Restriction map for
pFN21AE1549
(Circular) (Six-base) MAPSORT of: pFN21AE1549.seq Check: 3090 from: 1 to: 6232
pFN21AE1549 6232 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 2412 3200 3575 1937
Size: 208 267 788 375 4594
Fragments arranged by size:
4594 788 375 267 208
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4295 5679 5947 1632
Size: 2663 1384 268 1917
Fragments arranged by size:
2663 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 3975 5547 3975
Size: 1572 4660
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 2270 3103 3379 3434 3541 3767 3919 6028
Size: 777 833 276 55 107 226 152 2109
Cuts at: 6028 1493
Size: 1697
Fragments arranged by size:
2109 1697 833 777 276 226 152 107
55
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 3557 1870
Size: 73 1614 4545
Fragments arranged by size:
4545 1614 73
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3553 5008 618
Size: 325 217 627 1766 1455 1842
Fragments arranged by size:
1842 1766 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2625 2791 3551 1391
Size: 1234 166 760 4072
Fragments arranged by size:
4072 1234 760 166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2397 928
Size: 971 498 4763
Fragments arranged by size:
4763 971 498
BbvCI CC'TCA_GC
Cuts at: 2135 2135
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4124 5334 3446
Size: 678 1210 4344
Fragments arranged by size:
4344 1210 678
BclI T'GATC_A
Cuts at: 1548 3156 1548
Size: 1608 4624
BfrBI ATG'CAT
Cuts at: 2285 2285
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4843 136
Size: 107 122 71 631 605 337 2834 1525
Fragments arranged by size:
2834 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 4293 5449 1790
Size: 2503 1156 2573
Fragments arranged by size:
2573 2503 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3497 3963 4921
Size: 416 53 83 399 24 2080 466 958
Cuts at: 4921 442
Size: 1753
Fragments arranged by size:
2080 1753 958 466 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2643 1056
Size: 1587 4645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3195 4892 1239
Size: 1956 1697 2579
Fragments arranged by size:
2579 1956 1697
Bpu10I CC'TnA_GC
Cuts at: 2135 2135
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2829 3077 4357 5222 5520 2829
Size: 248 1280 865 298 3541
Fragments arranged by size:
3541 1280 865 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2902 3358 4039 4421
Size: 53 83 186 936 1369 456 681 382
Cuts at: 4421 5056 275
Size: 635 1451
Fragments arranged by size:
1451 1369 936 681 635 456 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 3193 3223 1178
Size: 30 165 30 798 30 962 30 4187
Fragments arranged by size:
4187 962 798 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3171 1186
Size: 1985 4247
BseYI C'CCAG_C
Cuts at: 2018 2032 2062 3313 3353 5435 5931 2018
Size: 14 30 1251 40 2082 496 2319
Fragments arranged by size:
2319 2082 1251 496 40 30 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 1163
Size: 573 613 5046
Fragments arranged by size:
5046 613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2625 2791 4293 4378 5449 2625
Size: 166 1502 85 1071 3408
Fragments arranged by size:
3408 1502 1071 166 85
BsmI GAATG_Cn'
Cuts at: 2404 3743 3836 2404
Size: 1339 93 4800
Fragments arranged by size:
4800 1339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2625 2791 3551 4293 4378 5449 1391
Size: 399 835 166 760 742 85 1071 2174
Fragments arranged by size:
2174 1071 835 760 742 399 166 85
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 3159 4121 5851 3159
Size: 962 1730 3540
Fragments arranged by size:
3540 1730 962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 844
Size: 2743 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4727 4901 65
Size: 1183 3479 174 1396
Fragments arranged by size:
3479 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 4876 5063 1700
Size: 110 355 2711 187 2869
Fragments arranged by size:
2869 2711 355 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6232
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 3097 3562 3929 4314 4331 5783 5869 5881
Size: 1125 465 367 385 17 1452 86 12
Cuts at: 5881 6227 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1125 465 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3201 3201
Size: 6232
BtgI C'CryG_G
Cuts at: 513 1065 2004 2319 513
Size: 552 939 315 4426
Fragments arranged by size:
4426 939 552 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2087 487
Size: 683 56 861 4632
Fragments arranged by size:
4632 861 683 56
BtsI GCAGTG_nn'
Cuts at: 2366 3748 4542 4562 2366
Size: 1382 794 20 4036
Fragments arranged by size:
4036 1382 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 2527 3536 2527
Size: 1009 5223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3409 5239 809
Size: 507 2093 1830 1802
Fragments arranged by size:
2093 1830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2167 3003
Size: 54 1425 50 275 18 330 7 836
Cuts at: 3003 3171 3679 4568 6197 8
Size: 168 508 889 1629 43
Fragments arranged by size:
1629 1425 889 836 508 330 275 168
54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4162 1353
Size: 2809 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2623 2789 2623
Size: 166 6066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3195 4295 4892 5679 5947 1239
Size: 393 1563 1100 597 787 268 1524
Fragments arranged by size:
1563 1524 1100 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 6232
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 5379 1678
Size: 3701 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4873 4905 4947 4979 1373
Size: 32 135 32 3301 32 42 32 2626
Fragments arranged by size:
3301 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2675 3576 3822 669
Size: 988 281 737 901 246 3079
Fragments arranged by size:
3079 988 901 737 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 2265 3831 2265
Size: 1566 4666
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5346 5530 1305
Size: 4041 184 2007
Fragments arranged by size:
4041 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3005 3173 10
Size: 54 1425 343 1173 168 3069
Fragments arranged by size:
3069 1425 1173 343 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4190 4549 4708 518
Size: 648 3024 359 159 2042
Fragments arranged by size:
3024 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2698 3648 4325 5473 5718 1606
Size: 576 516 950 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 677 576 516 245
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 6232
NcoI C'CATG_G
Cuts at: 513 1065 2004 2319 513
Size: 552 939 315 4426
Fragments arranged by size:
4426 939 552 315
NdeI CA'TA_TG
Cuts at: 387 2287 387
Size: 1900 4332
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 2639 1052
Size: 1587 4645
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NsiI A_TGCA'T
Cuts at: 2287 2287
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2243 2537 3590 5135 1132
Size: 97 1014 294 1053 1545 2229
Fragments arranged by size:
2229 1545 1053 1014 294 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2074 2234 1108
Size: 966 160 5106
Fragments arranged by size:
5106 966 160
PfoI T'CCnGG_A
Cuts at: 3211 3211
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2046 3584 830
Size: 1216 1538 3478
Fragments arranged by size:
3478 1538 1216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2182 2698 2182
Size: 516 5716
SacI G_AGCT'C
Cuts at: 2625 2791 2625
Size: 166 6066
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SexAI A'CCwGG_T
Cuts at: 2172 2746 2172
Size: 574 5658
SfcI C'TryA_G
Cuts at: 826 1045 2042 2413 3580 4715 5396 5587 826
Size: 219 997 371 1167 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 997 681 371 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2844 3092 4336 5237 5499 820
Size: 197 926 901 248 1244 901 262 1553
Fragments arranged by size:
1553 1244 926 901 901 262 248 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 2978 152
Size: 2826 3406
SphI G_CATG'C
Cuts at: 1132 2243 3590 1132
Size: 1111 1347 3774
Fragments arranged by size:
3774 1347 1111
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2004 2233 2319 3671 513
Size: 552 939 229 86 1352 3074
Fragments arranged by size:
3074 1352 939 552 229 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4478 6190 96
Size: 274 80 33 51 1443 2501 1712 138
Fragments arranged by size:
2501 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3207 3239 6192 6224 3207
Size: 32 2953 32 3215
Fragments arranged by size:
3215 2953 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 2275 3568 2275
Size: 1293 4939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2651 2651
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI