Restriction map for
pFN21AE1521
(Circular) (Six-base) MAPSORT of: pFN21AE1521.seq Check: 8664 from: 1 to: 5692
pFN21AE1521 5692 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 3035 1937
Size: 271 827 4594
Fragments arranged by size:
4594 827 271
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2288 2395 3755 5139 5407 1632
Size: 470 186 107 1360 1384 268 1917
Fragments arranged by size:
1917 1384 1360 470 268 186 107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 2984 4591 5568 1203
Size: 1781 1607 977 1327
Fragments arranged by size:
1781 1607 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaLI G'TGCA_C
Cuts at: 2017 3749 4905 2017
Size: 1732 1156 2804
Fragments arranged by size:
2804 1732 1156
ApoI r'AATT_y
Cuts at: 1493 2225 2383 2464 2533 3001 3227 3379 5488
Size: 732 158 81 69 468 226 152 2109
Cuts at: 5488 1493
Size: 1697
Fragments arranged by size:
2109 1697 732 468 226 158 152 81
69
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 2008 3022 3389 2008
Size: 1014 367 4311
Fragments arranged by size:
4311 1014 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3013 4468 618
Size: 325 217 627 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3011 1391
Size: 1620 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2963 928
Size: 971 1064 3657
Fragments arranged by size:
3657 1064 971
BbvCI CC'TCA_GC
Cuts at: 2721 2721
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2921 5687 2921
Size: 2766 2926
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2021 3753 4909 1790
Size: 231 1732 1156 2573
Fragments arranged by size:
2573 1732 1156 231
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2008 3423 4381
Size: 416 53 83 399 24 591 1415 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1415 958 591 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4352 1239
Size: 3113 2579
Bpu10I CC'TnA_GC
Cuts at: 2721 2721
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2556 2732 3817 4682 4980 2556
Size: 176 1085 865 298 3268
Fragments arranged by size:
3268 1085 865 298 176
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 2369 5686 2369
Size: 3317 2375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2067 1186
Size: 881 4811
BseYI C'CCAG_C
Cuts at: 2934 4895 5391 2934
Size: 1961 496 3235
Fragments arranged by size:
3235 1961 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 3753 3838 4909 2021
Size: 1732 85 1071 2804
Fragments arranged by size:
2804 1732 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 3011 3753 3838 4909 1391
Size: 399 231 990 742 85 1071 2174
Fragments arranged by size:
2174 1071 990 742 399 231 85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2480 3047 844
Size: 1280 356 567 3489
Fragments arranged by size:
3489 1280 567 356
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 2457 96
Size: 2361 3331
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 2954 4496 2954
Size: 1542 4150
BstEII G'GTnAC_C
Cuts at: 1184 2489 1184
Size: 1305 4387
BstXI CCAn_nnnn'nTGG
Cuts at: 2251 2863 2251
Size: 612 5080
BstYI r'GATC_y
Cuts at: 1972 2008 2921 3022 3389 3774 3791 5243 5329
Size: 36 913 101 367 385 17 1452 86
Cuts at: 5329 5341 5687 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 913 385 367 346 101 86
36 17 12
BstZ17I GTA'TAC
Cuts at: 2209 2209
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2726 2761 529
Size: 35 2162 35 3460
Fragments arranged by size:
3460 2162 35 35
DraI TTT'AAA
Cuts at: 2947 2996 2947
Size: 49 5643
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657
Size: 54 1425 50 275 18 1309 889 1629
Cuts at: 5657 8
Size: 43
Fragments arranged by size:
1629 1425 1309 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2868 3622 1353
Size: 1515 754 3423
Fragments arranged by size:
3423 1515 754
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2288 2395 3755 4352 5139 5407
Size: 393 470 186 107 1360 597 787 268
Cuts at: 5407 1239
Size: 1524
Fragments arranged by size:
1524 1360 787 597 470 393 268 186
107
EcoO109I rG'GnC_Cy
Cuts at: 2716 2716
Size: 5692
EcoRI G'AATT_C
Cuts at: 2383 2533 3001 3379 2383
Size: 150 468 378 4696
Fragments arranged by size:
4696 468 378 150
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2889 2921 4333 4365 4407
Size: 32 135 32 1317 32 1412 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1412 1317 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2417 3036 3282 669
Size: 988 281 479 619 246 3079
Fragments arranged by size:
3079 988 619 479 281 246
HindIII A'AGCT_T
Cuts at: 748 2091 748
Size: 1343 4349
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2261 2789 4806 4990 1305
Size: 956 528 2017 184 2007
Fragments arranged by size:
2017 2007 956 528 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2965 3650 4009 4168 518
Size: 648 1194 605 685 359 159 2042
Fragments arranged by size:
2042 1194 685 648 605 359 159
MspA1I CmG'CkG
Cuts at: 1606 2756 3108 3785 4933 5178 1606
Size: 1150 352 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 677 352 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2988 3050 4595 1132
Size: 97 1759 62 1545 2229
Fragments arranged by size:
2229 1759 1545 97 62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 2984 4591 2984
Size: 1607 4085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2716 2716
Size: 5692
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 2844 3262 2844
Size: 418 5274
PstI C_TGCA'G
Cuts at: 830 2113 3044 830
Size: 1283 931 3478
Fragments arranged by size:
3478 1283 931
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2756 2756
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2109 2703 3040 4175 4856 5047 826
Size: 219 1064 594 337 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 681 594 337 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2571 2711 3796 4697 4959 820
Size: 197 926 628 140 1085 901 262 1553
Fragments arranged by size:
1553 1085 926 901 628 262 197 140
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2744 3131 513
Size: 552 939 740 387 3074
Fragments arranged by size:
3074 939 740 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2457 2758 3938
Size: 274 80 33 51 1443 480 301 1180
Cuts at: 3938 5650 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1180 480 301 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 5652 5684 2000
Size: 32 3620 32 2008
Fragments arranged by size:
3620 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI