Restriction map for
pFN21AE1495
(Circular) (Six-base) MAPSORT of: pFN21AE1495.seq Check: 1663 from: 1 to: 5428
pFN21AE1495 5428 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2729 3491 4875 5143 1632
Size: 1097 762 1384 268 1917
Fragments arranged by size:
1917 1384 1097 762 268
AfeI AGC'GCT
Cuts at: 2390 2390
Size: 5428
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 2309 2438 1166
Size: 1143 129 4156
Fragments arranged by size:
4156 1143 129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2239 2271 2239
Size: 32 5396
AlwNI CAG_nnn'CTG
Cuts at: 3171 4743 3171
Size: 1572 3856
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2678 2737 2963 3115 5224 1493
Size: 1185 59 226 152 2109 1697
Fragments arranged by size:
2109 1697 1185 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2581 2622 2753 1870
Size: 73 138 500 41 131 4545
Fragments arranged by size:
4545 500 138 131 73 41
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 2749 4204 618
Size: 325 217 627 541 421 1455 1842
Fragments arranged by size:
1842 1455 627 541 421 325 217
BanII G_rGCy'C
Cuts at: 1391 2747 1391
Size: 1356 4072
BbeI G_GCGC'C
Cuts at: 2332 2332
Size: 5428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2529 928
Size: 971 630 3827
Fragments arranged by size:
3827 971 630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2319 5423 2319
Size: 3104 2324
BlpI GC'TnA_GC
Cuts at: 2271 2845 2271
Size: 574 4854
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4645 1790
Size: 1699 1156 2573
Fragments arranged by size:
2573 1699 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 4117 442
Size: 416 53 83 399 24 1742 958 1753
Fragments arranged by size:
1753 1742 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2250 2250
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2243 4088 1239
Size: 993 11 1845 2579
Fragments arranged by size:
2579 1845 993 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2148 2708 4091 882
Size: 617 649 560 1383 2219
Fragments arranged by size:
2219 1383 649 617 560
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2329 3235 3617 4252
Size: 53 83 186 936 796 906 382 635
Cuts at: 4252 275
Size: 1451
Fragments arranged by size:
1451 936 906 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2673 3861 4259 4533 4680 5091 2673
Size: 1188 398 274 147 411 3010
Fragments arranged by size:
3010 1188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2239 2269 1178
Size: 30 165 30 836 30 4337
Fragments arranged by size:
4337 836 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 1186
Size: 913 4515
BseYI C'CCAG_C
Cuts at: 2016 4631 5127 2016
Size: 2615 496 2317
Fragments arranged by size:
2615 2317 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2405 2441 2462 2544 1163
Size: 573 396 273 36 21 82 4047
Fragments arranged by size:
4047 573 396 273 82 36 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2878 3639 4667 1540
Size: 275 186 877 761 1028 2301
Fragments arranged by size:
2301 1028 877 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3489 3574 4645 3489
Size: 85 1071 4272
Fragments arranged by size:
4272 1071 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2747 3489 3574 4645 1391
Size: 399 957 742 85 1071 2174
Fragments arranged by size:
2174 1071 957 742 399 85
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 2783 844
Size: 1211 728 3489
Fragments arranged by size:
3489 1211 728
BsrBI CCG'CTC
Cuts at: 1394 1823 2121 2202 3315 1394
Size: 429 298 81 1113 3507
Fragments arranged by size:
3507 1113 429 298 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 3923 4097 65
Size: 1183 1017 1658 174 1396
Fragments arranged by size:
1658 1396 1183 1017 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2275 2652 4072 4259 1700
Size: 110 465 377 1420 187 2869
Fragments arranged by size:
2869 1420 465 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 2235 3488 4500 2235
Size: 1253 1012 3163
Fragments arranged by size:
3163 1253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 2626 1184
Size: 1442 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2359
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2319 2758 3125 3510 3527 4979 5065 5077
Size: 347 439 367 385 17 1452 86 12
Cuts at: 5077 5423 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 439 385 367 347 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2508 513
Size: 552 1443 3433
Fragments arranged by size:
3433 1443 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2429 2944 3738 3758 2429
Size: 515 794 20 4099
Fragments arranged by size:
4099 794 515 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2366 2875
Size: 54 1425 50 275 18 447 89 509
Cuts at: 2875 3764 5393 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 509 447 275 89 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 3358 1353
Size: 677 1328 3423
Fragments arranged by size:
3423 1328 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2639 4015 4389 4535 387
Size: 1064 138 1050 1376 374 146 1280
Fragments arranged by size:
1376 1280 1064 1050 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2243 2729 3491 4088 4875 5143
Size: 393 600 11 486 762 597 787 268
Cuts at: 5143 1239
Size: 1524
Fragments arranged by size:
1524 787 762 600 597 486 393 268
11
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 5428
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 5428
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2024 2064 2332 2392 4575 1678
Size: 346 40 268 60 2183 2531
Fragments arranged by size:
2531 2183 346 268 60 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4069 4101 4143 4175 1373
Size: 32 135 32 2497 32 42 32 2626
Fragments arranged by size:
2626 2497 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KasI G'GCGC_C
Cuts at: 2328 2328
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2161 2163 4542 4726 1305
Size: 856 2 2379 184 2007
Fragments arranged by size:
2379 2007 856 184 2
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 2438 2539 2691 3386 3745 3904
Size: 648 1143 129 101 152 695 359 159
Cuts at: 3904 518
Size: 2042
Fragments arranged by size:
2042 1143 695 648 359 159 152 129
101
MspA1I CmG'CkG
Cuts at: 1606 2446 2558 2844 3521 4669 4914 1606
Size: 840 112 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 840 677 286 245 112
NaeI GCC'GGC
Cuts at: 2277 2654 2277
Size: 377 5051
NarI GG'CG_CC
Cuts at: 2329 2329
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NgoMIV G'CCGG_C
Cuts at: 2275 2652 2275
Size: 377 5051
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2538 2786 4331 1132
Size: 97 1309 248 1545 2229
Fragments arranged by size:
2229 1545 1309 248 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2239 2271 3497 3529 5010 5042 2239
Size: 32 1226 32 1481 32 2625
Fragments arranged by size:
2625 1481 1226 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 5428
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2780 830
Size: 1950 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2446 2558 2446
Size: 112 5316
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SanDI GG'GwC_CC
Cuts at: 2071 2071
Size: 5428
SapI GCTCTTCn'nnn_
Cuts at: 2030 2030
Size: 5428
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2776 3911 4592 4783 826
Size: 219 1731 1135 681 191 1471
Fragments arranged by size:
1731 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2330 2330
Size: 5428
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2583 2755 1872
Size: 711 172 4545
Fragments arranged by size:
4545 711 172
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3532 4433 4695 820
Size: 197 926 1589 901 262 1553
Fragments arranged by size:
1589 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2538 2786 1132
Size: 1406 248 3774
Fragments arranged by size:
3774 1406 248
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2661 2719 2867 513
Size: 552 1596 58 148 3074
Fragments arranged by size:
3074 1596 552 148 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 3455 3472 3625 3810 4255 325
Size: 1255 870 1005 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1005 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5388 5420 5388
Size: 32 5396
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2515 2515
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2581 2753 1870
Size: 711 172 4545
Fragments arranged by size:
4545 711 172
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI
SwaI