Restriction map for
pFN21AE1544
(Circular) (Six-base) MAPSORT of: pFN21AE1544.seq Check: 8175 from: 1 to: 6055
pFN21AE1544 6055 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3182 4118 5502 5770 1632
Size: 1550 936 1384 268 1917
Fragments arranged by size:
1917 1550 1384 936 268
AflII C'TTAA_G
Cuts at: 820 1017 2966 820
Size: 197 1949 3909
Fragments arranged by size:
3909 1949 197
AflIII A'CryG_T
Cuts at: 1203 2737 4954 5931 1203
Size: 1534 2217 977 1327
Fragments arranged by size:
2217 1534 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2505 2537 2505
Size: 32 6023
AlwNI CAG_nnn'CTG
Cuts at: 2163 3798 5370 2163
Size: 1635 1572 2848
Fragments arranged by size:
2848 1635 1572
ApaI G_GGCC'C
Cuts at: 2591 3283 2591
Size: 692 5363
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2096 2669 3364 3590 3742 5851 1493
Size: 603 573 695 226 152 2109 1697
Fragments arranged by size:
2109 1697 695 603 573 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 1870
Size: 73 1437 4545
Fragments arranged by size:
4545 1437 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3376 4831 618
Size: 325 217 627 1589 1455 1842
Fragments arranged by size:
1842 1589 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2591 2837 3283 3374 1391
Size: 1200 246 446 91 4072
Fragments arranged by size:
4072 1200 446 246 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 2931 3170 928
Size: 971 423 609 239 3813
Fragments arranged by size:
3813 971 609 423 239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4246 4280 1903
Size: 34 2309 34 3678
Fragments arranged by size:
3678 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 2377 1548
Size: 829 5226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2591 3283 4116 5272 1790
Size: 367 434 692 833 1156 2573
Fragments arranged by size:
2573 1156 833 692 434 367
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3286 3786 4744
Size: 416 53 83 399 24 1869 500 958
Cuts at: 4744 442
Size: 1753
Fragments arranged by size:
1869 1753 958 500 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2484 2515 2597 4715 1239
Size: 1245 31 82 2118 2579
Fragments arranged by size:
2579 2118 1245 82 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2813 4180 5045 5343 2066
Size: 747 1367 865 298 2778
Fragments arranged by size:
2778 1367 865 747 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4718 882
Size: 617 3219 2219
Fragments arranged by size:
3219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2979 3846 493
Size: 711 1775 867 2702
Fragments arranged by size:
2702 1775 867 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2681 3862 4244 4879
Size: 53 83 186 936 1148 1181 382 635
Cuts at: 4879 275
Size: 1451
Fragments arranged by size:
1451 1181 1148 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2441 4488 4886 5160 5307 5718 2441
Size: 2047 398 274 147 411 2778
Fragments arranged by size:
2778 2047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2457 2487 2505 2535 2963
Size: 30 165 30 1054 30 18 30 428
Cuts at: 2963 2993 1178
Size: 30 4240
Fragments arranged by size:
4240 1054 428 165 30 30 30 30
30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 1186
Size: 1066 4989
BseYI C'CCAG_C
Cuts at: 2584 5258 5754 2584
Size: 2674 496 2885
Fragments arranged by size:
2885 2674 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2451 2837 4116 4201 5272 2451
Size: 386 1279 85 1071 3234
Fragments arranged by size:
3234 1279 1071 386 85
BsmI GAATG_Cn'
Cuts at: 2225 3566 3659 2225
Size: 1341 93 4621
Fragments arranged by size:
4621 1341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2451 2591 2837 3283 3374 4116
Size: 399 367 294 140 246 446 91 742
Cuts at: 4116 4201 5272 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 367 294 246
140 91 85
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2793 3410 844
Size: 1949 617 3489
Fragments arranged by size:
3489 1949 617
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 4699 4886 1700
Size: 110 262 2627 187 2869
Fragments arranged by size:
2869 2627 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 2725 2959 2725
Size: 234 5821
BstYI r'GATC_y
Cuts at: 1972 2962 3385 3752 4137 4154 5606 5692 5704
Size: 990 423 367 385 17 1452 86 12
Cuts at: 5704 6050 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 990 423 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2264 2264
Size: 6055
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 2170 2478 2993 3571 4365 4385 2107
Size: 63 308 515 578 794 20 3777
Fragments arranged by size:
3777 794 578 515 308 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 5062 809
Size: 507 1209 2537 1802
Fragments arranged by size:
2537 1802 1209 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2541 3225 3502
Size: 54 1425 50 275 18 711 684 277
Cuts at: 3502 4391 6020 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 711 684 277 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3985 1353
Size: 2632 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2570 4642 5016 5162 387
Size: 1064 138 981 2072 374 146 1280
Fragments arranged by size:
2072 1280 1064 981 374 146 138
EcoICRI GAG'CTC
Cuts at: 2835 2835
Size: 6055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2515 2597 3182 4118 4715 5502
Size: 393 852 31 82 585 936 597 787
Cuts at: 5502 5770 1239
Size: 268 1524
Fragments arranged by size:
1524 936 852 787 597 585 393 268
82 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2400 2400
Size: 6055
EcoO109I rG'GnC_Cy
Cuts at: 2588 2805 3280 2588
Size: 217 475 5363
Fragments arranged by size:
5363 475 217
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3157 3189 4696 4728 4770
Size: 32 135 32 1585 32 1507 32 42
Cuts at: 4770 4802 1373
Size: 32 2626
Fragments arranged by size:
2626 1585 1507 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2131 3399 3645 669
Size: 988 281 193 1268 246 3079
Fragments arranged by size:
3079 1268 988 281 246 193
HindIII A'AGCT_T
Cuts at: 748 2954 748
Size: 2206 3849
HpaI GTT'AAC
Cuts at: 2131 3645 2131
Size: 1514 4541
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3039 5169 5353 1305
Size: 1734 2130 184 2007
Fragments arranged by size:
2130 2007 1734 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2543 3227 10
Size: 54 1425 343 711 684 2838
Fragments arranged by size:
2838 1425 711 684 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2723 4013 4372 4531 518
Size: 648 1557 1290 359 159 2042
Fragments arranged by size:
2042 1557 1290 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2584 3031 3471 4148 5296 5541 1606
Size: 978 447 440 677 1148 245 2120
Fragments arranged by size:
2120 1148 978 677 447 440 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3234 3352 1108
Size: 2126 118 3811
Fragments arranged by size:
3811 2126 118
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2505 2537 4124 4156 5637 5669 2505
Size: 32 1587 32 1481 32 2891
Fragments arranged by size:
2891 1587 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2805 2805
Size: 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 2587 3279 2587
Size: 692 5363
PstI C_TGCA'G
Cuts at: 830 3407 830
Size: 2577 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2584 3031 2584
Size: 447 5608
SacI G_AGCT'C
Cuts at: 2837 2837
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 3403 4538 5219 5410 826
Size: 219 2358 1135 681 191 1471
Fragments arranged by size:
2358 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 2828 2966 4159 5060 5322
Size: 197 926 102 783 138 1193 901 262
Cuts at: 5322 820
Size: 1553
Fragments arranged by size:
1553 1193 926 901 783 262 197 138
102
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2591 3494 513
Size: 552 939 587 903 3074
Fragments arranged by size:
3074 939 903 587 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3285 4301 6013
Size: 274 80 33 51 1443 1308 1016 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
1712 1443 1308 1016 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 2963 2995 6015 6047 2457
Size: 32 474 32 3020 32 2465
Fragments arranged by size:
3020 2465 474 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2339 2945 1316
Size: 1023 606 4426
Fragments arranged by size:
4426 1023 606
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 2319 4184 2319
Size: 1865 4190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI