Restriction map for
pFN21AE1481
(Circular) (Six-base) MAPSORT of: pFN21AE1481.seq Check: 151 from: 1 to: 5734
pFN21AE1481 5734 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2355 2355
Size: 5734
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 2630 2660 2990 3797 5181 5449 1632
Size: 840 158 30 330 807 1384 268 1917
Fragments arranged by size:
1917 1384 840 807 330 268 158 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2393 1184
Size: 1209 4525
AlwNI CAG_nnn'CTG
Cuts at: 2968 3477 5049 2968
Size: 509 1572 3653
Fragments arranged by size:
3653 1572 509
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 2918 3043 3269 3421 5530 1493
Size: 1425 125 226 152 2109 1697
Fragments arranged by size:
2109 1697 1425 226 152 125
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2528 3059 1870
Size: 73 585 531 4545
Fragments arranged by size:
4545 585 531 73
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3055 4510 618
Size: 325 217 627 1268 1455 1842
Fragments arranged by size:
1842 1455 1268 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3000 3053 1391
Size: 1609 53 4072
Fragments arranged by size:
4072 1609 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 928
Size: 971 135 4628
Fragments arranged by size:
4628 971 135
BbvCI CC'TCA_GC
Cuts at: 2943 2943
Size: 5734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3925 3959 1903
Size: 34 1988 34 3678
Fragments arranged by size:
3678 1988 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 2538 1548
Size: 990 4744
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 136
Size: 107 122 71 631 605 2673 1525
Fragments arranged by size:
2673 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2389 3795 4951 1790
Size: 599 1406 1156 2573
Fragments arranged by size:
2573 1406 1156 599
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2710 4394 1239
Size: 1007 464 1684 2579
Fragments arranged by size:
2579 1684 1007 464
Bpu10I CC'TnA_GC
Cuts at: 2604 2943 2994 2604
Size: 339 51 5344
Fragments arranged by size:
5344 339 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 3859 4724 5022 2342
Size: 1517 865 298 3054
Fragments arranged by size:
3054 1517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2799 4397 882
Size: 617 1300 1598 2219
Fragments arranged by size:
2219 1598 1300 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 2370 5728 2370
Size: 3358 2376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5509
Fragments arranged by size:
5509 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2942 2945 1186
Size: 913 843 3 3975
Fragments arranged by size:
3975 913 843 3
BseYI C'CCAG_C
Cuts at: 3000 4937 5433 3000
Size: 1937 496 3301
Fragments arranged by size:
3301 1937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2531 2906 1163
Size: 573 795 375 3991
Fragments arranged by size:
3991 795 573 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3795 3880 4951 3795
Size: 85 1071 4578
Fragments arranged by size:
4578 1071 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2389 3000 3053 3795 3880 4951 1391
Size: 399 599 611 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 599 399 85 53
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 2418 2817 3089 844
Size: 1511 63 399 272 3489
Fragments arranged by size:
3489 1511 399 272 63
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2203 2741 4229 4403 65
Size: 1183 955 538 1488 174 1396
Fragments arranged by size:
1488 1396 1183 955 538 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2662 3064 3431 3816 3833 5285 5371 5383
Size: 690 402 367 385 17 1452 86 12
Cuts at: 5383 5729 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 690 402 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2124 2759 2124
Size: 635 5099
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 3250 4044 4064 3250
Size: 794 20 4920
Fragments arranged by size:
4920 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DraIII CAC_nnn'GTG
Cuts at: 2512 2554 2512
Size: 42 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2320 4741 809
Size: 507 1004 2421 1802
Fragments arranged by size:
2421 1802 1004 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2775 2793 3181
Size: 54 1425 50 275 18 945 18 388
Cuts at: 3181 4070 5699 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 945 889 388 275 54 50
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2109 3664 1353
Size: 705 51 1555 3423
Fragments arranged by size:
3423 1555 705 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2472 2630 2660 2710 2990 3797
Size: 393 614 226 158 30 50 280 807
Cuts at: 3797 4394 5181 5449 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 807 787 614 597 393 280 268
226 158 50 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2912 2150
Size: 762 4972
EcoO109I rG'GnC_Cy
Cuts at: 2242 2878 2242
Size: 636 5098
EcoRI G'AATT_C
Cuts at: 2918 3043 3421 2918
Size: 125 378 5231
Fragments arranged by size:
5231 378 125
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4375 4407 4449 4481 1373
Size: 32 135 32 2803 32 42 32 2626
Fragments arranged by size:
2803 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2798 748
Size: 2050 3684
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4848 5032 1305
Size: 3543 184 2007
Fragments arranged by size:
3543 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2777 2795 10
Size: 54 1425 343 945 18 2949
Fragments arranged by size:
2949 1425 945 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2214 2507 3692 4051 4210 518
Size: 648 1048 293 1185 359 159 2042
Fragments arranged by size:
2042 1185 1048 648 359 293 159
MspA1I CmG'CkG
Cuts at: 1606 2575 3150 3827 4975 5220 1606
Size: 969 575 677 1148 245 2120
Fragments arranged by size:
2120 1148 969 677 575 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5182
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2638 3092 4637 1132
Size: 97 1409 454 1545 2229
Fragments arranged by size:
2229 1545 1409 454 97
PasI CC'CwG_GG
Cuts at: 1348 2587 1348
Size: 1239 4495
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2242
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 2433 3304 2433
Size: 871 4863
PstI C_TGCA'G
Cuts at: 830 2415 3086 830
Size: 1585 671 3478
Fragments arranged by size:
3478 1585 671
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2575 2575
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2098 2411 3082 4217 4898 5089 826
Size: 219 1053 313 671 1135 681 191 1471
Fragments arranged by size:
1471 1135 1053 681 671 313 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 3838 4739 5001 820
Size: 197 926 414 1481 901 262 1553
Fragments arranged by size:
1553 1481 926 901 414 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 3173 513
Size: 552 2108 3074
Fragments arranged by size:
3074 2108 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 3761 3778 3931 4116 4561 325
Size: 1255 777 1404 17 153 185 445 1498
Fragments arranged by size:
1498 1404 1255 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 2023 3070 2023
Size: 1047 4687
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI