Restriction map for
pFN21AE1515
(Circular) (Six-base) MAPSORT of: pFN21AE1515.seq Check: 65 from: 1 to: 5485
pFN21AE1515 5485 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2577 3295 278
Size: 53 83 186 936 1041 718 2468
Fragments arranged by size:
2468 1041 936 718 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 3548 4932 5200 1632
Size: 402 1514 1384 268 1917
Fragments arranged by size:
1917 1514 1384 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 2113 4214 2113
Size: 2101 3384
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 2483 3228 4800 2483
Size: 745 1572 3168
Fragments arranged by size:
3168 1572 745
ApaI G_GGCC'C
Cuts at: 2773 2781 2773
Size: 8 5477
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2171 2794 3020 3172 5281 1493
Size: 678 623 226 152 2109 1697
Fragments arranged by size:
2109 1697 678 623 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2766 2810 1870
Size: 73 823 44 4545
Fragments arranged by size:
4545 823 73 44
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2723 2806 4261 618
Size: 325 217 627 936 83 1455 1842
Fragments arranged by size:
1842 1455 936 627 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2056 2773 2781 2804 1391
Size: 665 717 8 23 4072
Fragments arranged by size:
4072 717 665 23 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2022 928
Size: 971 123 4391
Fragments arranged by size:
4391 971 123
BbvCI CC'TCA_GC
Cuts at: 2563 2563
Size: 5485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3676 3710 1903
Size: 34 50 34 1655 34 3678
Fragments arranged by size:
3678 1655 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 2709 4096
Size: 107 122 71 631 605 430 607 1387
Cuts at: 4096 136
Size: 1525
Fragments arranged by size:
1525 1387 631 607 605 430 122 107
71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2902 2902
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 1790 2562 2686 2726 2773 2781 3546 4702 1790
Size: 772 124 40 47 8 765 1156 2573
Fragments arranged by size:
2573 1156 772 765 124 47 40 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2608 2638 3216
Size: 416 53 83 399 24 1191 30 578
Cuts at: 3216 4174 442
Size: 958 1753
Fragments arranged by size:
1753 1191 958 578 416 399 83 53
30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 4145 1239
Size: 1013 1893 2579
Fragments arranged by size:
2579 1893 1013
Bpu10I CC'TnA_GC
Cuts at: 2265 2563 2265
Size: 298 5187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 2606 3610 4475 4773 2068
Size: 538 1004 865 298 2780
Fragments arranged by size:
2780 1004 865 538 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 4148 882
Size: 617 711 1938 2219
Fragments arranged by size:
2219 1938 711 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2574 3292 3674 4309
Size: 53 83 186 936 1041 718 382 635
Cuts at: 4309 275
Size: 1451
Fragments arranged by size:
1451 1041 936 718 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 1178
Size: 30 165 30 747 30 4483
Fragments arranged by size:
4483 747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2100 2249 2609 1186
Size: 914 149 360 4062
Fragments arranged by size:
4062 914 360 149
BseYI C'CCAG_C
Cuts at: 2090 2392 2698 4688 5184 2090
Size: 302 306 1990 496 2391
Fragments arranged by size:
2391 1990 496 306 302
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4912
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3696 4724 1540
Size: 275 186 934 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2448 2598 2679 3546 3631 4702 2448
Size: 150 81 867 85 1071 3231
Fragments arranged by size:
3231 1071 867 150 85 81
BsmI GAATG_Cn'
Cuts at: 2996 3089 2996
Size: 93 5392
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2448 2562 2598 2679 2686 2726
Size: 399 266 392 114 36 81 7 40
Cuts at: 2726 2773 2781 2804 3546 3631 4702 1391
Size: 47 8 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 392 266 114 85
81 47 40 36 23 8 7
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2840 844
Size: 1996 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2617 3372 1394
Size: 429 794 755 3507
Fragments arranged by size:
3507 794 755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2283 2473 3980 4154 65
Size: 1183 1035 190 1507 174 1396
Fragments arranged by size:
1507 1396 1183 1035 190 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4129 4316 1700
Size: 110 2319 187 2869
Fragments arranged by size:
2869 2319 187 110
BsrGI T'GTAC_A
Cuts at: 96 2134 96
Size: 2038 3447
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 3545 4557 3545
Size: 1012 4473
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5485
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 2362 1184
Size: 1178 4307
BstYI r'GATC_y
Cuts at: 1972 2200 2815 3182 3567 3584 5036 5122 5134
Size: 228 615 367 385 17 1452 86 12
Cuts at: 5134 5480 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 615 385 367 346 228 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2358 2358
Size: 5485
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4933
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3795 3815 3001
Size: 794 20 4671
Fragments arranged by size:
4671 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 2755 2932
Size: 54 1425 50 275 18 412 513 177
Cuts at: 2932 3821 5450 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 513 412 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2510 3415 1353
Size: 1157 905 3423
Fragments arranged by size:
3423 1157 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 4072 4446 4592 387
Size: 1064 138 531 1952 374 146 1280
Fragments arranged by size:
1952 1280 1064 531 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2252 3548 4145 4932 5200 1239
Size: 393 402 218 1296 597 787 268 1524
Fragments arranged by size:
1524 1296 787 597 402 393 268 218
EcoO109I rG'GnC_Cy
Cuts at: 2777 2778 2777
Size: 1 5484
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 4632 1678
Size: 2954 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 2099 2131 4126
Size: 32 135 32 456 32 39 32 1995
Cuts at: 4126 4158 4200 4232 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1995 456 135 42 39 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2118 2829 3075 669
Size: 988 281 180 711 246 3079
Fragments arranged by size:
3079 988 711 281 246 180
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2352 4599 4783 1305
Size: 1047 2247 184 2007
Fragments arranged by size:
2247 2007 1047 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2757 10
Size: 54 1425 343 925 2738
Fragments arranged by size:
2738 1425 925 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2461 2717 3443 3802 3961 518
Size: 648 1295 256 726 359 159 2042
Fragments arranged by size:
2042 1295 726 648 359 256 159
MspA1I CmG'CkG
Cuts at: 1606 2901 3578 4726 4971 1606
Size: 1295 677 1148 245 2120
Fragments arranged by size:
2120 1295 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4933
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2843 4388 1132
Size: 97 1614 1545 2229
Fragments arranged by size:
2229 1614 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2686 2773 1348
Size: 1338 87 4060
Fragments arranged by size:
4060 1338 87
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PspOMI G'GGCC_C
Cuts at: 2769 2777 2769
Size: 8 5477
PstI C_TGCA'G
Cuts at: 830 2588 2837 830
Size: 1758 249 3478
Fragments arranged by size:
3478 1758 249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2584 2833 3968 4649 4840 826
Size: 219 1539 249 1135 681 191 1471
Fragments arranged by size:
1539 1471 1135 681 249 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2768 2812 1872
Size: 896 44 4545
Fragments arranged by size:
4545 896 44
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2047 2621 3589 4490 4752 820
Size: 197 926 104 574 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 574 262 197 104
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2061 2376 2924 513
Size: 552 996 315 548 3074
Fragments arranged by size:
3074 996 552 548 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3512 3529 3682 3867 4312 325
Size: 1255 1932 17 153 185 445 1498
Fragments arranged by size:
1932 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2134 3731 5443
Size: 274 80 33 51 1443 157 1597 1712
Cuts at: 5443 96
Size: 138
Fragments arranged by size:
1712 1597 1443 274 157 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2383
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2766 2810 1870
Size: 896 44 4545
Fragments arranged by size:
4545 896 44
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2575 3293 276
Size: 53 83 186 936 1041 718 2468
Fragments arranged by size:
2468 1041 936 718 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI