Restriction map for
pFN21AE1484
(Circular) (Six-base) MAPSORT of: pFN21AE1484.seq Check: 8465 from: 1 to: 6670
pFN21AE1484 6670 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3965 4480 278
Size: 53 83 186 936 2429 515 2468
Fragments arranged by size:
2468 2429 936 515 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 4013 1937
Size: 822 1254 4594
Fragments arranged by size:
4594 1254 822
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2271 2825 4733 6117 6385 1632
Size: 483 156 554 1908 1384 268 1917
Fragments arranged by size:
1917 1908 1384 554 483 268 156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 3091 5569 6546 1203
Size: 1888 2478 977 1327
Fragments arranged by size:
2478 1888 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 3092 1166
Size: 1926 4744
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2645 1184
Size: 1461 5209
AlwNI CAG_nnn'CTG
Cuts at: 2474 3511 4413 5985 2474
Size: 1037 902 1572 3159
Fragments arranged by size:
3159 1572 1037 902
ApaLI G'TGCA_C
Cuts at: 2972 4727 5883 2972
Size: 1755 1156 3759
Fragments arranged by size:
3759 1755 1156
ApoI r'AATT_y
Cuts at: 1493 2225 2577 2833 2909 3306 3573 3979 4205
Size: 732 352 256 76 397 267 406 226
Cuts at: 4205 4357 6466 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 732 406 397 352 267 256
226 152 76
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 3995 1870
Size: 73 2052 4545
Fragments arranged by size:
4545 2052 73
BamHI G'GATC_C
Cuts at: 4000 4367 4000
Size: 367 6303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2536 3851 3991 5446 618
Size: 325 217 627 749 1315 140 1455 1842
Fragments arranged by size:
1842 1455 1315 749 627 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2145 3492 3989 1391
Size: 754 1347 497 4072
Fragments arranged by size:
4072 1347 754 497
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2778 3681 928
Size: 971 879 903 3917
Fragments arranged by size:
3917 971 903 879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3918 3952 4861 4895 1903
Size: 34 1981 34 909 34 3678
Fragments arranged by size:
3678 1981 909 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3841 5281 136
Size: 107 122 71 631 605 2169 1440 1525
Fragments arranged by size:
2169 1525 1440 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3247 6665 3247
Size: 3418 3252
BlpI GC'TnA_GC
Cuts at: 3041 4087 3041
Size: 1046 5624
Bme1580I G_kGCm'C
Cuts at: 1790 2976 4731 5887 1790
Size: 1186 1755 1156 2573
Fragments arranged by size:
2573 1755 1186 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6670
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2034 4401 5359
Size: 416 53 83 399 24 617 2367 958
Cuts at: 5359 442
Size: 1753
Fragments arranged by size:
2367 1753 958 617 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3634 3637 3634
Size: 3 6667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2304 3770 5330 1239
Size: 1065 1466 1560 2579
Fragments arranged by size:
2579 1560 1466 1065
Bpu10I CC'TnA_GC
Cuts at: 3456 3456
Size: 6670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3902 4795 5660 5958 3902
Size: 893 865 298 4614
Fragments arranged by size:
4614 893 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2814 3176 5333 882
Size: 617 1315 362 2157 2219
Fragments arranged by size:
2219 2157 1315 617 362
BsaAI yAC'GTr
Cuts at: 493 1204 2662 4461 493
Size: 711 1458 1799 2702
Fragments arranged by size:
2702 1799 1458 711
BsaBI GATnn'nnATC
Cuts at: 6664 6664
Size: 6670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3876 3962 4477 4859
Size: 53 83 186 936 2343 86 515 382
Cuts at: 4859 5494 275
Size: 635 1451
Fragments arranged by size:
2343 1451 936 635 515 382 186 86
83 53
BsaWI w'CCGG_w
Cuts at: 5103 5501 5775 5922 6333 5103
Size: 398 274 147 411 5440
Fragments arranged by size:
5440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3824 3854 1178
Size: 30 165 30 2421 30 3994
Fragments arranged by size:
3994 2421 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2768 3218 3500 3610 3626 3665 3668 3755
Size: 1582 450 282 110 16 39 3 87
Cuts at: 3755 3845 1186
Size: 90 4011
Fragments arranged by size:
4011 1582 450 282 110 90 87 39
16 3
BseYI C'CCAG_C
Cuts at: 2087 2348 3941 5873 6369 2087
Size: 261 1593 1932 496 2388
Fragments arranged by size:
2388 1932 1593 496 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2924 3074 3791 1163
Size: 573 1188 150 717 4042
Fragments arranged by size:
4042 1188 717 573 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4120 4881 5909 1540
Size: 275 186 1597 522 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 522 275 186
BsiHKAI G_wGCw'C
Cuts at: 2976 3042 3492 4731 4816 5887 2976
Size: 66 450 1239 85 1071 3759
Fragments arranged by size:
3759 1239 1071 450 85 66
BsmI GAATG_Cn'
Cuts at: 2622 4181 4274 2622
Size: 1559 93 5018
Fragments arranged by size:
5018 1559 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3864 3953 3864
Size: 89 6581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2976 3042 3492 3989 4731 4816
Size: 399 355 831 66 450 497 742 85
Cuts at: 4816 5887 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 831 742 497 450 399 355
85 66
BspEI T'CCGG_A
Cuts at: 6333 6333
Size: 6670
BspHI T'CATG_A
Cuts at: 3736 4559 6289 3736
Size: 823 1730 4117
Fragments arranged by size:
4117 1730 823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4025 844
Size: 3181 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4557 1394
Size: 429 2734 3507
Fragments arranged by size:
3507 2734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2988 5165 5339 65
Size: 1183 1740 2177 174 1396
Fragments arranged by size:
2177 1740 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3516 3925 5314 5501 1700
Size: 110 1706 409 1389 187 2869
Fragments arranged by size:
2869 1706 1389 409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 4730 5742 4730
Size: 1012 5658
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 3511 794
Size: 2021 696 3953
Fragments arranged by size:
3953 2021 696
BstBI TT'CG_AA
Cuts at: 5474 5474
Size: 6670
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6670
BstYI r'GATC_y
Cuts at: 1972 3247 3468 4000 4367 4752 4769 6221 6307
Size: 1275 221 532 367 385 17 1452 86
Cuts at: 6307 6319 6665 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1275 532 385 367 346 221
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2886 3498 513
Size: 552 1821 612 3685
Fragments arranged by size:
3685 1821 612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3160 3921 487
Size: 683 56 1934 761 3236
Fragments arranged by size:
3236 1934 761 683 56
BtsI GCAGTG_nn'
Cuts at: 4186 4980 5000 4186
Size: 794 20 5856
Fragments arranged by size:
5856 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 2380 3974 2380
Size: 1594 5076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5677 809
Size: 507 4361 1802
Fragments arranged by size:
4361 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3159 3495 3595
Size: 54 1425 50 275 18 1329 336 100
Cuts at: 3595 4117 5006 6635 8
Size: 522 889 1629 43
Fragments arranged by size:
1629 1425 1329 889 522 336 275 100
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 4117 1537
Size: 275 1783 522 4090
Fragments arranged by size:
4090 1783 522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2309 2862 3408 3534 3690 4600 1353
Size: 738 218 553 546 126 156 910 3423
Fragments arranged by size:
3423 910 738 553 546 218 156 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5257 5631 5777 387
Size: 1064 138 3668 374 146 1280
Fragments arranged by size:
3668 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3490 3490
Size: 6670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2271 2304 2825 3770 4733 5330
Size: 393 483 156 33 521 945 963 597
Cuts at: 5330 6117 6385 1239
Size: 787 268 1524
Fragments arranged by size:
1524 963 945 787 597 521 483 393
268 156 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3455 3455
Size: 6670
EcoRI G'AATT_C
Cuts at: 2577 2909 3979 4357 2577
Size: 332 1070 378 4890
Fragments arranged by size:
4890 1070 378 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 5817 1678
Size: 4139 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2438 2470 3049
Size: 32 135 32 599 32 235 32 579
Cuts at: 3049 3081 3124 3156 3370 3402 3861 3893 5311
Size: 32 43 32 214 32 459 32 1418
Cuts at: 5311 5343 5385 5417 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1418 599 579 459 235 214 135
43 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4014 4260 669
Size: 988 281 2076 246 3079
Fragments arranged by size:
3079 2076 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6670
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5784 5968 1305
Size: 4479 184 2007
Fragments arranged by size:
4479 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3161 3497 10
Size: 54 1425 343 1329 336 3183
Fragments arranged by size:
3183 1425 1329 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 4628 4987 5146 518
Size: 648 1926 1536 359 159 2042
Fragments arranged by size:
2042 1926 1536 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2474 3775 4086 4763 5911 6156 1606
Size: 464 404 1301 311 677 1148 245 2120
Fragments arranged by size:
2120 1301 1148 677 464 404 311 245
NaeI GCC'GGC
Cuts at: 3518 3927 3518
Size: 409 6261
NcoI C'CATG_G
Cuts at: 513 1065 3498 513
Size: 552 2433 3685
Fragments arranged by size:
3685 2433 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NgoMIV G'CCGG_C
Cuts at: 3516 3925 3516
Size: 409 6261
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 3095 4028 5573 1132
Size: 97 1866 933 1545 2229
Fragments arranged by size:
2229 1866 1545 933 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6670
PciI A'CATG_T
Cuts at: 3091 5569 3091
Size: 2478 4192
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6670
PfoI T'CCnGG_A
Cuts at: 2119 2119
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 3440 4240 3440
Size: 800 5870
PstI C_TGCA'G
Cuts at: 830 3255 3663 4022 830
Size: 2425 408 359 3478
Fragments arranged by size:
3478 2425 408 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
PvuII CAG'CTG
Cuts at: 2474 3775 2474
Size: 1301 5369
SacI G_AGCT'C
Cuts at: 3492 3492
Size: 6670
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SapI GCTCTTCn'nnn_
Cuts at: 3690 3690
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 3617 4918 6630 3617
Size: 1301 1712 3657
Fragments arranged by size:
3657 1712 1301
SfcI C'TryA_G
Cuts at: 826 1045 2695 2790 3033 3251 3659 4018 5153
Size: 219 1650 95 243 218 408 359 1135
Cuts at: 5153 5834 6025 826
Size: 681 191 1471
Fragments arranged by size:
1650 1471 1135 681 408 359 243 219
218 191 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 3997 1872
Size: 2125 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3881 4774 5675 5937 820
Size: 197 926 1938 893 901 262 1553
Fragments arranged by size:
1938 1553 926 901 893 262 197
SnaBI TAC'GTA
Cuts at: 493 2662 4461 493
Size: 2169 1799 2702
Fragments arranged by size:
2702 2169 1799
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 4028 1132
Size: 2896 3774
SspI AAT'ATT
Cuts at: 5 52 3445 4594 5
Size: 47 3393 1149 2081
Fragments arranged by size:
3393 2081 1149 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6670
StyI C'CwwG_G
Cuts at: 513 1065 2322 3498 3801 4109 513
Size: 552 1257 1176 303 308 3074
Fragments arranged by size:
3074 1257 1176 552 308 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4697 4714 4867 5052 5497 325
Size: 1255 3117 17 153 185 445 1498
Fragments arranged by size:
3117 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3298 3615 4916
Size: 274 80 33 51 1443 1321 317 1301
Cuts at: 4916 6628 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1321 1301 317 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3824 3856 6630 6662 3824
Size: 32 2774 32 3832
Fragments arranged by size:
3832 2774 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2984 3875 1316
Size: 1668 891 4111
Fragments arranged by size:
4111 1668 891
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 3995 1870
Size: 2125 4545
XmnI GAAnn'nnTTC
Cuts at: 4799 4799
Size: 6670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3963 4478 276
Size: 53 83 186 936 2429 515 2468
Fragments arranged by size:
2468 2429 936 515 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI