Restriction map for
pFN21AE1482
(Circular) (Six-base) MAPSORT of: pFN21AE1482.seq Check: 2778 from: 1 to: 7195
pFN21AE1482 7195 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2084 2507 2872 3975 2084
Size: 423 365 1103 5304
Fragments arranged by size:
5304 1103 423 365
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5005 278
Size: 53 83 186 936 3469 2468
Fragments arranged by size:
3469 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4538 1937
Size: 2601 4594
Acc65I G'GTAC_C
Cuts at: 4516 4516
Size: 7195
AclI AA'CG_TT
Cuts at: 1563 5322 5695 1563
Size: 3759 373 3063
Fragments arranged by size:
3759 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2581 3310 3382 5258 6642 6910 1632
Size: 949 729 72 1876 1384 268 1917
Fragments arranged by size:
1917 1876 1384 949 729 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6998
AflIII A'CryG_T
Cuts at: 1203 3840 6094 7071 1203
Size: 2637 2254 977 1327
Fragments arranged by size:
2637 2254 1327 977
AgeI A'CCGG_T
Cuts at: 3518 6026 3518
Size: 2508 4687
AhdI GACnn_n'nnGTC
Cuts at: 5924 5924
Size: 7195
AleI CACnn'nnGTG
Cuts at: 1166 2078 3911 1166
Size: 912 1833 4450
Fragments arranged by size:
4450 1833 912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3890 3977 1184
Size: 2706 87 4402
Fragments arranged by size:
4402 2706 87
AlwNI CAG_nnn'CTG
Cuts at: 2066 2326 2356 2497 2617 2782 2803 2869 4938
Size: 260 30 141 120 165 21 66 2069
Cuts at: 4938 6510 2066
Size: 1572 2751
Fragments arranged by size:
2751 2069 1572 260 165 141 120 66
30 21
ApaI G_GGCC'C
Cuts at: 2365 2773 4222 2365
Size: 408 1449 5338
Fragments arranged by size:
5338 1449 408
ApaLI G'TGCA_C
Cuts at: 3635 3677 5252 6408 3635
Size: 42 1575 1156 4422
Fragments arranged by size:
4422 1575 1156 42
ApoI r'AATT_y
Cuts at: 1493 4424 4504 4730 4882 6991 1493
Size: 2931 80 226 152 2109 1697
Fragments arranged by size:
2931 2109 1697 226 152 80
AseI AT'TA_AT
Cuts at: 160 1032 5749 160
Size: 872 4717 1606
Fragments arranged by size:
4717 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7195
AvaI C'yCGr_G
Cuts at: 1870 1943 2277 2477 2765 2816 3424 4520 1870
Size: 73 334 200 288 51 608 1096 4545
Fragments arranged by size:
4545 1096 608 334 288 200 73 51
BamHI G'GATC_C
Cuts at: 3505 4525 4892 3505
Size: 1020 367 5808
Fragments arranged by size:
5808 1020 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 2519 3693 3934 4516
Size: 325 217 627 479 253 1174 241 582
Cuts at: 4516 5971 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1174 627 582 479 325 253
241 217
BanII G_rGCy'C
Cuts at: 1391 2012 2365 2532 2773 3726 4076 4125 4222
Size: 621 353 167 241 953 350 49 97
Cuts at: 4222 4514 1391
Size: 292 4072
Fragments arranged by size:
4072 953 621 353 350 292 241 167
97 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2198 2725 3517 3658 928
Size: 971 299 527 792 141 4465
Fragments arranged by size:
4465 971 792 527 299 141
BbvCI CC'TCA_GC
Cuts at: 2418 2418
Size: 7195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5386 5420 1903
Size: 34 3449 34 3678
Fragments arranged by size:
3678 3449 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5087 6297 5087
Size: 1210 5985
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7195
BfrBI ATG'CAT
Cuts at: 3473 3473
Size: 7195
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 2878 3446
Size: 107 122 71 631 605 688 518 568
Cuts at: 3446 3809 5806 136
Size: 363 1997 1525
Fragments arranged by size:
1997 1525 688 631 605 568 518 363
122 107 71
BglII A'GATC_T
Cuts at: 3309 7190 3309
Size: 3881 3314
BlpI GC'TnA_GC
Cuts at: 4612 4612
Size: 7195
Bme1580I G_kGCm'C
Cuts at: 1790 2365 2773 3639 3681 3939 4222 5256 6412
Size: 575 408 866 42 258 283 1034 1156
Cuts at: 6412 1790
Size: 2573
Fragments arranged by size:
2573 1156 1034 866 575 408 283 258
42
BmgBI CAC'GTC
Cuts at: 1318 2731 1318
Size: 1413 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2683 4426 4926
Size: 416 53 83 399 24 1266 1743 500
Cuts at: 4926 5884 442
Size: 958 1753
Fragments arranged by size:
1753 1743 1266 958 500 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4402 4402
Size: 7195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2453 2584 2627 2639 3451 3484 3728
Size: 968 246 131 43 12 812 33 244
Cuts at: 3728 4090 5855 1239
Size: 362 1765 2579
Fragments arranged by size:
2579 1765 968 812 362 246 244 131
43 33 12
Bpu10I CC'TnA_GC
Cuts at: 2418 2837 4223 2418
Size: 419 1386 5390
Fragments arranged by size:
5390 1386 419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 5320 6185 6483 3604
Size: 1716 865 298 4316
Fragments arranged by size:
4316 1716 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 2979 5858 882
Size: 617 613 867 2879 2219
Fragments arranged by size:
2879 2219 867 617 613
BsaAI yAC'GTr
Cuts at: 493 1204 2649 3676 4986 493
Size: 711 1445 1027 1310 2702
Fragments arranged by size:
2702 1445 1310 1027 711
BsaBI GATnn'nnATC
Cuts at: 7189 7189
Size: 7195
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2459 3630 5002 5384
Size: 53 83 186 936 926 1171 1372 382
Cuts at: 5384 6019 275
Size: 635 1451
Fragments arranged by size:
1451 1372 1171 936 926 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2019 3518 5628 6026 6300 6447 6858 2019
Size: 1499 2110 398 274 147 411 2356
Fragments arranged by size:
2356 2110 1499 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4184 4214 1178
Size: 30 165 30 2781 30 4159
Fragments arranged by size:
4159 2781 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2195 2228 2315 2768 3044 4012 4127
Size: 856 153 33 87 453 276 968 115
Cuts at: 4127 1186
Size: 4254
Fragments arranged by size:
4254 968 856 453 276 153 115 87
33
BseYI C'CCAG_C
Cuts at: 2089 2098 2228 2364 3483 3736 3815 3863 4239
Size: 9 130 136 1119 253 79 48 376
Cuts at: 4239 6398 6894 2089
Size: 2159 496 2390
Fragments arranged by size:
2390 2159 1119 496 376 253 136 130
79 48 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2906 2950 3947 3956 4382 4397
Size: 573 618 552 44 997 9 426 15
Cuts at: 4397 1163
Size: 3961
Fragments arranged by size:
3961 997 618 573 552 426 44 15
9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3284 4645 5406 6434 1540
Size: 275 186 1283 1361 761 1028 2301
Fragments arranged by size:
2301 1361 1283 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2625 2942 2978 3639 3681 3726 4076 4125 4143
Size: 317 36 661 42 45 350 49 18
Cuts at: 4143 5256 5341 6412 2625
Size: 1113 85 1071 3408
Fragments arranged by size:
3408 1113 1071 661 350 317 85 49
45 42 36 18
BsmI GAATG_Cn'
Cuts at: 3473 4706 4799 3473
Size: 1233 93 5869
Fragments arranged by size:
5869 1233 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2143 2297 3704 4192 2143
Size: 154 1407 488 5146
Fragments arranged by size:
5146 1407 488 154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2365 2532 2625 2773 2942 2978
Size: 399 222 353 167 93 148 169 36
Cuts at: 2978 3639 3681 3726 3939 4076 4125 4143 4222
Size: 661 42 45 213 137 49 18 79
Cuts at: 4222 4514 5256 5341 6412 1391
Size: 292 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 399 353 292 222
213 169 167 148 137 93 85 79
49 45 42 36 18
BspEI T'CCGG_A
Cuts at: 2019 6858 2019
Size: 4839 2356
BspHI T'CATG_A
Cuts at: 5084 6814 5084
Size: 1730 5465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2084 2292 2507 2872 2878 2997 3348 3975
Size: 1240 208 215 365 6 119 351 627
Cuts at: 3975 4053 4215 4550 844
Size: 78 162 335 3489
Fragments arranged by size:
3489 1240 627 365 351 335 215 208
162 119 78 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2830 3170 5082 1394
Size: 429 1007 340 1912 3507
Fragments arranged by size:
3507 1912 1007 429 340
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 2958 5690 5864 65
Size: 1183 1338 372 2732 174 1396
Fragments arranged by size:
2732 1396 1338 1183 372 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2263 3283 3374 3518 3696 5839 6026
Size: 110 453 1020 91 144 178 2143 187
Cuts at: 6026 1700
Size: 2869
Fragments arranged by size:
2869 2143 1020 453 187 178 144 110
91
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7195
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7195
BssSI C'ACGA_G
Cuts at: 2105 3755 5255 6267 2105
Size: 1650 1500 1012 3033
Fragments arranged by size:
3033 1650 1500 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2356 2377 2782 2869 3063 3493 3700 3970
Size: 1562 21 405 87 194 430 207 270
Cuts at: 3970 4405 794
Size: 435 3584
Fragments arranged by size:
3584 1562 435 430 405 270 207 194
87 21
BstBI TT'CG_AA
Cuts at: 5999 5999
Size: 7195
BstEII G'GTnAC_C
Cuts at: 1184 3749 1184
Size: 2565 4630
BstXI CCAn_nnnn'nTGG
Cuts at: 2441 2649 3913 2441
Size: 208 1264 5723
Fragments arranged by size:
5723 1264 208
BstYI r'GATC_y
Cuts at: 1972 2697 3309 3505 4525 4892 5277 5294 6746
Size: 725 612 196 1020 367 385 17 1452
Cuts at: 6746 6832 6844 7190 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1020 725 612 385 367 346
196 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2498 2498
Size: 7195
BtgI C'CryG_G
Cuts at: 513 1065 2004 2523 2575 3045 3229 3261 3444
Size: 552 939 519 52 470 184 32 183
Cuts at: 3444 3930 4263 4356 513
Size: 486 333 93 3352
Fragments arranged by size:
3352 939 552 519 486 470 333 184
183 93 52 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6456
Fragments arranged by size:
6456 683 56
BtsI GCAGTG_nn'
Cuts at: 2722 4711 5505 5525 2722
Size: 1989 794 20 4392
Fragments arranged by size:
4392 1989 794 20
ClaI AT'CG_AT
Cuts at: 1554 3552 1554
Size: 1998 5197
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7160
DraI TTT'AAA
Cuts at: 4499 4499
Size: 7195
DraIII CAC_nnn'GTG
Cuts at: 2629 4420 2629
Size: 1791 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2501 6202 809
Size: 507 1185 3701 1802
Fragments arranged by size:
3701 1802 1185 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3108 3258 3438
Size: 54 1425 50 275 18 1278 150 180
Cuts at: 3438 3447 3685 3927 4642 5531 7160 8
Size: 9 238 242 715 889 1629 43
Fragments arranged by size:
1629 1425 1278 889 715 275 242 238
180 150 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4642 1537
Size: 275 2830 4090
Fragments arranged by size:
4090 2830 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3254 3749 3854 4104 5125 1353
Size: 1901 495 105 250 1021 3423
Fragments arranged by size:
3423 1901 1021 495 250 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5782 6156 6302 387
Size: 1064 138 4193 374 146 1280
Fragments arranged by size:
4193 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3724 4074 4123 3724
Size: 350 49 6796
Fragments arranged by size:
6796 350 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2453 2581 2584 2627 2639 3310
Size: 393 575 246 128 3 43 12 671
Cuts at: 3310 3382 3451 3484 3728 4090 5258 5855 6642
Size: 72 69 33 244 362 1168 597 787
Cuts at: 6642 6910 1239
Size: 268 1524
Fragments arranged by size:
1524 1168 787 671 597 575 393 362
268 246 244 128 72 69 43 33
12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2318 2452 2468 2840 3002 2210
Size: 108 134 16 372 162 6403
Fragments arranged by size:
6403 372 162 134 108 16
EcoO109I rG'GnC_Cy
Cuts at: 2281 2353 2445 2473 2494 2539 2568 2769 2770
Size: 72 92 28 21 45 29 201 1
Cuts at: 2770 2812 3007 3533 4096 4219 2281
Size: 42 195 526 563 123 5257
Fragments arranged by size:
5257 563 526 201 195 123 92 72
45 42 29 28 21 1
EcoRI G'AATT_C
Cuts at: 4424 4504 4882 4424
Size: 80 378 6737
Fragments arranged by size:
6737 378 80
FspI TGC'GCA
Cuts at: 2291 4274 5701 2291
Size: 1983 1427 3785
Fragments arranged by size:
3785 1983 1427
FspAI rTGC'GCAy
Cuts at: 4274 4274
Size: 7195
HaeII r_GCGC'y
Cuts at: 1678 2142 2176 2314 2557 6342 1678
Size: 464 34 138 243 3785 2531
Fragments arranged by size:
3785 2531 464 243 138 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2432 2464 2682 2714 5836
Size: 32 135 32 860 32 218 32 3122
Cuts at: 5836 5868 5910 5942 1373
Size: 32 42 32 2626
Fragments arranged by size:
3122 2626 860 218 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3163 4539 4785 669
Size: 988 281 568 657 1376 246 3079
Fragments arranged by size:
3079 1376 988 657 568 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7195
HpaI GTT'AAC
Cuts at: 4785 4785
Size: 7195
KpnI G_GTAC'C
Cuts at: 4520 4520
Size: 7195
MfeI C'AATT_G
Cuts at: 4794 4794
Size: 7195
MluI A'CGCG_T
Cuts at: 7071 7071
Size: 7195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4215 6309 6493 1305
Size: 2910 2094 184 2007
Fragments arranged by size:
2910 2094 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3260 3449 3687 3929 10
Size: 54 1425 343 1428 189 238 242 3276
Fragments arranged by size:
3276 1428 1425 343 242 238 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2439 2651 3839 3911 4268 5153
Size: 648 912 361 212 1188 72 357 885
Cuts at: 5153 5512 5671 518
Size: 359 159 2042
Fragments arranged by size:
2042 1188 912 885 648 361 359 357
212 159 72
MspA1I CmG'CkG
Cuts at: 1606 2063 2368 2377 2422 2577 2617 2782 2866
Size: 457 305 9 45 155 40 165 84
Cuts at: 2866 4007 4243 4611 5288 6436 6681 1606
Size: 1141 236 368 677 1148 245 2120
Fragments arranged by size:
2120 1148 1141 677 457 368 305 245
236 165 155 84 45 40 9
NaeI GCC'GGC
Cuts at: 2265 2265
Size: 7195
NcoI C'CATG_G
Cuts at: 513 1065 2004 3261 3444 3930 4263 513
Size: 552 939 1257 183 486 333 3445
Fragments arranged by size:
3445 1257 939 552 486 333 183
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7195
NgoMIV G'CCGG_C
Cuts at: 2263 2263
Size: 7195
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7195
NotI GC'GGCC_GC
Cuts at: 4642 4642
Size: 7195
NsiI A_TGCA'T
Cuts at: 3475 3475
Size: 7195
NspI r_CATG'y
Cuts at: 1132 1229 3076 3844 4553 6098 1132
Size: 97 1847 768 709 1545 2229
Fragments arranged by size:
2229 1847 1545 768 709 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7195
PciI A'CATG_T
Cuts at: 3840 6094 3840
Size: 2254 4941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7195
PfoI T'CCnGG_A
Cuts at: 2295 2295
Size: 7195
PmeI GTTT'AAAC
Cuts at: 4499 4499
Size: 7195
PmlI CAC'GTG
Cuts at: 2649 3676 2649
Size: 1027 6168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5264 5296 6777 6809 5264
Size: 32 1481 32 5650
Fragments arranged by size:
5650 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2494 2812 3533 2281
Size: 213 318 721 5943
Fragments arranged by size:
5943 721 318 213
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7195
PsiI TTA'TAA
Cuts at: 4765 4765
Size: 7195
PspOMI G'GGCC_C
Cuts at: 2361 2769 4218 2361
Size: 408 1449 5338
Fragments arranged by size:
5338 1449 408
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 7163
PstI C_TGCA'G
Cuts at: 830 2211 2382 2748 2778 2808 2865 3003 4547
Size: 1381 171 366 30 30 57 138 1544
Cuts at: 4547 830
Size: 3478
Fragments arranged by size:
3478 1544 1381 366 171 138 57 30
30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7195
PvuII CAG'CTG
Cuts at: 2368 2377 2422 2782 2866 4243 2368
Size: 9 45 360 84 1377 5320
Fragments arranged by size:
5320 1377 360 84 45 9
SacI G_AGCT'C
Cuts at: 3726 4076 4125 3726
Size: 350 49 6796
Fragments arranged by size:
6796 350 49
SacII CC_GC'GG
Cuts at: 2578 2578
Size: 7195
SalI G'TCGA_C
Cuts at: 1936 4537 1936
Size: 2601 4594
SanDI GG'GwC_CC
Cuts at: 3533 3533
Size: 7195
SbfI CC_TGCA'GG
Cuts at: 2211 3003 4547 2211
Size: 792 1544 4859
Fragments arranged by size:
4859 1544 792
ScaI AGT'ACT
Cuts at: 5443 7155 5443
Size: 1712 5483
SfcI C'TryA_G
Cuts at: 826 1045 2207 2378 2744 2774 2804 2861 2999
Size: 219 1162 171 366 30 30 57 138
Cuts at: 2999 4543 5678 6359 6550 826
Size: 1544 1135 681 191 1471
Fragments arranged by size:
1544 1471 1162 1135 681 366 219 191
171 138 57 30 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 2360 3446 3809 2360
Size: 1086 363 5746
Fragments arranged by size:
5746 1086 363
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7195
SgrAI Cr'CCGG_yG
Cuts at: 1700 3518 1700
Size: 1818 5377
SmaI CCC'GGG
Cuts at: 1872 2279 2479 4522 1872
Size: 407 200 2043 4545
Fragments arranged by size:
4545 2043 407 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 5299 6200 6462 820
Size: 197 926 1640 1716 901 262 1553
Fragments arranged by size:
1716 1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4986 493
Size: 4493 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7195
SphI G_CATG'C
Cuts at: 1132 4553 1132
Size: 3421 3774
SspI AAT'ATT
Cuts at: 5 52 5119 5
Size: 47 5067 2081
Fragments arranged by size:
5067 2081 47
StuI AGG'CCT
Cuts at: 1517 2969 4281 1517
Size: 1452 1312 4431
Fragments arranged by size:
4431 1452 1312
StyI C'CwwG_G
Cuts at: 513 1065 2004 3261 3444 3924 3930 4263 4634
Size: 552 939 1257 183 480 6 333 371
Cuts at: 4634 513
Size: 3074
Fragments arranged by size:
3074 1257 939 552 480 371 333 183
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4026 4408 5222 5239 5392 5577 6022
Size: 1255 2446 382 814 17 153 185 445
Cuts at: 6022 325
Size: 1498
Fragments arranged by size:
2446 1498 1255 814 445 382 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5441 7153 96
Size: 274 80 33 51 1443 3464 1712 138
Fragments arranged by size:
3464 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7155 7187 7155
Size: 32 7163
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7195
XbaI T'CTAG_A
Cuts at: 4531 4531
Size: 7195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3680 3680
Size: 7195
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7195
XmaI C'CCGG_G
Cuts at: 1870 2277 2477 4520 1870
Size: 407 200 2043 4545
Fragments arranged by size:
4545 2043 407 200
XmnI GAAnn'nnTTC
Cuts at: 2195 4436 5324 2195
Size: 2241 888 4066
Fragments arranged by size:
4066 2241 888
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5003 276
Size: 53 83 186 936 3469 2468
Fragments arranged by size:
3469 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoRV FalI FseI KasI NarI NruI PacI PspXI
RsrII SapI SexAI SfoI SrfI SwaI