Restriction map for
pFN21AE1550
(Circular) (Six-base) MAPSORT of: pFN21AE1550.seq Check: 7108 from: 1 to: 7246
pFN21AE1550 7246 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5056 278
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4589 1937
Size: 2652 4594
Acc65I G'GTAC_C
Cuts at: 3541 4567 3541
Size: 1026 6220
AclI AA'CG_TT
Cuts at: 1563 2293 2367 5373 5746 1563
Size: 730 74 3006 373 3063
Fragments arranged by size:
3063 3006 730 373 74
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2739 3233 3513 4286 5309 6693 6961
Size: 710 397 494 280 773 1023 1384 268
Cuts at: 6961 1632
Size: 1917
Fragments arranged by size:
1917 1384 1023 773 710 494 397 280
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7049
AflIII A'CryG_T
Cuts at: 1203 6145 7122 1203
Size: 4942 977 1327
Fragments arranged by size:
4942 1327 977
AgeI A'CCGG_T
Cuts at: 4074 6077 4074
Size: 2003 5243
AhdI GACnn_n'nnGTC
Cuts at: 5975 5975
Size: 7246
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2205 1184
Size: 1021 6225
AlwNI CAG_nnn'CTG
Cuts at: 2128 2308 2503 2524 2768 3712 3812 4989 6561
Size: 180 195 21 244 944 100 1177 1572
Cuts at: 6561 2128
Size: 2813
Fragments arranged by size:
2813 1572 1177 944 244 195 180 100
21
ApaI G_GGCC'C
Cuts at: 2362 2362
Size: 7246
ApaLI G'TGCA_C
Cuts at: 3274 5303 6459 3274
Size: 2029 1156 4061
Fragments arranged by size:
4061 2029 1156
ApoI r'AATT_y
Cuts at: 1493 2630 2645 4555 4781 4933 7042 1493
Size: 1137 15 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 1137 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 5800 160
Size: 872 4768 1606
Fragments arranged by size:
4768 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 4571 1870
Size: 73 196 2432 4545
Fragments arranged by size:
4545 2432 196 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4136 4169 4136
Size: 33 7213
BamHI G'GATC_C
Cuts at: 3424 4576 4943 3424
Size: 1152 367 5727
Fragments arranged by size:
5727 1152 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4567 6022 618
Size: 325 217 627 1754 1026 1455 1842
Fragments arranged by size:
1842 1754 1455 1026 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2362 2919 4565 1391
Size: 971 557 1646 4072
Fragments arranged by size:
4072 1646 971 557
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2330 3746 928
Size: 971 431 1416 4428
Fragments arranged by size:
4428 1416 971 431
BbvCI CC'TCA_GC
Cuts at: 2730 3005 4233 2730
Size: 275 1228 5743
Fragments arranged by size:
5743 1228 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3608 3642 3989 4023 5437 5471 1903
Size: 34 1671 34 347 34 1414 34 3678
Fragments arranged by size:
3678 1671 1414 347 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2218 3981 4137 5138 6348 2218
Size: 1763 156 1001 1210 3116
Fragments arranged by size:
3116 1763 1210 1001 156
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7246
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3635 5857 136
Size: 107 122 71 631 605 1963 2222 1525
Fragments arranged by size:
2222 1963 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2580 7241 2580
Size: 4661 2585
BlpI GC'TnA_GC
Cuts at: 4663 4663
Size: 7246
Bme1580I G_kGCm'C
Cuts at: 1790 2362 3278 5307 6463 1790
Size: 572 916 2029 1156 2573
Fragments arranged by size:
2573 2029 1156 916 572
BmgBI CAC'GTC
Cuts at: 1318 4214 1318
Size: 2896 4350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4977 5935 442
Size: 416 53 83 399 24 3560 958 1753
Fragments arranged by size:
3560 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2379 1056
Size: 1323 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 7246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2956 3033 3569 3662 4295 4470 5906
Size: 1016 701 77 536 93 633 175 1436
Cuts at: 5906 1239
Size: 2579
Fragments arranged by size:
2579 1436 1016 701 633 536 175 93
77
Bpu10I CC'TnA_GC
Cuts at: 2730 2918 3005 4233 4239 2730
Size: 188 87 1228 6 5737
Fragments arranged by size:
5737 1228 188 87 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 5371 6236 6534 4465
Size: 906 865 298 5177
Fragments arranged by size:
5177 906 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4044 5909 882
Size: 617 2545 1865 2219
Fragments arranged by size:
2545 2219 1865 617
BsaAI yAC'GTr
Cuts at: 493 1204 5037 493
Size: 711 3833 2702
Fragments arranged by size:
3833 2702 711
BsaBI GATnn'nnATC
Cuts at: 7240 7240
Size: 7246
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5053 5435 6070 275
Size: 53 83 186 936 3520 382 635 1451
Fragments arranged by size:
3520 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4108 4140 4185 5679 6077 6351 6498 6909
Size: 34 32 45 1494 398 274 147 411
Cuts at: 6909 4074
Size: 4411
Fragments arranged by size:
4411 1494 411 398 274 147 45 34
32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2789 2819 2906 2936 1178
Size: 30 165 30 1386 30 87 30 5488
Fragments arranged by size:
5488 1386 165 87 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3050 3092 3095 3104 3107 3110 3446 3527
Size: 1864 42 3 9 3 3 336 81
Cuts at: 3527 3530 3533 3536 3539 1186
Size: 3 3 3 3 4893
Fragments arranged by size:
4893 1864 336 81 42 9 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2379 3611 3657 3891 3973 4370 6449 6945 2379
Size: 1232 46 234 82 397 2079 496 2680
Fragments arranged by size:
2680 2079 1232 496 397 234 82 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3348 1163
Size: 573 1612 5061
Fragments arranged by size:
5061 1612 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4696 5457 6485 1540
Size: 275 186 2695 761 1028 2301
Fragments arranged by size:
2695 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 2919 3278 4507 5307 5392 6463 2349
Size: 570 359 1229 800 85 1071 3132
Fragments arranged by size:
3132 1229 1071 800 570 359 85
BsmI GAATG_Cn'
Cuts at: 4757 4850 4757
Size: 93 7153
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 2060
Size: 7246
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2362 2919 3278 4507 4565 5307
Size: 399 559 13 557 359 1229 58 742
Cuts at: 5307 5392 6463 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1229 1071 742 559 557 399 359
85 58 13
BspEI T'CCGG_A
Cuts at: 4108 6909 4108
Size: 2801 4445
BspHI T'CATG_A
Cuts at: 5135 6865 5135
Size: 1730 5516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2173 4601 844
Size: 1329 2428 3489
Fragments arranged by size:
3489 2428 1329
BsrBI CCG'CTC
Cuts at: 1394 1823 5133 1394
Size: 429 3310 3507
Fragments arranged by size:
3507 3310 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3237 5741 5915 65
Size: 1183 1989 2504 174 1396
Fragments arranged by size:
2504 1989 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3675 4074 4440 5890 6077 1700
Size: 110 1865 399 366 1450 187 2869
Fragments arranged by size:
2869 1865 1450 399 366 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7246
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7246
BssSI C'ACGA_G
Cuts at: 2487 5306 6318 2487
Size: 2819 1012 3415
Fragments arranged by size:
3415 2819 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3712 794
Size: 2918 4328
BstBI TT'CG_AA
Cuts at: 6050 6050
Size: 7246
BstEII G'GTnAC_C
Cuts at: 1184 2072 2735 3287 1184
Size: 888 663 552 5143
Fragments arranged by size:
5143 888 663 552
BstXI CCAn_nnnn'nTGG
Cuts at: 2175 2175
Size: 7246
BstYI r'GATC_y
Cuts at: 1972 2511 2580 3220 3424 4576 4943 5328 5345
Size: 539 69 640 204 1152 367 385 17
Cuts at: 5345 6797 6883 6895 7241 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1152 640 539 385 367 346
204 86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 2309 3780 2309
Size: 1471 5775
BtgI C'CryG_G
Cuts at: 513 1065 2004 2452 3904 3922 4302 513
Size: 552 939 448 1452 18 380 3457
Fragments arranged by size:
3457 1452 939 552 448 380 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6507
Fragments arranged by size:
6507 683 56
BtsI GCAGTG_nn'
Cuts at: 3533 3880 4762 5556 5576 3533
Size: 347 882 794 20 5203
Fragments arranged by size:
5203 882 794 347 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3805 3840 529
Size: 35 3241 35 3935
Fragments arranged by size:
3935 3241 35 35
DraI TTT'AAA
Cuts at: 4550 4550
Size: 7246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3459 6253 809
Size: 507 2143 2794 1802
Fragments arranged by size:
2794 2143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2961 4442 4693
Size: 54 1425 50 275 18 1131 1481 251
Cuts at: 4693 5582 7211 8
Size: 889 1629 43
Fragments arranged by size:
1629 1481 1425 1131 889 275 251 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4693 1537
Size: 275 2881 4090
Fragments arranged by size:
4090 2881 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2832 3081 3149 3879 4036 4262 5176 1353
Size: 1479 249 68 730 157 226 914 3423
Fragments arranged by size:
3423 1479 914 730 249 226 157 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 3161 3694 5833 6207 6353
Size: 1064 138 930 642 533 2139 374 146
Cuts at: 6353 387
Size: 1280
Fragments arranged by size:
2139 1280 1064 930 642 533 374 146
138
EcoICRI GAG'CTC
Cuts at: 2917 2917
Size: 7246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2342 2739 2956 3033 3233 3513
Size: 393 623 87 397 217 77 200 280
Cuts at: 3513 3569 3662 4286 4295 4470 5309 5906 6693
Size: 56 93 624 9 175 839 597 787
Cuts at: 6693 6961 1239
Size: 268 1524
Fragments arranged by size:
1524 839 787 624 623 597 397 393
280 268 217 200 175 93 87 77
56 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2312 2807 2312
Size: 495 6751
EcoO109I rG'GnC_Cy
Cuts at: 2305 2358 2359 3637 3970 2305
Size: 53 1 1278 333 5581
Fragments arranged by size:
5581 1278 333 53 1
EcoRI G'AATT_C
Cuts at: 4555 4933 4555
Size: 378 6868
EcoRV GAT'ATC
Cuts at: 3367 3367
Size: 7246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3622 3622
Size: 7246
FspI TGC'GCA
Cuts at: 5752 5752
Size: 7246
HaeII r_GCGC'y
Cuts at: 1678 6393 1678
Size: 4715 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2789 2821 2906
Size: 32 135 32 809 32 376 32 85
Cuts at: 2906 2938 3389 3421 3737 3739 3769 3771 3833
Size: 32 451 32 316 2 30 2 62
Cuts at: 3833 3865 5887 5919 5961 5993 1373
Size: 32 2022 32 42 32 2626
Fragments arranged by size:
2626 2022 809 451 376 316 135 85
62 42 32 32 32 32 32 32
32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2623 2797 4590 4836 669
Size: 988 281 685 174 1793 246 3079
Fragments arranged by size:
3079 1793 988 685 281 246 174
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7246
HpaI GTT'AAC
Cuts at: 4836 4836
Size: 7246
KpnI G_GTAC'C
Cuts at: 3545 4571 3545
Size: 1026 6220
MfeI C'AATT_G
Cuts at: 4845 4845
Size: 7246
MluI A'CGCG_T
Cuts at: 7122 7122
Size: 7246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6360 6544 1305
Size: 5055 184 2007
Fragments arranged by size:
5055 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2963 10
Size: 54 1425 343 1131 4293
Fragments arranged by size:
4293 1425 1131 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 2897 5204 5563 5722 518
Size: 648 1710 21 2307 359 159 2042
Fragments arranged by size:
2307 2042 1710 648 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2524 3050 3196 3661 3712 4348 4448 4662
Size: 918 526 146 465 51 636 100 214
Cuts at: 4662 5339 6487 6732 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 918 677 636 526 465 245
214 146 100 51
NaeI GCC'GGC
Cuts at: 4442 4442
Size: 7246
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5755
Fragments arranged by size:
5755 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7246
NgoMIV G'CCGG_C
Cuts at: 4440 4440
Size: 7246
NheI G'CTAG_C
Cuts at: 1052 2375 1052
Size: 1323 5923
NotI GC'GGCC_GC
Cuts at: 4693 4693
Size: 7246
NspI r_CATG'y
Cuts at: 1132 1229 3265 4604 6149 1132
Size: 97 2036 1339 1545 2229
Fragments arranged by size:
2229 2036 1545 1339 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7246
PciI A'CATG_T
Cuts at: 6145 6145
Size: 7246
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 3142 3588 1108
Size: 2010 24 446 4766
Fragments arranged by size:
4766 2010 446 24
PfoI T'CCnGG_A
Cuts at: 2271 2271
Size: 7246
PmeI GTTT'AAAC
Cuts at: 4550 4550
Size: 7246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5315 5347 6828 6860 5315
Size: 32 1481 32 5701
Fragments arranged by size:
5701 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3970 3970
Size: 7246
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7246
PsiI TTA'TAA
Cuts at: 4816 4816
Size: 7246
PspOMI G'GGCC_C
Cuts at: 2358 2358
Size: 7246
PstI C_TGCA'G
Cuts at: 830 2133 2184 2578 3570 4598 830
Size: 1303 51 394 992 1028 3478
Fragments arranged by size:
3478 1303 1028 992 394 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7246
PvuII CAG'CTG
Cuts at: 2524 3050 3661 3712 4348 4448 2524
Size: 526 611 51 636 100 5322
Fragments arranged by size:
5322 636 611 526 100 51
SacI G_AGCT'C
Cuts at: 2919 2919
Size: 7246
SalI G'TCGA_C
Cuts at: 1936 4588 1936
Size: 2652 4594
SbfI CC_TGCA'GG
Cuts at: 4598 4598
Size: 7246
ScaI AGT'ACT
Cuts at: 3564 3868 5494 7206 3564
Size: 304 1626 1712 3604
Fragments arranged by size:
3604 1712 1626 304
SexAI A'CCwGG_T
Cuts at: 2232 2232
Size: 7246
SfcI C'TryA_G
Cuts at: 826 1045 2129 2180 2574 3566 4594 5729 6410
Size: 219 1084 51 394 992 1028 1135 681
Cuts at: 6410 6601 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1084 1028 992 681 394 219
191 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7246
SmaI CCC'GGG
Cuts at: 1872 4573 1872
Size: 2701 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4480 5350 6251 6513 820
Size: 197 926 2537 870 901 262 1553
Fragments arranged by size:
2537 1553 926 901 870 262 197
SnaBI TAC'GTA
Cuts at: 493 5037 493
Size: 4544 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7246
SphI G_CATG'C
Cuts at: 1132 4604 1132
Size: 3472 3774
SspI AAT'ATT
Cuts at: 5 52 5170 5
Size: 47 5118 2081
Fragments arranged by size:
5118 2081 47
StuI AGG'CCT
Cuts at: 1517 4005 1517
Size: 2488 4758
StyI C'CwwG_G
Cuts at: 513 1065 2004 2596 2808 3117 3356 4685 513
Size: 552 939 592 212 309 239 1329 3074
Fragments arranged by size:
3074 1329 939 592 552 309 239 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5273 5290 5443 5628 6073 325
Size: 1255 3693 17 153 185 445 1498
Fragments arranged by size:
3693 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3562 3866 5492
Size: 274 80 33 51 1443 1585 304 1626
Cuts at: 5492 7204 96
Size: 1712 138
Fragments arranged by size:
1712 1626 1585 1443 304 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7206 7238 7206
Size: 32 7214
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7246
XbaI T'CTAG_A
Cuts at: 4582 4582
Size: 7246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2218 2821 4225 4420 2218
Size: 603 1404 195 5044
Fragments arranged by size:
5044 1404 603 195
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7246
XmaI C'CCGG_G
Cuts at: 1870 4571 1870
Size: 2701 4545
XmnI GAAnn'nnTTC
Cuts at: 5375 5375
Size: 7246
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5054 276
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI