Restriction map for
pFN21AE1479
(Circular) (Six-base) MAPSORT of: pFN21AE1479.seq Check: 2226 from: 1 to: 6478
pFN21AE1479 6478 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2274 2597 3392 2274
Size: 323 795 5360
Fragments arranged by size:
5360 795 323
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4288 278
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2832 3405 3821 1937
Size: 895 573 416 4594
Fragments arranged by size:
4594 895 573 416
Acc65I G'GTAC_C
Cuts at: 3799 3799
Size: 6478
AclI AA'CG_TT
Cuts at: 1563 4605 4978 1563
Size: 3042 373 3063
Fragments arranged by size:
3063 3042 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4541 5925 6193 1632
Size: 2909 1384 268 1917
Fragments arranged by size:
2909 1917 1384 268
AfeI AGC'GCT
Cuts at: 3195 3195
Size: 6478
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6281
AflIII A'CryG_T
Cuts at: 1203 2193 5377 6354 1203
Size: 990 3184 977 1327
Fragments arranged by size:
3184 1327 990 977
AgeI A'CCGG_T
Cuts at: 5309 5309
Size: 6478
AhdI GACnn_n'nnGTC
Cuts at: 5207 5207
Size: 6478
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6478
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2662 1184
Size: 1478 5000
AlwNI CAG_nnn'CTG
Cuts at: 2050 3622 4221 5793 2050
Size: 1572 599 1572 2735
Fragments arranged by size:
2735 1572 1572 599
ApaI G_GGCC'C
Cuts at: 2567 2655 3477 3655 2567
Size: 88 822 178 5390
Fragments arranged by size:
5390 822 178 88
ApaLI G'TGCA_C
Cuts at: 2302 4535 5691 2302
Size: 2233 1156 3089
Fragments arranged by size:
3089 2233 1156
ApoI r'AATT_y
Cuts at: 1493 3543 3787 4013 4165 6274 1493
Size: 2050 244 226 152 2109 1697
Fragments arranged by size:
2109 2050 1697 244 226 152
AscI GG'CGCG_CC
Cuts at: 2357 2357
Size: 6478
AseI AT'TA_AT
Cuts at: 160 1032 5032 160
Size: 872 4000 1606
Fragments arranged by size:
4000 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 2566 2792 3654 3677 3803 1870
Size: 73 528 95 226 862 23 126 4545
Fragments arranged by size:
4545 862 528 226 126 95 73 23
BamHI G'GATC_C
Cuts at: 3808 4175 3808
Size: 367 6111
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2467 2530 2585 3214
Size: 325 217 627 227 453 63 55 629
Cuts at: 3214 3308 3373 3432 3514 3799 5254 618
Size: 94 65 59 82 285 1455 1842
Fragments arranged by size:
1842 1455 629 627 453 325 285 227
217 94 82 65 63 59 55
BanII G_rGCy'C
Cuts at: 1391 2502 2567 2655 2775 3477 3512 3596 3655
Size: 1111 65 88 120 702 35 84 59
Cuts at: 3655 3797 1391
Size: 142 4072
Fragments arranged by size:
4072 1111 702 142 120 88 84 65
59 35
BbeI G_GCGC'C
Cuts at: 2018 2471 2534 3436 2018
Size: 453 63 902 5060
Fragments arranged by size:
5060 902 453 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2944 3305 3346 3523 928
Size: 971 1045 361 41 177 3883
Fragments arranged by size:
3883 1045 971 361 177 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2195 2229 4669 4703 1903
Size: 34 258 34 2440 34 3678
Fragments arranged by size:
3678 2440 258 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4370 5580 4370
Size: 1210 5268
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6478
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3059 5089 136
Size: 107 122 71 631 605 1387 2030 1525
Fragments arranged by size:
2030 1525 1387 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6473 6473
Size: 6478
BlpI GC'TnA_GC
Cuts at: 3895 3895
Size: 6478
Bme1580I G_kGCm'C
Cuts at: 1790 2306 2567 2655 3219 3378 3477 3519 3655
Size: 516 261 88 564 159 99 42 136
Cuts at: 3655 4539 5695 1790
Size: 884 1156 2573
Fragments arranged by size:
2573 1156 884 564 516 261 159 136
99 88 42
BmgBI CAC'GTC
Cuts at: 1318 2196 3692 1318
Size: 878 1496 4104
Fragments arranged by size:
4104 1496 878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4209 5167
Size: 416 53 83 399 24 1751 1041 958
Cuts at: 5167 442
Size: 1753
Fragments arranged by size:
1753 1751 1041 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2225 1056
Size: 1169 5309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2789 2948 3347 5138 1239
Size: 854 696 159 399 1791 2579
Fragments arranged by size:
2579 1791 854 696 399 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3092 4603 5468 5766 3092
Size: 1511 865 298 3804
Fragments arranged by size:
3804 1511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2320 2550 5141 882
Size: 617 821 230 2591 2219
Fragments arranged by size:
2591 2219 821 617 230
BsaAI yAC'GTr
Cuts at: 493 1204 2617 2911 3172 4269 493
Size: 711 1413 294 261 1097 2702
Fragments arranged by size:
2702 1413 1097 711 294 261
BsaBI GATnn'nnATC
Cuts at: 6472 6472
Size: 6478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2321 2468 2531
Size: 53 83 186 936 482 306 147 63
Cuts at: 2531 2658 3433 3579 4285 4667 5302 275
Size: 127 775 146 706 382 635 1451
Fragments arranged by size:
1451 936 775 706 635 482 382 306
186 147 146 127 83 63 53
BsaWI w'CCGG_w
Cuts at: 4911 5309 5583 5730 6141 4911
Size: 398 274 147 411 5248
Fragments arranged by size:
5248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6253
Fragments arranged by size:
6253 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6478
BseYI C'CCAG_C
Cuts at: 2185 3238 5681 6177 2185
Size: 1053 2443 496 2486
Fragments arranged by size:
2486 2443 1053 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 2926 1163
Size: 573 528 662 4715
Fragments arranged by size:
4715 662 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 2487 3468 3928 4689 5717
Size: 275 186 82 404 981 460 761 1028
Cuts at: 5717 1540
Size: 2301
Fragments arranged by size:
2301 1028 981 761 460 404 275 186
82
BsiHKAI G_wGCw'C
Cuts at: 2285 2306 2757 3596 4539 4624 5695 2285
Size: 21 451 839 943 85 1071 3068
Fragments arranged by size:
3068 1071 943 839 451 85 21
BsmI GAATG_Cn'
Cuts at: 3989 4082 3989
Size: 93 6385
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2306 2502 2567 2655 2757 2775
Size: 399 495 21 196 65 88 102 18
Cuts at: 2775 3219 3378 3477 3512 3519 3596 3655 3797
Size: 444 159 99 35 7 77 59 142
Cuts at: 3797 4539 4624 5695 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 444 399 196 159
142 102 99 88 85 77 65 59
35 21 18 7
BspEI T'CCGG_A
Cuts at: 6141 6141
Size: 6478
BspHI T'CATG_A
Cuts at: 3294 4367 6097 3294
Size: 1073 1730 3675
Fragments arranged by size:
3675 1730 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 2274 2597 3392 3833 844
Size: 1346 84 323 795 441 3489
Fragments arranged by size:
3489 1346 795 441 323 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2631 4365 1394
Size: 429 808 1734 3507
Fragments arranged by size:
3507 1734 808 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4973 5147 65
Size: 1183 3725 174 1396
Fragments arranged by size:
3725 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2337 2486 3260 5122 5309 1700
Size: 110 527 149 774 1862 187 2869
Fragments arranged by size:
2869 1862 774 527 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6478
BssHII G'CGCG_C
Cuts at: 1925 2357 2370 2543 2901 1925
Size: 432 13 173 358 5502
Fragments arranged by size:
5502 432 358 173 13
BssSI C'ACGA_G
Cuts at: 2632 3047 4538 5550 2632
Size: 415 1491 1012 3560
Fragments arranged by size:
3560 1491 1012 415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3702 794
Size: 2908 3570
BstBI TT'CG_AA
Cuts at: 5282 5282
Size: 6478
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6478
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2185 2011
Size: 174 6304
BstYI r'GATC_y
Cuts at: 1972 2133 3808 4175 4560 4577 6029 6115 6127
Size: 161 1675 367 385 17 1452 86 12
Cuts at: 6127 6473 1972
Size: 346 1977
Fragments arranged by size:
1977 1675 1452 385 367 346 161 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2146 2481 2493 2526 2534
Size: 552 939 6 136 335 12 33 8
Cuts at: 2534 3064 3468 3522 3723 513
Size: 530 404 54 201 3268
Fragments arranged by size:
3268 939 552 530 404 335 201 136
54 33 12 8 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5739
Fragments arranged by size:
5739 683 56
BtsI GCAGTG_nn'
Cuts at: 2587 3700 3994 4788 4808 2587
Size: 1113 294 794 20 4257
Fragments arranged by size:
4257 1113 794 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6443
DraI TTT'AAA
Cuts at: 3782 3782
Size: 6478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5485 809
Size: 507 4169 1802
Fragments arranged by size:
4169 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2080 2402
Size: 54 1425 50 275 18 177 73 322
Cuts at: 2402 2484 2709 2862 2979 3174 3465 3646 3925
Size: 82 225 153 117 195 291 181 279
Cuts at: 3925 4814 6443 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 322 291 279 275 225
195 181 177 153 117 82 73 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2080 2484 3465 3925 1537
Size: 275 268 404 981 460 4090
Fragments arranged by size:
4090 981 460 404 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 2618 3281 4408 1353
Size: 1265 663 1127 3423
Fragments arranged by size:
3423 1265 1127 663
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5065 5439 5585 387
Size: 1064 138 3476 374 146 1280
Fragments arranged by size:
3476 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3594 3594
Size: 6478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2789 2948 3347 4541 5138 5925
Size: 393 461 696 159 399 1194 597 787
Cuts at: 5925 6193 1239
Size: 268 1524
Fragments arranged by size:
1524 1194 787 696 597 461 399 393
268 159
EcoNI CCTnn'n_nnAGG
Cuts at: 3134 3134
Size: 6478
EcoO109I rG'GnC_Cy
Cuts at: 2509 2923 3094 3473 2509
Size: 414 171 379 5514
Fragments arranged by size:
5514 414 379 171
EcoRI G'AATT_C
Cuts at: 3787 4165 3787
Size: 378 6100
FseI GG_CCGG'CC
Cuts at: 2490 2490
Size: 6478
FspI TGC'GCA
Cuts at: 2638 3134 3460 4984 2638
Size: 496 326 1524 4132
Fragments arranged by size:
4132 1524 496 326
FspAI rTGC'GCAy
Cuts at: 2638 2638
Size: 6478
HaeII r_GCGC'y
Cuts at: 1678 2018 2033 2471 2534 2726 2800 3197 3436
Size: 340 15 438 63 192 74 397 239
Cuts at: 3436 5625 1678
Size: 2189 2531
Fragments arranged by size:
2531 2189 438 397 340 239 192 74
63 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 5119 5151 5193
Size: 32 135 32 728 32 2787 32 42
Cuts at: 5193 5225 1373
Size: 32 2626
Fragments arranged by size:
2787 2626 728 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3822 4068 669
Size: 988 281 1884 246 3079
Fragments arranged by size:
3079 1884 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6478
HpaI GTT'AAC
Cuts at: 4068 4068
Size: 6478
KasI G'GCGC_C
Cuts at: 2014 2467 2530 3432 2014
Size: 453 63 902 5060
Fragments arranged by size:
5060 902 453 63
KpnI G_GTAC'C
Cuts at: 3803 3803
Size: 6478
MfeI C'AATT_G
Cuts at: 4077 4077
Size: 6478
MluI A'CGCG_T
Cuts at: 6354 6354
Size: 6478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5592 5776 1305
Size: 4287 184 2007
Fragments arranged by size:
4287 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2981 3176 10
Size: 54 1425 343 1149 195 3312
Fragments arranged by size:
3312 1425 1149 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4436 4795 4954 518
Size: 648 843 2427 359 159 2042
Fragments arranged by size:
2427 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2148 2239 2495 2536 3464 3470 3644
Size: 441 101 91 256 41 928 6 174
Cuts at: 3644 3894 4571 5719 5964 1606
Size: 250 677 1148 245 2120
Fragments arranged by size:
2120 1148 928 677 441 256 250 245
174 101 91 41 6
NaeI GCC'GGC
Cuts at: 2339 2488 2339
Size: 149 6329
NarI GG'CG_CC
Cuts at: 2015 2468 2531 3433 2015
Size: 453 63 902 5060
Fragments arranged by size:
5060 902 453 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4987
Fragments arranged by size:
4987 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6478
NgoMIV G'CCGG_C
Cuts at: 2337 2486 2337
Size: 149 6329
NheI G'CTAG_C
Cuts at: 1052 2221 1052
Size: 1169 5309
NotI GC'GGCC_GC
Cuts at: 3465 3925 3465
Size: 460 6018
NspI r_CATG'y
Cuts at: 1132 1229 2176 2521 3836 5381 1132
Size: 97 947 345 1315 1545 2229
Fragments arranged by size:
2229 1545 1315 947 345 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6478
PciI A'CATG_T
Cuts at: 5377 5377
Size: 6478
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 2617 1108
Size: 1020 489 4969
Fragments arranged by size:
4969 1020 489
PfoI T'CCnGG_A
Cuts at: 2551 2916 2551
Size: 365 6113
PmeI GTTT'AAAC
Cuts at: 3782 3782
Size: 6478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4547 4579 6060 6092 4547
Size: 32 1481 32 4933
Fragments arranged by size:
4933 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3116 1613
Size: 1503 4975
PsiI TTA'TAA
Cuts at: 4048 4048
Size: 6478
PspOMI G'GGCC_C
Cuts at: 2563 2651 3473 3651 2563
Size: 88 822 178 5390
Fragments arranged by size:
5390 822 178 88
PstI C_TGCA'G
Cuts at: 830 3830 830
Size: 3000 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6478
PvuII CAG'CTG
Cuts at: 2047 3644 2047
Size: 1597 4881
SacI G_AGCT'C
Cuts at: 3596 3596
Size: 6478
SacII CC_GC'GG
Cuts at: 2149 2496 2537 3471 2149
Size: 347 41 934 5156
Fragments arranged by size:
5156 934 347 41
SalI G'TCGA_C
Cuts at: 1936 3820 1936
Size: 1884 4594
SapI GCTCTTCn'nnn_
Cuts at: 2618 2618
Size: 6478
SbfI CC_TGCA'GG
Cuts at: 3830 3830
Size: 6478
ScaI AGT'ACT
Cuts at: 4726 6438 4726
Size: 1712 4766
SexAI A'CCwGG_T
Cuts at: 2310 3014 3231 2310
Size: 704 217 5557
Fragments arranged by size:
5557 704 217
SfcI C'TryA_G
Cuts at: 826 1045 3826 4961 5642 5833 826
Size: 219 2781 1135 681 191 1471
Fragments arranged by size:
2781 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3059 3059
Size: 6478
SfoI GGC'GCC
Cuts at: 2016 2469 2532 3434 2016
Size: 453 63 902 5060
Fragments arranged by size:
5060 902 453 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6478
SmaI CCC'GGG
Cuts at: 1872 2568 3656 3679 3805 1872
Size: 696 1088 23 126 4545
Fragments arranged by size:
4545 1088 696 126 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3107 4582 5483 5745 820
Size: 197 926 1164 1475 901 262 1553
Fragments arranged by size:
1553 1475 1164 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4269 493
Size: 3776 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6478
SphI G_CATG'C
Cuts at: 1132 2176 2521 3836 1132
Size: 1044 345 1315 3774
Fragments arranged by size:
3774 1315 1044 345
SrfI GCCC'GGGC
Cuts at: 2568 3656 2568
Size: 1088 5390
SspI AAT'ATT
Cuts at: 5 52 4402 5
Size: 47 4350 2081
Fragments arranged by size:
4350 2081 47
StuI AGG'CCT
Cuts at: 1517 3665 1517
Size: 2148 4330
StyI C'CwwG_G
Cuts at: 513 1065 2004 3660 3917 513
Size: 552 939 1656 257 3074
Fragments arranged by size:
3074 1656 939 552 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2986 3574 4505 4522 4675 4860 5305
Size: 1255 1406 588 931 17 153 185 445
Cuts at: 5305 325
Size: 1498
Fragments arranged by size:
1498 1406 1255 931 588 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4724 6436 96
Size: 274 80 33 51 1443 2747 1712 138
Fragments arranged by size:
2747 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6438 6470 6438
Size: 32 6446
Tth111I GACn'n_nGTC
Cuts at: 1316 2312 1316
Size: 996 5482
XbaI T'CTAG_A
Cuts at: 3814 3814
Size: 6478
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6478
XmaI C'CCGG_G
Cuts at: 1870 2566 3654 3677 3803 1870
Size: 696 1088 23 126 4545
Fragments arranged by size:
4545 1088 696 126 23
XmnI GAAnn'nnTTC
Cuts at: 4607 4607
Size: 6478
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4286 276
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SanDI SwaI
XcmI