Restriction map for
pFN21AE1509
(Circular) (Six-base) MAPSORT of: pFN21AE1509.seq Check: 1334 from: 1 to: 5803
pFN21AE1509 5803 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3866 5250 5518 1632
Size: 2234 1384 268 1917
Fragments arranged by size:
2234 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 2638 2847 4702 5679 1203
Size: 1435 209 1855 977 1327
Fragments arranged by size:
1855 1435 1327 977 209
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 4532 4532
Size: 5803
AleI CACnn'nnGTG
Cuts at: 1166 2108 2567 1166
Size: 942 459 4402
Fragments arranged by size:
4402 942 459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5803
AlwNI CAG_nnn'CTG
Cuts at: 3546 5118 3546
Size: 1572 4231
ApaI G_GGCC'C
Cuts at: 2391 2692 2391
Size: 301 5502
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 3112 3338 3490 5599 1493
Size: 1619 226 152 2109 1697
Fragments arranged by size:
2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 3128 1870
Size: 73 101 1084 4545
Fragments arranged by size:
4545 1084 101 73
AvrII C'CTAG_G
Cuts at: 2974 2974
Size: 5803
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2221 2397 2821 3124
Size: 325 217 627 230 204 176 424 303
Cuts at: 3124 4579 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 424 325 303 230 217
204 176
BanII G_rGCy'C
Cuts at: 1391 2053 2136 2391 2692 2883 3017 3074 3122
Size: 662 83 255 301 191 134 57 48
Cuts at: 3122 1391
Size: 4072
Fragments arranged by size:
4072 662 301 255 191 134 83 57
48
BbeI G_GCGC'C
Cuts at: 2021 2225 2401 2021
Size: 204 176 5423
Fragments arranged by size:
5423 204 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4832
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3994 4028 1903
Size: 34 50 34 1973 34 3678
Fragments arranged by size:
3678 1973 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2395 2531 4414
Size: 107 122 71 631 605 723 136 1883
Cuts at: 4414 136
Size: 1525
Fragments arranged by size:
1883 1525 723 631 605 136 122 107
71
BglII A'GATC_T
Cuts at: 5798 5798
Size: 5803
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2564 2692 3067 3864 5020 1790
Size: 601 173 128 375 797 1156 2573
Fragments arranged by size:
2573 1156 797 601 375 173 128
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5803
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3534 4492 442
Size: 416 53 83 399 24 2117 958 1753
Fragments arranged by size:
2117 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3057 3057
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2576 2612 2850 4463 1239
Size: 1106 231 36 238 1613 2579
Fragments arranged by size:
2579 1613 1106 238 231 36
Bpu10I CC'TnA_GC
Cuts at: 3016 3016
Size: 5803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 2355 2765 4466 882
Size: 617 561 295 410 1701 2219
Fragments arranged by size:
2219 1701 617 561 410 295
BsaAI yAC'GTr
Cuts at: 493 1204 2158 2565 2641 2850 3594 493
Size: 711 954 407 76 209 744 2702
Fragments arranged by size:
2702 954 744 711 407 209 76
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2193 2222 2398
Size: 53 83 186 936 485 175 29 176
Cuts at: 2398 3610 3992 4627 275
Size: 1212 382 635 1451
Fragments arranged by size:
1451 1212 936 635 485 382 186 176
175 83 53 29
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 2549 2579 2970
Size: 30 165 30 1050 30 66 30 391
Cuts at: 2970 3000 1178
Size: 30 3981
Fragments arranged by size:
3981 1050 391 165 66 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 1186
Size: 1207 4596
BseYI C'CCAG_C
Cuts at: 2505 2685 2910 5006 5502 2505
Size: 180 225 2096 496 2806
Fragments arranged by size:
2806 2096 496 225 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2879 1163
Size: 573 1143 4087
Fragments arranged by size:
4087 1143 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 3253 4014 5042 1540
Size: 275 186 124 1128 761 1028 2301
Fragments arranged by size:
2301 1128 1028 761 275 186 124
BsiHKAI G_wGCw'C
Cuts at: 3017 3864 3949 5020 3017
Size: 847 85 1071 3800
Fragments arranged by size:
3800 1071 847 85
BsmI GAATG_Cn'
Cuts at: 3314 3407 3314
Size: 93 5710
BsmBI CGTCTCn'nnnn_
Cuts at: 2600 2662 2600
Size: 62 5741
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2136 2391 2564 2692 2883 3017
Size: 399 263 83 255 173 128 191 134
Cuts at: 3017 3067 3074 3122 3864 3949 5020 1391
Size: 50 7 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 263 255 191 173
134 128 85 83 50 48 7
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 2472 3158 844
Size: 1481 147 686 3489
Fragments arranged by size:
3489 1481 686 147
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4447 4634 1700
Size: 110 2637 187 2869
Fragments arranged by size:
2869 2637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 2617 1925
Size: 692 5111
BssSI C'ACGA_G
Cuts at: 2864 3863 4875 2864
Size: 999 1012 3792
Fragments arranged by size:
3792 1012 999
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 794
Size: 1382 4421
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstYI r'GATC_y
Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798
Size: 1161 367 385 17 1452 86 12 346
Cuts at: 5798 1972
Size: 1977
Fragments arranged by size:
1977 1452 1161 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2678 2678
Size: 5803
BtgI C'CryG_G
Cuts at: 513 1065 2125 2216 2225 2407 2481 513
Size: 552 1060 91 9 182 74 3835
Fragments arranged by size:
3835 1060 552 182 91 74 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 2431 3073 487
Size: 683 56 902 303 642 3217
Fragments arranged by size:
3217 902 683 642 303 56
BtsI GCAGTG_nn'
Cuts at: 2154 3319 4113 4133 2154
Size: 1165 794 20 3824
Fragments arranged by size:
3824 1165 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 3250 4139
Size: 54 1425 50 275 18 292 1128 889
Cuts at: 4139 5768 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1128 889 292 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2122 3250 1537
Size: 275 310 1128 4090
Fragments arranged by size:
4090 1128 310 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2829 3005 3733 1353
Size: 1476 176 728 3423
Fragments arranged by size:
3423 1476 728 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2413 2549 4390 4764 4910 387
Size: 1064 138 824 136 1841 374 146 1280
Fragments arranged by size:
1841 1280 1064 824 374 146 138 136
EcoICRI GAG'CTC
Cuts at: 3015 3015
Size: 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2576 2612 2850 3866 4463 5250
Size: 393 713 231 36 238 1016 597 787
Cuts at: 5250 5518 1239
Size: 268 1524
Fragments arranged by size:
1524 1016 787 713 597 393 268 238
231 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2456 2456
Size: 5803
EcoO109I rG'GnC_Cy
Cuts at: 2387 2461 2689 2387
Size: 74 228 5501
Fragments arranged by size:
5501 228 74
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FspI TGC'GCA
Cuts at: 4309 4309
Size: 5803
HaeII r_GCGC'y
Cuts at: 1678 2021 2225 2284 2401 4950 1678
Size: 343 204 59 117 2549 2531
Fragments arranged by size:
2549 2531 343 204 117 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2333 2365 2453 2485 2635
Size: 32 135 32 761 32 88 32 150
Cuts at: 2635 2667 4444 4476 4518 4550 1373
Size: 32 1777 32 42 32 2626
Fragments arranged by size:
2626 1777 761 150 135 88 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3393 669
Size: 988 281 1209 246 3079
Fragments arranged by size:
3079 1209 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KasI G'GCGC_C
Cuts at: 2017 2221 2397 2017
Size: 204 176 5423
Fragments arranged by size:
5423 204 176
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4917 5101 1305
Size: 3612 184 2007
Fragments arranged by size:
3612 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3981
Fragments arranged by size:
3981 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2147 2567 3761 4120 4279 518
Size: 648 942 39 420 1194 359 159 2042
Fragments arranged by size:
2042 1194 942 648 420 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 2127 2218 2409 2483 2731 2813 3219 3896
Size: 521 91 191 74 248 82 406 677
Cuts at: 3896 5044 5289 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 521 406 248 245 191
91 82 74
NarI GG'CG_CC
Cuts at: 2018 2222 2398 2018
Size: 204 176 5423
Fragments arranged by size:
5423 204 176
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 3161 4706 1132
Size: 97 1932 1545 2229
Fragments arranged by size:
2229 1932 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2692 3067 1348
Size: 1344 375 4084
Fragments arranged by size:
4084 1344 375
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5803
PfoI T'CCnGG_A
Cuts at: 2471 2471
Size: 5803
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PmlI CAC'GTG
Cuts at: 2565 2641 2850 2565
Size: 76 209 5518
Fragments arranged by size:
5518 209 76
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2461 2461
Size: 5803
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2387 2688 2387
Size: 301 5502
PstI C_TGCA'G
Cuts at: 830 2616 3155 830
Size: 1786 539 3478
Fragments arranged by size:
3478 1786 539
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
PvuII CAG'CTG
Cuts at: 2813 2813
Size: 5803
SacI G_AGCT'C
Cuts at: 3017 3017
Size: 5803
SacII CC_GC'GG
Cuts at: 2128 2219 2410 2484 2128
Size: 91 191 74 5447
Fragments arranged by size:
5447 191 91 74
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SapI GCTCTTCn'nnn_
Cuts at: 2829 3005 2829
Size: 176 5627
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 2612 3151 4286 4967 5158 826
Size: 219 1567 539 1135 681 191 1471
Fragments arranged by size:
1567 1471 1135 681 539 219 191
SfoI GGC'GCC
Cuts at: 2019 2223 2399 2019
Size: 204 176 5423
Fragments arranged by size:
5423 204 176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3130 1872
Size: 1258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 2974 3242 513
Size: 552 1909 268 3074
Fragments arranged by size:
3074 1909 552 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2218 2313 2519 2643 3830 3847
Size: 1255 498 140 95 206 124 1187 17
Cuts at: 3847 4000 4185 4630 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1187 498 445 206 185 153
140 124 95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 4049 5761
Size: 274 80 33 51 1443 297 1775 1712
Cuts at: 5761 96
Size: 138
Fragments arranged by size:
1775 1712 1443 297 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 2970 3002 5763 5795 2549
Size: 32 389 32 2761 32 2557
Fragments arranged by size:
2761 2557 389 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2369 2926 2011
Size: 358 557 4888
Fragments arranged by size:
4888 557 358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3128 1870
Size: 1258 4545
XmnI GAAnn'nnTTC
Cuts at: 3932 3932
Size: 5803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI
SexAI SfiI SrfI SwaI