(Circular) (Six-base) MAPSORT of: pFN21AE1478.seq Check: 7014 from: 1 to: 6226 pFN21AE1478 6226 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2501 3087 3496 2501 Size: 586 409 5231 Fragments arranged by size: 5231 586 409 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4036 278 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 3417 3569 1937 Size: 436 1044 152 4594 Fragments arranged by size: 4594 1044 436 152 Acc65I G'GTAC_C Cuts at: 3547 3547 Size: 6226 AclI AA'CG_TT Cuts at: 1563 4353 4726 1563 Size: 2790 373 3063 Fragments arranged by size: 3063 2790 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3187 4289 5673 5941 1632 Size: 1555 1102 1384 268 1917 Fragments arranged by size: 1917 1555 1384 1102 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6029 AflIII A'CryG_T Cuts at: 1203 3143 5125 6102 1203 Size: 1940 1982 977 1327 Fragments arranged by size: 1982 1940 1327 977 AgeI A'CCGG_T Cuts at: 5057 5057 Size: 6226 AhdI GACnn_n'nnGTC Cuts at: 4955 4955 Size: 6226 AleI CACnn'nnGTG Cuts at: 1166 2225 2555 3122 3146 1166 Size: 1059 330 567 24 4246 Fragments arranged by size: 4246 1059 567 330 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6226 AlwNI CAG_nnn'CTG Cuts at: 2920 3969 5541 2920 Size: 1049 1572 3605 Fragments arranged by size: 3605 1572 1049 ApaI G_GGCC'C Cuts at: 2021 2021 Size: 6226 ApaLI G'TGCA_C Cuts at: 4283 5439 4283 Size: 1156 5070 ApoI r'AATT_y Cuts at: 1493 2785 3535 3761 3913 6022 1493 Size: 1292 750 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 750 226 152 AseI AT'TA_AT Cuts at: 160 1032 4780 160 Size: 872 3748 1606 Fragments arranged by size: 3748 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 AvaI C'yCGr_G Cuts at: 1870 1943 2200 2504 2586 3066 3551 1870 Size: 73 257 304 82 480 485 4545 Fragments arranged by size: 4545 485 480 304 257 82 73 BamHI G'GATC_C Cuts at: 3556 3923 3556 Size: 367 5859 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2110 2324 2500 2708 Size: 325 217 627 254 69 214 176 208 Cuts at: 2708 3547 5002 618 Size: 839 1455 1842 Fragments arranged by size: 1842 1455 839 627 325 254 217 214 208 176 69 BanII G_rGCy'C Cuts at: 1391 2021 2322 3545 1391 Size: 630 301 1223 4072 Fragments arranged by size: 4072 1223 630 301 BbeI G_GCGC'C Cuts at: 2045 2114 2045 Size: 69 6157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2278 2449 2983 3368 928 Size: 971 379 171 534 385 3786 Fragments arranged by size: 3786 971 534 385 379 171 BbvCI CC'TCA_GC Cuts at: 3401 3401 Size: 6226 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4417 4451 1903 Size: 34 2480 34 3678 Fragments arranged by size: 3678 2480 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4118 5328 4118 Size: 1210 5016 BclI T'GATC_A Cuts at: 1548 2634 2805 1548 Size: 1086 171 4969 Fragments arranged by size: 4969 1086 171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3454 4837 136 Size: 107 122 71 631 605 1782 1383 1525 Fragments arranged by size: 1782 1525 1383 631 605 122 107 71 BglII A'GATC_T Cuts at: 6221 6221 Size: 6226 BlpI GC'TnA_GC Cuts at: 3643 3643 Size: 6226 Bme1580I G_kGCm'C Cuts at: 1790 2021 2505 4287 5443 1790 Size: 231 484 1782 1156 2573 Fragments arranged by size: 2573 1782 1156 484 231 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6226 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3957 4915 442 Size: 416 53 83 399 24 2540 958 1753 Fragments arranged by size: 2540 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2039 1056 Size: 983 5243 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 3028 3446 4886 1239 Size: 1148 641 418 1440 2579 Fragments arranged by size: 2579 1440 1148 641 418 Bpu10I CC'TnA_GC Cuts at: 3273 3401 3273 Size: 128 6098 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2216 2381 2459 3299 4351 5216 5514 2216 Size: 165 78 840 1052 865 298 2928 Fragments arranged by size: 2928 1052 865 840 298 165 78 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4889 882 Size: 617 3390 2219 Fragments arranged by size: 3390 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2872 3144 4017 493 Size: 711 1668 272 873 2702 Fragments arranged by size: 2702 1668 873 711 272 BsaBI GATnn'nnATC Cuts at: 6220 6220 Size: 6226 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2111 2216 2283 Size: 53 83 186 936 509 69 105 67 Cuts at: 2283 2967 4033 4415 5050 275 Size: 684 1066 382 635 1451 Fragments arranged by size: 1451 1066 936 684 635 509 382 186 105 83 69 67 53 BsaWI w'CCGG_w Cuts at: 4659 5057 5331 5478 5889 4659 Size: 398 274 147 411 4996 Fragments arranged by size: 4996 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3068 3098 3155 3170 3185 Size: 30 165 30 1665 30 57 15 15 Cuts at: 3185 3200 1178 Size: 15 4204 Fragments arranged by size: 4204 1665 165 57 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2522 3104 3449 1186 Size: 883 453 582 345 3963 Fragments arranged by size: 3963 883 582 453 345 BseYI C'CCAG_C Cuts at: 2644 5429 5925 2644 Size: 2785 496 2945 Fragments arranged by size: 2945 2785 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5653 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 2556 3483 3676 4437 5465 Size: 275 186 51 504 927 193 761 1028 Cuts at: 5465 1540 Size: 2301 Fragments arranged by size: 2301 1028 927 761 504 275 193 186 51 BsiHKAI G_wGCw'C Cuts at: 2232 2322 4287 4372 5443 2232 Size: 90 1965 85 1071 3015 Fragments arranged by size: 3015 1965 1071 90 85 BsmI GAATG_Cn' Cuts at: 3737 3830 3737 Size: 93 6133 BsmBI CGTCTCn'nnnn_ Cuts at: 3355 3355 Size: 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2232 2322 2505 3545 4287 4372 Size: 399 231 211 90 183 1040 742 85 Cuts at: 4372 5443 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1040 742 399 231 211 183 90 85 BspEI T'CCGG_A Cuts at: 5889 5889 Size: 6226 BspHI T'CATG_A Cuts at: 4115 5845 4115 Size: 1730 4496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 2501 2832 3087 3496 3581 844 Size: 1634 23 331 255 409 85 3489 Fragments arranged by size: 3489 1634 409 331 255 85 23 BsrBI CCG'CTC Cuts at: 1394 1823 2482 3283 4113 1394 Size: 429 659 801 830 3507 Fragments arranged by size: 3507 830 801 659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4721 4895 65 Size: 1183 3473 174 1396 Fragments arranged by size: 3473 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2113 4870 5057 1700 Size: 110 234 69 2757 187 2869 Fragments arranged by size: 2869 2757 234 187 110 69 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6226 BssHII G'CGCG_C Cuts at: 1925 2029 2031 2301 1925 Size: 104 2 270 5850 Fragments arranged by size: 5850 270 104 2 BssSI C'ACGA_G Cuts at: 2522 3134 4286 5298 2522 Size: 612 1152 1012 3450 Fragments arranged by size: 3450 1152 1012 612 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6226 BstBI TT'CG_AA Cuts at: 5030 5030 Size: 6226 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6226 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2524 2620 2182 Size: 342 96 5788 Fragments arranged by size: 5788 342 96 BstYI r'GATC_y Cuts at: 1972 2686 3556 3923 4308 4325 5777 5863 5875 Size: 714 870 367 385 17 1452 86 12 Cuts at: 5875 6221 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 870 714 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2220 2934 2940 3369 513 Size: 552 939 216 714 6 429 3370 Fragments arranged by size: 3370 939 714 552 429 216 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2418 487 Size: 683 56 1192 4295 Fragments arranged by size: 4295 1192 683 56 BtsI GCAGTG_nn' Cuts at: 3742 4536 4556 3742 Size: 794 20 5412 Fragments arranged by size: 5412 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6191 DraI TTT'AAA Cuts at: 3530 3530 Size: 6226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5233 809 Size: 507 3917 1802 Fragments arranged by size: 3917 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2085 2172 Size: 54 1425 50 275 18 219 36 87 Cuts at: 2172 2553 2832 2937 3234 3673 4562 6191 8 Size: 381 279 105 297 439 889 1629 43 Fragments arranged by size: 1629 1425 889 439 381 297 279 275 219 105 87 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2049 2553 3673 1537 Size: 275 237 504 1120 4090 Fragments arranged by size: 4090 1120 504 275 237 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2489 3056 3341 4156 1353 Size: 705 431 567 285 815 3423 Fragments arranged by size: 3423 815 705 567 431 285 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2361 4813 5187 5333 387 Size: 1064 138 772 2452 374 146 1280 Fragments arranged by size: 2452 1280 1064 772 374 146 138 EcoICRI GAG'CTC Cuts at: 2320 2320 Size: 6226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 3028 3187 3446 4289 4886 5673 Size: 393 755 641 159 259 843 597 787 Cuts at: 5673 5941 1239 Size: 268 1524 Fragments arranged by size: 1524 843 787 755 641 597 393 268 259 159 EcoO109I rG'GnC_Cy Cuts at: 3027 3063 3027 Size: 36 6190 EcoRI G'AATT_C Cuts at: 3535 3913 3535 Size: 378 5848 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 3199 2500 Size: 699 5527 FspI TGC'GCA Cuts at: 4732 4732 Size: 6226 HaeII r_GCGC'y Cuts at: 1678 2045 2114 2261 5373 1678 Size: 367 69 147 3112 2531 Fragments arranged by size: 3112 2531 367 147 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3170 3202 4867 4899 4941 Size: 32 135 32 1598 32 1665 32 42 Cuts at: 4941 4973 1373 Size: 32 2626 Fragments arranged by size: 2626 1665 1598 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3570 3816 669 Size: 988 281 1632 246 3079 Fragments arranged by size: 3079 1632 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6226 HpaI GTT'AAC Cuts at: 3816 3816 Size: 6226 KasI G'GCGC_C Cuts at: 2041 2110 2041 Size: 69 6157 KpnI G_GTAC'C Cuts at: 3551 3551 Size: 6226 MfeI C'AATT_G Cuts at: 3825 3825 Size: 6226 MluI A'CGCG_T Cuts at: 6102 6102 Size: 6226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5340 5524 1305 Size: 4035 184 2007 Fragments arranged by size: 4035 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2834 3236 10 Size: 54 1425 343 342 660 402 3000 Fragments arranged by size: 3000 1425 660 402 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2210 2225 2555 3122 3146 3245 Size: 648 1014 30 15 330 567 24 99 Cuts at: 3245 3293 3506 4184 4543 4702 518 Size: 48 213 678 359 159 2042 Fragments arranged by size: 2042 1014 678 648 567 359 330 213 159 99 48 30 24 15 MspA1I CmG'CkG Cuts at: 1606 2146 2751 3405 3642 4319 5467 5712 1606 Size: 540 605 654 237 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 654 605 540 245 237 NaeI GCC'GGC Cuts at: 2046 2115 2046 Size: 69 6157 NarI GG'CG_CC Cuts at: 2042 2111 2042 Size: 69 6157 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4735 Fragments arranged by size: 4735 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6226 NgoMIV G'CCGG_C Cuts at: 2044 2113 2044 Size: 69 6157 NheI G'CTAG_C Cuts at: 1052 2035 1052 Size: 983 5243 NotI GC'GGCC_GC Cuts at: 3673 3673 Size: 6226 NspI r_CATG'y Cuts at: 1132 1229 2365 3584 5129 1132 Size: 97 1136 1219 1545 2229 Fragments arranged by size: 2229 1545 1219 1136 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6226 PciI A'CATG_T Cuts at: 5125 5125 Size: 6226 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6226 PfoI T'CCnGG_A Cuts at: 2592 2975 2592 Size: 383 5843 PmeI GTTT'AAAC Cuts at: 3530 3530 Size: 6226 PmlI CAC'GTG Cuts at: 3144 3144 Size: 6226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4295 4327 5808 5840 4295 Size: 32 1481 32 4681 Fragments arranged by size: 4681 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3027 3063 3027 Size: 36 6190 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6226 PsiI TTA'TAA Cuts at: 3796 3796 Size: 6226 PspOMI G'GGCC_C Cuts at: 2017 2017 Size: 6226 PstI C_TGCA'G Cuts at: 830 2145 3578 830 Size: 1315 1433 3478 Fragments arranged by size: 3478 1433 1315 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6226 SacI G_AGCT'C Cuts at: 2322 2322 Size: 6226 SalI G'TCGA_C Cuts at: 1936 3568 1936 Size: 1632 4594 SanDI GG'GwC_CC Cuts at: 3027 3063 3027 Size: 36 6190 SapI GCTCTTCn'nnn_ Cuts at: 2489 3056 2489 Size: 567 5659 SbfI CC_TGCA'GG Cuts at: 3578 3578 Size: 6226 ScaI AGT'ACT Cuts at: 4474 6186 4474 Size: 1712 4514 SfcI C'TryA_G Cuts at: 826 1045 2141 2566 2815 3574 4709 5390 5581 Size: 219 1096 425 249 759 1135 681 191 Cuts at: 5581 826 Size: 1471 Fragments arranged by size: 1471 1135 1096 759 681 425 249 219 191 SfoI GGC'GCC Cuts at: 2043 2112 2043 Size: 69 6157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 SgrAI Cr'CCGG_yG Cuts at: 1700 2044 1700 Size: 344 5882 SmaI CCC'GGG Cuts at: 1872 2588 3068 3553 1872 Size: 716 480 485 4545 Fragments arranged by size: 4545 716 485 480 SmlI C'TyrA_G Cuts at: 820 1017 1943 2231 2396 2474 3278 4330 5231 Size: 197 926 288 165 78 804 1052 901 Cuts at: 5231 5493 820 Size: 262 1553 Fragments arranged by size: 1553 1052 926 901 804 288 262 197 165 78 SnaBI TAC'GTA Cuts at: 493 4017 493 Size: 3524 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6226 SphI G_CATG'C Cuts at: 1132 3584 1132 Size: 2452 3774 SspI AAT'ATT Cuts at: 5 52 4150 5 Size: 47 4098 2081 Fragments arranged by size: 4098 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6226 StyI C'CwwG_G Cuts at: 513 1065 2004 3174 3665 513 Size: 552 939 1170 491 3074 Fragments arranged by size: 3074 1170 939 552 491 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3120 4253 4270 4423 4608 5053 325 Size: 1255 1540 1133 17 153 185 445 1498 Fragments arranged by size: 1540 1498 1255 1133 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 4472 6184 Size: 274 80 33 51 1443 462 2033 1712 Cuts at: 6184 96 Size: 138 Fragments arranged by size: 2033 1712 1443 462 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3068 3100 6186 6218 3068 Size: 32 3086 32 3076 Fragments arranged by size: 3086 3076 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6226 XbaI T'CTAG_A Cuts at: 2683 3562 2683 Size: 879 5347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2719 2860 2719 Size: 141 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6226 XmaI C'CCGG_G Cuts at: 1870 2586 3066 3551 1870 Size: 716 480 485 4545 Fragments arranged by size: 4545 716 485 480 XmnI GAAnn'nnTTC Cuts at: 4355 4355 Size: 6226 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4034 276 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SexAI SfiI SrfI SwaI