Restriction map for
pFN21AE1510
(Circular) (Six-base) MAPSORT of: pFN21AE1510.seq Check: 4798 from: 1 to: 6850
pFN21AE1510 6850 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3529 3529
Size: 6850
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4660 278
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4193 1937
Size: 2256 4594
Acc65I G'GTAC_C
Cuts at: 4171 4171
Size: 6850
AclI AA'CG_TT
Cuts at: 1563 4977 5350 1563
Size: 3414 373 3063
Fragments arranged by size:
3414 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 2862 2964 3852 3948 4037 4913 6297
Size: 956 274 102 888 96 89 876 1384
Cuts at: 6297 6565 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 956 888 876 274 268 102
96 89
AfeI AGC'GCT
Cuts at: 2110 2110
Size: 6850
AflII C'TTAA_G
Cuts at: 820 1017 3736 820
Size: 197 2719 3934
Fragments arranged by size:
3934 2719 197
AflIII A'CryG_T
Cuts at: 1203 2425 3318 5749 6726 1203
Size: 1222 893 2431 977 1327
Fragments arranged by size:
2431 1327 1222 977 893
AgeI A'CCGG_T
Cuts at: 5681 5681
Size: 6850
AhdI GACnn_n'nnGTC
Cuts at: 5579 5579
Size: 6850
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6850
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 5734
AlwNI CAG_nnn'CTG
Cuts at: 2237 4593 6165 2237
Size: 2356 1572 2922
Fragments arranged by size:
2922 2356 1572
ApaLI G'TGCA_C
Cuts at: 4907 6063 4907
Size: 1156 5694
ApoI r'AATT_y
Cuts at: 1493 2523 2880 2948 2985 3114 3257 3601 3610
Size: 1030 357 68 37 129 143 344 9
Cuts at: 3610 3775 4159 4385 4537 6646 1493
Size: 165 384 226 152 2109 1697
Fragments arranged by size:
2109 1697 1030 384 357 344 226 165
152 143 129 68 37 9
AseI AT'TA_AT
Cuts at: 160 1032 5404 160
Size: 872 4372 1606
Fragments arranged by size:
4372 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 4175 1870
Size: 73 94 2138 4545
Fragments arranged by size:
4545 2138 94 73
BamHI G'GATC_C
Cuts at: 3228 3741 4180 4547 3228
Size: 513 439 367 5531
Fragments arranged by size:
5531 513 439 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2516 3517 4171 5626
Size: 325 217 627 305 424 1001 654 1455
Cuts at: 5626 618
Size: 1842
Fragments arranged by size:
1842 1455 1001 654 627 424 325 305
217
BanII G_rGCy'C
Cuts at: 1391 4169 1391
Size: 2778 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2942 3840 928
Size: 971 1043 898 3938
Fragments arranged by size:
3938 1043 971 898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5041 5075 1903
Size: 34 3104 34 3678
Fragments arranged by size:
3678 3104 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4742 5952 4742
Size: 1210 5640
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6850
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5461 136
Size: 107 122 71 631 605 3789 1525
Fragments arranged by size:
3789 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6845 6845
Size: 6850
BlpI GC'TnA_GC
Cuts at: 4267 4267
Size: 6850
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2103 4911 6067 1790
Size: 307 6 2808 1156 2573
Fragments arranged by size:
2808 2573 1156 307 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6850
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4581 5539 442
Size: 416 53 83 399 24 3164 958 1753
Fragments arranged by size:
3164 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3681 1056
Size: 2625 4225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2105 2382 3064 5510 1239
Size: 850 16 277 682 2446 2579
Fragments arranged by size:
2579 2446 850 682 277 16
Bpu10I CC'TnA_GC
Cuts at: 2468 2468
Size: 6850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4058 4975 5840 6138 4058
Size: 917 865 298 4770
Fragments arranged by size:
4770 917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5513 882
Size: 617 4014 2219
Fragments arranged by size:
4014 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3321 4641 493
Size: 711 2117 1320 2702
Fragments arranged by size:
2702 2117 1320 711
BsaBI GATnn'nnATC
Cuts at: 2907 3054 6844 2907
Size: 147 3790 2913
Fragments arranged by size:
3790 2913 147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4657 5039 5674 275
Size: 53 83 186 936 3124 382 635 1451
Fragments arranged by size:
3124 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3549 5283 5681 5955 6102 6513 3549
Size: 1734 398 274 147 411 3886
Fragments arranged by size:
3886 1734 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6625
Fragments arranged by size:
6625 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3701 3704 3770 3779 1186
Size: 2515 3 66 9 4257
Fragments arranged by size:
4257 2515 66 9 3
BseYI C'CCAG_C
Cuts at: 2472 3162 4131 6053 6549 2472
Size: 690 969 1922 496 2773
Fragments arranged by size:
2773 1922 969 690 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2174 2306 3431 1163
Size: 573 399 39 132 1125 4582
Fragments arranged by size:
4582 1125 573 399 132 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4300 5061 6089 1540
Size: 275 186 2299 761 1028 2301
Fragments arranged by size:
2301 2299 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4911 4996 6067 4911
Size: 85 1071 5694
Fragments arranged by size:
5694 1071 85
BsmI GAATG_Cn'
Cuts at: 2592 2852 4361 4454 2592
Size: 260 1509 93 4988
Fragments arranged by size:
4988 1509 260 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2103 4169 4911 4996 6067 1391
Size: 399 307 6 2066 742 85 1071 2174
Fragments arranged by size:
2174 2066 1071 742 399 307 85 6
BspEI T'CCGG_A
Cuts at: 3549 6513 3549
Size: 2964 3886
BspHI T'CATG_A
Cuts at: 4739 6469 4739
Size: 1730 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 3529 4205 844
Size: 1209 1476 676 3489
Fragments arranged by size:
3489 1476 1209 676
BsrBI CCG'CTC
Cuts at: 1394 1823 3475 4737 1394
Size: 429 1652 1262 3507
Fragments arranged by size:
3507 1652 1262 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2835 2937 5345 5519 65
Size: 1183 1587 102 2408 174 1396
Fragments arranged by size:
2408 1587 1396 1183 174 102
BsrFI r'CCGG_y
Cuts at: 1700 1810 5494 5681 1700
Size: 110 3684 187 2869
Fragments arranged by size:
3684 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6850
BssSI C'ACGA_G
Cuts at: 2545 3342 4910 5922 2545
Size: 797 1568 1012 3473
Fragments arranged by size:
3473 1568 1012 797
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6850
BstBI TT'CG_AA
Cuts at: 5654 5654
Size: 6850
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6850
BstXI CCAn_nnnn'nTGG
Cuts at: 3580 3580
Size: 6850
BstYI r'GATC_y
Cuts at: 1972 3228 3414 3741 4180 4547 4932 4949 6401
Size: 1256 186 327 439 367 385 17 1452
Cuts at: 6401 6487 6499 6845 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1256 439 385 367 346 327
186 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2863 513
Size: 552 939 859 4500
Fragments arranged by size:
4500 939 859 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6111
Fragments arranged by size:
6111 683 56
BtsI GCAGTG_nn'
Cuts at: 4366 5160 5180 4366
Size: 794 20 6036
Fragments arranged by size:
6036 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6815
DraI TTT'AAA
Cuts at: 4154 4154
Size: 6850
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2126 5857 809
Size: 507 810 3731 1802
Fragments arranged by size:
3731 1802 810 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 4297 5186
Size: 54 1425 50 275 18 327 2140 889
Cuts at: 5186 6815 8
Size: 1629 43
Fragments arranged by size:
2140 1629 1425 889 327 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4297 1537
Size: 275 2485 4090
Fragments arranged by size:
4090 2485 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 3885 4051 4780 1353
Size: 669 1863 166 729 3423
Fragments arranged by size:
3423 1863 729 669 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5437 5811 5957 387
Size: 1064 138 3848 374 146 1280
Fragments arranged by size:
3848 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2105 2382 2588 2862 2964 3064
Size: 393 457 16 277 206 274 102 100
Cuts at: 3064 3852 3948 4037 4913 5510 6297 6565 1239
Size: 788 96 89 876 597 787 268 1524
Fragments arranged by size:
1524 876 788 787 597 457 393 277
274 268 206 102 100 96 89 16
EcoRI G'AATT_C
Cuts at: 3257 3610 3775 4159 4537 3257
Size: 353 165 384 378 5570
Fragments arranged by size:
5570 384 378 353 165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2483 2483
Size: 6850
FspI TGC'GCA
Cuts at: 5356 5356
Size: 6850
HaeII r_GCGC'y
Cuts at: 1678 2112 5997 1678
Size: 434 3885 2531
Fragments arranged by size:
3885 2531 434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3230 3262 3297 3329 5491
Size: 32 135 32 1658 32 35 32 2162
Cuts at: 5491 5523 5565 5597 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2162 1658 135 42 35 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3238 4194 4440 669
Size: 988 281 1300 956 246 3079
Fragments arranged by size:
3079 1300 988 956 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6850
HpaI GTT'AAC
Cuts at: 4440 4440
Size: 6850
KpnI G_GTAC'C
Cuts at: 4175 4175
Size: 6850
MfeI C'AATT_G
Cuts at: 4074 4449 4074
Size: 375 6475
MluI A'CGCG_T
Cuts at: 6726 6726
Size: 6850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3363 5964 6148 1305
Size: 2058 2601 184 2007
Fragments arranged by size:
2601 2058 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5028
Fragments arranged by size:
5028 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 4808 5167 5326 518
Size: 648 1623 2019 359 159 2042
Fragments arranged by size:
2042 2019 1623 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2234 2942 4266 4943 6091 6336 1606
Size: 628 708 1324 677 1148 245 2120
Fragments arranged by size:
2120 1324 1148 708 677 628 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5359
Fragments arranged by size:
5359 939 552
NdeI CA'TA_TG
Cuts at: 387 3250 387
Size: 2863 3987
NheI G'CTAG_C
Cuts at: 1052 3677 1052
Size: 2625 4225
NotI GC'GGCC_GC
Cuts at: 4297 4297
Size: 6850
NspI r_CATG'y
Cuts at: 1132 1229 2429 4208 5753 1132
Size: 97 1200 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 1200 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6850
PciI A'CATG_T
Cuts at: 2425 5749 2425
Size: 3324 3526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4146 1108
Size: 3038 3812
PfoI T'CCnGG_A
Cuts at: 3898 3898
Size: 6850
PmeI GTTT'AAAC
Cuts at: 4154 4154
Size: 6850
PmlI CAC'GTG
Cuts at: 3321 3321
Size: 6850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 6432 6464 4919
Size: 32 1481 32 5305
Fragments arranged by size:
5305 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6850
PsiI TTA'TAA
Cuts at: 2666 4420 2666
Size: 1754 5096
PstI C_TGCA'G
Cuts at: 830 2064 4202 830
Size: 1234 2138 3478
Fragments arranged by size:
3478 2138 1234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6850
PvuII CAG'CTG
Cuts at: 2234 2942 2234
Size: 708 6142
SalI G'TCGA_C
Cuts at: 1936 4192 1936
Size: 2256 4594
SbfI CC_TGCA'GG
Cuts at: 4202 4202
Size: 6850
ScaI AGT'ACT
Cuts at: 5098 6810 5098
Size: 1712 5138
SexAI A'CCwGG_T
Cuts at: 2142 2577 2142
Size: 435 6415
SfcI C'TryA_G
Cuts at: 826 1045 2060 2954 4198 5333 6014 6205 826
Size: 219 1015 894 1244 1135 681 191 1471
Fragments arranged by size:
1471 1244 1135 1015 894 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6850
SmaI CCC'GGG
Cuts at: 1872 2039 4177 1872
Size: 167 2138 4545
Fragments arranged by size:
4545 2138 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4037 4954 5855 6117 820
Size: 197 926 1793 301 917 901 262 1553
Fragments arranged by size:
1793 1553 926 917 901 301 262 197
SnaBI TAC'GTA
Cuts at: 493 4641 493
Size: 4148 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6850
SphI G_CATG'C
Cuts at: 1132 4208 1132
Size: 3076 3774
SrfI GCCC'GGGC
Cuts at: 2039 2039
Size: 6850
SspI AAT'ATT
Cuts at: 5 52 4774 5
Size: 47 4722 2081
Fragments arranged by size:
4722 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6850
StyI C'CwwG_G
Cuts at: 513 1065 2004 2512 3394 3427 3781 4289 513
Size: 552 939 508 882 33 354 508 3074
Fragments arranged by size:
3074 939 882 552 508 508 354 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 3378 3926 4120 4877 4894 5047
Size: 1255 681 1117 548 194 757 17 153
Cuts at: 5047 5232 5677 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1117 757 681 548 445 194
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5096 6808 96
Size: 274 80 33 51 1443 3119 1712 138
Fragments arranged by size:
3119 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2446 2478 3393 3425 6810 6842 2446
Size: 32 915 32 3385 32 2454
Fragments arranged by size:
3385 2454 915 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6850
XbaI T'CTAG_A
Cuts at: 4186 4186
Size: 6850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2585 3248 3768 2585
Size: 663 520 5667
Fragments arranged by size:
5667 663 520
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6850
XmaI C'CCGG_G
Cuts at: 1870 2037 4175 1870
Size: 167 2138 4545
Fragments arranged by size:
4545 2138 167
XmnI GAAnn'nnTTC
Cuts at: 2949 3894 4979 2949
Size: 945 1085 4820
Fragments arranged by size:
4820 1085 945
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4658 276
Size: 53 83 186 936 3124 2468
Fragments arranged by size:
3124 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SwaI