Restriction map for
pFN21AE1492
(Circular) (Six-base) MAPSORT of: pFN21AE1492.seq Check: 3670 from: 1 to: 7243
pFN21AE1492 7243 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5053 278
Size: 53 83 186 936 3517 2468
Fragments arranged by size:
3517 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4586 1937
Size: 2649 4594
Acc65I G'GTAC_C
Cuts at: 2803 4564 2803
Size: 1761 5482
AclI AA'CG_TT
Cuts at: 1563 5370 5743 1563
Size: 3807 373 3063
Fragments arranged by size:
3807 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2028 3366 3393 5306 6690 6958 1632
Size: 396 1338 27 1913 1384 268 1917
Fragments arranged by size:
1917 1913 1384 1338 396 268 27
AflII C'TTAA_G
Cuts at: 820 1017 2306 820
Size: 197 1289 5757
Fragments arranged by size:
5757 1289 197
AflIII A'CryG_T
Cuts at: 1203 2655 3083 6142 7119 1203
Size: 1452 428 3059 977 1327
Fragments arranged by size:
3059 1452 1327 977 428
AgeI A'CCGG_T
Cuts at: 6074 6074
Size: 7243
AhdI GACnn_n'nnGTC
Cuts at: 2683 3991 5972 2683
Size: 1308 1981 3954
Fragments arranged by size:
3954 1981 1308
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2734 3908 4359 1184
Size: 1550 1174 451 4068
Fragments arranged by size:
4068 1550 1174 451
AlwNI CAG_nnn'CTG
Cuts at: 3038 3577 4986 6558 3038
Size: 539 1409 1572 3723
Fragments arranged by size:
3723 1572 1409 539
ApaLI G'TGCA_C
Cuts at: 5300 6456 5300
Size: 1156 6087
ApoI r'AATT_y
Cuts at: 1493 2186 3449 3779 4046 4552 4778 4930 7039
Size: 693 1263 330 267 506 226 152 2109
Cuts at: 7039 1493
Size: 1697
Fragments arranged by size:
2109 1697 1263 693 506 330 267 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5797 160
Size: 872 4765 1606
Fragments arranged by size:
4765 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
AvaI C'yCGr_G
Cuts at: 1870 1943 2349 4568 1870
Size: 73 406 2219 4545
Fragments arranged by size:
4545 2219 406 73
AvrII C'CTAG_G
Cuts at: 3434 3434
Size: 7243
BamHI G'GATC_C
Cuts at: 4573 4940 4573
Size: 367 6876
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 4564 6019 618
Size: 325 217 627 1016 1761 1455 1842
Fragments arranged by size:
1842 1761 1455 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3875 4386 4562 1391
Size: 2484 511 176 4072
Fragments arranged by size:
4072 2484 511 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3332 928
Size: 971 1433 4839
Fragments arranged by size:
4839 1433 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2306 2340 5434 5468 1903
Size: 34 369 34 3094 34 3678
Fragments arranged by size:
3678 3094 369 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2086 5135 6345 2004
Size: 82 3049 1210 2902
Fragments arranged by size:
3049 2902 1210 82
BclI T'GATC_A
Cuts at: 1548 2590 3715 1548
Size: 1042 1125 5076
Fragments arranged by size:
5076 1125 1042
BfrBI ATG'CAT
Cuts at: 4070 4070
Size: 7243
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5854 136
Size: 107 122 71 631 605 4182 1525
Fragments arranged by size:
4182 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2273 7238 2273
Size: 4965 2278
BlpI GC'TnA_GC
Cuts at: 4301 4660 4301
Size: 359 6884
Bme1580I G_kGCm'C
Cuts at: 1790 5304 6460 1790
Size: 3514 1156 2573
Fragments arranged by size:
3514 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7243
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4445 4974 5932
Size: 416 53 83 399 24 3028 529 958
Cuts at: 5932 442
Size: 1753
Fragments arranged by size:
3028 1753 958 529 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3778 3894 3778
Size: 116 7127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3861 4461 5903 1239
Size: 2622 600 1442 2579
Fragments arranged by size:
2622 2579 1442 600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2771 5368 6233 6531 2771
Size: 2597 865 298 3483
Fragments arranged by size:
3483 2597 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2424 3627 5906 882
Size: 617 925 1203 2279 2219
Fragments arranged by size:
2279 2219 1203 925 617
BsaAI yAC'GTr
Cuts at: 493 1204 3674 5034 493
Size: 711 2470 1360 2702
Fragments arranged by size:
2702 2470 1360 711
BsaBI GATnn'nnATC
Cuts at: 2795 2919 7237 2795
Size: 124 4318 2801
Fragments arranged by size:
4318 2801 124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5050 5432 6067 275
Size: 53 83 186 936 3517 382 635 1451
Fragments arranged by size:
3517 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5676 6074 6348 6495 6906 5676
Size: 398 274 147 411 6013
Fragments arranged by size:
6013 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4145 4175 1178
Size: 30 165 30 2742 30 4246
Fragments arranged by size:
4246 2742 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3989 1186
Size: 2803 4440
BseYI C'CCAG_C
Cuts at: 2942 6446 6942 2942
Size: 3504 496 3243
Fragments arranged by size:
3504 3243 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 3291 3510 1163
Size: 573 896 659 219 4896
Fragments arranged by size:
4896 896 659 573 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4693 5454 6482 1540
Size: 275 186 2692 761 1028 2301
Fragments arranged by size:
2692 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4091 4386 5304 5389 6460 4091
Size: 295 918 85 1071 4874
Fragments arranged by size:
4874 1071 918 295 85
BsmI GAATG_Cn'
Cuts at: 4754 4847 4754
Size: 93 7150
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3875 4091 4386 4562 5304 5389 6460
Size: 399 2085 216 295 176 742 85 1071
Cuts at: 6460 1391
Size: 2174
Fragments arranged by size:
2174 2085 1071 742 399 295 216 176
85
BspEI T'CCGG_A
Cuts at: 6906 6906
Size: 7243
BspHI T'CATG_A
Cuts at: 5132 6862 5132
Size: 1730 5513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 3507 4291 4598 844
Size: 1224 1439 784 307 3489
Fragments arranged by size:
3489 1439 1224 784 307
BsrBI CCG'CTC
Cuts at: 1394 1823 5130 1394
Size: 429 3307 3507
Fragments arranged by size:
3507 3307 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3890 5738 5912 65
Size: 1183 2642 1848 174 1396
Fragments arranged by size:
2642 1848 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 5887 6074 1700
Size: 110 2502 1575 187 2869
Fragments arranged by size:
2869 2502 1575 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7243
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7243
BssSI C'ACGA_G
Cuts at: 2438 5303 6315 2438
Size: 2865 1012 3366
Fragments arranged by size:
3366 2865 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7243
BstBI TT'CG_AA
Cuts at: 6047 6047
Size: 7243
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7243
BstXI CCAn_nnnn'nTGG
Cuts at: 4040 4040
Size: 7243
BstYI r'GATC_y
Cuts at: 1972 2273 2521 4573 4940 5325 5342 6794 6880
Size: 301 248 2052 367 385 17 1452 86
Cuts at: 6880 6892 7238 1972
Size: 12 346 1977
Fragments arranged by size:
2052 1977 1452 385 367 346 301 248
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2388 513
Size: 552 939 384 5368
Fragments arranged by size:
5368 939 552 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6504
Fragments arranged by size:
6504 683 56
BtsI GCAGTG_nn'
Cuts at: 2690 3036 4052 4759 5553 5573 2690
Size: 346 1016 707 794 20 4360
Fragments arranged by size:
4360 1016 794 707 346 20
ClaI AT'CG_AT
Cuts at: 1554 3018 1554
Size: 1464 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3174 3209 3789 3824 529
Size: 35 2610 35 580 35 3948
Fragments arranged by size:
3948 2610 580 35 35 35
DraI TTT'AAA
Cuts at: 2556 4405 4547 2556
Size: 1849 142 5252
Fragments arranged by size:
5252 1849 142
DraIII CAC_nnn'GTG
Cuts at: 2696 2696
Size: 7243
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6250 809
Size: 507 4934 1802
Fragments arranged by size:
4934 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4516 4690 5579
Size: 54 1425 50 275 18 2686 174 889
Cuts at: 5579 7208 8
Size: 1629 43
Fragments arranged by size:
2686 1629 1425 889 275 174 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4690 1537
Size: 275 2878 4090
Fragments arranged by size:
4090 2878 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2275 2634 2976 3747 4322 4482 5173 1353
Size: 922 359 342 771 575 160 691 3423
Fragments arranged by size:
3423 922 771 691 575 359 342 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5830 6204 6350 387
Size: 1064 138 4241 374 146 1280
Fragments arranged by size:
4241 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4384 4384
Size: 7243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 3366 3393 3861 4461 5306 5903
Size: 393 396 1338 27 468 600 845 597
Cuts at: 5903 6690 6958 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1338 845 787 600 597 468 396
393 268 27
EcoO109I rG'GnC_Cy
Cuts at: 4280 4280
Size: 7243
EcoRI G'AATT_C
Cuts at: 4046 4552 4930 4046
Size: 506 378 6359
Fragments arranged by size:
6359 506 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2318 2318
Size: 7243
FspI TGC'GCA
Cuts at: 2463 5749 2463
Size: 3286 3957
HaeII r_GCGC'y
Cuts at: 1678 6390 1678
Size: 4712 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 2769 2801 3536
Size: 32 135 32 1097 32 68 32 735
Cuts at: 3536 3568 3740 3772 3977 4009 4145 4177 4258
Size: 32 172 32 205 32 136 32 81
Cuts at: 4258 4290 4321 4353 4449 4481 5884 5916 5958
Size: 32 31 32 96 32 1403 32 42
Cuts at: 5958 5990 1373
Size: 32 2626
Fragments arranged by size:
2626 1403 1097 735 205 172 136 135
96 81 68 42 32 32 32 32
32 32 32 32 32 32 32 32
32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 3658 4587 4833 669
Size: 988 281 1720 929 246 3079
Fragments arranged by size:
3079 1720 988 929 281 246
HindIII A'AGCT_T
Cuts at: 748 2040 4076 4421 748
Size: 1292 2036 345 3570
Fragments arranged by size:
3570 2036 1292 345
HpaI GTT'AAC
Cuts at: 4833 4833
Size: 7243
KpnI G_GTAC'C
Cuts at: 2807 4568 2807
Size: 1761 5482
MfeI C'AATT_G
Cuts at: 3170 4842 3170
Size: 1672 5571
MluI A'CGCG_T
Cuts at: 7119 7119
Size: 7243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4492 6357 6541 1305
Size: 3187 1865 184 2007
Fragments arranged by size:
3187 2007 1865 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5421
Fragments arranged by size:
5421 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 3041 4341 5201 5560 5719 518
Size: 648 891 984 1300 860 359 159 2042
Fragments arranged by size:
2042 1300 984 891 860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4659 5336 6484 6729 1606
Size: 3053 677 1148 245 2120
Fragments arranged by size:
3053 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 4314 4314
Size: 7243
NcoI C'CATG_G
Cuts at: 513 1065 2004 2388 513
Size: 552 939 384 5368
Fragments arranged by size:
5368 939 552 384
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7243
NgoMIV G'CCGG_C
Cuts at: 4312 4312
Size: 7243
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7243
NotI GC'GGCC_GC
Cuts at: 4690 4690
Size: 7243
NsiI A_TGCA'T
Cuts at: 4072 4072
Size: 7243
NspI r_CATG'y
Cuts at: 1132 1229 2387 2659 2721 3087 4020 4601 6146
Size: 97 1158 272 62 366 933 581 1545
Cuts at: 6146 1132
Size: 2229
Fragments arranged by size:
2229 1545 1158 933 581 366 272 97
62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7243
PciI A'CATG_T
Cuts at: 2655 3083 6142 2655
Size: 428 3059 3756
Fragments arranged by size:
3756 3059 428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3577 1108
Size: 2469 4774
PmeI GTTT'AAAC
Cuts at: 4547 4547
Size: 7243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5312 5344 6825 6857 5312
Size: 32 1481 32 5698
Fragments arranged by size:
5698 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4280 4280
Size: 7243
PshAI GACnn'nnGTC
Cuts at: 1613 3142 1613
Size: 1529 5714
PsiI TTA'TAA
Cuts at: 4813 4813
Size: 7243
PstI C_TGCA'G
Cuts at: 830 4059 4246 4595 830
Size: 3229 187 349 3478
Fragments arranged by size:
3478 3229 349 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7243
SacI G_AGCT'C
Cuts at: 4386 4386
Size: 7243
SalI G'TCGA_C
Cuts at: 1936 4585 1936
Size: 2649 4594
SapI GCTCTTCn'nnn_
Cuts at: 2976 2976
Size: 7243
SbfI CC_TGCA'GG
Cuts at: 4595 4595
Size: 7243
ScaI AGT'ACT
Cuts at: 3190 3217 5491 7203 3190
Size: 27 2274 1712 3230
Fragments arranged by size:
3230 2274 1712 27
SexAI A'CCwGG_T
Cuts at: 3390 3390
Size: 7243
SfcI C'TryA_G
Cuts at: 826 1045 4055 4242 4591 5726 6407 6598 826
Size: 219 3010 187 349 1135 681 191 1471
Fragments arranged by size:
3010 1471 1135 681 349 219 191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7243
SmaI CCC'GGG
Cuts at: 1872 4570 1872
Size: 2698 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2349 2786 5347 6248 6510
Size: 197 926 363 43 437 2561 901 262
Cuts at: 6510 820
Size: 1553
Fragments arranged by size:
2561 1553 926 901 437 363 262 197
43
SnaBI TAC'GTA
Cuts at: 493 5034 493
Size: 4541 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7243
SphI G_CATG'C
Cuts at: 1132 2721 4601 1132
Size: 1589 1880 3774
Fragments arranged by size:
3774 1880 1589
SspI AAT'ATT
Cuts at: 5 52 4129 4536 5167 5
Size: 47 4077 407 631 2081
Fragments arranged by size:
4077 2081 631 407 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7243
StyI C'CwwG_G
Cuts at: 513 1065 2004 2388 3236 3434 4682 513
Size: 552 939 384 848 198 1248 3074
Fragments arranged by size:
3074 1248 939 848 552 384 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3817 5270 5287 5440 5625 6070 325
Size: 1255 2237 1453 17 153 185 445 1498
Fragments arranged by size:
2237 1498 1453 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2243 3188 3215
Size: 274 80 33 51 1443 266 945 27
Cuts at: 3215 5489 7201 96
Size: 2274 1712 138
Fragments arranged by size:
2274 1712 1443 945 274 266 138 80
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7203 7235 7203
Size: 32 7211
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7243
XbaI T'CTAG_A
Cuts at: 2276 4579 2276
Size: 2303 4940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 2573
Size: 7243
XhoI C'TCGA_G
Cuts at: 1943 2349 1943
Size: 406 6837
XmaI C'CCGG_G
Cuts at: 1870 4568 1870
Size: 2698 4545
XmnI GAAnn'nnTTC
Cuts at: 4422 5372 4422
Size: 950 6293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5051 276
Size: 53 83 186 936 3517 2468
Fragments arranged by size:
3517 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI
SrfI SwaI