Restriction map for
pFN21AE1520
(Circular) (Six-base) MAPSORT of: pFN21AE1520.seq Check: 2202 from: 1 to: 5059
pFN21AE1520 5059 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2304 2304
Size: 5059
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2869 278
Size: 53 83 186 936 1333 2468
Fragments arranged by size:
2468 1333 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2402 1937
Size: 465 4594
Acc65I G'GTAC_C
Cuts at: 2150 2380 2150
Size: 230 4829
AclI AA'CG_TT
Cuts at: 1563 3186 3559 1563
Size: 1623 373 3063
Fragments arranged by size:
3063 1623 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2116 3122 4506 4774 1632
Size: 484 1006 1384 268 1917
Fragments arranged by size:
1917 1384 1006 484 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4862
AflIII A'CryG_T
Cuts at: 1203 3958 4935 1203
Size: 2755 977 1327
Fragments arranged by size:
2755 1327 977
AgeI A'CCGG_T
Cuts at: 3890 3890
Size: 5059
AhdI GACnn_n'nnGTC
Cuts at: 3788 3788
Size: 5059
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5059
AlwNI CAG_nnn'CTG
Cuts at: 2022 2802 4374 2022
Size: 780 1572 2707
Fragments arranged by size:
2707 1572 780
ApaLI G'TGCA_C
Cuts at: 3116 4272 3116
Size: 1156 3903
ApoI r'AATT_y
Cuts at: 1493 2174 2368 2594 2746 4855 1493
Size: 681 194 226 152 2109 1697
Fragments arranged by size:
2109 1697 681 226 194 152
AseI AT'TA_AT
Cuts at: 160 1032 3613 160
Size: 872 2581 1606
Fragments arranged by size:
2581 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5059
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2143 2384 1870
Size: 73 153 47 241 4545
Fragments arranged by size:
4545 241 153 73 47
BamHI G'GATC_C
Cuts at: 2389 2756 2389
Size: 367 4692
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2380 3835 618
Size: 325 217 627 363 230 1455 1842
Fragments arranged by size:
1842 1455 627 363 325 230 217
BanII G_rGCy'C
Cuts at: 1391 2271 2378 1391
Size: 880 107 4072
Fragments arranged by size:
4072 880 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3250 3284 1903
Size: 34 1313 34 3678
Fragments arranged by size:
3678 1313 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2951 4161 2951
Size: 1210 3849
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5059
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3670 136
Size: 107 122 71 631 605 1998 1525
Fragments arranged by size:
1998 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5054 5054
Size: 5059
BlpI GC'TnA_GC
Cuts at: 2476 2476
Size: 5059
Bme1580I G_kGCm'C
Cuts at: 1790 3120 4276 1790
Size: 1330 1156 2573
Fragments arranged by size:
2573 1330 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5059
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2790 3748 442
Size: 416 53 83 399 24 1373 958 1753
Fragments arranged by size:
1753 1373 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3719 1239
Size: 2480 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3184 4049 4347 3184
Size: 865 298 3896
Fragments arranged by size:
3896 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3722 882
Size: 617 2223 2219
Fragments arranged by size:
2223 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2850 493
Size: 711 1646 2702
Fragments arranged by size:
2702 1646 711
BsaBI GATnn'nnATC
Cuts at: 5053 5053
Size: 5059
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2866 3248 3883 275
Size: 53 83 186 936 1333 382 635 1451
Fragments arranged by size:
1451 1333 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2169 3492 3890 4164 4311 4722 2169
Size: 1323 398 274 147 411 2506
Fragments arranged by size:
2506 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4834
Fragments arranged by size:
4834 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5059
BseYI C'CCAG_C
Cuts at: 4262 4758 4262
Size: 496 4563
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 1163
Size: 573 369 4117
Fragments arranged by size:
4117 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2509 3270 4298 1540
Size: 275 186 508 761 1028 2301
Fragments arranged by size:
2301 1028 761 508 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 3120 3205 4276 2073
Size: 1047 85 1071 2856
Fragments arranged by size:
2856 1071 1047 85
BsmI GAATG_Cn'
Cuts at: 2570 2663 2570
Size: 93 4966
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2271 2378 3120 3205 4276 1391
Size: 399 283 198 107 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 198 107 85
BspEI T'CCGG_A
Cuts at: 4722 4722
Size: 5059
BspHI T'CATG_A
Cuts at: 2948 4678 2948
Size: 1730 3329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 2414 844
Size: 1460 110 3489
Fragments arranged by size:
3489 1460 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2946 1394
Size: 429 1123 3507
Fragments arranged by size:
3507 1123 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3554 3728 65
Size: 1183 2306 174 1396
Fragments arranged by size:
2306 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3703 3890 1700
Size: 110 1893 187 2869
Fragments arranged by size:
2869 1893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5059
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5059
BssSI C'ACGA_G
Cuts at: 3119 4131 3119
Size: 1012 4047
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2022 794
Size: 1228 3831
BstBI TT'CG_AA
Cuts at: 3863 3863
Size: 5059
BstEII G'GTnAC_C
Cuts at: 1184 2245 1184
Size: 1061 3998
BstYI r'GATC_y
Cuts at: 1972 2224 2389 2756 3141 3158 4610 4696 4708
Size: 252 165 367 385 17 1452 86 12
Cuts at: 4708 5054 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 252 165 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2274 513
Size: 552 1209 3298
Fragments arranged by size:
3298 1209 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4320
Fragments arranged by size:
4320 683 56
BtsI GCAGTG_nn'
Cuts at: 2575 3369 3389 2575
Size: 794 20 4245
Fragments arranged by size:
4245 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5059
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5024
DraI TTT'AAA
Cuts at: 2363 2363
Size: 5059
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4066 809
Size: 507 2750 1802
Fragments arranged by size:
2750 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2506 3395 5024
Size: 54 1425 50 275 18 676 889 1629
Cuts at: 5024 8
Size: 43
Fragments arranged by size:
1629 1425 889 676 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2506 1537
Size: 275 694 4090
Fragments arranged by size:
4090 694 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 2328 2989 1353
Size: 782 193 661 3423
Fragments arranged by size:
3423 782 661 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3646 4020 4166 387
Size: 1064 138 2057 374 146 1280
Fragments arranged by size:
2057 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 3122 3719 4506 4774 1239
Size: 393 484 1006 597 787 268 1524
Fragments arranged by size:
1524 1006 787 597 484 393 268
EcoRI G'AATT_C
Cuts at: 2368 2746 2368
Size: 378 4681
FspI TGC'GCA
Cuts at: 2300 3565 2300
Size: 1265 3794
HaeII r_GCGC'y
Cuts at: 1678 4206 1678
Size: 2528 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2173 2205 3700 3732 3774
Size: 32 135 32 601 32 1495 32 42
Cuts at: 3774 3806 1373
Size: 32 2626
Fragments arranged by size:
2626 1495 601 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2403 2649 669
Size: 988 281 465 246 3079
Fragments arranged by size:
3079 988 465 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5059
HpaI GTT'AAC
Cuts at: 2649 2649
Size: 5059
KpnI G_GTAC'C
Cuts at: 2154 2384 2154
Size: 230 4829
MfeI C'AATT_G
Cuts at: 2658 2658
Size: 5059
MluI A'CGCG_T
Cuts at: 4935 4935
Size: 5059
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2171 4173 4357 1305
Size: 866 2002 184 2007
Fragments arranged by size:
2007 2002 866 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3237
Fragments arranged by size:
3237 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3376 3535 518
Size: 648 1851 359 159 2042
Fragments arranged by size:
2042 1851 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2475 3152 4300 4545 1606
Size: 416 453 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 453 416 245
NcoI C'CATG_G
Cuts at: 513 1065 2274 513
Size: 552 1209 3298
Fragments arranged by size:
3298 1209 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5059
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5059
NotI GC'GGCC_GC
Cuts at: 2506 2506
Size: 5059
NspI r_CATG'y
Cuts at: 1132 1229 2417 3962 1132
Size: 97 1188 1545 2229
Fragments arranged by size:
2229 1545 1188 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5059
PciI A'CATG_T
Cuts at: 3958 3958
Size: 5059
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5059
PmeI GTTT'AAAC
Cuts at: 2363 2363
Size: 5059
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 4641 4673 3128
Size: 32 1481 32 3514
Fragments arranged by size:
3514 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5059
PsiI TTA'TAA
Cuts at: 2629 2629
Size: 5059
PstI C_TGCA'G
Cuts at: 830 2027 2411 830
Size: 1197 384 3478
Fragments arranged by size:
3478 1197 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5059
PvuII CAG'CTG
Cuts at: 2022 2022
Size: 5059
SalI G'TCGA_C
Cuts at: 1936 2401 1936
Size: 465 4594
SapI GCTCTTCn'nnn_
Cuts at: 2135 2135
Size: 5059
SbfI CC_TGCA'GG
Cuts at: 2411 2411
Size: 5059
ScaI AGT'ACT
Cuts at: 3307 5019 3307
Size: 1712 3347
SfcI C'TryA_G
Cuts at: 826 1045 2023 2104 2407 3542 4223 4414 826
Size: 219 978 81 303 1135 681 191 1471
Fragments arranged by size:
1471 1135 978 681 303 219 191 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5059
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5059
SmaI CCC'GGG
Cuts at: 1872 2098 2145 2386 1872
Size: 226 47 241 4545
Fragments arranged by size:
4545 241 226 47
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3163 4064 4326 820
Size: 197 926 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2850 493
Size: 2357 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5059
SphI G_CATG'C
Cuts at: 1132 2417 1132
Size: 1285 3774
SspI AAT'ATT
Cuts at: 5 52 2983 5
Size: 47 2931 2081
Fragments arranged by size:
2931 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5059
StyI C'CwwG_G
Cuts at: 513 1065 2219 2274 2498 513
Size: 552 1154 55 224 3074
Fragments arranged by size:
3074 1154 552 224 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3103 3256 3441 3886 325
Size: 1255 1506 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3305 5017 96
Size: 274 80 33 51 1443 1328 1712 138
Fragments arranged by size:
1712 1443 1328 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5019 5051 5019
Size: 32 5027
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5059
XbaI T'CTAG_A
Cuts at: 2395 2395
Size: 5059
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2062
Size: 5059
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5059
XmaI C'CCGG_G
Cuts at: 1870 2096 2143 2384 1870
Size: 226 47 241 4545
Fragments arranged by size:
4545 241 226 47
XmnI GAAnn'nnTTC
Cuts at: 3188 3188
Size: 5059
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2867 276
Size: 53 83 186 936 1333 2468
Fragments arranged by size:
2468 1333 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI