Restriction map for
pFN21AE1485
(Circular) (Six-base) MAPSORT of: pFN21AE1485.seq Check: 1215 from: 1 to: 5974
pFN21AE1485 5974 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2235 3062 3317 1937
Size: 298 827 255 4594
Fragments arranged by size:
4594 827 298 255
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4037 5421 5689 1632
Size: 2405 1384 268 1917
Fragments arranged by size:
2405 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 2796 1166
Size: 1630 4344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 4858
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 5942
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2268 2405 3283 3509 3661 5770 1493
Size: 775 137 878 226 152 2109 1697
Fragments arranged by size:
2109 1697 878 775 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 3299 1870
Size: 73 400 956 4545
Fragments arranged by size:
4545 956 400 73
BamHI G'GATC_C
Cuts at: 2453 3304 3671 2453
Size: 851 367 4756
Fragments arranged by size:
4756 851 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3293 1391
Size: 1902 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2056 928
Size: 971 157 4846
Fragments arranged by size:
4846 971 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BfrBI ATG'CAT
Cuts at: 2225 2932 2225
Size: 707 5267
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2302 5969 2302
Size: 3667 2307
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 3176 4035 5191 1790
Size: 1386 859 1156 2573
Fragments arranged by size:
2573 1386 1156 859
BmgBI CAC'GTC
Cuts at: 1318 2296 1318
Size: 978 4996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3123 4634 1239
Size: 1884 1511 2579
Fragments arranged by size:
2579 1884 1511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2911 4099 4964 5262 2911
Size: 1188 865 298 3623
Fragments arranged by size:
3623 1188 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5974
BseYI C'CCAG_C
Cuts at: 2943 5177 5673 2943
Size: 2234 496 3244
Fragments arranged by size:
3244 2234 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4035 4120 5191 4035
Size: 85 1071 4818
Fragments arranged by size:
4818 1071 85
BsmI GAATG_Cn'
Cuts at: 2932 3485 3578 2932
Size: 553 93 5328
Fragments arranged by size:
5328 553 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3176 3293 4035 4120 5191 1391
Size: 399 1386 117 742 85 1071 2174
Fragments arranged by size:
2174 1386 1071 742 399 117 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2265 3861 1394
Size: 429 442 1596 3507
Fragments arranged by size:
3507 1596 442 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2749 4469 4643 65
Size: 1183 1501 1720 174 1396
Fragments arranged by size:
1720 1501 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2170 2665 4618 4805 1700
Size: 110 360 495 1953 187 2869
Fragments arranged by size:
2869 1953 495 360 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3029 794
Size: 2235 3739
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2302 2453 2559 3120 3304 3671 4056 4073
Size: 330 151 106 561 184 367 385 17
Cuts at: 4073 5525 5611 5623 5969 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 561 385 367 346 330 184
151 106 86 17 12
BstZ17I GTA'TAC
Cuts at: 3063 3063
Size: 5974
Bsu36I CC'TnA_GG
Cuts at: 2639 2639
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 3073 513
Size: 552 2008 3414
Fragments arranged by size:
3414 2008 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2284 2883 3490 4284 4304 2284
Size: 599 607 794 20 3954
Fragments arranged by size:
3954 794 607 599 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 2256 2487 3278 2256
Size: 231 791 4952
Fragments arranged by size:
4952 791 231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 3070 3421
Size: 54 1425 50 275 18 250 990 351
Cuts at: 3421 4310 5939 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 990 889 351 275 250 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2103 3904 1353
Size: 698 52 1801 3423
Fragments arranged by size:
3423 1801 698 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3123 4037 4634 5421 5689 1239
Size: 393 1491 914 597 787 268 1524
Fragments arranged by size:
1524 1491 914 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2268 3283 3661 2268
Size: 1015 378 4581
Fragments arranged by size:
4581 1015 378
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2544 2576 2987 3019 4615
Size: 32 135 32 972 32 411 32 1596
Cuts at: 4615 4647 4689 4721 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1596 972 411 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 3318 3564 669
Size: 988 281 298 1082 246 3079
Fragments arranged by size:
3079 1082 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 2009 3225 748
Size: 1261 1216 3497
Fragments arranged by size:
3497 1261 1216
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3153 3573 3153
Size: 420 5554
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 5088 5272 1305
Size: 1044 2739 184 2007
Fragments arranged by size:
2739 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2082 3072 10
Size: 54 1425 343 250 990 2912
Fragments arranged by size:
2912 1425 990 343 250 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 2796 2927 3072 3932 4291 4450
Size: 648 1090 540 131 145 860 359 159
Cuts at: 4450 518
Size: 2042
Fragments arranged by size:
2042 1090 860 648 540 359 159 145
131
MspA1I CmG'CkG
Cuts at: 1606 2180 3236 3390 4067 5215 5460 1606
Size: 574 1056 154 677 1148 245 2120
Fragments arranged by size:
2120 1148 1056 677 574 245 154
NcoI C'CATG_G
Cuts at: 513 1065 3073 513
Size: 552 2008 3414
Fragments arranged by size:
3414 2008 552
NdeI CA'TA_TG
Cuts at: 387 2149 2525 387
Size: 1762 376 3836
Fragments arranged by size:
3836 1762 376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NsiI A_TGCA'T
Cuts at: 2227 2934 2227
Size: 707 5267
NspI r_CATG'y
Cuts at: 1132 1229 2063 2932 3260 3332 4877 1132
Size: 97 834 869 328 72 1545 2229
Fragments arranged by size:
2229 1545 869 834 328 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2556 2793 1108
Size: 1448 237 4289
Fragments arranged by size:
4289 1448 237
PfoI T'CCnGG_A
Cuts at: 3100 3100
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 2411 3544 2411
Size: 1133 4841
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2025 2057 2025
Size: 32 5942
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2180 2180
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 2234 3316 1936
Size: 298 1082 4594
Fragments arranged by size:
4594 1082 298
SapI GCTCTTCn'nnn_
Cuts at: 2103 2103
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 3056 3056
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 2129 3217 3322 4457 5138 5329 826
Size: 219 1084 1088 105 1135 681 191 1471
Fragments arranged by size:
1471 1135 1088 1084 681 219 191 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2890 4078 4979 5241 820
Size: 197 926 947 1188 901 262 1553
Fragments arranged by size:
1553 1188 947 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 3044 152
Size: 2892 3082
SphI G_CATG'C
Cuts at: 1132 2932 3332 1132
Size: 1800 400 3774
Fragments arranged by size:
3774 1800 400
SspI AAT'ATT
Cuts at: 5 52 3143 3898 5
Size: 47 3091 755 2081
Fragments arranged by size:
3091 2081 755 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 3073 3413 513
Size: 552 2008 340 3074
Fragments arranged by size:
3074 2008 552 340
SwaI ATTT'AAAT
Cuts at: 2256 2256
Size: 5974
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4001 4018 4171 4356 4801 325
Size: 1255 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 2521 2553 5934 5966 2438
Size: 32 51 32 3381 32 2446
Fragments arranged by size:
3381 2446 51 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2333 1316
Size: 1017 4957
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 3024 4103 3024
Size: 1079 4895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SfiI SfoI SrfI XcmI