Restriction map for
pFN21AE1472
(Circular) (Six-base) MAPSORT of: pFN21AE1472.seq Check: 3491 from: 1 to: 5620
pFN21AE1472 5620 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2166 2794 2963 1937
Size: 229 628 169 4594
Fragments arranged by size:
4594 628 229 169
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 2919 3747 4120 1563
Size: 1356 828 373 3063
Fragments arranged by size:
3063 1356 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 3683 5067 5335 1632
Size: 549 1502 1384 268 1917
Fragments arranged by size:
1917 1502 1384 549 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 3363 4935 3363
Size: 1572 4048
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2090 2180 2873 2929 3155 3307 5416 1493
Size: 597 90 693 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 693 597 226 152 90 56
AseI AT'TA_AT
Cuts at: 160 1032 2663 4174 160
Size: 872 1631 1511 1606
Fragments arranged by size:
1631 1606 1511 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
AvrII C'CTAG_G
Cuts at: 2909 2909
Size: 5620
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2039 2218 2939 1391
Size: 648 179 721 4072
Fragments arranged by size:
4072 721 648 179
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2900 928
Size: 971 1001 3648
Fragments arranged by size:
3648 1001 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 2559 1548
Size: 1011 4609
BfrBI ATG'CAT
Cuts at: 2803 2803
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 2058 3037 2058
Size: 979 4641
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4280 1239
Size: 3041 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2170 3411 493
Size: 711 966 1241 2702
Fragments arranged by size:
2702 1241 966 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2510 4053 4451 4725 4872 5283 2510
Size: 1543 398 274 147 411 2847
Fragments arranged by size:
2847 1543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5620
BseYI C'CCAG_C
Cuts at: 2032 4823 5319 2032
Size: 2791 496 2333
Fragments arranged by size:
2791 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2218 3681 3766 4837 2218
Size: 1463 85 1071 3001
Fragments arranged by size:
3001 1463 1071 85
BsmI GAATG_Cn'
Cuts at: 2626 3131 3224 2626
Size: 505 93 5022
Fragments arranged by size:
5022 505 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2218 2939 3681 3766 4837 1391
Size: 399 249 179 721 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 721 399 249 179 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2718 4115 4289 65
Size: 1183 1470 1397 174 1396
Fragments arranged by size:
1470 1397 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2507 2950 3317 3702 3719 5171 5257 5269
Size: 535 443 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 535 443 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2167 2795 2167
Size: 628 4992
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5068
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2185 2924 2185
Size: 739 4881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2114 4627 809
Size: 507 798 2513 1802
Fragments arranged by size:
2513 1802 798 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3067 3956 5585
Size: 54 1425 50 275 18 1237 889 1629
Cuts at: 5585 8
Size: 43
Fragments arranged by size:
1629 1425 1237 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2185 2817 3550 1353
Size: 832 632 733 3423
Fragments arranged by size:
3423 832 733 632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2216 2216
Size: 5620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 3683 4280 5067 5335 1239
Size: 393 549 1502 597 787 268 1524
Fragments arranged by size:
1524 1502 787 597 549 393 268
EcoRI G'AATT_C
Cuts at: 2090 2929 3307 2090
Size: 839 378 4403
Fragments arranged by size:
4403 839 378
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 2027 4767 1678
Size: 349 2740 2531
Fragments arranged by size:
2740 2531 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 2544 2576 2760
Size: 32 135 32 734 32 206 32 184
Cuts at: 2760 2792 4261 4293 4335 4367 1373
Size: 32 1469 32 42 32 2626
Fragments arranged by size:
2626 1469 734 206 184 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 2532 3219 2532
Size: 687 4933
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2560 2746 4734 4918 1305
Size: 1255 186 1988 184 2007
Fragments arranged by size:
2007 1988 1255 186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2679 3578 3937 4096 518
Size: 648 1513 899 359 159 2042
Fragments arranged by size:
2042 1513 899 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5068
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NsiI A_TGCA'T
Cuts at: 2805 2805
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2804 1108
Size: 1696 3924
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SacI G_AGCT'C
Cuts at: 2218 2218
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2085 2652 2968 4103 4784 4975 826
Size: 219 1040 567 316 1135 681 191 1471
Fragments arranged by size:
1471 1135 1040 681 567 316 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2170 3411 493
Size: 1677 1241 2702
Fragments arranged by size:
2702 1677 1241
SpeI A'CTAG_T
Cuts at: 152 2554 2860 152
Size: 2402 306 2912
Fragments arranged by size:
2912 2402 306
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 2431 2609 2667 3544 5
Size: 47 2379 178 58 877 2081
Fragments arranged by size:
2379 2081 877 178 58 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2632 2909 3059 513
Size: 552 1567 277 150 3074
Fragments arranged by size:
3074 1567 552 277 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3647 3664 3817 4002 4447 325
Size: 1255 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2690 3866 5578
Size: 274 80 33 51 1443 713 1176 1712
Cuts at: 5578 96
Size: 138
Fragments arranged by size:
1712 1443 1176 713 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 2772 1316
Size: 795 661 4164
Fragments arranged by size:
4164 795 661
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 2430 3749 2430
Size: 1319 4301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI