Restriction map for
pFN21AE1487
(Circular) (Six-base) MAPSORT of: pFN21AE1487.seq Check: 3858 from: 1 to: 5200
pFN21AE1487 5200 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3010 278
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2543 1937
Size: 606 4594
Acc65I G'GTAC_C
Cuts at: 2521 2521
Size: 5200
AclI AA'CG_TT
Cuts at: 1563 3327 3700 1563
Size: 1764 373 3063
Fragments arranged by size:
3063 1764 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2345 3263 4647 4915 1632
Size: 476 237 918 1384 268 1917
Fragments arranged by size:
1917 1384 918 476 268 237
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5003
AflIII A'CryG_T
Cuts at: 1203 4099 5076 1203
Size: 2896 977 1327
Fragments arranged by size:
2896 1327 977
AgeI A'CCGG_T
Cuts at: 4031 4031
Size: 5200
AhdI GACnn_n'nnGTC
Cuts at: 3929 3929
Size: 5200
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2234 1184
Size: 1050 4150
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2351 2383 2351
Size: 32 5168
AlwNI CAG_nnn'CTG
Cuts at: 2137 2197 2486 2943 4515 2137
Size: 60 289 457 1572 2822
Fragments arranged by size:
2822 1572 457 289 60
ApaI G_GGCC'C
Cuts at: 2204 2204
Size: 5200
ApaLI G'TGCA_C
Cuts at: 2117 3257 4413 2117
Size: 1140 1156 2904
Fragments arranged by size:
2904 1156 1140
ApoI r'AATT_y
Cuts at: 1493 2045 2509 2735 2887 4996 1493
Size: 552 464 226 152 2109 1697
Fragments arranged by size:
2109 1697 552 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3754 160
Size: 872 2722 1606
Fragments arranged by size:
2722 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
AvaI C'yCGr_G
Cuts at: 1870 1943 2149 2525 1870
Size: 73 206 376 4545
Fragments arranged by size:
4545 376 206 73
BamHI G'GATC_C
Cuts at: 2530 2897 2530
Size: 367 4833
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 3976 618
Size: 325 217 627 734 1455 1842
Fragments arranged by size:
1842 1455 734 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2204 2519 1391
Size: 813 315 4072
Fragments arranged by size:
4072 813 315
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3391 3425 1903
Size: 34 1454 34 3678
Fragments arranged by size:
3678 1454 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3092 4302 3092
Size: 1210 3990
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5200
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2491 3811 136
Size: 107 122 71 631 605 819 1320 1525
Fragments arranged by size:
1525 1320 819 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5195 5195
Size: 5200
BlpI GC'TnA_GC
Cuts at: 2617 2617
Size: 5200
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2204 3261 4417 1790
Size: 331 83 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 331 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 3889 442
Size: 416 53 83 399 24 1514 958 1753
Fragments arranged by size:
1753 1514 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2417 3860 1239
Size: 1127 51 1443 2579
Fragments arranged by size:
2579 1443 1127 51
Bpu10I CC'TnA_GC
Cuts at: 2060 2438 2060
Size: 378 4822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3325 4190 4488 2414
Size: 911 865 298 3126
Fragments arranged by size:
3126 911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3863 882
Size: 617 2364 2219
Fragments arranged by size:
2364 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2991 493
Size: 711 1787 2702
Fragments arranged by size:
2702 1787 711
BsaBI GATnn'nnATC
Cuts at: 5194 5194
Size: 5200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3007 3389 4024 275
Size: 53 83 186 936 1474 382 635 1451
Fragments arranged by size:
1474 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3633 4031 4305 4452 4863 3633
Size: 398 274 147 411 3970
Fragments arranged by size:
3970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4975
Fragments arranged by size:
4975 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5200
BseYI C'CCAG_C
Cuts at: 2010 2413 4403 4899 2010
Size: 403 1990 496 2311
Fragments arranged by size:
2311 1990 496 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 1163
Size: 573 639 3988
Fragments arranged by size:
3988 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2650 3411 4439 1540
Size: 275 186 649 761 1028 2301
Fragments arranged by size:
2301 1028 761 649 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2444 3261 3346 4417 2121
Size: 323 817 85 1071 2904
Fragments arranged by size:
2904 1071 817 323 85
BsmI GAATG_Cn'
Cuts at: 2711 2804 2711
Size: 93 5107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2204 2444 2519 3261 3346 4417
Size: 399 331 83 240 75 742 85 1071
Cuts at: 4417 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 331 240 85 83
75
BspEI T'CCGG_A
Cuts at: 4863 4863
Size: 5200
BspHI T'CATG_A
Cuts at: 2451 3089 4819 2451
Size: 638 1730 2832
Fragments arranged by size:
2832 1730 638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2555 844
Size: 1711 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 2340 3087 1394
Size: 429 331 186 747 3507
Fragments arranged by size:
3507 747 429 331 186
BsrDI GCAATG_nn'
Cuts at: 65 1248 3695 3869 65
Size: 1183 2447 174 1396
Fragments arranged by size:
2447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3844 4031 1700
Size: 110 2034 187 2869
Fragments arranged by size:
2869 2034 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5200
BssSI C'ACGA_G
Cuts at: 3260 4272 3260
Size: 1012 4188
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5200
BstBI TT'CG_AA
Cuts at: 4004 4004
Size: 5200
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5200
BstYI r'GATC_y
Cuts at: 1972 2530 2897 3282 3299 4751 4837 4849 5195
Size: 558 367 385 17 1452 86 12 346
Cuts at: 5195 1972
Size: 1977
Fragments arranged by size:
1977 1452 558 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2313 513
Size: 552 939 309 3400
Fragments arranged by size:
3400 939 552 309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4461
Fragments arranged by size:
4461 683 56
BtsI GCAGTG_nn'
Cuts at: 2716 3510 3530 2716
Size: 794 20 4386
Fragments arranged by size:
4386 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5165
DraI TTT'AAA
Cuts at: 2504 2504
Size: 5200
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4207 809
Size: 507 2891 1802
Fragments arranged by size:
2891 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2647 3536 5165
Size: 54 1425 50 275 18 817 889 1629
Cuts at: 5165 8
Size: 43
Fragments arranged by size:
1629 1425 889 817 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2647 1537
Size: 275 835 4090
Fragments arranged by size:
4090 835 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3130 1353
Size: 1777 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2509 3787 4161 4307 387
Size: 1064 138 920 1278 374 146 1280
Fragments arranged by size:
1280 1278 1064 920 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2345 2366 2417 3263 3860 4647
Size: 393 476 237 21 51 846 597 787
Cuts at: 4647 4915 1239
Size: 268 1524
Fragments arranged by size:
1524 846 787 597 476 393 268 237
51 21
EcoO109I rG'GnC_Cy
Cuts at: 2014 2176 2194 2201 2014
Size: 162 18 7 5013
Fragments arranged by size:
5013 162 18 7
EcoRI G'AATT_C
Cuts at: 2045 2509 2887 2045
Size: 464 378 4358
Fragments arranged by size:
4358 464 378
FspI TGC'GCA
Cuts at: 3706 3706
Size: 5200
HaeII r_GCGC'y
Cuts at: 1678 2165 4347 1678
Size: 487 2182 2531
Fragments arranged by size:
2531 2182 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3841 3873 3915 3947 1373
Size: 32 135 32 2269 32 42 32 2626
Fragments arranged by size:
2626 2269 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2544 2790 669
Size: 988 281 606 246 3079
Fragments arranged by size:
3079 988 606 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5200
HpaI GTT'AAC
Cuts at: 2790 2790
Size: 5200
KpnI G_GTAC'C
Cuts at: 2525 2525
Size: 5200
MfeI C'AATT_G
Cuts at: 2799 2799
Size: 5200
MluI A'CGCG_T
Cuts at: 5076 5076
Size: 5200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4314 4498 1305
Size: 3009 184 2007
Fragments arranged by size:
3009 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3378
Fragments arranged by size:
3378 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2258 3158 3517 3676 518
Size: 648 909 183 900 359 159 2042
Fragments arranged by size:
2042 909 900 648 359 183 159
MspA1I CmG'CkG
Cuts at: 1606 2413 2616 3293 4441 4686 1606
Size: 807 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 807 677 245 203
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3709
Fragments arranged by size:
3709 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5200
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5200
NotI GC'GGCC_GC
Cuts at: 2647 2647
Size: 5200
NspI r_CATG'y
Cuts at: 1132 1229 2558 4103 1132
Size: 97 1329 1545 2229
Fragments arranged by size:
2229 1545 1329 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5200
PciI A'CATG_T
Cuts at: 4099 4099
Size: 5200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2197 1108
Size: 902 187 4111
Fragments arranged by size:
4111 902 187
PmeI GTTT'AAAC
Cuts at: 2504 2504
Size: 5200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 4782 4814 3269
Size: 32 1481 32 3655
Fragments arranged by size:
3655 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 2176 2014
Size: 162 5038
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5200
PsiI TTA'TAA
Cuts at: 2770 2770
Size: 5200
PspOMI G'GGCC_C
Cuts at: 2200 2200
Size: 5200
PstI C_TGCA'G
Cuts at: 830 2169 2552 830
Size: 1339 383 3478
Fragments arranged by size:
3478 1339 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5200
SalI G'TCGA_C
Cuts at: 1936 2542 1936
Size: 606 4594
SanDI GG'GwC_CC
Cuts at: 2176 2176
Size: 5200
SbfI CC_TGCA'GG
Cuts at: 2552 2552
Size: 5200
ScaI AGT'ACT
Cuts at: 3448 5160 3448
Size: 1712 3488
SfcI C'TryA_G
Cuts at: 826 1045 2165 2548 3683 4364 4555 826
Size: 219 1120 383 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 383 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5200
SmaI CCC'GGG
Cuts at: 1872 2527 1872
Size: 655 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 3304 4205 4467 820
Size: 197 926 486 875 901 262 1553
Fragments arranged by size:
1553 926 901 875 486 262 197
SnaBI TAC'GTA
Cuts at: 493 2991 493
Size: 2498 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5200
SphI G_CATG'C
Cuts at: 1132 2558 1132
Size: 1426 3774
SspI AAT'ATT
Cuts at: 5 52 3124 5
Size: 47 3072 2081
Fragments arranged by size:
3072 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5200
StyI C'CwwG_G
Cuts at: 513 1065 2004 2639 513
Size: 552 939 635 3074
Fragments arranged by size:
3074 939 635 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 3227 3244 3397 3582 4027 325
Size: 1255 537 1110 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1110 537 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3446 5158 96
Size: 274 80 33 51 1443 1469 1712 138
Fragments arranged by size:
1712 1469 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5160 5192 5160
Size: 32 5168
Tth111I GACn'n_nGTC
Cuts at: 1316 2174 1316
Size: 858 4342
XbaI T'CTAG_A
Cuts at: 2536 2536
Size: 5200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2194
Size: 5200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5200
XmaI C'CCGG_G
Cuts at: 1870 2525 1870
Size: 655 4545
XmnI GAAnn'nnTTC
Cuts at: 3329 3329
Size: 5200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3008 276
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI