Restriction map for
pFN21AE1506
(Circular) (Six-base) MAPSORT of: pFN21AE1506.seq Check: 5990 from: 1 to: 5788
pFN21AE1506 5788 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2272 2272
Size: 5788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 3131 1937
Size: 1078 116 4594
Fragments arranged by size:
4594 1078 116
Acc65I G'GTAC_C
Cuts at: 2969 3109 2969
Size: 140 5648
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3004 3083 3851 5235 5503 1632
Size: 1372 79 768 1384 268 1917
Fragments arranged by size:
1917 1384 1372 768 268 79
AflII C'TTAA_G
Cuts at: 820 1017 2221 820
Size: 197 1204 4387
Fragments arranged by size:
4387 1204 197
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 2246 2986 1166
Size: 1080 740 3968
Fragments arranged by size:
3968 1080 740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 3531 5103 3531
Size: 1572 4216
ApaI G_GGCC'C
Cuts at: 2112 2112
Size: 5788
ApaLI G'TGCA_C
Cuts at: 2069 3845 5001 2069
Size: 1776 1156 2856
Fragments arranged by size:
2856 1776 1156
ApoI r'AATT_y
Cuts at: 1493 2174 2519 2568 3097 3323 3475 5584 1493
Size: 681 345 49 529 226 152 2109 1697
Fragments arranged by size:
2109 1697 681 529 345 226 152 49
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 3044 3113 1870
Size: 73 138 963 69 4545
Fragments arranged by size:
4545 963 138 73 69
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2969 3109 4564 618
Size: 325 217 627 1182 140 1455 1842
Fragments arranged by size:
1842 1455 1182 627 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2112 2374 3107 1391
Size: 721 262 733 4072
Fragments arranged by size:
4072 733 721 262
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2112 3849 5005 1790
Size: 283 39 1737 1156 2573
Fragments arranged by size:
2573 1737 1156 283 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2841 4448 1239
Size: 1602 1607 2579
Fragments arranged by size:
2579 1607 1602
Bpu10I CC'TnA_GC
Cuts at: 2559 2754 2559
Size: 195 5593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 3913 4778 5076 2894
Size: 1019 865 298 3606
Fragments arranged by size:
3606 1019 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 4451 882
Size: 617 585 2367 2219
Fragments arranged by size:
2367 2219 617 585
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2655 3027 1186
Size: 1291 178 372 3947
Fragments arranged by size:
3947 1291 372 178
BseYI C'CCAG_C
Cuts at: 2577 4991 5487 2577
Size: 2414 496 2878
Fragments arranged by size:
2878 2414 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2964 1163
Size: 573 1228 3987
Fragments arranged by size:
3987 1228 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 3849 3934 5005 2073
Size: 1776 85 1071 2856
Fragments arranged by size:
2856 1776 1071 85
BsmI GAATG_Cn'
Cuts at: 2645 3299 3392 2645
Size: 654 93 5041
Fragments arranged by size:
5041 654 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2112 2374 3107 3849 3934 5005
Size: 399 283 39 262 733 742 85 1071
Cuts at: 5005 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 733 399 283 262 85
39
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 2961 3677 5407 2961
Size: 716 1730 3342
Fragments arranged by size:
3342 1730 716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2272 2798 3143 844
Size: 1428 526 345 3489
Fragments arranged by size:
3489 1428 526 345
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 4283 4457 65
Size: 1183 1284 1751 174 1396
Fragments arranged by size:
1751 1396 1284 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 2736 96
Size: 2640 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2068 794
Size: 1274 4514
BstBI TT'CG_AA
Cuts at: 2566 4592 2566
Size: 2026 3762
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 3118 3485 3870 3887 5339 5425 5437 5783
Size: 1146 367 385 17 1452 86 12 346
Cuts at: 5783 1972
Size: 1977
Fragments arranged by size:
1977 1452 1146 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2112 2154 513
Size: 552 951 96 42 4147
Fragments arranged by size:
4147 951 552 96 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 2770 2899 3304 4098 4118 2241
Size: 529 129 405 794 20 3911
Fragments arranged by size:
3911 794 529 405 129 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 2434 2926 3092 2434
Size: 492 166 5130
Fragments arranged by size:
5130 492 166
DraIII CAC_nnn'GTG
Cuts at: 2204 2204
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 4124 5753
Size: 54 1425 50 275 18 1405 889 1629
Cuts at: 5753 8
Size: 43
Fragments arranged by size:
1629 1425 1405 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 3718 1353
Size: 801 1564 3423
Fragments arranged by size:
3423 1564 801
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2841 3004 3083 3851 4448 5235 5503
Size: 393 1209 163 79 768 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 1209 787 768 597 393 268 163
79
EcoO109I rG'GnC_Cy
Cuts at: 2086 2108 2086
Size: 22 5766
EcoRI G'AATT_C
Cuts at: 2519 2568 3097 3475 2519
Size: 49 529 378 4832
Fragments arranged by size:
4832 529 378 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 3034 2434
Size: 600 5188
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 4935 1678
Size: 3257 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373
Size: 32 135 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3132 3378 669
Size: 988 281 1143 51 246 3079
Fragments arranged by size:
3079 1143 988 281 246 51
HindIII A'AGCT_T
Cuts at: 748 3071 748
Size: 2323 3465
HpaI GTT'AAC
Cuts at: 3081 3378 3081
Size: 297 5491
KpnI G_GTAC'C
Cuts at: 2973 3113 2973
Size: 140 5648
MfeI C'AATT_G
Cuts at: 2835 3387 2835
Size: 552 5236
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2399 4902 5086 1305
Size: 1094 2503 184 2007
Fragments arranged by size:
2503 2007 1094 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3966
Fragments arranged by size:
3966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2696 2986 3746 4105 4264 518
Size: 648 1080 450 290 760 359 159 2042
Fragments arranged by size:
2042 1080 760 648 450 359 290 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2788 3204 3881 5029 5274 1606
Size: 459 723 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 723 677 459 416 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5236
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2105 1348
Size: 757 5031
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 2086
Size: 5788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2108 2108
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2064 3140 830
Size: 1234 1076 3478
Fragments arranged by size:
3478 1234 1076
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2065 2788 2065
Size: 723 5065
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2060 2984 3136 4271 4952 5143 826
Size: 219 1015 924 152 1135 681 191 1471
Fragments arranged by size:
1471 1135 1015 924 681 219 191 152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2221 2909 3892 4793 5055 820
Size: 197 926 278 688 983 901 262 1553
Fragments arranged by size:
1553 983 926 901 688 278 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2637 1517
Size: 1120 4668
StyI C'CwwG_G
Cuts at: 513 1065 2383 3227 513
Size: 552 1318 844 3074
Fragments arranged by size:
3074 1318 844 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 2298 2736
Size: 274 80 33 51 1443 186 135 438
Cuts at: 2736 4034 5746 96
Size: 1298 1712 138
Fragments arranged by size:
1712 1443 1298 438 274 186 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 2042 3917 2042
Size: 1875 3913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI