Restriction map for
pFN21AE1524
(Circular) (Six-base) MAPSORT of: pFN21AE1524.seq Check: 8062 from: 1 to: 6688
pFN21AE1524 6688 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4498 278
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2232 4031 1937
Size: 295 1799 4594
Fragments arranged by size:
4594 1799 295
Acc65I G'GTAC_C
Cuts at: 4009 4009
Size: 6688
AclI AA'CG_TT
Cuts at: 1563 4815 5188 1563
Size: 3252 373 3063
Fragments arranged by size:
3252 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 4751 6135 6403 1632
Size: 425 2694 1384 268 1917
Fragments arranged by size:
2694 1917 1384 425 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6491
AflIII A'CryG_T
Cuts at: 1203 2539 2919 5587 6564 1203
Size: 1336 380 2668 977 1327
Fragments arranged by size:
2668 1336 1327 977 380
AgeI A'CCGG_T
Cuts at: 5519 5519
Size: 6688
AhdI GACnn_n'nnGTC
Cuts at: 5417 5417
Size: 6688
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2774 1184
Size: 1590 5098
AlwNI CAG_nnn'CTG
Cuts at: 2441 2578 3010 4431 6003 2441
Size: 137 432 1421 1572 3126
Fragments arranged by size:
3126 1572 1421 432 137
ApaI G_GGCC'C
Cuts at: 2719 2850 3058 3088 2719
Size: 131 208 30 6319
Fragments arranged by size:
6319 208 131 30
ApaLI G'TGCA_C
Cuts at: 2393 2729 4745 5901 2393
Size: 336 2016 1156 3180
Fragments arranged by size:
3180 2016 1156 336
ApoI r'AATT_y
Cuts at: 1493 3555 3997 4223 4375 6484 1493
Size: 2062 442 226 152 2109 1697
Fragments arranged by size:
2109 2062 1697 442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5242 160
Size: 872 4210 1606
Fragments arranged by size:
4210 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
AvaI C'yCGr_G
Cuts at: 1870 1943 2823 3369 4013 1870
Size: 73 880 546 644 4545
Fragments arranged by size:
4545 880 644 546 73
AvrII C'CTAG_G
Cuts at: 3032 3032
Size: 6688
BamHI G'GATC_C
Cuts at: 4018 4385 4018
Size: 367 6321
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 2599 2819 3188 3359
Size: 325 217 627 511 301 220 369 171
Cuts at: 3359 4009 5464 618
Size: 650 1455 1842
Fragments arranged by size:
1842 1455 650 627 511 369 325 301
220 217 171
BanII G_rGCy'C
Cuts at: 1391 2274 2612 2719 2850 3025 3058 3088 3715
Size: 883 338 107 131 175 33 30 627
Cuts at: 3715 4007 1391
Size: 292 4072
Fragments arranged by size:
4072 883 627 338 292 175 131 107
33 30
BbeI G_GCGC'C
Cuts at: 2603 3192 2603
Size: 589 6099
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5717
BbvCI CC'TCA_GC
Cuts at: 3630 3630
Size: 6688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4879 4913 1903
Size: 34 50 34 2858 34 3678
Fragments arranged by size:
3678 2858 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4580 5790 4580
Size: 1210 5478
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6688
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2478 5299 136
Size: 107 122 71 631 605 806 2821 1525
Fragments arranged by size:
2821 1525 806 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6683 6683
Size: 6688
BlpI GC'TnA_GC
Cuts at: 4105 4105
Size: 6688
Bme1580I G_kGCm'C
Cuts at: 1790 2397 2483 2719 2733 2824 2850 2878 3058
Size: 607 86 236 14 91 26 28 180
Cuts at: 3058 3088 3579 4749 5905 1790
Size: 30 491 1170 1156 2573
Fragments arranged by size:
2573 1170 1156 607 491 236 180 91
86 30 28 26 14
BmgBI CAC'GTC
Cuts at: 1318 2920 1318
Size: 1602 5086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2059 2236 2412
Size: 416 53 83 399 24 642 177 176
Cuts at: 2412 2429 2498 2576 2615 3849 4419 5377 442
Size: 17 69 78 39 1234 570 958 1753
Fragments arranged by size:
1753 1234 958 642 570 416 399 177
176 83 78 69 53 39 24 17
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2971 2038
Size: 933 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3032 3034 3143 3488 3518 3573 3729 3756
Size: 1793 2 109 345 30 55 156 27
Cuts at: 3756 3953 5348 1239
Size: 197 1395 2579
Fragments arranged by size:
2579 1793 1395 345 197 156 109 55
30 27 2
Bpu10I CC'TnA_GC
Cuts at: 2259 2307 3630 2259
Size: 48 1323 5317
Fragments arranged by size:
5317 1323 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2309 3857 4813 5678 5976 2105
Size: 204 1548 956 865 298 2817
Fragments arranged by size:
2817 1548 956 865 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2730 5351 882
Size: 617 1231 2621 2219
Fragments arranged by size:
2621 2219 1231 617
BsaAI yAC'GTr
Cuts at: 493 1204 2542 4479 493
Size: 711 1338 1937 2702
Fragments arranged by size:
2702 1937 1338 711
BsaBI GATnn'nnATC
Cuts at: 6682 6682
Size: 6688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2600 2765 3189 4495
Size: 53 83 186 936 1067 165 424 1306
Cuts at: 4495 4877 5512 275
Size: 382 635 1451
Fragments arranged by size:
1451 1306 1067 936 635 424 382 186
165 83 53
BsaWI w'CCGG_w
Cuts at: 3864 5121 5519 5793 5940 6351 3864
Size: 1257 398 274 147 411 4201
Fragments arranged by size:
4201 1257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2286 2316 3837 3867 1178
Size: 30 165 30 883 30 1521 30 3999
Fragments arranged by size:
3999 1521 883 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 3466 3618 1186
Size: 916 1364 152 4256
Fragments arranged by size:
4256 1364 916 152
BseYI C'CCAG_C
Cuts at: 2169 2368 3081 3295 3436 3635 5891 6387 2169
Size: 199 713 214 141 199 2256 496 2470
Fragments arranged by size:
2470 2256 713 496 214 199 199 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 2600 1163
Size: 573 801 63 5251
Fragments arranged by size:
5251 801 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 4138 4899 5927 1540
Size: 275 186 1102 1035 761 1028 2301
Fragments arranged by size:
2301 1102 1035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2265 2397 2535 2733 4749 4834 5905 2115
Size: 150 132 138 198 2016 85 1071 2898
Fragments arranged by size:
2898 2016 1071 198 150 138 132 85
BsmI GAATG_Cn'
Cuts at: 3708 4199 4292 3708
Size: 491 93 6104
Fragments arranged by size:
6104 491 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2265 2274 2397 2483 2535 2612
Size: 399 325 150 9 123 86 52 77
Cuts at: 2612 2719 2733 2824 2850 2878 3025 3058 3088
Size: 107 14 91 26 28 147 33 30
Cuts at: 3088 3579 3715 4007 4749 4834 5905 1391
Size: 491 136 292 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 491 399 325 292 150
147 136 123 107 91 86 85 77
52 33 30 28 26 14 9
BspEI T'CCGG_A
Cuts at: 3864 6351 3864
Size: 2487 4201
BspHI T'CATG_A
Cuts at: 2517 4577 6307 2517
Size: 2060 1730 2898
Fragments arranged by size:
2898 2060 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4043 844
Size: 3199 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4575 1394
Size: 429 2752 3507
Fragments arranged by size:
3507 2752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5183 5357 65
Size: 1183 3935 174 1396
Fragments arranged by size:
3935 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2160 3102 3903 5332 5519 1700
Size: 110 350 942 801 1429 187 2869
Fragments arranged by size:
2869 1429 942 801 350 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6688
BssHII G'CGCG_C
Cuts at: 1925 2621 1925
Size: 696 5992
BssSI C'ACGA_G
Cuts at: 2657 4748 5760 2657
Size: 2091 1012 3585
Fragments arranged by size:
3585 2091 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6688
BstBI TT'CG_AA
Cuts at: 5492 5492
Size: 6688
BstEII G'GTnAC_C
Cuts at: 1184 3209 3802 1184
Size: 2025 593 4070
Fragments arranged by size:
4070 2025 593
BstXI CCAn_nnnn'nTGG
Cuts at: 2785 3496 2785
Size: 711 5977
BstYI r'GATC_y
Cuts at: 1972 2238 4018 4385 4770 4787 6239 6325 6337
Size: 266 1780 367 385 17 1452 86 12
Cuts at: 6337 6683 1972
Size: 346 1977
Fragments arranged by size:
1977 1780 1452 385 367 346 266 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2013 2133 3194 2013
Size: 120 1061 5507
Fragments arranged by size:
5507 1061 120
BtgI C'CryG_G
Cuts at: 513 1065 2294 2700 2719 2893 3696 513
Size: 552 1229 406 19 174 803 3505
Fragments arranged by size:
3505 1229 803 552 406 174 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5949
Fragments arranged by size:
5949 683 56
BtsI GCAGTG_nn'
Cuts at: 3312 4204 4998 5018 3312
Size: 892 794 20 4982
Fragments arranged by size:
4982 892 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6653
DraI TTT'AAA
Cuts at: 3992 3992
Size: 6688
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5695 809
Size: 507 4379 1802
Fragments arranged by size:
4379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2105 3978 4135
Size: 54 1425 50 275 18 275 1873 157
Cuts at: 4135 5024 6653 8
Size: 889 1629 43
Fragments arranged by size:
1873 1629 1425 889 275 275 157 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4135 1537
Size: 275 2323 4090
Fragments arranged by size:
4090 2323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2681 3893 4618 1353
Size: 1328 1212 725 3423
Fragments arranged by size:
3423 1328 1212 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5275 5649 5795 387
Size: 1064 138 3686 374 146 1280
Fragments arranged by size:
3686 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 3032 3034 3143 3488 3518 3573
Size: 393 425 975 2 109 345 30 55
Cuts at: 3573 3729 3756 3953 4751 5348 6135 6403 1239
Size: 156 27 197 798 597 787 268 1524
Fragments arranged by size:
1524 975 798 787 597 425 393 345
268 197 156 109 55 30 27 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 2504
Size: 6688
EcoO109I rG'GnC_Cy
Cuts at: 2137 2320 2462 2715 2847 2983 3054 3055 3085
Size: 183 142 253 132 136 71 1 30
Cuts at: 3085 3584 3949 2137
Size: 499 365 4876
Fragments arranged by size:
4876 499 365 253 183 142 136 132
71 30 1
EcoRI G'AATT_C
Cuts at: 3997 4375 3997
Size: 378 6310
FseI GG_CCGG'CC
Cuts at: 2164 2164
Size: 6688
FspI TGC'GCA
Cuts at: 5194 5194
Size: 6688
HaeII r_GCGC'y
Cuts at: 1678 2603 3192 5835 1678
Size: 925 589 2643 2531
Fragments arranged by size:
2643 2531 925 589
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5329 5361 5403 5435 1373
Size: 32 135 32 3757 32 42 32 2626
Fragments arranged by size:
3757 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4032 4278 669
Size: 988 281 2094 246 3079
Fragments arranged by size:
3079 2094 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6688
HpaI GTT'AAC
Cuts at: 4278 4278
Size: 6688
KasI G'GCGC_C
Cuts at: 2599 3188 2599
Size: 589 6099
KpnI G_GTAC'C
Cuts at: 4013 4013
Size: 6688
MfeI C'AATT_G
Cuts at: 4287 4287
Size: 6688
MluI A'CGCG_T
Cuts at: 6564 6564
Size: 6688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5802 5986 1305
Size: 4497 184 2007
Fragments arranged by size:
4497 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3980 10
Size: 54 1425 343 2148 2718
Fragments arranged by size:
2718 2148 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2783 3701 4646 5005 5164 518
Size: 648 987 630 918 945 359 159 2042
Fragments arranged by size:
2042 987 945 918 648 630 359 159
MspA1I CmG'CkG
Cuts at: 1606 2653 2689 2721 3007 3725 3959 4104 4781
Size: 1047 36 32 286 718 234 145 677
Cuts at: 4781 5929 6174 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1047 718 677 286 245 234
145 36 32
NaeI GCC'GGC
Cuts at: 2162 2162
Size: 6688
NarI GG'CG_CC
Cuts at: 2600 3189 2600
Size: 589 6099
NcoI C'CATG_G
Cuts at: 513 1065 2700 3696 513
Size: 552 1635 996 3505
Fragments arranged by size:
3505 1635 996 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6688
NgoMIV G'CCGG_C
Cuts at: 2160 2160
Size: 6688
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6688
NotI GC'GGCC_GC
Cuts at: 4135 4135
Size: 6688
NspI r_CATG'y
Cuts at: 1132 1229 3162 3463 4046 5591 1132
Size: 97 1933 301 583 1545 2229
Fragments arranged by size:
2229 1933 1545 583 301 97
PasI CC'CwG_GG
Cuts at: 1348 2973 3580 1348
Size: 1625 607 4456
Fragments arranged by size:
4456 1625 607
PciI A'CATG_T
Cuts at: 5587 5587
Size: 6688
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3252 1108
Size: 2144 4544
PfoI T'CCnGG_A
Cuts at: 2046 3488 3930 2046
Size: 1442 442 4804
Fragments arranged by size:
4804 1442 442
PmeI GTTT'AAAC
Cuts at: 3992 3992
Size: 6688
PmlI CAC'GTG
Cuts at: 2542 2542
Size: 6688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4789 6270 6302 4757
Size: 32 1481 32 5143
Fragments arranged by size:
5143 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2462 2983 3584 3949 2137
Size: 325 521 601 365 4876
Fragments arranged by size:
4876 601 521 365 325
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6688
PsiI TTA'TAA
Cuts at: 4258 4258
Size: 6688
PspOMI G'GGCC_C
Cuts at: 2715 2846 3054 3084 2715
Size: 131 208 30 6319
Fragments arranged by size:
6319 208 131 30
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2216 2248 2216
Size: 32 6656
PstI C_TGCA'G
Cuts at: 830 2583 3006 4040 830
Size: 1753 423 1034 3478
Fragments arranged by size:
3478 1753 1034 423
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6688
PvuII CAG'CTG
Cuts at: 2689 3959 2689
Size: 1270 5418
SacII CC_GC'GG
Cuts at: 2722 2722
Size: 6688
SalI G'TCGA_C
Cuts at: 1936 4030 1936
Size: 2094 4594
SanDI GG'GwC_CC
Cuts at: 2983 2983
Size: 6688
SapI GCTCTTCn'nnn_
Cuts at: 3893 3893
Size: 6688
SbfI CC_TGCA'GG
Cuts at: 4040 4040
Size: 6688
ScaI AGT'ACT
Cuts at: 4936 6648 4936
Size: 1712 4976
SfcI C'TryA_G
Cuts at: 826 1045 2579 3002 4036 5171 5852 6043 826
Size: 219 1534 423 1034 1135 681 191 1471
Fragments arranged by size:
1534 1471 1135 1034 681 423 219 191
SfoI GGC'GCC
Cuts at: 2601 3190 2601
Size: 589 6099
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6688
SmaI CCC'GGG
Cuts at: 1872 2825 3371 4015 1872
Size: 953 546 644 4545
Fragments arranged by size:
4545 953 644 546
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 2324 3872 4792 5693 5955
Size: 197 926 141 240 1548 920 901 262
Cuts at: 5955 820
Size: 1553
Fragments arranged by size:
1553 1548 926 920 901 262 240 197
141
SnaBI TAC'GTA
Cuts at: 493 4479 493
Size: 3986 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6688
SphI G_CATG'C
Cuts at: 1132 4046 1132
Size: 2914 3774
SspI AAT'ATT
Cuts at: 5 52 4612 5
Size: 47 4560 2081
Fragments arranged by size:
4560 2081 47
StuI AGG'CCT
Cuts at: 1517 3037 3594 3741 1517
Size: 1520 557 147 4464
Fragments arranged by size:
4464 1520 557 147
StyI C'CwwG_G
Cuts at: 513 1065 2242 2700 3032 3696 4127 513
Size: 552 1177 458 332 664 431 3074
Fragments arranged by size:
3074 1177 664 552 458 431 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2503 2550 3399 3506 3821 4715 4732
Size: 1255 923 47 849 107 315 894 17
Cuts at: 4732 4885 5070 5515 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 923 894 849 445 315 185
153 107 47 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2563 4934 6646
Size: 274 80 33 51 1443 586 2371 1712
Cuts at: 6646 96
Size: 138
Fragments arranged by size:
2371 1712 1443 586 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 6648 6680 2286
Size: 32 4330 32 2294
Fragments arranged by size:
4330 2294 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6688
XbaI T'CTAG_A
Cuts at: 4024 4024
Size: 6688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 2707
Size: 6688
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6688
XmaI C'CCGG_G
Cuts at: 1870 2823 3369 4013 1870
Size: 953 546 644 4545
Fragments arranged by size:
4545 953 644 546
XmnI GAAnn'nnTTC
Cuts at: 4817 4817
Size: 6688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4496 276
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI
PacI PspXI RsrII SacI SexAI SfiI SrfI SwaI