Restriction map for
pFN21AE1478
(Circular) (Six-base) MAPSORT of: pFN21AE1478.seq Check: 7014 from: 1 to: 6226
pFN21AE1478 6226 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2501 3087 3496 2501
Size: 586 409 5231
Fragments arranged by size:
5231 586 409
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4036 278
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 3417 3569 1937
Size: 436 1044 152 4594
Fragments arranged by size:
4594 1044 436 152
Acc65I G'GTAC_C
Cuts at: 3547 3547
Size: 6226
AclI AA'CG_TT
Cuts at: 1563 4353 4726 1563
Size: 2790 373 3063
Fragments arranged by size:
3063 2790 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3187 4289 5673 5941 1632
Size: 1555 1102 1384 268 1917
Fragments arranged by size:
1917 1555 1384 1102 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6029
AflIII A'CryG_T
Cuts at: 1203 3143 5125 6102 1203
Size: 1940 1982 977 1327
Fragments arranged by size:
1982 1940 1327 977
AgeI A'CCGG_T
Cuts at: 5057 5057
Size: 6226
AhdI GACnn_n'nnGTC
Cuts at: 4955 4955
Size: 6226
AleI CACnn'nnGTG
Cuts at: 1166 2225 2555 3122 3146 1166
Size: 1059 330 567 24 4246
Fragments arranged by size:
4246 1059 567 330 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6226
AlwNI CAG_nnn'CTG
Cuts at: 2920 3969 5541 2920
Size: 1049 1572 3605
Fragments arranged by size:
3605 1572 1049
ApaI G_GGCC'C
Cuts at: 2021 2021
Size: 6226
ApaLI G'TGCA_C
Cuts at: 4283 5439 4283
Size: 1156 5070
ApoI r'AATT_y
Cuts at: 1493 2785 3535 3761 3913 6022 1493
Size: 1292 750 226 152 2109 1697
Fragments arranged by size:
2109 1697 1292 750 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4780 160
Size: 872 3748 1606
Fragments arranged by size:
3748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
AvaI C'yCGr_G
Cuts at: 1870 1943 2200 2504 2586 3066 3551 1870
Size: 73 257 304 82 480 485 4545
Fragments arranged by size:
4545 485 480 304 257 82 73
BamHI G'GATC_C
Cuts at: 3556 3923 3556
Size: 367 5859
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2110 2324 2500 2708
Size: 325 217 627 254 69 214 176 208
Cuts at: 2708 3547 5002 618
Size: 839 1455 1842
Fragments arranged by size:
1842 1455 839 627 325 254 217 214
208 176 69
BanII G_rGCy'C
Cuts at: 1391 2021 2322 3545 1391
Size: 630 301 1223 4072
Fragments arranged by size:
4072 1223 630 301
BbeI G_GCGC'C
Cuts at: 2045 2114 2045
Size: 69 6157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2278 2449 2983 3368 928
Size: 971 379 171 534 385 3786
Fragments arranged by size:
3786 971 534 385 379 171
BbvCI CC'TCA_GC
Cuts at: 3401 3401
Size: 6226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4417 4451 1903
Size: 34 2480 34 3678
Fragments arranged by size:
3678 2480 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4118 5328 4118
Size: 1210 5016
BclI T'GATC_A
Cuts at: 1548 2634 2805 1548
Size: 1086 171 4969
Fragments arranged by size:
4969 1086 171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3454 4837 136
Size: 107 122 71 631 605 1782 1383 1525
Fragments arranged by size:
1782 1525 1383 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6221 6221
Size: 6226
BlpI GC'TnA_GC
Cuts at: 3643 3643
Size: 6226
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2505 4287 5443 1790
Size: 231 484 1782 1156 2573
Fragments arranged by size:
2573 1782 1156 484 231
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3957 4915 442
Size: 416 53 83 399 24 2540 958 1753
Fragments arranged by size:
2540 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2039 1056
Size: 983 5243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 3028 3446 4886 1239
Size: 1148 641 418 1440 2579
Fragments arranged by size:
2579 1440 1148 641 418
Bpu10I CC'TnA_GC
Cuts at: 3273 3401 3273
Size: 128 6098
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2381 2459 3299 4351 5216 5514 2216
Size: 165 78 840 1052 865 298 2928
Fragments arranged by size:
2928 1052 865 840 298 165 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4889 882
Size: 617 3390 2219
Fragments arranged by size:
3390 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2872 3144 4017 493
Size: 711 1668 272 873 2702
Fragments arranged by size:
2702 1668 873 711 272
BsaBI GATnn'nnATC
Cuts at: 6220 6220
Size: 6226
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2111 2216 2283
Size: 53 83 186 936 509 69 105 67
Cuts at: 2283 2967 4033 4415 5050 275
Size: 684 1066 382 635 1451
Fragments arranged by size:
1451 1066 936 684 635 509 382 186
105 83 69 67 53
BsaWI w'CCGG_w
Cuts at: 4659 5057 5331 5478 5889 4659
Size: 398 274 147 411 4996
Fragments arranged by size:
4996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3068 3098 3155 3170 3185
Size: 30 165 30 1665 30 57 15 15
Cuts at: 3185 3200 1178
Size: 15 4204
Fragments arranged by size:
4204 1665 165 57 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2522 3104 3449 1186
Size: 883 453 582 345 3963
Fragments arranged by size:
3963 883 582 453 345
BseYI C'CCAG_C
Cuts at: 2644 5429 5925 2644
Size: 2785 496 2945
Fragments arranged by size:
2945 2785 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5653
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2556 3483 3676 4437 5465
Size: 275 186 51 504 927 193 761 1028
Cuts at: 5465 1540
Size: 2301
Fragments arranged by size:
2301 1028 927 761 504 275 193 186
51
BsiHKAI G_wGCw'C
Cuts at: 2232 2322 4287 4372 5443 2232
Size: 90 1965 85 1071 3015
Fragments arranged by size:
3015 1965 1071 90 85
BsmI GAATG_Cn'
Cuts at: 3737 3830 3737
Size: 93 6133
BsmBI CGTCTCn'nnnn_
Cuts at: 3355 3355
Size: 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2232 2322 2505 3545 4287 4372
Size: 399 231 211 90 183 1040 742 85
Cuts at: 4372 5443 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1040 742 399 231 211 183
90 85
BspEI T'CCGG_A
Cuts at: 5889 5889
Size: 6226
BspHI T'CATG_A
Cuts at: 4115 5845 4115
Size: 1730 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 2501 2832 3087 3496 3581 844
Size: 1634 23 331 255 409 85 3489
Fragments arranged by size:
3489 1634 409 331 255 85 23
BsrBI CCG'CTC
Cuts at: 1394 1823 2482 3283 4113 1394
Size: 429 659 801 830 3507
Fragments arranged by size:
3507 830 801 659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4721 4895 65
Size: 1183 3473 174 1396
Fragments arranged by size:
3473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2113 4870 5057 1700
Size: 110 234 69 2757 187 2869
Fragments arranged by size:
2869 2757 234 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6226
BssHII G'CGCG_C
Cuts at: 1925 2029 2031 2301 1925
Size: 104 2 270 5850
Fragments arranged by size:
5850 270 104 2
BssSI C'ACGA_G
Cuts at: 2522 3134 4286 5298 2522
Size: 612 1152 1012 3450
Fragments arranged by size:
3450 1152 1012 612
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6226
BstBI TT'CG_AA
Cuts at: 5030 5030
Size: 6226
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6226
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2524 2620 2182
Size: 342 96 5788
Fragments arranged by size:
5788 342 96
BstYI r'GATC_y
Cuts at: 1972 2686 3556 3923 4308 4325 5777 5863 5875
Size: 714 870 367 385 17 1452 86 12
Cuts at: 5875 6221 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 870 714 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2220 2934 2940 3369 513
Size: 552 939 216 714 6 429 3370
Fragments arranged by size:
3370 939 714 552 429 216 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2418 487
Size: 683 56 1192 4295
Fragments arranged by size:
4295 1192 683 56
BtsI GCAGTG_nn'
Cuts at: 3742 4536 4556 3742
Size: 794 20 5412
Fragments arranged by size:
5412 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6191
DraI TTT'AAA
Cuts at: 3530 3530
Size: 6226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5233 809
Size: 507 3917 1802
Fragments arranged by size:
3917 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2085 2172
Size: 54 1425 50 275 18 219 36 87
Cuts at: 2172 2553 2832 2937 3234 3673 4562 6191 8
Size: 381 279 105 297 439 889 1629 43
Fragments arranged by size:
1629 1425 889 439 381 297 279 275
219 105 87 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2049 2553 3673 1537
Size: 275 237 504 1120 4090
Fragments arranged by size:
4090 1120 504 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2489 3056 3341 4156 1353
Size: 705 431 567 285 815 3423
Fragments arranged by size:
3423 815 705 567 431 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2361 4813 5187 5333 387
Size: 1064 138 772 2452 374 146 1280
Fragments arranged by size:
2452 1280 1064 772 374 146 138
EcoICRI GAG'CTC
Cuts at: 2320 2320
Size: 6226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 3028 3187 3446 4289 4886 5673
Size: 393 755 641 159 259 843 597 787
Cuts at: 5673 5941 1239
Size: 268 1524
Fragments arranged by size:
1524 843 787 755 641 597 393 268
259 159
EcoO109I rG'GnC_Cy
Cuts at: 3027 3063 3027
Size: 36 6190
EcoRI G'AATT_C
Cuts at: 3535 3913 3535
Size: 378 5848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 3199 2500
Size: 699 5527
FspI TGC'GCA
Cuts at: 4732 4732
Size: 6226
HaeII r_GCGC'y
Cuts at: 1678 2045 2114 2261 5373 1678
Size: 367 69 147 3112 2531
Fragments arranged by size:
3112 2531 367 147 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3170 3202 4867 4899 4941
Size: 32 135 32 1598 32 1665 32 42
Cuts at: 4941 4973 1373
Size: 32 2626
Fragments arranged by size:
2626 1665 1598 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3570 3816 669
Size: 988 281 1632 246 3079
Fragments arranged by size:
3079 1632 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6226
HpaI GTT'AAC
Cuts at: 3816 3816
Size: 6226
KasI G'GCGC_C
Cuts at: 2041 2110 2041
Size: 69 6157
KpnI G_GTAC'C
Cuts at: 3551 3551
Size: 6226
MfeI C'AATT_G
Cuts at: 3825 3825
Size: 6226
MluI A'CGCG_T
Cuts at: 6102 6102
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5340 5524 1305
Size: 4035 184 2007
Fragments arranged by size:
4035 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2834 3236 10
Size: 54 1425 343 342 660 402 3000
Fragments arranged by size:
3000 1425 660 402 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2210 2225 2555 3122 3146 3245
Size: 648 1014 30 15 330 567 24 99
Cuts at: 3245 3293 3506 4184 4543 4702 518
Size: 48 213 678 359 159 2042
Fragments arranged by size:
2042 1014 678 648 567 359 330 213
159 99 48 30 24 15
MspA1I CmG'CkG
Cuts at: 1606 2146 2751 3405 3642 4319 5467 5712 1606
Size: 540 605 654 237 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 654 605 540 245 237
NaeI GCC'GGC
Cuts at: 2046 2115 2046
Size: 69 6157
NarI GG'CG_CC
Cuts at: 2042 2111 2042
Size: 69 6157
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4735
Fragments arranged by size:
4735 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6226
NgoMIV G'CCGG_C
Cuts at: 2044 2113 2044
Size: 69 6157
NheI G'CTAG_C
Cuts at: 1052 2035 1052
Size: 983 5243
NotI GC'GGCC_GC
Cuts at: 3673 3673
Size: 6226
NspI r_CATG'y
Cuts at: 1132 1229 2365 3584 5129 1132
Size: 97 1136 1219 1545 2229
Fragments arranged by size:
2229 1545 1219 1136 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6226
PciI A'CATG_T
Cuts at: 5125 5125
Size: 6226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6226
PfoI T'CCnGG_A
Cuts at: 2592 2975 2592
Size: 383 5843
PmeI GTTT'AAAC
Cuts at: 3530 3530
Size: 6226
PmlI CAC'GTG
Cuts at: 3144 3144
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5808 5840 4295
Size: 32 1481 32 4681
Fragments arranged by size:
4681 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3027 3063 3027
Size: 36 6190
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6226
PsiI TTA'TAA
Cuts at: 3796 3796
Size: 6226
PspOMI G'GGCC_C
Cuts at: 2017 2017
Size: 6226
PstI C_TGCA'G
Cuts at: 830 2145 3578 830
Size: 1315 1433 3478
Fragments arranged by size:
3478 1433 1315
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6226
SacI G_AGCT'C
Cuts at: 2322 2322
Size: 6226
SalI G'TCGA_C
Cuts at: 1936 3568 1936
Size: 1632 4594
SanDI GG'GwC_CC
Cuts at: 3027 3063 3027
Size: 36 6190
SapI GCTCTTCn'nnn_
Cuts at: 2489 3056 2489
Size: 567 5659
SbfI CC_TGCA'GG
Cuts at: 3578 3578
Size: 6226
ScaI AGT'ACT
Cuts at: 4474 6186 4474
Size: 1712 4514
SfcI C'TryA_G
Cuts at: 826 1045 2141 2566 2815 3574 4709 5390 5581
Size: 219 1096 425 249 759 1135 681 191
Cuts at: 5581 826
Size: 1471
Fragments arranged by size:
1471 1135 1096 759 681 425 249 219
191
SfoI GGC'GCC
Cuts at: 2043 2112 2043
Size: 69 6157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
SgrAI Cr'CCGG_yG
Cuts at: 1700 2044 1700
Size: 344 5882
SmaI CCC'GGG
Cuts at: 1872 2588 3068 3553 1872
Size: 716 480 485 4545
Fragments arranged by size:
4545 716 485 480
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 2396 2474 3278 4330 5231
Size: 197 926 288 165 78 804 1052 901
Cuts at: 5231 5493 820
Size: 262 1553
Fragments arranged by size:
1553 1052 926 901 804 288 262 197
165 78
SnaBI TAC'GTA
Cuts at: 493 4017 493
Size: 3524 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6226
SphI G_CATG'C
Cuts at: 1132 3584 1132
Size: 2452 3774
SspI AAT'ATT
Cuts at: 5 52 4150 5
Size: 47 4098 2081
Fragments arranged by size:
4098 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6226
StyI C'CwwG_G
Cuts at: 513 1065 2004 3174 3665 513
Size: 552 939 1170 491 3074
Fragments arranged by size:
3074 1170 939 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3120 4253 4270 4423 4608 5053 325
Size: 1255 1540 1133 17 153 185 445 1498
Fragments arranged by size:
1540 1498 1255 1133 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 4472 6184
Size: 274 80 33 51 1443 462 2033 1712
Cuts at: 6184 96
Size: 138
Fragments arranged by size:
2033 1712 1443 462 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 6186 6218 3068
Size: 32 3086 32 3076
Fragments arranged by size:
3086 3076 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6226
XbaI T'CTAG_A
Cuts at: 2683 3562 2683
Size: 879 5347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2719 2860 2719
Size: 141 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6226
XmaI C'CCGG_G
Cuts at: 1870 2586 3066 3551 1870
Size: 716 480 485 4545
Fragments arranged by size:
4545 716 485 480
XmnI GAAnn'nnTTC
Cuts at: 4355 4355
Size: 6226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4034 276
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI
PacI PspXI PsrI PvuII RsrII SacII SexAI SfiI
SrfI SwaI