Restriction map for
pFN21AE1526
(Circular) (Six-base) MAPSORT of: pFN21AE1526.seq Check: 8396 from: 1 to: 5791
pFN21AE1526 5791 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2463 2548 2728 2463
Size: 85 180 5526
Fragments arranged by size:
5526 180 85
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 2469 3112 2469
Size: 643 5148
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2058 2205 3854 5238 5506 1632
Size: 426 147 1649 1384 268 1917
Fragments arranged by size:
1917 1649 1384 426 268 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2449 2471 1184
Size: 1265 22 4504
Fragments arranged by size:
4504 1265 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 5759
AlwNI CAG_nnn'CTG
Cuts at: 2236 2563 2782 2842 2995 3534 5106 2236
Size: 327 219 60 153 539 1572 2921
Fragments arranged by size:
2921 1572 539 327 219 153 60
ApaI G_GGCC'C
Cuts at: 2383 2383
Size: 5791
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 3116 1870
Size: 73 176 997 4545
Fragments arranged by size:
4545 997 176 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2469 2573 3112 4567 618
Size: 325 217 627 682 104 539 1455 1842
Fragments arranged by size:
1842 1455 682 627 539 325 217 104
BanII G_rGCy'C
Cuts at: 1391 2120 2183 2383 2899 2973 3110 1391
Size: 729 63 200 516 74 137 4072
Fragments arranged by size:
4072 729 516 200 137 74 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 2351 2703
Size: 107 122 71 631 605 580 99 352
Cuts at: 2703 4402 136
Size: 1699 1525
Fragments arranged by size:
1699 1525 631 605 580 352 122 107
99 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2383 2578 3852 5008 1790
Size: 593 195 1274 1156 2573
Fragments arranged by size:
2573 1274 1156 593 195
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2966 3522 4480
Size: 416 53 83 399 24 1549 556 958
Cuts at: 4480 442
Size: 1753
Fragments arranged by size:
1753 1549 958 556 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 2405 2495 2984 3046 3047 4451 1239
Size: 948 218 90 489 62 1 1404 2579
Fragments arranged by size:
2579 1404 948 489 218 90 62 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2005 2735 3916 4781 5079 2005
Size: 730 1181 865 298 2717
Fragments arranged by size:
2717 1181 865 730 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4454 882
Size: 617 2955 2219
Fragments arranged by size:
2955 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2526 2887 3582 493
Size: 711 1322 361 695 2702
Fragments arranged by size:
2702 1322 711 695 361
BsaBI GATnn'nnATC
Cuts at: 2168 5785 2168
Size: 3617 2174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2227 2610 3598 3980
Size: 53 83 186 936 694 383 988 382
Cuts at: 3980 4615 275
Size: 635 1451
Fragments arranged by size:
1451 988 936 694 635 383 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2130 2160 2234 2264 3014
Size: 30 165 30 727 30 74 30 750
Cuts at: 3014 3044 1178
Size: 30 3925
Fragments arranged by size:
3925 750 727 165 74 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2190 2234 2291 2963 2981 3023 3035
Size: 909 95 44 57 672 18 42 12
Cuts at: 3035 1186
Size: 3942
Fragments arranged by size:
3942 909 672 95 57 44 42 18
12
BseYI C'CCAG_C
Cuts at: 2111 2336 2563 2675 3067 4994 5490 2111
Size: 225 227 112 392 1927 496 2412
Fragments arranged by size:
2412 1927 496 392 227 225 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2548 2762 2923 1163
Size: 573 812 214 161 4031
Fragments arranged by size:
4031 812 573 214 161
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2358 3241 4002 5030 1540
Size: 275 186 357 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 357 275 186
BsiHKAI G_wGCw'C
Cuts at: 2822 2868 2886 2973 3852 3937 5008 2822
Size: 46 18 87 879 85 1071 3605
Fragments arranged by size:
3605 1071 879 87 85 46 18
BsmI GAATG_Cn'
Cuts at: 2765 3302 3395 2765
Size: 537 93 5161
Fragments arranged by size:
5161 537 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2183 2383 2578 2822 2868 2886
Size: 399 330 63 200 195 244 46 18
Cuts at: 2886 2899 2973 3110 3852 3937 5008 1391
Size: 13 74 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 330 244 200 195
137 85 74 63 46 18 13
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2463 2548 2728 2790 3146 844
Size: 1619 85 180 62 356 3489
Fragments arranged by size:
3489 1619 356 180 85 62
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2466 4435 4622 1700
Size: 110 656 1969 187 2869
Fragments arranged by size:
2869 1969 656 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 2879 3851 4863 2879
Size: 972 1012 3807
Fragments arranged by size:
3807 1012 972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2740 2782 2932 794
Size: 1946 42 150 3653
Fragments arranged by size:
3653 1946 150 42
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2444 2830 2444
Size: 386 5405
BstYI r'GATC_y
Cuts at: 1972 3121 3488 3873 3890 5342 5428 5440 5786
Size: 1149 367 385 17 1452 86 12 346
Cuts at: 5786 1972
Size: 1977
Fragments arranged by size:
1977 1452 1149 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2175 2823 513
Size: 552 1110 648 3481
Fragments arranged by size:
3481 1110 648 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 2331 3307 4101 4121 2331
Size: 976 794 20 4001
Fragments arranged by size:
4001 976 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2355 2394
Size: 54 1425 50 275 18 336 189 39
Cuts at: 2394 3238 4127 5756 8
Size: 844 889 1629 43
Fragments arranged by size:
1629 1425 889 844 336 275 189 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2355 3238 1537
Size: 275 543 883 4090
Fragments arranged by size:
4090 883 543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 2181 2288 2645 3721 1353
Size: 724 104 107 357 1076 3423
Fragments arranged by size:
3423 1076 724 357 107 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2684 2966 4378 4752 4898 387
Size: 1064 138 1095 282 1412 374 146 1280
Fragments arranged by size:
1412 1280 1095 1064 374 282 146 138
EcoICRI GAG'CTC
Cuts at: 2971 2971
Size: 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2187 2205 2405 2495 2984 3046
Size: 393 426 129 18 200 90 489 62
Cuts at: 3046 3047 3854 4451 5238 5506 1239
Size: 1 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 489 426 393 268
200 129 90 62 18 1
EcoO109I rG'GnC_Cy
Cuts at: 2379 2380 2635 2811 2379
Size: 1 255 176 5359
Fragments arranged by size:
5359 255 176 1
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 5791
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2048 4938 1678
Size: 370 2890 2531
Fragments arranged by size:
2890 2531 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2234 2266 2693
Size: 32 135 32 431 32 199 32 427
Cuts at: 2693 2725 4432 4464 4506 4538 1373
Size: 32 1707 32 42 32 2626
Fragments arranged by size:
2626 1707 431 427 199 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 2473 3116 2473
Size: 643 5148
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 10
Size: 54 1425 343 336 3633
Fragments arranged by size:
3633 1425 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 3749 4108 4267 518
Size: 648 1662 921 359 159 2042
Fragments arranged by size:
2042 1662 921 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2272 2440 2842 2985 2992 3071 3207
Size: 571 95 168 402 143 7 79 136
Cuts at: 3207 3884 5032 5277 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 571 402 245 168 143
136 95 79 7
NcoI C'CATG_G
Cuts at: 513 1065 2823 513
Size: 552 1758 3481
Fragments arranged by size:
3481 1758 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2452 3149 4694 1132
Size: 97 1223 697 1545 2229
Fragments arranged by size:
2229 1545 1223 697 97
PasI CC'CwG_GG
Cuts at: 1348 2062 1348
Size: 714 5077
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 1108
Size: 1734 4057
PfoI T'CCnGG_A
Cuts at: 2550 2550
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PmlI CAC'GTG
Cuts at: 2526 2887 2526
Size: 361 5430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3860 3892 5373 5405 3014
Size: 32 814 32 1481 32 3400
Fragments arranged by size:
3400 1481 814 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2811 2811
Size: 5791
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PspOMI G'GGCC_C
Cuts at: 2379 2379
Size: 5791
PstI C_TGCA'G
Cuts at: 830 2163 2241 2688 2778 3000 3143 830
Size: 1333 78 447 90 222 143 3478
Fragments arranged by size:
3478 1333 447 222 143 90 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
PvuII CAG'CTG
Cuts at: 2440 2842 2992 2440
Size: 402 150 5239
Fragments arranged by size:
5239 402 150
SacI G_AGCT'C
Cuts at: 2973 2973
Size: 5791
SacII CC_GC'GG
Cuts at: 2178 2178
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SanDI GG'GwC_CC
Cuts at: 2811 2811
Size: 5791
SapI GCTCTTCn'nnn_
Cuts at: 2077 2181 2288 2077
Size: 104 107 5580
Fragments arranged by size:
5580 107 104
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 2159 2237 2684 2774 2996 3047 3139
Size: 219 1114 78 447 90 222 51 92
Cuts at: 3139 4274 4955 5146 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1114 681 447 222 219 191
92 90 78 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2020 2750 3895 4796 5058 820
Size: 197 926 77 730 1145 901 262 1553
Fragments arranged by size:
1553 1145 926 901 730 262 197 77
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 2452 3149 1132
Size: 1320 697 3774
Fragments arranged by size:
3774 1320 697
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5791
StyI C'CwwG_G
Cuts at: 513 1065 2149 2823 3230 513
Size: 552 1084 674 407 3074
Fragments arranged by size:
3074 1084 674 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2561 3818 3835 3988 4173 4618 325
Size: 1255 981 1257 17 153 185 445 1498
Fragments arranged by size:
1498 1257 1255 981 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4037 5749 96
Size: 274 80 33 51 1443 2060 1712 138
Fragments arranged by size:
2060 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2130 2162 5751 5783 2130
Size: 32 3589 32 2138
Fragments arranged by size:
3589 2138 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2653 2653
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SexAI SfiI SfoI SrfI
SwaI