fragments arranged by size:
3917 1210 552 441 265
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 2295 2645 2695 3743 5288 1132
Size: 97 1066 350 50 1048 1545 2229
Fragments arranged by size:
2229 1545 1066 1048 350 97 50
PasI CC'CwG_GG
Cuts at: 1348 3031 1348
Size: 1683 4702
PciI A'CATG_T
Cuts at: 2691 5284 2691
Size: 2593 3792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3266 3397 1108
Size: 2158 131 4096
Fragments arranged by size:
4096 2158 131
PfoI T'CCnGG_A
Cuts at: 3396 3396
Size: 6385
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 5967 5999 4454
Size: 32 1481 32 4840
Fragments arranged by size:
4840 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2435 3139 3321 3384 2435
Size: 704 182 63 5436
Fragments arranged by size:
5436 704 182 63
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6385
PsiI TTA'TAA
Cuts at: 2151 3955 2151
Size: 1804 4581
PstI C_TGCA'G
Cuts at: 830 2744 3737 830
Size: 1914 993 3478
Fragments arranged by size:
3478 1914 993
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 2298 2442 2298
Size: 144 6241
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SanDI GG'GwC_CC
Cuts at: 3384 3384
Size: 6385
SbfI CC_TGCA'GG
Cuts at: 3737 3737
Size: 6385
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SfcI C'TryA_G
Cuts at: 826 1045 2740 3733 4868 5549 5740 826
Size: 219 1695 993 1135 681 191 1471
Fragments arranged by size:
1695 1471 1135 993 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6385
SmaI CCC'GGG
Cuts at: 1872 3712 1872
Size: 1840 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3248 4489 5390 5652 820
Size: 197 926 1305 1241 901 262 1553
Fragments arranged by size:
1553 1305 1241 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 2645 3743 1132
Size: 1513 1098 3774
Fragments arranged by size:
3774 1513 1098
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6385
StyI C'CwwG_G
Cuts at: 513 1065 2275 2716 2981 3824 513
Size: 552 1210 441 265 843 3074
Fragments arranged by size:
3074 1210 843 552 441 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2994 3061 3641 4412 4429 4582 4767
Size: 1255 1414 67 580 771 17 153 185
Cuts at: 4767 5212 325
Size: 445 1498
Fragments arranged by size:
1498 1414 1255 771 580 445 185 153
67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3195 4631 6343
Size: 274 80 33 51 1443 1218 1436 1712
Cuts at: 6343 96
Size: 138
Fragments arranged by size:
1712 1443 1436 1218 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2548 2580 6345 6377 2548
Size: 32 3765 32 2556
Fragments arranged by size:
3765 2556 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6385
XbaI T'CTAG_A
Cuts at: 3280 3721 3280
Size: 441 5944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3044 3527 3044
Size: 483 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6385
XmaI C'CCGG_G
Cuts at: 1870 3710 1870
Size: 1840 4545
XmnI GAAnn'nnTTC
Cuts at: 3156 3188 4514 3156
Size: 32 1326 5027
Fragments arranged by size:
5027 1326 32
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI