Restriction map for
pFN21AE1499
(Circular) (Six-base) MAPSORT of: pFN21AE1499.seq Check: 3750 from: 1 to: 13996
pFN21AE1499 13996 bp
With 182 enzymes: SgfI *
March 28, 2012 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3724 4159 4746 4891 3724
Size: 435 587 145 12829
Fragments arranged by size:
12829 587 435 145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4539 5253 6938 11806
Size: 53 83 186 936 3003 714 1685 4868
Cuts at: 11806 278
Size: 2468
Fragments arranged by size:
4868 3003 2468 1685 936 714 186 83
53
AccI GT'mk_AC
Cuts at: 1937 3417 4620 5518 9720 11339 1937
Size: 1480 1203 898 4202 1619 4594
Fragments arranged by size:
4594 4202 1619 1480 1203 898
Acc65I G'GTAC_C
Cuts at: 10716 11317 10716
Size: 601 13395
AclI AA'CG_TT
Cuts at: 1563 2270 12123 12496 1563
Size: 707 9853 373 3063
Fragments arranged by size:
9853 3063 707 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2699 2813 2837 3026 3128 5237 5736
Size: 818 249 114 24 189 102 2109 499
Cuts at: 5736 6681 6815 6827 8357 10256 10689 12059 13443
Size: 945 134 12 1530 1899 433 1370 1384
Cuts at: 13443 13711 1632
Size: 268 1917
Fragments arranged by size:
2109 1917 1899 1530 1384 1370 945 818
499 433 268 249 189 134 114 102
24 12
AfeI AGC'GCT
Cuts at: 4139 4139
Size: 13996
AflII C'TTAA_G
Cuts at: 820 1017 6716 820
Size: 197 5699 8100
Fragments arranged by size:
8100 5699 197
AflIII A'CryG_T
Cuts at: 1203 3183 3339 4017 4095 6643 8172 9765 10088
Size: 1980 156 678 78 2548 1529 1593 323
Cuts at: 10088 10275 11190 12895 13872 1203
Size: 187 915 1705 977 1327
Fragments arranged by size:
2548 1980 1705 1593 1529 1327 977 915
678 323 187 156 78
AgeI A'CCGG_T
Cuts at: 12827 12827
Size: 13996
AhdI GACnn_n'nnGTC
Cuts at: 4411 9100 12725 4411
Size: 4689 3625 5682
Fragments arranged by size:
5682 4689 3625
AleI CACnn'nnGTG
Cuts at: 1166 4040 9695 9846 1166
Size: 2874 5655 151 5316
Fragments arranged by size:
5655 5316 2874 151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5658 7337 9455 11300 1184
Size: 4474 1679 2118 1845 3880
Fragments arranged by size:
4474 3880 2118 1845 1679
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9720 9752 10809 10841 10895 10927 9720
Size: 32 1057 32 54 32 12789
Fragments arranged by size:
12789 1057 54 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2848 2977 3584 3700 4294 4645 4762 8982 10861
Size: 129 607 116 594 351 117 4220 1879
Cuts at: 10861 10886 11152 11739 13311 2848
Size: 25 266 587 1572 3533
Fragments arranged by size:
4220 3533 1879 1572 607 594 587 351
266 129 117 116 25
ApaI G_GGCC'C
Cuts at: 5150 7285 7301 8944 9126 10166 5150
Size: 2135 16 1643 182 1040 8980
Fragments arranged by size:
8980 2135 1643 1040 182 16
ApaLI G'TGCA_C
Cuts at: 3605 12053 13209 3605
Size: 8448 1156 4392
Fragments arranged by size:
8448 4392 1156
ApoI r'AATT_y
Cuts at: 1493 2531 2537 6050 6665 8708 8808 9741 10061
Size: 1038 6 3513 615 2043 100 933 320
Cuts at: 10061 10874 11305 11531 11683 13792 1493
Size: 813 431 226 152 2109 1697
Fragments arranged by size:
3513 2109 2043 1697 1038 933 813 615
431 320 226 152 100 6
AseI AT'TA_AT
Cuts at: 160 1032 12550 160
Size: 872 11518 1606
Fragments arranged by size:
11518 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13996
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 4409 4632 5084 7552 9076 9119
Size: 73 137 2329 223 452 2468 1524 43
Cuts at: 9119 9152 9280 9351 9511 10898 11321 1870
Size: 33 128 71 160 1387 423 4545
Fragments arranged by size:
4545 2468 2329 1524 1387 452 423 223
160 137 128 73 71 43 33
AvrII C'CTAG_G
Cuts at: 10333 10333
Size: 13996
BamHI G'GATC_C
Cuts at: 4770 5463 8138 8790 9024 11326 11693 4770
Size: 693 2675 652 234 2302 367 7073
Fragments arranged by size:
7073 2675 2302 693 652 367 234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 8990 9266 9329 10716
Size: 325 217 627 309 6894 276 63 1387
Cuts at: 10716 11317 12772 618
Size: 601 1455 1842
Fragments arranged by size:
6894 1842 1455 1387 627 601 325 309
276 217 63
BanII G_rGCy'C
Cuts at: 1391 2764 4326 4356 5150 5513 5538 5904 6805
Size: 1373 1562 30 794 363 25 366 901
Cuts at: 6805 6816 7285 7292 7301 7731 8944 9126 9257
Size: 11 469 7 9 430 1213 182 131
Cuts at: 9257 9293 9420 9455 9467 10166 11315 1391
Size: 36 127 35 12 699 1149 4072
Fragments arranged by size:
4072 1562 1373 1213 1149 901 794 699
469 430 366 363 182 131 127 36
35 30 25 12 11 9 7
BbeI G_GCGC'C
Cuts at: 8994 9270 8994
Size: 276 13720
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2673 3969 5124 5245 7918 928
Size: 971 774 1296 1155 121 2673 7006
Fragments arranged by size:
7006 2673 1296 1155 971 774 121
BbvCI CC'TCA_GC
Cuts at: 3029 6413 9810 3029
Size: 3384 3397 7215
Fragments arranged by size:
7215 3397 3384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4082 4116 5065 5099 7448 7482 12187
Size: 34 2145 34 949 34 2349 34 4705
Cuts at: 12187 12221 1903
Size: 34 3678
Fragments arranged by size:
4705 3678 2349 2145 949 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2853 5816 5944 8368 9562 10918 11888 13098 2853
Size: 2963 128 2424 1194 1356 970 1210 3751
Fragments arranged by size:
3751 2963 2424 1356 1210 1194 970 128
BclI T'GATC_A
Cuts at: 1548 2475 4185 5181 8295 10444 1548
Size: 927 1710 996 3114 2149 5100
Fragments arranged by size:
5100 3114 2149 1710 996 927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 2072 5450
Size: 107 122 71 631 605 391 9 3378
Cuts at: 5450 5645 8967 9155 9178 9495 12607 136
Size: 195 3322 188 23 317 3112 1525
Fragments arranged by size:
3378 3322 3112 1525 631 605 391 317
195 188 122 107 71 23 9
BglII A'GATC_T
Cuts at: 4462 4555 8287 13991 4462
Size: 93 3732 5704 4467
Fragments arranged by size:
5704 4467 3732 93
BlpI GC'TnA_GC
Cuts at: 5898 6377 7355 10404 11413 5898
Size: 479 978 3049 1009 8481
Fragments arranged by size:
8481 3049 1009 978 479
Bme1580I G_kGCm'C
Cuts at: 1790 3609 5150 7285 7301 7380 7608 8944 9126
Size: 1819 1541 2135 16 79 228 1336 182
Cuts at: 9126 9332 9803 10166 12057 13213 1790
Size: 206 471 363 1891 1156 2573
Fragments arranged by size:
2573 2135 1891 1819 1541 1336 1156 471
363 228 206 182 79 16
BmgBI CAC'GTC
Cuts at: 1318 4059 4096 6327 8737 11193 1318
Size: 2741 37 2231 2410 2456 4121
Fragments arranged by size:
4121 2741 2456 2410 2231 37
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3924 8940 10871
Size: 416 53 83 399 24 2507 5016 1931
Cuts at: 10871 11727 12685 442
Size: 856 958 1753
Fragments arranged by size:
5016 2507 1931 1753 958 856 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 9324 1056
Size: 8268 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2726 3302 3524 3677 4085 4219 4370 4472
Size: 1487 576 222 153 408 134 151 102
Cuts at: 4472 4670 4733 4826 5216 5497 5765 6386 7082
Size: 198 63 93 390 281 268 621 696
Cuts at: 7082 7150 7207 7247 7631 7793 8699 9269 9373
Size: 68 57 40 384 162 906 570 104
Cuts at: 9373 9481 9515 10529 11123 12656 1239
Size: 108 34 1014 594 1533 2579
Fragments arranged by size:
2579 1533 1487 1014 906 696 621 594
576 570 408 390 384 281 268 222
198 162 153 151 134 108 104 102
93 68 63 57 40 34
Bpu10I CC'TnA_GC
Cuts at: 2899 3029 4217 6413 7520 9810 2899
Size: 130 1188 2196 1107 2290 7085
Fragments arranged by size:
7085 2290 2196 1188 1107 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4991 5009 8333 9866 12121 12986 13284 4991
Size: 18 3324 1533 2255 865 298 5703
Fragments arranged by size:
5703 3324 2255 1533 865 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2560 5925 6648 8738 9067 10097 10577
Size: 617 1061 3365 723 2090 329 1030 480
Cuts at: 10577 10994 12659 882
Size: 417 1665 2219
Fragments arranged by size:
3365 2219 2090 1665 1061 1030 723 617
480 417 329
BsaAI yAC'GTr
Cuts at: 493 1204 5170 5425 5955 7381 9588 10089 10278
Size: 711 3966 255 530 1426 2207 501 189
Cuts at: 10278 10929 10954 11787 493
Size: 651 25 833 2702
Fragments arranged by size:
3966 2702 2207 1426 833 711 651 530
501 255 189 25
BsaBI GATnn'nnATC
Cuts at: 2648 6920 13990 2648
Size: 4272 7070 2654
Fragments arranged by size:
7070 4272 2654
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4386 4536 4982 5174
Size: 53 83 186 936 2853 150 446 192
Cuts at: 5174 5250 6935 7233 7437 7599 8991 9198 9267
Size: 76 1685 298 204 162 1392 207 69
Cuts at: 9267 9297 11181 11803 12185 12820 275
Size: 30 1884 622 382 635 1451
Fragments arranged by size:
2853 1884 1685 1451 1392 936 635 622
446 382 298 207 204 192 186 162
150 83 76 69 53 30
BsaWI w'CCGG_w
Cuts at: 3966 10713 12429 12827 13101 13248 13659 3966
Size: 6747 1716 398 274 147 411 4303
Fragments arranged by size:
6747 4303 1716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3275 3305 3329 3359 4244
Size: 30 165 30 1872 30 24 30 885
Cuts at: 4244 4274 4589 4619 7199 7229 9509 9539 9720
Size: 30 315 30 2580 30 2280 30 181
Cuts at: 9720 9750 10190 10220 10367 10397 10809 10839 10895
Size: 30 440 30 147 30 412 30 56
Cuts at: 10895 10925 11069 11099 1178
Size: 30 144 30 4075
Fragments arranged by size:
4075 2580 2280 1872 885 440 412 315
181 165 147 144 56 30 30 30
30 30 30 30 30 30 30 30
30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2369 4082 4334 5452 5567 7511 7537
Size: 943 240 1713 252 1118 115 1944 26
Cuts at: 7537 7540 7601 7634 7852 9055 9583 10559 1186
Size: 3 61 33 218 1203 528 976 4623
Fragments arranged by size:
4623 1944 1713 1203 1118 976 943 528
252 240 218 115 61 33 26 3
BseYI C'CCAG_C
Cuts at: 2348 4762 5143 7117 7784 8975 8996 9030 9164
Size: 2414 381 1974 667 1191 21 34 134
Cuts at: 9164 9173 9428 13199 13695 2348
Size: 9 255 3771 496 2649
Fragments arranged by size:
3771 2649 2414 1974 1191 667 496 381
255 134 34 21 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2942 2963 3623 4142 4745 4925 5252
Size: 573 1206 21 660 519 603 180 327
Cuts at: 5252 7740 8327 9635 9870 10706 1163
Size: 2488 587 1308 235 836 4453
Fragments arranged by size:
4453 2488 1308 1206 836 660 603 587
573 519 327 235 180 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7070 7310 7494 11446 12207 13235
Size: 275 186 5069 240 184 3952 761 1028
Cuts at: 13235 1540
Size: 2301
Fragments arranged by size:
5069 3952 2301 1028 761 275 240 186
184
BsiHKAI G_wGCw'C
Cuts at: 3329 3609 4326 4356 4854 5538 5550 5904 7292
Size: 280 717 30 498 684 12 354 1388
Cuts at: 7292 7731 8214 9467 9587 9593 10947 12057 12142
Size: 439 483 1253 120 6 1354 1110 85
Cuts at: 12142 13213 3329
Size: 1071 4112
Fragments arranged by size:
4112 1388 1354 1253 1110 1071 717 684
498 483 439 354 280 120 85 30
12 6
BsmI GAATG_Cn'
Cuts at: 5210 5248 8758 11507 11600 5210
Size: 38 3510 2749 93 7606
Fragments arranged by size:
7606 3510 2749 93 38
BsmBI CGTCTCn'nnnn_
Cuts at: 4271 4989 5153 5392 6885 6942 7225 7481 4271
Size: 718 164 239 1493 57 283 256 10786
Fragments arranged by size:
10786 1493 718 283 256 239 164 57
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2764 3329 3609 4326 4356 4854 5150
Size: 399 974 565 280 717 30 498 296
Cuts at: 5150 5513 5538 5550 5904 6805 6816 7285 7292
Size: 363 25 12 354 901 11 469 7
Cuts at: 7292 7301 7380 7608 7731 8214 8944 9126 9257
Size: 9 79 228 123 483 730 182 131
Cuts at: 9257 9293 9332 9420 9455 9467 9587 9593 9803
Size: 36 39 88 35 12 120 6 210
Cuts at: 9803 10166 10947 11315 12057 12142 13213 1391
Size: 363 781 368 742 85 1071 2174
Fragments arranged by size:
2174 1071 974 901 781 742 730 717
565 498 483 469 399 368 363 363
354 296 280 228 210 182 131 123
120 88 85 79 39 36 35 30
25 12 12 11 9 7 6
BspEI T'CCGG_A
Cuts at: 13659 13659
Size: 13996
BspHI T'CATG_A
Cuts at: 2290 3832 4660 5842 8316 11885 13615 2290
Size: 1542 828 1182 2474 3569 1730 2671
Fragments arranged by size:
3569 2671 2474 1730 1542 1182 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2922 3724 4159 4653 4746 4891 5059 5274
Size: 2078 802 435 494 93 145 168 215
Cuts at: 5274 10728 11012 11351 844
Size: 5454 284 339 3489
Fragments arranged by size:
5454 3489 2078 802 494 435 339 284
215 168 145 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3322 6781 6836 7066 7113 9385
Size: 429 187 1312 3459 55 230 47 2272
Cuts at: 9385 11218 11883 1394
Size: 1833 665 3507
Fragments arranged by size:
3507 3459 2272 1833 1312 665 429 230
187 55 47
BsrDI GCAATG_nn'
Cuts at: 65 1248 2686 2911 3818 7234 12491 12665 65
Size: 1183 1438 225 907 3416 5257 174 1396
Fragments arranged by size:
5257 3416 1438 1396 1183 907 225 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2099 2952 7439 9012 9158 11159 12640
Size: 110 289 853 4487 1573 146 2001 1481
Cuts at: 12640 12827 1700
Size: 187 2869
Fragments arranged by size:
4487 2869 2001 1573 1481 853 289 187
146 110
BsrGI T'GTAC_A
Cuts at: 96 8175 96
Size: 8079 5917
BssHII G'CGCG_C
Cuts at: 1925 4893 9168 9314 9316 1925
Size: 2968 4275 146 2 6605
Fragments arranged by size:
6605 4275 2968 146 2
BssSI C'ACGA_G
Cuts at: 3629 4853 5549 7751 8156 9001 12056 13068 3629
Size: 1224 696 2202 405 845 3055 1012 4557
Fragments arranged by size:
4557 3055 2202 1224 1012 845 696 405
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2977 4723 9613 10555 11152 794
Size: 2183 1746 4890 942 597 3638
Fragments arranged by size:
4890 3638 2183 1746 942 597
BstBI TT'CG_AA
Cuts at: 2167 6508 12800 2167
Size: 4341 6292 3363
Fragments arranged by size:
6292 4341 3363
BstEII G'GTnAC_C
Cuts at: 1184 10360 10513 1184
Size: 9176 153 4667
Fragments arranged by size:
9176 4667 153
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2947 3226 4589 5474 5980 9673 10526 10804
Size: 816 279 1363 885 506 3693 853 278
Cuts at: 10804 2131
Size: 5323
Fragments arranged by size:
5323 3693 1363 885 853 816 506 279
278
BstYI r'GATC_y
Cuts at: 1972 2311 2433 4005 4462 4555 4770 5463 6874
Size: 339 122 1572 457 93 215 693 1411
Cuts at: 6874 8122 8138 8287 8790 9024 11326 11693 12078
Size: 1248 16 149 503 234 2302 367 385
Cuts at: 12078 12095 13547 13633 13645 13991 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2302 1977 1572 1452 1411 1248 693 503
457 385 367 346 339 234 215 149
122 93 86 17 16 12
BstZ17I GTA'TAC
Cuts at: 5519 5519
Size: 13996
Bsu36I CC'TnA_GG
Cuts at: 2745 10325 2745
Size: 7580 6416
BtgI C'CryG_G
Cuts at: 513 1065 2058 3135 3757 5505 7050 7251 7620
Size: 552 993 1077 622 1748 1545 201 369
Cuts at: 7620 10254 513
Size: 2634 4255
Fragments arranged by size:
4255 2634 1748 1545 1077 993 622 552
369 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 6907 7141 8832 10267 10666
Size: 683 56 899 4782 234 1691 1435 399
Cuts at: 10666 487
Size: 3817
Fragments arranged by size:
4782 3817 1691 1435 899 683 399 234
56
BtsI GCAGTG_nn'
Cuts at: 2875 4724 9702 10473 11153 11512 12306 12326 2875
Size: 1849 4978 771 680 359 794 20 4545
Fragments arranged by size:
4978 4545 1849 794 771 680 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 10462 10497 529
Size: 35 9898 35 4028
Fragments arranged by size:
9898 4028 35 35
DraI TTT'AAA
Cuts at: 6312 7905 8835 11053 11300 6312
Size: 1593 930 2218 247 9008
Fragments arranged by size:
9008 2218 1593 930 247
DraIII CAC_nnn'GTG
Cuts at: 3634 7718 3634
Size: 4084 9912
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7490 13003 809
Size: 507 6174 5513 1802
Fragments arranged by size:
6174 5513 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2121 2133
Size: 54 1425 50 275 18 234 57 12
Cuts at: 2133 3216 3238 4144 7067 7307 7470 7623 8266
Size: 1083 22 906 2923 240 163 153 643
Cuts at: 8266 8959 9597 9747 10132 10317 10470 11443 12332
Size: 693 638 150 385 185 153 973 889
Cuts at: 12332 13961 8
Size: 1629 43
Fragments arranged by size:
2923 1629 1425 1083 973 906 889 693
643 638 385 275 240 234 185 163
153 153 150 57 54 50 43 22
18 12
EagI C'GGCC_G
Cuts at: 1537 1812 7067 7307 11443 1537
Size: 275 5255 240 4136 4090
Fragments arranged by size:
5255 4136 4090 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 3807 3927 4331 4361 5357 5390 5850 5979
Size: 2454 120 404 30 996 33 460 129
Cuts at: 5979 6536 7403 7569 7681 7861 10992 11926 1353
Size: 557 867 166 112 180 3131 934 3423
Fragments arranged by size:
3423 3131 2454 996 934 867 557 460
404 180 166 129 120 112 33 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2037 5150 7682 12583 12957 13103
Size: 1064 138 448 3113 2532 4901 374 146
Cuts at: 13103 387
Size: 1280
Fragments arranged by size:
4901 3113 2532 1280 1064 448 374 146
138
EcoICRI GAG'CTC
Cuts at: 4324 4354 5536 5902 7290 7729 9465 4324
Size: 30 1182 366 1388 439 1736 8855
Fragments arranged by size:
8855 1736 1388 1182 439 366 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2699 2726 2813 2837 3026 3128
Size: 393 818 249 27 87 24 189 102
Cuts at: 3128 3302 3524 3677 4085 4219 4370 4472 4670
Size: 174 222 153 408 134 151 102 198
Cuts at: 4670 4733 4826 5216 5237 5497 5736 5765 6386
Size: 63 93 390 21 260 239 29 621
Cuts at: 6386 6681 6815 6827 7082 7150 7207 7247 7631
Size: 295 134 12 255 68 57 40 384
Cuts at: 7631 7793 8357 8699 9269 9373 9481 9515 10256
Size: 162 564 342 570 104 108 34 741
Cuts at: 10256 10529 10689 11123 12059 12656 13443 13711 1239
Size: 273 160 434 936 597 787 268 1524
Fragments arranged by size:
1524 936 818 787 741 621 597 570
564 434 408 393 390 384 342 295
273 268 260 255 249 239 222 198
189 174 162 160 153 151 134 134
108 104 102 102 93 87 68 63
57 40 34 29 27 24 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 5096 9443 10223 10694 11096 11099 5096
Size: 4347 780 471 402 3 7993
Fragments arranged by size:
7993 4347 780 471 402 3
EcoO109I rG'GnC_Cy
Cuts at: 2128 2258 2812 2824 3439 4126 4201 4642 5721
Size: 130 554 12 615 687 75 441 1079
Cuts at: 5721 6210 6490 7281 7298 7516 7963 8940 8941
Size: 489 280 791 17 218 447 977 1
Cuts at: 8941 9123 10162 10163 10330 11104 2128
Size: 182 1039 1 167 774 5020
Fragments arranged by size:
5020 1079 1039 977 791 774 687 615
554 489 447 441 280 218 182 167
130 75 17 12 1 1
EcoRI G'AATT_C
Cuts at: 10061 10874 11305 11683 10061
Size: 813 431 378 12374
Fragments arranged by size:
12374 813 431 378
EcoRV GAT'ATC
Cuts at: 5791 5791
Size: 13996
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 8638 9436 2389
Size: 6249 798 6949
Fragments arranged by size:
6949 6249 798
FseI GG_CCGG'CC
Cuts at: 9162 9162
Size: 13996
FspI TGC'GCA
Cuts at: 4370 7325 12502 4370
Size: 2955 5177 5864
Fragments arranged by size:
5864 5177 2955
HaeII r_GCGC'y
Cuts at: 1678 4141 4486 7434 8994 9270 13143 1678
Size: 2463 345 2948 1560 276 3873 2531
Fragments arranged by size:
3873 2948 2531 2463 1560 345 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3259 3291 3329 3361 3410
Size: 32 135 32 1687 32 38 32 49
Cuts at: 3410 3442 3506 3538 4292 4324 4385 4397 4417
Size: 32 64 32 754 32 61 12 20
Cuts at: 4417 4429 5002 5034 5761 5793 6447 6479 6950
Size: 12 573 32 727 32 654 32 471
Cuts at: 6950 6982 7199 7231 7427 7459 7658 7690 9086
Size: 32 217 32 196 32 199 32 1396
Cuts at: 9086 9118 9509 9541 10110 10142 10413 10445 12637
Size: 32 391 32 569 32 271 32 2192
Cuts at: 12637 12669 12711 12743 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2192 1687 1396 754 727 654 573
569 471 391 271 217 199 196 135
64 61 49 42 38 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4180 5035 7801 9891 11340 11586
Size: 988 281 2242 855 2766 2090 1449 246
Cuts at: 11586 669
Size: 3079
Fragments arranged by size:
3079 2766 2242 2090 1449 988 855 281
246
HindIII A'AGCT_T
Cuts at: 748 2875 3402 10022 748
Size: 2127 527 6620 4722
Fragments arranged by size:
6620 4722 2127 527
HpaI GTT'AAC
Cuts at: 11586 11586
Size: 13996
KasI G'GCGC_C
Cuts at: 8990 9266 8990
Size: 276 13720
KpnI G_GTAC'C
Cuts at: 10720 11321 10720
Size: 601 13395
MfeI C'AATT_G
Cuts at: 7740 11595 7740
Size: 3855 10141
MluI A'CGCG_T
Cuts at: 13872 13872
Size: 13996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4748 5380 5784 6091 7060 7482 9064 10918
Size: 3443 632 404 307 969 422 1582 1854
Cuts at: 10918 13110 13294 1305
Size: 2192 184 2007
Fragments arranged by size:
3443 2192 2007 1854 1582 969 632 422
404 307 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3218 3240 4146 7625 8268
Size: 54 1425 343 1386 22 906 3479 643
Cuts at: 8268 9749 10472 10
Size: 1481 723 3534
Fragments arranged by size:
3534 3479 1481 1425 1386 906 723 643
343 54 22
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2690 3129 3212 3786 4022 4038
Size: 648 1299 225 439 83 574 236 16
Cuts at: 4038 4040 4952 5472 5640 7181 9674 9695 9846
Size: 2 912 520 168 1541 2493 21 151
Cuts at: 9846 11232 11954 12313 12472 518
Size: 1386 722 359 159 2042
Fragments arranged by size:
2493 2042 1541 1386 1299 912 722 648
574 520 439 359 236 225 168 159
151 83 21 16 2
MspA1I CmG'CkG
Cuts at: 1606 2019 2060 2791 3028 3280 3544 3649 3655
Size: 413 41 731 237 252 264 105 6
Cuts at: 3655 3706 3904 3958 4294 4366 4729 5608 6190
Size: 51 198 54 336 72 363 879 582
Cuts at: 6190 7121 7362 7527 8329 8877 8901 8910 8928
Size: 931 241 165 802 548 24 9 18
Cuts at: 8928 8958 8982 9373 9535 9809 11412 12089 13237
Size: 30 24 391 162 274 1603 677 1148
Cuts at: 13237 13482 1606
Size: 245 2120
Fragments arranged by size:
2120 1603 1148 931 879 802 731 677
582 548 413 391 363 336 274 264
252 245 241 237 198 165 162 105
72 54 51 41 30 24 24 18
9 6
NaeI GCC'GGC
Cuts at: 2101 7441 9014 9160 11161 2101
Size: 5340 1573 146 2001 4936
Fragments arranged by size:
5340 4936 2001 1573 146
NarI GG'CG_CC
Cuts at: 8991 9267 8991
Size: 276 13720
NcoI C'CATG_G
Cuts at: 513 1065 3135 7620 513
Size: 552 2070 4485 6889
Fragments arranged by size:
6889 4485 2070 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13996
NgoMIV G'CCGG_C
Cuts at: 2099 7439 9012 9158 11159 2099
Size: 5340 1573 146 2001 4936
Fragments arranged by size:
5340 4936 2001 1573 146
NheI G'CTAG_C
Cuts at: 1052 9320 1052
Size: 8268 5728
NotI GC'GGCC_GC
Cuts at: 7067 11443 7067
Size: 4376 9620
NruI TCG'CGA
Cuts at: 3272 3272
Size: 13996
NspI r_CATG'y
Cuts at: 1132 1229 2395 2695 2996 3343 4021 4871 5290
Size: 97 1166 300 301 347 678 850 419
Cuts at: 5290 5639 6037 6206 6421 6647 8176 9769 11354
Size: 349 398 169 215 226 1529 1593 1585
Cuts at: 11354 12899 1132
Size: 1545 2229
Fragments arranged by size:
2229 1593 1585 1545 1529 1166 850 678
419 398 349 347 301 300 226 215
169 97
PasI CC'CwG_GG
Cuts at: 1348 5740 8485 1348
Size: 4392 2745 6859
Fragments arranged by size:
6859 4392 2745
PciI A'CATG_T
Cuts at: 3339 4017 6643 8172 9765 12895 3339
Size: 678 2626 1529 1593 3130 4440
Fragments arranged by size:
4440 3130 2626 1593 1529 678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3616 3958 7051 10861 1108
Size: 2508 342 3093 3810 4243
Fragments arranged by size:
4243 3810 3093 2508 342
PfoI T'CCnGG_A
Cuts at: 3615 4329 4638 4959 5937 6774 9020 10635 10980
Size: 714 309 321 978 837 2246 1615 345
Cuts at: 10980 11139 3615
Size: 159 6472
Fragments arranged by size:
6472 2246 1615 978 837 714 345 321
309 159
PmeI GTTT'AAAC
Cuts at: 11300 11300
Size: 13996
PmlI CAC'GTG
Cuts at: 5170 7381 9588 10089 10278 5170
Size: 2211 2207 501 189 8888
Fragments arranged by size:
8888 2211 2207 501 189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 6513 6545 9720 9752 10809 10841 10895
Size: 32 3599 32 3175 32 1057 32 54
Cuts at: 10895 10927 12065 12097 13578 13610 2882
Size: 32 1138 32 1481 32 3268
Fragments arranged by size:
3599 3268 3175 1481 1138 1057 54 32
32 32 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2258 2824 3439 4126 4201 4642 5721 6210
Size: 130 566 615 687 75 441 1079 489
Cuts at: 6210 6490 7963 10330 11104 2128
Size: 280 1473 2367 774 5020
Fragments arranged by size:
5020 2367 1473 1079 774 687 615 566
489 441 280 130 75
PshAI GACnn'nnGTC
Cuts at: 1613 5412 7089 7236 10626 1613
Size: 3799 1677 147 3390 4983
Fragments arranged by size:
4983 3799 3390 1677 147
PsiI TTA'TAA
Cuts at: 6603 11566 6603
Size: 4963 9033
PspOMI G'GGCC_C
Cuts at: 5146 7281 7297 8940 9122 10162 5146
Size: 2135 16 1643 182 1040 8980
Fragments arranged by size:
8980 2135 1643 1040 182 16
PspXI vC'TCGA_Gb
Cuts at: 4409 9076 4409
Size: 4667 9329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6450 6482 6450
Size: 32 13964
PstI C_TGCA'G
Cuts at: 830 2442 2919 3438 3705 4077 4170 4290 4365
Size: 1612 477 519 267 372 93 120 75
Cuts at: 4365 4566 4743 4902 5078 8555 9073 9444 10539
Size: 201 177 159 176 3477 518 371 1095
Cuts at: 10539 10733 11100 11157 11274 11348 830
Size: 194 367 57 117 74 3478
Fragments arranged by size:
3478 3477 1612 1095 519 518 477 372
371 367 267 201 194 177 176 159
120 117 93 75 74 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13996
PvuII CAG'CTG
Cuts at: 2791 3028 3280 3655 3706 3904 3958 4294 4366
Size: 237 252 375 51 198 54 336 72
Cuts at: 4366 4729 5608 6190 8329 8982 9809 2791
Size: 363 879 582 2139 653 827 6978
Fragments arranged by size:
6978 2139 879 827 653 582 375 363
336 252 237 198 72 54 51
SacI G_AGCT'C
Cuts at: 4326 4356 5538 5904 7292 7731 9467 4326
Size: 30 1182 366 1388 439 1736 8855
Fragments arranged by size:
8855 1736 1388 1182 439 366 30
SacII CC_GC'GG
Cuts at: 2061 2061
Size: 13996
SalI G'TCGA_C
Cuts at: 1936 11338 1936
Size: 9402 4594
SanDI GG'GwC_CC
Cuts at: 6490 6490
Size: 13996
SapI GCTCTTCn'nnn_
Cuts at: 4331 4361 7403 4331
Size: 30 3042 10924
Fragments arranged by size:
10924 3042 30
SbfI CC_TGCA'GG
Cuts at: 2442 4170 4743 11100 11348 2442
Size: 1728 573 6357 248 5090
Fragments arranged by size:
6357 5090 1728 573 248
ScaI AGT'ACT
Cuts at: 12244 13956 12244
Size: 1712 12284
SexAI A'CCwGG_T
Cuts at: 10509 10509
Size: 13996
SfcI C'TryA_G
Cuts at: 826 1045 2438 2915 3434 3701 4073 4166 4286
Size: 219 1393 477 519 267 372 93 120
Cuts at: 4286 4361 4562 4739 4898 4928 5074 6467 8551
Size: 75 201 177 159 30 146 1393 2084
Cuts at: 8551 9069 9440 10198 10210 10390 10535 10729 11096
Size: 518 371 758 12 180 145 194 367
Cuts at: 11096 11153 11270 11344 12479 13160 13351 826
Size: 57 117 74 1135 681 191 1471
Fragments arranged by size:
2084 1471 1393 1393 1135 758 681 519
518 477 372 371 367 267 219 201
194 191 180 177 159 146 145 120
117 93 75 74 57 30 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 9155 9155
Size: 13996
SfoI GGC'GCC
Cuts at: 8992 9268 8992
Size: 276 13720
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13996
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13996
SmaI CCC'GGG
Cuts at: 1872 9121 9353 9513 11323 1872
Size: 7249 232 160 1810 4545
Fragments arranged by size:
7249 4545 1810 232 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4409 5006 5024 5084 6716 8348
Size: 197 926 2466 597 18 60 1632 1632
Cuts at: 8348 9076 9881 12100 13001 13263 820
Size: 728 805 2219 901 262 1553
Fragments arranged by size:
2466 2219 1632 1632 1553 926 901 805
728 597 262 197 60 18
SnaBI TAC'GTA
Cuts at: 493 5955 10954 11787 493
Size: 5462 4999 833 2702
Fragments arranged by size:
5462 4999 2702 833
SpeI A'CTAG_T
Cuts at: 152 7933 152
Size: 7781 6215
SphI G_CATG'C
Cuts at: 1132 4871 5639 6421 11354 1132
Size: 3739 768 782 4933 3774
Fragments arranged by size:
4933 3774 3739 782 768
SspI AAT'ATT
Cuts at: 5 52 11920 5
Size: 47 11868 2081
Fragments arranged by size:
11868 2081 47
StuI AGG'CCT
Cuts at: 1517 2568 3087 4586 7131 7673 1517
Size: 1051 519 1499 2545 542 7840
Fragments arranged by size:
7840 2545 1499 1051 542 519
StyI C'CwwG_G
Cuts at: 513 1065 2124 2931 3135 3597 4546 6886 7620
Size: 552 1059 807 204 462 949 2340 734
Cuts at: 7620 7966 9306 9724 10333 11073 11435 513
Size: 346 1340 418 609 740 362 3074
Fragments arranged by size:
3074 2340 1340 1059 949 807 740 734
609 552 462 418 362 346 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 4052 5543 6620 6667 7633 9638
Size: 1255 1122 1350 1491 1077 47 966 2005
Cuts at: 9638 10532 12023 12040 12193 12378 12823 325
Size: 894 1491 17 153 185 445 1498
Fragments arranged by size:
2005 1498 1491 1491 1350 1255 1122 1077
966 894 445 185 153 47 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5103 5715 8175
Size: 274 80 33 51 1443 3126 612 2460
Cuts at: 8175 12242 13954 96
Size: 4067 1712 138
Fragments arranged by size:
4067 3126 2460 1712 1443 612 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3380 3412 4244 4276 4589 4621 8393
Size: 32 73 32 832 32 313 32 3772
Cuts at: 8393 8425 11069 11101 13956 13988 3275
Size: 32 2644 32 2855 32 3283
Fragments arranged by size:
3772 3283 2855 2644 832 313 73 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 10511 1316
Size: 9195 4801
XbaI T'CTAG_A
Cuts at: 11332 11332
Size: 13996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2493 2824 3226 5452 7174 7876 9557 10465 10600
Size: 331 402 2226 1722 702 1681 908 135
Cuts at: 10600 2493
Size: 5889
Fragments arranged by size:
5889 2226 1722 1681 908 702 402 331
135
XhoI C'TCGA_G
Cuts at: 1943 4409 5084 9076 1943
Size: 2466 675 3992 6863
Fragments arranged by size:
6863 3992 2466 675
XmaI C'CCGG_G
Cuts at: 1870 9119 9351 9511 11321 1870
Size: 7249 232 160 1810 4545
Fragments arranged by size:
7249 4545 1810 232 160
XmnI GAAnn'nnTTC
Cuts at: 2877 3768 4448 5885 5988 8342 8621 12125 2877
Size: 891 680 1437 103 2354 279 3504 4748
Fragments arranged by size:
4748 3504 2354 1437 891 680 279 103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4537 5251 6936 11804
Size: 53 83 186 936 3003 714 1685 4868
Cuts at: 11804 276
Size: 2468
Fragments arranged by size:
4868 3003 2468 1685 936 714 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BplI BsiWI FspAI NsiI PacI
RsrII SrfI SwaI