Restriction map for
pFN21AE1486
(Circular) (Six-base) MAPSORT of: pFN21AE1486.seq Check: 2845 from: 1 to: 7414
pFN21AE1486 7414 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2113 3479 2113
Size: 1366 6048
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2193 5224 278
Size: 53 83 186 936 657 3031 2468
Fragments arranged by size:
3031 2468 936 657 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3819 3996 4416 4692 4757 1937
Size: 1882 177 420 276 65 4594
Fragments arranged by size:
4594 1882 420 276 177 65
Acc65I G'GTAC_C
Cuts at: 4735 4735
Size: 7414
AclI AA'CG_TT
Cuts at: 1563 5541 5914 1563
Size: 3978 373 3063
Fragments arranged by size:
3978 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2500 3341 3528 3616 3668 3923 5477 6861
Size: 868 841 187 88 52 255 1554 1384
Cuts at: 6861 7129 1632
Size: 268 1917
Fragments arranged by size:
1917 1554 1384 868 841 268 255 187
88 52
AfeI AGC'GCT
Cuts at: 4005 4005
Size: 7414
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7217
AflIII A'CryG_T
Cuts at: 1203 6313 7290 1203
Size: 5110 977 1327
Fragments arranged by size:
5110 1327 977
AgeI A'CCGG_T
Cuts at: 4656 6245 4656
Size: 1589 5825
AhdI GACnn_n'nnGTC
Cuts at: 6143 6143
Size: 7414
AleI CACnn'nnGTG
Cuts at: 1166 4277 1166
Size: 3111 4303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3659 1184
Size: 2475 4939
AlwNI CAG_nnn'CTG
Cuts at: 3094 3209 5157 6729 3094
Size: 115 1948 1572 3779
Fragments arranged by size:
3779 1948 1572 115
ApaI G_GGCC'C
Cuts at: 2705 3203 2705
Size: 498 6916
ApaLI G'TGCA_C
Cuts at: 4385 5471 6627 4385
Size: 1086 1156 5172
Fragments arranged by size:
5172 1156 1086
ApoI r'AATT_y
Cuts at: 1493 4723 4949 5101 7210 1493
Size: 3230 226 152 2109 1697
Fragments arranged by size:
3230 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5968 160
Size: 872 4936 1606
Fragments arranged by size:
4936 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7414
AvaI C'yCGr_G
Cuts at: 1870 1943 2326 2667 3577 4739 1870
Size: 73 383 341 910 1162 4545
Fragments arranged by size:
4545 1162 910 383 341 73
BamHI G'GATC_C
Cuts at: 4744 5111 4744
Size: 367 7047
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2524 2857 3844 3917 4167
Size: 325 217 627 737 333 987 73 250
Cuts at: 4167 4735 6190 618
Size: 568 1455 1842
Fragments arranged by size:
1842 1455 987 737 627 568 333 325
250 217 73
BanII G_rGCy'C
Cuts at: 1391 2280 2705 3203 3290 3584 4336 4733 1391
Size: 889 425 498 87 294 752 397 4072
Fragments arranged by size:
4072 889 752 498 425 397 294 87
BbeI G_GCGC'C
Cuts at: 2861 2861
Size: 7414
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2193 3561 3776 4595 928
Size: 971 294 1368 215 819 3747
Fragments arranged by size:
3747 1368 971 819 294 215
BbvCI CC'TCA_GC
Cuts at: 2147 2490 3675 2147
Size: 343 1185 5886
Fragments arranged by size:
5886 1185 343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5605 5639 1903
Size: 34 3668 34 3678
Fragments arranged by size:
3678 3668 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5306 6516 5306
Size: 1210 6204
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7414
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6025 136
Size: 107 122 71 631 605 4353 1525
Fragments arranged by size:
4353 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3924 3963 7409 3924
Size: 39 3446 3929
Fragments arranged by size:
3929 3446 39
BlpI GC'TnA_GC
Cuts at: 4831 4831
Size: 7414
Bme1580I G_kGCm'C
Cuts at: 1790 2447 2705 3203 3630 4170 4389 5475 6631
Size: 657 258 498 427 540 219 1086 1156
Cuts at: 6631 1790
Size: 2573
Fragments arranged by size:
2573 1156 1086 657 540 498 427 258
219
BmgBI CAC'GTC
Cuts at: 1318 4420 1318
Size: 3102 4312
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2214 2864 2924
Size: 416 53 83 399 24 797 650 60
Cuts at: 2924 3170 3408 4271 4678 5145 6103 442
Size: 246 238 863 407 467 958 1753
Fragments arranged by size:
1753 958 863 797 650 467 416 407
399 246 238 83 60 53 24
BmtI G_CTAG'C
Cuts at: 1056 2405 1056
Size: 1349 6065
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 7414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2276 2417 2461 3498 6074 1239
Size: 981 56 141 44 1037 2576 2579
Fragments arranged by size:
2579 2576 1037 981 141 56 44
Bpu10I CC'TnA_GC
Cuts at: 2147 2490 3675 4575 2147
Size: 343 1185 900 4986
Fragments arranged by size:
4986 1185 900 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3749 5539 6404 6702 3749
Size: 1790 865 298 4461
Fragments arranged by size:
4461 1790 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3563 4352 6077 882
Size: 617 2064 789 1725 2219
Fragments arranged by size:
2219 2064 1725 789 617
BsaAI yAC'GTr
Cuts at: 493 1204 3310 4384 5205 493
Size: 711 2106 1074 821 2702
Fragments arranged by size:
2702 2106 1074 821 711
BsaBI GATnn'nnATC
Cuts at: 7408 7408
Size: 7414
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 2858 2880 5221
Size: 53 83 186 936 657 668 22 2341
Cuts at: 5221 5603 6238 275
Size: 382 635 1451
Fragments arranged by size:
2341 1451 936 668 657 635 382 186
83 53 22
BsaWI w'CCGG_w
Cuts at: 4522 4656 5847 6245 6519 6666 7077 4522
Size: 134 1191 398 274 147 411 4859
Fragments arranged by size:
4859 1191 411 398 274 147 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2218 2248 2771 2801 3365
Size: 30 165 30 815 30 523 30 564
Cuts at: 3365 3395 4458 4488 4626 4656 1178
Size: 30 1063 30 138 30 3936
Fragments arranged by size:
3936 1063 815 564 523 165 138 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2939 2960 2981 3002 3023 3044 4370
Size: 865 888 21 21 21 21 21 1326
Cuts at: 4370 1186
Size: 4230
Fragments arranged by size:
4230 1326 888 865 21 21 21 21
21
BseYI C'CCAG_C
Cuts at: 2281 2705 6617 7113 2281
Size: 424 3912 496 2582
Fragments arranged by size:
3912 2582 496 424
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2795 3354 4199 1163
Size: 573 426 633 559 845 4378
Fragments arranged by size:
4378 845 633 573 559 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 4864 5625 6653 1540
Size: 275 186 1864 999 761 1028 2301
Fragments arranged by size:
2301 1864 1028 999 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4389 4425 5475 5560 6631 4389
Size: 36 1050 85 1071 5172
Fragments arranged by size:
5172 1071 1050 85 36
BsmI GAATG_Cn'
Cuts at: 2331 4925 5018 2331
Size: 2594 93 4727
Fragments arranged by size:
4727 2594 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2447 2705 3203 3290 3584 3630
Size: 399 490 167 258 498 87 294 46
Cuts at: 3630 4170 4336 4389 4425 4733 5475 5560 6631
Size: 540 166 53 36 308 742 85 1071
Cuts at: 6631 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 540 498 490 399 308
294 258 167 166 87 85 53 46
36
BspEI T'CCGG_A
Cuts at: 7077 7077
Size: 7414
BspHI T'CATG_A
Cuts at: 5303 7033 5303
Size: 1730 5684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2113 3110 3479 4769 844
Size: 1269 997 369 1290 3489
Fragments arranged by size:
3489 1290 1269 997 369
BsrBI CCG'CTC
Cuts at: 1394 1823 5301 1394
Size: 429 3478 3507
Fragments arranged by size:
3507 3478 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2039 2285 2926 3643 4064 5909 6083
Size: 1183 791 246 641 717 421 1845 174
Cuts at: 6083 65
Size: 1396
Fragments arranged by size:
1845 1396 1183 791 717 641 421 246
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4656 6058 6245 1700
Size: 110 2846 1402 187 2869
Fragments arranged by size:
2869 2846 1402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7414
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7414
BssSI C'ACGA_G
Cuts at: 5474 6486 5474
Size: 1012 6402
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3239 4571 794
Size: 2445 1332 3637
Fragments arranged by size:
3637 2445 1332
BstBI TT'CG_AA
Cuts at: 6218 6218
Size: 7414
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7414
BstXI CCAn_nnnn'nTGG
Cuts at: 4279 4369 4279
Size: 90 7324
BstYI r'GATC_y
Cuts at: 1972 3924 3963 4744 5111 5496 5513 6965 7051
Size: 1952 39 781 367 385 17 1452 86
Cuts at: 7051 7063 7409 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1952 1452 781 385 367 346 86
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 3102 3690 3744 4131 513
Size: 552 939 177 921 588 54 387 3796
Fragments arranged by size:
3796 939 921 588 552 387 177 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6675
Fragments arranged by size:
6675 683 56
BtsI GCAGTG_nn'
Cuts at: 3207 3520 3608 4079 4565 4930 5724 5744 3207
Size: 313 88 471 486 365 794 20 4877
Fragments arranged by size:
4877 794 486 471 365 313 88 20
ClaI AT'CG_AT
Cuts at: 1554 2429 1554
Size: 875 6539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3088 3123 529
Size: 35 2524 35 4820
Fragments arranged by size:
4820 2524 35 35
DraI TTT'AAA
Cuts at: 4718 4718
Size: 7414
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6421 809
Size: 507 5105 1802
Fragments arranged by size:
5105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3096 4861 5750
Size: 54 1425 50 275 18 1266 1765 889
Cuts at: 5750 7379 8
Size: 1629 43
Fragments arranged by size:
1765 1629 1425 1266 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4861 1537
Size: 275 3049 4090
Fragments arranged by size:
4090 3049 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2738 2778 3156 3317 3414 5344 1353
Size: 680 705 40 378 161 97 1930 3423
Fragments arranged by size:
3423 1930 705 680 378 161 97 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4410 6001 6375 6521 387
Size: 1064 138 2821 1591 374 146 1280
Fragments arranged by size:
2821 1591 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2276 2417 2461 2500 3341 3498
Size: 393 588 56 141 44 39 841 157
Cuts at: 3498 3528 3616 3668 3923 5477 6074 6861 7129
Size: 30 88 52 255 1554 597 787 268
Cuts at: 7129 1239
Size: 1524
Fragments arranged by size:
1554 1524 841 787 597 588 393 268
255 157 141 88 56 52 44 39
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2518 2518
Size: 7414
EcoO109I rG'GnC_Cy
Cuts at: 2701 2702 4320 2701
Size: 1 1618 5795
Fragments arranged by size:
5795 1618 1
EcoRI G'AATT_C
Cuts at: 4723 5101 4723
Size: 378 7036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3516 3516
Size: 7414
FspI TGC'GCA
Cuts at: 4214 5920 4214
Size: 1706 5708
HaeII r_GCGC'y
Cuts at: 1678 2060 2861 3889 4007 6561 1678
Size: 382 801 1028 118 2554 2531
Fragments arranged by size:
2554 2531 1028 801 382 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4458 4490 6055 6087 6129
Size: 32 135 32 2886 32 1565 32 42
Cuts at: 6129 6161 1373
Size: 32 2626
Fragments arranged by size:
2886 2626 1565 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3820 4150 4758 5004 669
Size: 988 281 1882 330 608 246 3079
Fragments arranged by size:
3079 1882 988 608 330 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7414
HpaI GTT'AAC
Cuts at: 5004 5004
Size: 7414
KasI G'GCGC_C
Cuts at: 2857 2857
Size: 7414
KpnI G_GTAC'C
Cuts at: 4739 4739
Size: 7414
MfeI C'AATT_G
Cuts at: 5013 5013
Size: 7414
MluI A'CGCG_T
Cuts at: 7290 7290
Size: 7414
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3828 4612 4619 6528 6712 1305
Size: 2523 784 7 1909 184 2007
Fragments arranged by size:
2523 2007 1909 784 184 7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3098 10
Size: 54 1425 343 1266 4326
Fragments arranged by size:
4326 1425 1266 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 3446 3473 4130 4277 5372 5731
Size: 648 1185 1095 27 657 147 1095 359
Cuts at: 5731 5890 518
Size: 159 2042
Fragments arranged by size:
2042 1185 1095 1095 657 648 359 159
147 27
MspA1I CmG'CkG
Cuts at: 1606 2146 2489 3236 4618 4830 5507 6655 6900
Size: 540 343 747 1382 212 677 1148 245
Cuts at: 6900 1606
Size: 2120
Fragments arranged by size:
2120 1382 1148 747 677 540 343 245
212
NarI GG'CG_CC
Cuts at: 2858 2858
Size: 7414
NcoI C'CATG_G
Cuts at: 513 1065 2004 3690 513
Size: 552 939 1686 4237
Fragments arranged by size:
4237 1686 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7414
NheI G'CTAG_C
Cuts at: 1052 2401 1052
Size: 1349 6065
NotI GC'GGCC_GC
Cuts at: 4861 4861
Size: 7414
NspI r_CATG'y
Cuts at: 1132 1229 2678 3478 4772 6317 1132
Size: 97 1449 800 1294 1545 2229
Fragments arranged by size:
2229 1545 1449 1294 800 97
PasI CC'CwG_GG
Cuts at: 1348 3585 1348
Size: 2237 5177
PciI A'CATG_T
Cuts at: 6313 6313
Size: 7414
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2764 1108
Size: 1656 5758
PfoI T'CCnGG_A
Cuts at: 3514 3514
Size: 7414
PmeI GTTT'AAAC
Cuts at: 4718 4718
Size: 7414
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5483 5515 6996 7028 5483
Size: 32 1481 32 5869
Fragments arranged by size:
5869 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2193 1613
Size: 580 6834
PsiI TTA'TAA
Cuts at: 4984 4984
Size: 7414
PspOMI G'GGCC_C
Cuts at: 2701 3199 2701
Size: 498 6916
PspXI vC'TCGA_Gb
Cuts at: 3577 3577
Size: 7414
PstI C_TGCA'G
Cuts at: 830 2275 4766 830
Size: 1445 2491 3478
Fragments arranged by size:
3478 2491 1445
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7414
PvuII CAG'CTG
Cuts at: 2146 2489 3236 4618 2146
Size: 343 747 1382 4942
Fragments arranged by size:
4942 1382 747 343
RsrII CG'GwC_CG
Cuts at: 3430 3430
Size: 7414
SalI G'TCGA_C
Cuts at: 1936 3818 4756 1936
Size: 1882 938 4594
Fragments arranged by size:
4594 1882 938
SapI GCTCTTCn'nnn_
Cuts at: 2033 2738 3317 2033
Size: 705 579 6130
Fragments arranged by size:
6130 705 579
SbfI CC_TGCA'GG
Cuts at: 4766 4766
Size: 7414
ScaI AGT'ACT
Cuts at: 3622 5662 7374 3622
Size: 2040 1712 3662
Fragments arranged by size:
3662 2040 1712
SexAI A'CCwGG_T
Cuts at: 4152 4152
Size: 7414
SfcI C'TryA_G
Cuts at: 826 1045 2271 4762 5897 6578 6769 826
Size: 219 1226 2491 1135 681 191 1471
Fragments arranged by size:
2491 1471 1226 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2859 2859
Size: 7414
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7414
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7414
SmaI CCC'GGG
Cuts at: 1872 4741 1872
Size: 2869 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3577 3764 5518 6419 6681 820
Size: 197 926 1634 187 1754 901 262 1553
Fragments arranged by size:
1754 1634 1553 926 901 262 197 187
SnaBI TAC'GTA
Cuts at: 493 5205 493
Size: 4712 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7414
SphI G_CATG'C
Cuts at: 1132 4772 1132
Size: 3640 3774
SspI AAT'ATT
Cuts at: 5 52 5338 5
Size: 47 5286 2081
Fragments arranged by size:
5286 2081 47
StuI AGG'CCT
Cuts at: 1517 3273 4677 1517
Size: 1756 1404 4254
Fragments arranged by size:
4254 1756 1404
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2528 3148 3345 3690 4853
Size: 552 939 30 494 620 197 345 1163
Cuts at: 4853 513
Size: 3074
Fragments arranged by size:
3074 1163 939 620 552 494 345 197
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2316 3851 3965 5441 5458 5611 5796
Size: 1255 736 1535 114 1476 17 153 185
Cuts at: 5796 6241 325
Size: 445 1498
Fragments arranged by size:
1535 1498 1476 1255 736 445 185 153
114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2387 2467 3620
Size: 274 80 33 51 1443 410 80 1153
Cuts at: 3620 5660 7372 96
Size: 2040 1712 138
Fragments arranged by size:
2040 1712 1443 1153 410 274 138 80
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2218 2250 3365 3397 4626 4658 7374 7406 2218
Size: 32 1115 32 1229 32 2716 32 2226
Fragments arranged by size:
2716 2226 1229 1115 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4445 1316
Size: 3129 4285
XbaI T'CTAG_A
Cuts at: 2437 4750 2437
Size: 2313 5101
XhoI C'TCGA_G
Cuts at: 1943 3577 1943
Size: 1634 5780
XmaI C'CCGG_G
Cuts at: 1870 4739 1870
Size: 2869 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4476 5543 4025
Size: 451 1067 5896
Fragments arranged by size:
5896 1067 451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2191 5222 276
Size: 53 83 186 936 657 3031 2468
Fragments arranged by size:
3031 2468 936 657 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PsrI SacI SacII
SanDI SfiI SrfI SwaI XcmI