Restriction map for
pFN21AE1503
(Circular) (Six-base) MAPSORT of: pFN21AE1503.seq Check: 5525 from: 1 to: 5476
pFN21AE1503 5476 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2740 3286 278
Size: 53 83 186 936 1204 546 2468
Fragments arranged by size:
2468 1204 936 546 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 2295 2775 3603 3976 1563
Size: 732 480 828 373 3063
Fragments arranged by size:
3063 828 732 480 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 2736 4205 2736
Size: 1469 4007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 3219 4791 3219
Size: 1572 3904
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2785 3011 3163 5272 1493
Size: 1292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 226 152
AscI GG'CGCG_CC
Cuts at: 2169 2169
Size: 5476
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 2385 2801 1870
Size: 73 215 227 416 4545
Fragments arranged by size:
4545 416 227 215 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2325 2358 2325
Size: 33 5443
BamHI G'GATC_C
Cuts at: 2197 2806 3173 2197
Size: 609 367 4500
Fragments arranged by size:
4500 609 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2318 2797 4252 618
Size: 325 217 627 249 282 479 1455 1842
Fragments arranged by size:
1842 1455 627 479 325 282 249 217
BanII G_rGCy'C
Cuts at: 1391 2795 1391
Size: 1404 4072
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 5476
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2182 2444 928
Size: 971 283 262 3960
Fragments arranged by size:
3960 971 283 262
BbvCI CC'TCA_GC
Cuts at: 2482 2482
Size: 5476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2109 2893 2109
Size: 784 4692
Bme1580I G_kGCm'C
Cuts at: 1790 2321 3537 4693 1790
Size: 531 1216 1156 2573
Fragments arranged by size:
2573 1216 1156 531
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2385 2535 4136 1239
Size: 1133 13 150 1601 2579
Fragments arranged by size:
2579 1601 1133 150 13
Bpu10I CC'TnA_GC
Cuts at: 2482 2482
Size: 5476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2361 2423 2717 4139 882
Size: 617 862 62 294 1422 2219
Fragments arranged by size:
2219 1422 862 617 294 62
BsaAI yAC'GTr
Cuts at: 493 1204 2062 3267 493
Size: 711 858 1205 2702
Fragments arranged by size:
2702 1205 858 711
BsaBI GATnn'nnATC
Cuts at: 2202 5470 2202
Size: 3268 2208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2737 3283 3665
Size: 53 83 186 936 504 700 546 382
Cuts at: 3665 4300 275
Size: 635 1451
Fragments arranged by size:
1451 936 700 635 546 504 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3909 4307 4581 4728 5139 3909
Size: 398 274 147 411 4246
Fragments arranged by size:
4246 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5251
Fragments arranged by size:
5251 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2470 1186
Size: 1183 101 4192
Fragments arranged by size:
4192 1183 101
BseYI C'CCAG_C
Cuts at: 2286 4679 5175 2286
Size: 2393 496 2587
Fragments arranged by size:
2587 2393 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 1163
Size: 573 1044 3859
Fragments arranged by size:
3859 1044 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2310 3537 3622 4693 2310
Size: 1227 85 1071 3093
Fragments arranged by size:
3093 1227 1071 85
BsmI GAATG_Cn'
Cuts at: 2247 2987 3080 2247
Size: 740 93 4643
Fragments arranged by size:
4643 740 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2744 2744
Size: 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2321 2795 3537 3622 4693 1391
Size: 399 520 11 474 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 474 399 85 11
BspEI T'CCGG_A
Cuts at: 5139 5139
Size: 5476
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2831 844
Size: 1987 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 3363 1394
Size: 429 290 1250 3507
Fragments arranged by size:
3507 1250 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 2232 2308 2715 3971 4145 65
Size: 1183 984 76 407 1256 174 1396
Fragments arranged by size:
1396 1256 1183 984 407 174 76
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 2055 2084 2092 2169 1925
Size: 130 29 8 77 5232
Fragments arranged by size:
5232 130 77 29 8
BssSI C'ACGA_G
Cuts at: 2595 3536 4548 2595
Size: 941 1012 3523
Fragments arranged by size:
3523 1012 941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2197 2806 3173 3558 3575 5027 5113 5125
Size: 225 609 367 385 17 1452 86 12
Cuts at: 5125 5471 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 609 385 367 346 225 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2328 2328
Size: 5476
BtgI C'CryG_G
Cuts at: 513 1065 2004 2020 2097 2164 2371 513
Size: 552 939 16 77 67 207 3618
Fragments arranged by size:
3618 939 552 207 77 67 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2521 2551 2591
Size: 54 1425 50 275 18 691 30 40
Cuts at: 2591 2610 2698 2923 3812 5441 8
Size: 19 88 225 889 1629 43
Fragments arranged by size:
1629 1425 889 691 275 225 88 54
50 43 40 30 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3406 1353
Size: 2053 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2385 2535 3539 4136 4923 5191
Size: 393 740 13 150 1004 597 787 268
Cuts at: 5191 1239
Size: 1524
Fragments arranged by size:
1524 1004 787 740 597 393 268 150
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2180 2637 2180
Size: 457 5019
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 5476
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2040 2082 4623 1678
Size: 362 42 2541 2531
Fragments arranged by size:
2541 2531 362 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2722 2754 4117 4149 4191
Size: 32 135 32 1150 32 1363 32 42
Cuts at: 4191 4223 1373
Size: 32 2626
Fragments arranged by size:
2626 1363 1150 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2593 2612 10
Size: 54 1425 343 761 19 2874
Fragments arranged by size:
2874 1425 761 343 54 19
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2166 2892 3569 4717 4962 1606
Size: 560 726 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 677 560 245
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 5476
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3985
Fragments arranged by size:
3985 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2834 4379 1132
Size: 97 1605 1545 2229
Fragments arranged by size:
2229 1605 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5476
PfoI T'CCnGG_A
Cuts at: 2193 2193
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PmlI CAC'GTG
Cuts at: 2062 2062
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PstI C_TGCA'G
Cuts at: 830 2828 830
Size: 1998 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
SacII CC_GC'GG
Cuts at: 2167 2167
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2824 3959 4640 4831 826
Size: 219 1779 1135 681 191 1471
Fragments arranged by size:
1779 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 5476
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2160 2387 2803 1872
Size: 288 227 416 4545
Fragments arranged by size:
4545 416 288 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3580 4481 4743 820
Size: 197 926 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SrfI GCCC'GGGC
Cuts at: 2387 2387
Size: 5476
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 2915 513
Size: 552 939 177 734 3074
Fragments arranged by size:
3074 939 734 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5436 5468 5436
Size: 32 5444
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2294 2494 2620 2294
Size: 200 126 5150
Fragments arranged by size:
5150 200 126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2158 2385 2801 1870
Size: 288 227 416 4545
Fragments arranged by size:
4545 416 288 227
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2738 3284 276
Size: 53 83 186 936 1204 546 2468
Fragments arranged by size:
2468 1204 936 546 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SwaI