Restriction map for
pFN21AE1530
(Circular) (Six-base) MAPSORT of: pFN21AE1530.seq Check: 938 from: 1 to: 6043
pFN21AE1530 6043 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 3386 1937
Size: 214 1235 4594
Fragments arranged by size:
4594 1235 214
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2488 2669 3107 4106 5490 5758 1632
Size: 856 181 438 999 1384 268 1917
Fragments arranged by size:
1917 1384 999 856 438 268 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2995 3133 3786 5358 2995
Size: 138 653 1572 3680
Fragments arranged by size:
3680 1572 653 138
ApaI G_GGCC'C
Cuts at: 2854 2964 2854
Size: 110 5933
ApaLI G'TGCA_C
Cuts at: 2018 2570 4100 5256 2018
Size: 552 1530 1156 2805
Fragments arranged by size:
2805 1530 1156 552
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2731 2864 3368 1870
Size: 73 788 133 504 4545
Fragments arranged by size:
4545 788 504 133 73
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2216 2776 3364 4819
Size: 325 217 627 267 162 560 588 1455
Cuts at: 4819 618
Size: 1842
Fragments arranged by size:
1842 1455 627 588 560 325 267 217
162
BanII G_rGCy'C
Cuts at: 1391 2438 2550 2854 2871 2964 3362 1391
Size: 1047 112 304 17 93 398 4072
Fragments arranged by size:
4072 1047 398 304 112 93 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3007 3041 4234 4268 1903
Size: 34 1070 34 1193 34 3678
Fragments arranged by size:
3678 1193 1070 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3271 3935 5145 3271
Size: 664 1210 4169
Fragments arranged by size:
4169 1210 664
BclI T'GATC_A
Cuts at: 1548 2073 1548
Size: 525 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2248 2397 4654
Size: 107 122 71 631 605 576 149 2257
Cuts at: 4654 136
Size: 1525
Fragments arranged by size:
2257 1525 631 605 576 149 122 107
71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 3460 3460
Size: 6043
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2187 2319 2450 2574 2781 2854 2865
Size: 232 165 132 131 124 207 73 11
Cuts at: 2865 2964 4104 5260 1790
Size: 99 1140 1156 2573
Fragments arranged by size:
2573 1156 1140 232 207 165 132 131
124 99 73 11
BmgBI CAC'GTC
Cuts at: 1318 2359 1318
Size: 1041 5002
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3774 4732
Size: 416 53 83 399 24 897 1460 958
Cuts at: 4732 442
Size: 1753
Fragments arranged by size:
1753 1460 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3175 3175
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 4703 1239
Size: 917 2547 2579
Fragments arranged by size:
2579 2547 917
Bpu10I CC'TnA_GC
Cuts at: 2437 2437
Size: 6043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 4706 882
Size: 617 1092 2115 2219
Fragments arranged by size:
2219 2115 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 3834 493
Size: 711 909 1721 2702
Fragments arranged by size:
2702 1721 909 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2889 3850 4232 4867
Size: 53 83 186 936 1356 961 382 635
Cuts at: 4867 275
Size: 1451
Fragments arranged by size:
1451 1356 961 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4476 4874 5148 5295 5706 4476
Size: 398 274 147 411 4813
Fragments arranged by size:
4813 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2097 2127 1178
Size: 30 165 30 694 30 5094
Fragments arranged by size:
5094 694 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 3344 1186
Size: 1303 855 3885
Fragments arranged by size:
3885 1303 855
BseYI C'CCAG_C
Cuts at: 2419 2874 5246 5742 2419
Size: 455 2372 496 2720
Fragments arranged by size:
2720 2372 496 455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2313 2777 3173 1163
Size: 573 577 464 396 4033
Fragments arranged by size:
4033 577 573 464 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 3493 4254 5282 1540
Size: 275 186 102 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 2022 2088 2382 2574 2871 4104 4189 5260 2022
Size: 66 294 192 297 1233 85 1071 2805
Fragments arranged by size:
2805 1233 1071 297 294 192 85 66
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
BsmBI CGTCTCn'nnnn_
Cuts at: 2175 2175
Size: 6043
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2088 2187 2319 2382 2438 2450
Size: 399 232 66 99 132 63 56 12
Cuts at: 2450 2550 2574 2781 2854 2865 2871 2964 3362
Size: 100 24 207 73 11 6 93 398
Cuts at: 3362 4104 4189 5260 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 232 207 132
100 99 93 85 73 66 63 56
24 12 11 6
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 2493 3932 5662 2493
Size: 1439 1730 2874
Fragments arranged by size:
2874 1730 1439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2760 3398 844
Size: 1916 638 3489
Fragments arranged by size:
3489 1916 638
BsrBI CCG'CTC
Cuts at: 1394 1823 2821 3930 1394
Size: 429 998 1109 3507
Fragments arranged by size:
3507 1109 998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 4874 1700
Size: 110 2877 187 2869
Fragments arranged by size:
2877 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 2710 1184
Size: 1526 4517
BstYI r'GATC_y
Cuts at: 1972 2466 3373 3740 4125 4142 5594 5680 5692
Size: 494 907 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 907 494 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2220 2830 2923 2940 2956 3079 513
Size: 552 1155 610 93 17 16 123 3477
Fragments arranged by size:
3477 1155 610 552 123 93 17 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 4436
Fragments arranged by size:
4436 868 683 56
BtsI GCAGTG_nn'
Cuts at: 2355 2380 3559 4353 4373 2355
Size: 25 1179 794 20 4025
Fragments arranged by size:
4025 1179 794 25 20
ClaI AT'CG_AT
Cuts at: 1554 3336 1554
Size: 1782 4261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DraIII CAC_nnn'GTG
Cuts at: 2689 2689
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2281 2904
Size: 54 1425 50 275 18 270 181 623
Cuts at: 2904 2926 2943 2997 3057 3120 3490 4379 6008
Size: 22 17 54 60 63 370 889 1629
Cuts at: 6008 8
Size: 43
Fragments arranged by size:
1629 1425 889 623 370 275 270 181
63 60 54 54 50 43 22 18
17
EagI C'GGCC_G
Cuts at: 1537 1812 2100 3490 1537
Size: 275 288 1390 4090
Fragments arranged by size:
4090 1390 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3321 3973 1353
Size: 1968 652 3423
Fragments arranged by size:
3423 1968 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2683 3251 4630 5004 5150 387
Size: 1064 138 1094 568 1379 374 146 1280
Fragments arranged by size:
1379 1280 1094 1064 568 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 2869
Size: 6043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2488 2669 3107 4106 4703 5490
Size: 393 524 332 181 438 999 597 787
Cuts at: 5490 5758 1239
Size: 268 1524
Fragments arranged by size:
1524 999 787 597 524 438 393 332
268 181
EcoO109I rG'GnC_Cy
Cuts at: 2674 2851 2674
Size: 177 5866
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FspI TGC'GCA
Cuts at: 2756 4549 2756
Size: 1793 4250
HaeII r_GCGC'y
Cuts at: 1678 2129 2624 5190 1678
Size: 451 495 2566 2531
Fragments arranged by size:
2566 2531 495 451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 3245 3277 4684
Size: 32 135 32 581 32 1060 32 1407
Cuts at: 4684 4716 4758 4790 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1407 1060 581 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3309 748
Size: 2561 3482
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 5157 5341 1305
Size: 1206 2646 184 2007
Fragments arranged by size:
2646 2007 1206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2283 2945 2999 10
Size: 54 1425 343 451 662 54 3054
Fragments arranged by size:
3054 1425 662 451 343 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 2969 4001 4360 4519 518
Size: 648 1374 429 1032 359 159 2042
Fragments arranged by size:
2042 1374 1032 648 429 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2578 2832 2958 3081 3459 4136 5284
Size: 528 444 254 126 123 378 677 1148
Cuts at: 5284 5529 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 528 444 378 254 245
126 123
NcoI C'CATG_G
Cuts at: 513 1065 2220 2923 513
Size: 552 1155 703 3633
Fragments arranged by size:
3633 1155 703 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3401 4946 1132
Size: 97 2172 1545 2229
Fragments arranged by size:
2229 2172 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2187 1348
Size: 839 5204
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 2581 1108
Size: 956 517 4570
Fragments arranged by size:
4570 956 517
PfoI T'CCnGG_A
Cuts at: 2481 3126 2481
Size: 645 5398
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2674 2674
Size: 6043
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PspOMI G'GGCC_C
Cuts at: 2850 2960 2850
Size: 110 5933
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 6011
PstI C_TGCA'G
Cuts at: 830 2133 2397 3395 830
Size: 1303 264 998 3478
Fragments arranged by size:
3478 1303 998 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2134 2578 2134
Size: 444 5599
RsrII CG'GwC_CG
Cuts at: 2485 2485
Size: 6043
SacI G_AGCT'C
Cuts at: 2871 2871
Size: 6043
SacII CC_GC'GG
Cuts at: 2833 2959 3082 2833
Size: 126 123 5794
Fragments arranged by size:
5794 126 123
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SbfI CC_TGCA'GG
Cuts at: 2397 3395 2397
Size: 998 5045
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SexAI A'CCwGG_T
Cuts at: 2685 3255 2685
Size: 570 5473
SfcI C'TryA_G
Cuts at: 826 1045 2129 2393 3391 4526 5207 5398 826
Size: 219 1084 264 998 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 998 681 264 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 2733 3370 1872
Size: 861 637 4545
Fragments arranged by size:
4545 861 637
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 3182 1517
Size: 1665 4378
StyI C'CwwG_G
Cuts at: 513 1065 2220 2523 2923 3482 513
Size: 552 1155 303 400 559 3074
Fragments arranged by size:
3074 1155 559 552 400 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2888 4070 4087 4240 4425 4870 325
Size: 1255 1308 1182 17 153 185 445 1498
Fragments arranged by size:
1498 1308 1255 1182 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4289 6001 96
Size: 274 80 33 51 1443 2312 1712 138
Fragments arranged by size:
2312 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2675 2707 6003 6035 2097
Size: 32 546 32 3296 32 2105
Fragments arranged by size:
3296 2105 546 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2899 2977 2194
Size: 705 78 5260
Fragments arranged by size:
5260 705 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 2731 3368 1870
Size: 861 637 4545
Fragments arranged by size:
4545 861 637
XmnI GAAnn'nnTTC
Cuts at: 2501 4172 2501
Size: 1671 4372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI SanDI SapI SfiI SfoI SrfI
SwaI