Restriction map for
pFN21AE1473
(Circular) (Six-base) MAPSORT of: pFN21AE1473.seq Check: 4703 from: 1 to: 5848
pFN21AE1473 5848 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2426 3045 3911 5295 5563 1632
Size: 794 619 866 1384 268 1917
Fragments arranged by size:
1917 1384 866 794 619 268
AflII C'TTAA_G
Cuts at: 820 1017 2249 820
Size: 197 1232 4419
Fragments arranged by size:
4419 1232 197
AflIII A'CryG_T
Cuts at: 1203 3090 3092 4747 5724 1203
Size: 1887 2 1655 977 1327
Fragments arranged by size:
1887 1655 1327 977 2
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 3591 5163 3591
Size: 1572 4276
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2217 2317 2446 2471 2670 3157 3383 3535
Size: 724 100 129 25 199 487 226 152
Cuts at: 3535 5644 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 724 487 226 199 152 129
100 25
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2171 3173 1870
Size: 73 105 123 1002 4545
Fragments arranged by size:
4545 1002 123 105 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2361 2394 2361
Size: 33 5815
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 3169 4624 618
Size: 325 217 627 515 867 1455 1842
Fragments arranged by size:
1842 1455 867 627 515 325 217
BanII G_rGCy'C
Cuts at: 1391 3167 1391
Size: 1776 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3068 928
Size: 971 1169 3708
Fragments arranged by size:
3708 1169 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2170 3740 4950 2170
Size: 1570 1210 3068
Fragments arranged by size:
3068 1570 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BfrBI ATG'CAT
Cuts at: 2759 2759
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2253 2311 4459
Size: 107 122 71 631 605 581 58 2148
Cuts at: 4459 136
Size: 1525
Fragments arranged by size:
2148 1525 631 605 581 122 107 71
58
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2031 3090 3909 5065 1790
Size: 241 1059 819 1156 2573
Fragments arranged by size:
2573 1156 1059 819 241
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 4537 442
Size: 416 53 83 399 24 2162 958 1753
Fragments arranged by size:
2162 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4508 1239
Size: 3269 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2964 3973 4838 5136 2964
Size: 1009 865 298 3676
Fragments arranged by size:
3676 1009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 3639 493
Size: 711 1889 546 2702
Fragments arranged by size:
2702 1889 711 546
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 4998
BseYI C'CCAG_C
Cuts at: 2139 5051 5547 2139
Size: 2912 496 2440
Fragments arranged by size:
2912 2440 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2683 1163
Size: 573 947 4328
Fragments arranged by size:
4328 947 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2021 2097 3298 4059 5087 1540
Size: 275 186 20 76 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186 76 20
BsiHKAI G_wGCw'C
Cuts at: 2793 3909 3994 5065 2793
Size: 1116 85 1071 3576
Fragments arranged by size:
3576 1116 1071 85
BsmI GAATG_Cn'
Cuts at: 2855 3359 3452 2855
Size: 504 93 5251
Fragments arranged by size:
5251 504 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2793 3090 3167 3909 3994 5065
Size: 399 241 762 297 77 742 85 1071
Cuts at: 5065 1391
Size: 2174
Fragments arranged by size:
2174 1071 762 742 399 297 241 85
77
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3025 3737 5467 3025
Size: 712 1730 3406
Fragments arranged by size:
3406 1730 712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3203 844
Size: 2359 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 4492 4679 1700
Size: 110 282 2400 187 2869
Fragments arranged by size:
2869 2400 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 3178 3545 3930 3947 5399 5485 5497 5843
Size: 1206 367 385 17 1452 86 12 346
Cuts at: 5843 1972
Size: 1977
Fragments arranged by size:
1977 1452 1206 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2031 3132 513
Size: 552 966 1101 3229
Fragments arranged by size:
3229 1101 966 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3364 4158 4178 3364
Size: 794 20 5034
Fragments arranged by size:
5034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 2451 3152 2451
Size: 701 5147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 2094 3295
Size: 54 1425 50 275 18 188 76 1201
Cuts at: 3295 4184 5813 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1201 889 275 188 76 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2018 2094 3295 1537
Size: 275 206 76 1201 4090
Fragments arranged by size:
4090 1201 275 206 76
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 2401 3778 1353
Size: 918 130 1377 3423
Fragments arranged by size:
3423 1377 918 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 3045 3911 4508 5295 5563 1239
Size: 393 794 619 866 597 787 268 1524
Fragments arranged by size:
1524 866 794 787 619 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2878 2878
Size: 5848
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2430 2430
Size: 5848
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2039 4995 1678
Size: 361 2956 2531
Fragments arranged by size:
2956 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373
Size: 32 135 32 2917 32 42 32 2626
Fragments arranged by size:
2917 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3192 3438 669
Size: 988 281 1167 87 246 3079
Fragments arranged by size:
3079 1167 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 2206 748
Size: 1458 4390
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2942 4962 5146 1305
Size: 1637 2020 184 2007
Fragments arranged by size:
2020 2007 1637 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2848 3024 3806 4165 4324 518
Size: 648 1682 176 782 359 159 2042
Fragments arranged by size:
2042 1682 782 648 359 176 159
MspA1I CmG'CkG
Cuts at: 1606 2033 3264 3941 5089 5334 1606
Size: 427 1231 677 1148 245 2120
Fragments arranged by size:
2120 1231 1148 677 427 245
NaeI GCC'GGC
Cuts at: 2094 2094
Size: 5848
NcoI C'CATG_G
Cuts at: 513 1065 3132 513
Size: 552 2067 3229
Fragments arranged by size:
3229 2067 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NgoMIV G'CCGG_C
Cuts at: 2092 2092
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NsiI A_TGCA'T
Cuts at: 2761 2761
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2763 3206 4751 1132
Size: 97 1534 443 1545 2229
Fragments arranged by size:
2229 1545 1534 443 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PfoI T'CCnGG_A
Cuts at: 2500 3005 2500
Size: 505 5343
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PmlI CAC'GTG
Cuts at: 3093 3093
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2878 2878
Size: 5848
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PstI C_TGCA'G
Cuts at: 830 3200 830
Size: 2370 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
SacII CC_GC'GG
Cuts at: 2034 2034
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SexAI A'CCwGG_T
Cuts at: 2533 2533
Size: 5848
SfcI C'TryA_G
Cuts at: 826 1045 2922 3196 4331 5012 5203 826
Size: 219 1877 274 1135 681 191 1471
Fragments arranged by size:
1877 1471 1135 681 274 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2979 3952 4853 5115 820
Size: 197 926 306 730 973 901 262 1553
Fragments arranged by size:
1553 973 926 901 730 306 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2874 3132 3287 513
Size: 552 1809 258 155 3074
Fragments arranged by size:
3074 1809 552 258 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2804 2993 4094
Size: 274 80 33 51 1443 827 189 1101
Cuts at: 4094 5806 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1101 827 274 189 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 5808 5840 2372
Size: 32 3404 32 2380
Fragments arranged by size:
3404 2380 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3058 3977 3058
Size: 919 4929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI PacI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SfiI SfoI SrfI SwaI XcmI