Restriction map for
pFN21AE1475
(Circular) (Six-base) MAPSORT of: pFN21AE1475.seq Check: 7906 from: 1 to: 5197
pFN21AE1475 5197 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3007 278
Size: 53 83 186 936 1471 2468
Fragments arranged by size:
2468 1471 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2540 1937
Size: 603 4594
Acc65I G'GTAC_C
Cuts at: 2518 2518
Size: 5197
AclI AA'CG_TT
Cuts at: 1563 3324 3697 1563
Size: 1761 373 3063
Fragments arranged by size:
3063 1761 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3260 4644 4912 1632
Size: 1628 1384 268 1917
Fragments arranged by size:
1917 1628 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5000
AflIII A'CryG_T
Cuts at: 1203 4096 5073 1203
Size: 2893 977 1327
Fragments arranged by size:
2893 1327 977
AgeI A'CCGG_T
Cuts at: 4028 4028
Size: 5197
AhdI GACnn_n'nnGTC
Cuts at: 3926 3926
Size: 5197
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5197
AlwNI CAG_nnn'CTG
Cuts at: 2485 2940 4512 2485
Size: 455 1572 3170
Fragments arranged by size:
3170 1572 455
ApaLI G'TGCA_C
Cuts at: 3254 4410 3254
Size: 1156 4041
ApoI r'AATT_y
Cuts at: 1493 2506 2732 2884 4993 1493
Size: 1013 226 152 2109 1697
Fragments arranged by size:
2109 1697 1013 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3751 160
Size: 872 2719 1606
Fragments arranged by size:
2719 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5197
AvaI C'yCGr_G
Cuts at: 1870 1943 2522 1870
Size: 73 579 4545
Fragments arranged by size:
4545 579 73
BamHI G'GATC_C
Cuts at: 2086 2527 2894 2086
Size: 441 367 4389
Fragments arranged by size:
4389 441 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2518 3973 618
Size: 325 217 627 731 1455 1842
Fragments arranged by size:
1842 1455 731 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 2516 1391
Size: 838 287 4072
Fragments arranged by size:
4072 838 287
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3388 3422 1903
Size: 34 1451 34 3678
Fragments arranged by size:
3678 1451 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3089 4299 3089
Size: 1210 3987
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5197
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2406 3808 136
Size: 107 122 71 631 605 734 1402 1525
Fragments arranged by size:
1525 1402 734 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5192 5192
Size: 5197
BlpI GC'TnA_GC
Cuts at: 2614 2614
Size: 5197
Bme1580I G_kGCm'C
Cuts at: 1790 2418 3258 4414 1790
Size: 628 840 1156 2573
Fragments arranged by size:
2573 1156 840 628
BmgBI CAC'GTC
Cuts at: 1318 2273 1318
Size: 955 4242
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2928 3886 442
Size: 416 53 83 399 24 1511 958 1753
Fragments arranged by size:
1753 1511 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3857 1239
Size: 2618 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3322 4187 4485 3322
Size: 865 298 4034
Fragments arranged by size:
4034 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2441 3860 882
Size: 617 942 1419 2219
Fragments arranged by size:
2219 1419 942 617
BsaAI yAC'GTr
Cuts at: 493 1204 2988 493
Size: 711 1784 2702
Fragments arranged by size:
2702 1784 711
BsaBI GATnn'nnATC
Cuts at: 5191 5191
Size: 5197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2466 3004 3386
Size: 53 83 186 936 651 282 538 382
Cuts at: 3386 4021 275
Size: 635 1451
Fragments arranged by size:
1451 936 651 635 538 382 282 186
83 53
BsaWI w'CCGG_w
Cuts at: 3630 4028 4302 4449 4860 3630
Size: 398 274 147 411 3967
Fragments arranged by size:
3967 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4972
Fragments arranged by size:
4972 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 1186
Size: 1075 4122
BseYI C'CCAG_C
Cuts at: 4400 4896 4400
Size: 496 4701
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4624
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2647 3408 4436 1540
Size: 275 186 646 761 1028 2301
Fragments arranged by size:
2301 1028 761 646 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 3258 3343 4414 2229
Size: 1029 85 1071 3012
Fragments arranged by size:
3012 1071 1029 85
BsmI GAATG_Cn'
Cuts at: 2708 2801 2708
Size: 93 5104
BsmBI CGTCTCn'nnnn_
Cuts at: 2263 2263
Size: 5197
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2418 2516 3258 3343 4414 1391
Size: 399 439 189 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 189 98 85
BspEI T'CCGG_A
Cuts at: 4860 4860
Size: 5197
BspHI T'CATG_A
Cuts at: 3086 4816 3086
Size: 1730 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2552 844
Size: 1268 440 3489
Fragments arranged by size:
3489 1268 440
BsrBI CCG'CTC
Cuts at: 1394 1823 3084 1394
Size: 429 1261 3507
Fragments arranged by size:
3507 1261 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3692 3866 65
Size: 1183 2444 174 1396
Fragments arranged by size:
2444 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3841 4028 1700
Size: 110 2031 187 2869
Fragments arranged by size:
2869 2031 187 110
BsrGI T'GTAC_A
Cuts at: 96 2334 96
Size: 2238 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5197
BssSI C'ACGA_G
Cuts at: 3257 4269 3257
Size: 1012 4185
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2485 794
Size: 1691 3506
BstBI TT'CG_AA
Cuts at: 4001 4001
Size: 5197
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5197
BstYI r'GATC_y
Cuts at: 1972 2086 2527 2894 3279 3296 4748 4834 4846
Size: 114 441 367 385 17 1452 86 12
Cuts at: 4846 5192 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 441 385 367 346 114 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2322 2322
Size: 5197
BtgI C'CryG_G
Cuts at: 513 1065 2028 513
Size: 552 963 3682
Fragments arranged by size:
3682 963 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4458
Fragments arranged by size:
4458 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 2713 3507 3527 2142
Size: 571 794 20 3812
Fragments arranged by size:
3812 794 571 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5197
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2254 2289 529
Size: 35 1690 35 3437
Fragments arranged by size:
3437 1690 35 35
DraI TTT'AAA
Cuts at: 2501 2501
Size: 5197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4204 809
Size: 507 2888 1802
Fragments arranged by size:
2888 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2275 2644
Size: 54 1425 50 275 18 201 244 369
Cuts at: 2644 3533 5162 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 369 275 244 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2644 1537
Size: 275 832 4090
Fragments arranged by size:
4090 832 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2236 3127 1353
Size: 773 110 891 3423
Fragments arranged by size:
3423 891 773 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2378 3784 4158 4304 387
Size: 1064 138 789 1406 374 146 1280
Fragments arranged by size:
1406 1280 1064 789 374 146 138
EcoICRI GAG'CTC
Cuts at: 2227 2227
Size: 5197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3260 3857 4644 4912 1239
Size: 393 1628 597 787 268 1524
Fragments arranged by size:
1628 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2325 2429 2325
Size: 104 5093
EcoO109I rG'GnC_Cy
Cuts at: 2281 2482 2281
Size: 201 4996
EcoRI G'AATT_C
Cuts at: 2506 2884 2506
Size: 378 4819
FspI TGC'GCA
Cuts at: 3703 3703
Size: 5197
HaeII r_GCGC'y
Cuts at: 1678 2021 2401 4344 1678
Size: 343 380 1943 2531
Fragments arranged by size:
2531 1943 380 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3838 3870 3912 3944 1373
Size: 32 135 32 2266 32 42 32 2626
Fragments arranged by size:
2626 2266 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2541 2787 669
Size: 988 281 603 246 3079
Fragments arranged by size:
3079 988 603 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5197
HpaI GTT'AAC
Cuts at: 2787 2787
Size: 5197
KpnI G_GTAC'C
Cuts at: 2522 2522
Size: 5197
MfeI C'AATT_G
Cuts at: 2796 2796
Size: 5197
MluI A'CGCG_T
Cuts at: 5073 5073
Size: 5197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4311 4495 1305
Size: 3006 184 2007
Fragments arranged by size:
3006 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3375
Fragments arranged by size:
3375 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3155 3514 3673 518
Size: 648 1989 359 159 2042
Fragments arranged by size:
2042 1989 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2613 3290 4438 4683 1606
Size: 424 583 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 583 424 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4645
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5197
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5197
NotI GC'GGCC_GC
Cuts at: 2644 2644
Size: 5197
NspI r_CATG'y
Cuts at: 1132 1229 2479 2555 4100 1132
Size: 97 1250 76 1545 2229
Fragments arranged by size:
2229 1545 1250 97 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5197
PciI A'CATG_T
Cuts at: 4096 4096
Size: 5197
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4295
PmeI GTTT'AAAC
Cuts at: 2501 2501
Size: 5197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 4779 4811 3266
Size: 32 1481 32 3652
Fragments arranged by size:
3652 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2281
Size: 5197
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5197
PsiI TTA'TAA
Cuts at: 2767 2767
Size: 5197
PstI C_TGCA'G
Cuts at: 830 2043 2349 2549 830
Size: 1213 306 200 3478
Fragments arranged by size:
3478 1213 306 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5197
SacI G_AGCT'C
Cuts at: 2229 2229
Size: 5197
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 5197
SalI G'TCGA_C
Cuts at: 1936 2539 1936
Size: 603 4594
SanDI GG'GwC_CC
Cuts at: 2281 2281
Size: 5197
SbfI CC_TGCA'GG
Cuts at: 2043 2549 2043
Size: 506 4691
ScaI AGT'ACT
Cuts at: 3445 5157 3445
Size: 1712 3485
SfcI C'TryA_G
Cuts at: 826 1045 2039 2345 2371 2545 3680 4361 4552
Size: 219 994 306 26 174 1135 681 191
Cuts at: 4552 826
Size: 1471
Fragments arranged by size:
1471 1135 994 681 306 219 191 174
26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5197
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5197
SmaI CCC'GGG
Cuts at: 1872 2524 1872
Size: 652 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3301 4202 4464 820
Size: 197 926 1358 901 262 1553
Fragments arranged by size:
1553 1358 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2988 493
Size: 2495 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5197
SphI G_CATG'C
Cuts at: 1132 2555 1132
Size: 1423 3774
SspI AAT'ATT
Cuts at: 5 52 3121 5
Size: 47 3069 2081
Fragments arranged by size:
3069 2081 47
StuI AGG'CCT
Cuts at: 1517 2331 2369 1517
Size: 814 38 4345
Fragments arranged by size:
4345 814 38
StyI C'CwwG_G
Cuts at: 513 1065 2451 2636 513
Size: 552 1386 185 3074
Fragments arranged by size:
3074 1386 552 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3224 3241 3394 3579 4024 325
Size: 1255 1644 17 153 185 445 1498
Fragments arranged by size:
1644 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 2441 3443
Size: 274 80 33 51 1443 357 107 1002
Cuts at: 3443 5155 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1002 357 274 138 107 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5157 5189 5157
Size: 32 5165
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5197
XbaI T'CTAG_A
Cuts at: 2533 2533
Size: 5197
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5197
XmaI C'CCGG_G
Cuts at: 1870 2522 1870
Size: 652 4545
XmnI GAAnn'nnTTC
Cuts at: 3326 3326
Size: 5197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3005 276
Size: 53 83 186 936 1471 2468
Fragments arranged by size:
2468 1471 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SapI SexAI SfiI SfoI SrfI SwaI
XcmI