Restriction map for
pFN21AE1477
(Circular) (Six-base) MAPSORT of: pFN21AE1477.seq Check: 6382 from: 1 to: 5758
pFN21AE1477 5758 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 2315 2799 3821 5205 5473 1632
Size: 543 140 484 1022 1384 268 1917
Fragments arranged by size:
1917 1384 1022 543 484 268 140
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2527 1166
Size: 1361 4397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2898 2930 2898
Size: 32 5726
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaLI G'TGCA_C
Cuts at: 2560 3815 4971 2560
Size: 1255 1156 3347
Fragments arranged by size:
3347 1255 1156
ApoI r'AATT_y
Cuts at: 1493 2021 2552 2805 3067 3293 3445 5554 1493
Size: 528 531 253 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 531 528 262 253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2997 3083 1870
Size: 73 1054 86 4545
Fragments arranged by size:
4545 1054 86 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2878 3079 4534 618
Size: 325 217 627 321 770 201 1455 1842
Fragments arranged by size:
1842 1455 770 627 325 321 217 201
BanII G_rGCy'C
Cuts at: 1391 2764 3077 1391
Size: 1373 313 4072
Fragments arranged by size:
4072 1373 313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2303 928
Size: 971 404 4383
Fragments arranged by size:
4383 971 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 2305 1548
Size: 757 5001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2406 5753 2406
Size: 3347 2411
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2564 2957 3819 4975 1790
Size: 774 393 862 1156 2573
Fragments arranged by size:
2573 1156 862 774 393
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2684 2410
Size: 274 5484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2771 4418 1239
Size: 1532 1647 2579
Fragments arranged by size:
2579 1647 1532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2398 4421 882
Size: 617 899 2023 2219
Fragments arranged by size:
2219 2023 899 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2673 2740 2759 2841 1186
Size: 1487 67 19 82 4103
Fragments arranged by size:
4103 1487 82 67 19
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2088 1163
Size: 573 352 4833
Fragments arranged by size:
4833 573 352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2564 3819 3904 4975 2564
Size: 1255 85 1071 3347
Fragments arranged by size:
3347 1255 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 3269 3362 2095
Size: 1174 93 4491
Fragments arranged by size:
4491 1174 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2564 2764 2957 3077 3819 3904 4975
Size: 399 774 200 193 120 742 85 1071
Cuts at: 4975 1391
Size: 2174
Fragments arranged by size:
2174 1071 774 742 399 200 193 120
85
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3113 844
Size: 2269 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2406 3088 3455 3840 3857 5309 5395 5407
Size: 434 682 367 385 17 1452 86 12
Cuts at: 5407 5753 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 682 434 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3019 513
Size: 552 1954 3252
Fragments arranged by size:
3252 1954 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2687 3274 4068 4088 2687
Size: 587 794 20 4357
Fragments arranged by size:
4357 794 587 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 2266 3062 2266
Size: 796 4962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723
Size: 54 1425 50 275 18 1375 889 1629
Cuts at: 5723 8
Size: 43
Fragments arranged by size:
1629 1425 1375 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2927 3688 1353
Size: 1574 761 3423
Fragments arranged by size:
3423 1574 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2315 2771 2799 3821 4418 5205
Size: 393 543 140 456 28 1022 597 787
Cuts at: 5205 5473 1239
Size: 268 1524
Fragments arranged by size:
1524 1022 787 597 543 456 393 268
140 28
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
EcoRV GAT'ATC
Cuts at: 2314 2314
Size: 5758
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2290 2322 4399 4431 4473
Size: 32 135 32 718 32 2077 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 2077 718 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 2711 4872 5056 1305
Size: 1032 374 2161 184 2007
Fragments arranged by size:
2161 2007 1032 374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2527 3716 4075 4234 518
Size: 648 1361 1189 359 159 2042
Fragments arranged by size:
2042 1361 1189 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2610 3174 3851 4999 5244 1606
Size: 1004 564 677 1148 245 2120
Fragments arranged by size:
2120 1148 1004 677 564 245
NcoI C'CATG_G
Cuts at: 513 1065 3019 513
Size: 552 1954 3252
Fragments arranged by size:
3252 1954 552
NdeI CA'TA_TG
Cuts at: 387 2651 387
Size: 2264 3494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2861 1108
Size: 1753 4005
PfoI T'CCnGG_A
Cuts at: 2411 2902 2411
Size: 491 5267
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspXI vC'TCGA_Gb
Cuts at: 2997 2997
Size: 5758
PstI C_TGCA'G
Cuts at: 830 3110 830
Size: 2280 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2610 2610
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 2860 2860
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 3106 4241 4922 5113 826
Size: 219 2061 1135 681 191 1471
Fragments arranged by size:
2061 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2997 3862 4763 5025 820
Size: 197 926 1054 865 901 262 1553
Fragments arranged by size:
1553 1054 926 901 865 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 2367 3682 5
Size: 47 2315 1315 2081
Fragments arranged by size:
2315 2081 1315 47
StuI AGG'CCT
Cuts at: 1517 2938 1517
Size: 1421 4337
StyI C'CwwG_G
Cuts at: 513 1065 2094 2112 3019 3197 513
Size: 552 1029 18 907 178 3074
Fragments arranged by size:
3074 1029 907 552 178 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2315 3785 3802 3955 4140 4585 325
Size: 1255 735 1470 17 153 185 445 1498
Fragments arranged by size:
1498 1470 1255 735 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4004 5716 96
Size: 274 80 33 51 1443 2027 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2337 2369 5718 5750 2337
Size: 32 3349 32 2345
Fragments arranged by size:
3349 2345 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2409 2409
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 2997 1943
Size: 1054 4704
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI