Restriction map for
pFN21AE1468
(Circular) (Six-base) MAPSORT of: pFN21AE1468.seq Check: 628 from: 1 to: 7546
pFN21AE1468 7546 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4629 4629
Size: 7546
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3552 5356 278
Size: 53 83 186 936 2016 1804 2468
Fragments arranged by size:
2468 2016 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2121 2874 2925 4889 1937
Size: 184 753 51 1964 4594
Fragments arranged by size:
4594 1964 753 184 51
Acc65I G'GTAC_C
Cuts at: 4867 4867
Size: 7546
AclI AA'CG_TT
Cuts at: 1563 5673 6046 1563
Size: 4110 373 3063
Fragments arranged by size:
4110 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2624 4007 4684 5609 6993 7261 1632
Size: 992 1383 677 925 1384 268 1917
Fragments arranged by size:
1917 1384 1383 992 925 677 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7349
AflIII A'CryG_T
Cuts at: 1203 2718 6445 7422 1203
Size: 1515 3727 977 1327
Fragments arranged by size:
3727 1515 1327 977
AgeI A'CCGG_T
Cuts at: 6377 6377
Size: 7546
AhdI GACnn_n'nnGTC
Cuts at: 6275 6275
Size: 7546
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4784 1184
Size: 3600 3946
AlwNI CAG_nnn'CTG
Cuts at: 3502 3720 3753 4259 4312 4321 5289 6861 3502
Size: 218 33 506 53 9 968 1572 4187
Fragments arranged by size:
4187 1572 968 506 218 53 33 9
ApaI G_GGCC'C
Cuts at: 3481 4096 4104 3481
Size: 615 8 6923
Fragments arranged by size:
6923 615 8
ApaLI G'TGCA_C
Cuts at: 3245 4774 5603 6759 3245
Size: 1529 829 1156 4032
Fragments arranged by size:
4032 1529 1156 829
ApoI r'AATT_y
Cuts at: 1493 4383 4855 5081 5233 7342 1493
Size: 2890 472 226 152 2109 1697
Fragments arranged by size:
2890 2109 1697 472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6100 160
Size: 872 5068 1606
Fragments arranged by size:
5068 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
AvaI C'yCGr_G
Cuts at: 1870 1943 3105 4545 4756 4871 1870
Size: 73 1162 1440 211 115 4545
Fragments arranged by size:
4545 1440 1162 211 115 73
BamHI G'GATC_C
Cuts at: 4876 5243 4876
Size: 367 7179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2561 2981 4747 4867
Size: 325 217 627 246 528 420 1766 120
Cuts at: 4867 6322 618
Size: 1455 1842
Fragments arranged by size:
1842 1766 1455 627 528 420 325 246
217 120
BanII G_rGCy'C
Cuts at: 1391 2117 2150 2456 2964 3044 3098 3114 3481
Size: 726 33 306 508 80 54 16 367
Cuts at: 3481 3918 3995 4096 4104 4135 4167 4481 4865
Size: 437 77 101 8 31 32 314 384
Cuts at: 4865 1391
Size: 4072
Fragments arranged by size:
4072 726 508 437 384 367 314 306
101 80 77 54 33 32 31 16
8
BbeI G_GCGC'C
Cuts at: 2565 2565
Size: 7546
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2093 3617 928
Size: 971 194 1524 4857
Fragments arranged by size:
4857 1524 971 194
BbvCI CC'TCA_GC
Cuts at: 3905 4113 4397 3905
Size: 208 284 7054
Fragments arranged by size:
7054 284 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2290 2324 4712 4746 5737 5771 1903
Size: 34 353 34 2388 34 991 34 3678
Fragments arranged by size:
3678 2388 991 353 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5438 6648 5438
Size: 1210 6336
BclI T'GATC_A
Cuts at: 1548 2347 2691 1548
Size: 799 344 6403
Fragments arranged by size:
6403 799 344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6157 136
Size: 107 122 71 631 605 4485 1525
Fragments arranged by size:
4485 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7541 7541
Size: 7546
BlpI GC'TnA_GC
Cuts at: 2044 4963 2044
Size: 2919 4627
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2201 2986 3249 3481 3967 4096 4104
Size: 248 163 785 263 232 486 129 8
Cuts at: 4104 4778 5607 6763 1790
Size: 674 829 1156 2573
Fragments arranged by size:
2573 1156 829 785 674 486 263 248
232 163 129 8
BmgBI CAC'GTC
Cuts at: 1318 2185 1318
Size: 867 6679
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2108 2528 3208
Size: 416 53 83 399 24 691 420 680
Cuts at: 3208 5277 6235 442
Size: 2069 958 1753
Fragments arranged by size:
2069 1753 958 691 680 420 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7546
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3830
Size: 7546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 2864 2978 3082 3524 3763 4127 4268
Size: 1565 60 114 104 442 239 364 141
Cuts at: 4268 4388 4507 4516 4825 4831 4853 6206 1239
Size: 120 119 9 309 6 22 1353 2579
Fragments arranged by size:
2579 1565 1353 442 364 309 239 141
120 119 114 104 60 22 9 6
Bpu10I CC'TnA_GC
Cuts at: 2511 2906 3043 3905 4113 4137 4397 4475 4480
Size: 395 137 862 208 24 260 78 5
Cuts at: 4480 2511
Size: 5577
Fragments arranged by size:
5577 862 395 260 208 137 78 24
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 3146 3806 5671 6536 6834 2150
Size: 996 660 1865 865 298 2862
Fragments arranged by size:
2862 1865 996 865 660 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3677 6209 882
Size: 617 2178 2532 2219
Fragments arranged by size:
2532 2219 2178 617
BsaAI yAC'GTr
Cuts at: 493 1204 2719 5337 493
Size: 711 1515 2618 2702
Fragments arranged by size:
2702 2618 1515 711
BsaBI GATnn'nnATC
Cuts at: 7540 7540
Size: 7546
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2090 2562 3549 3726
Size: 53 83 186 936 557 472 987 177
Cuts at: 3726 5353 5735 6370 275
Size: 1627 382 635 1451
Fragments arranged by size:
1627 1451 987 936 635 557 472 382
186 177 83 53
BsaWI w'CCGG_w
Cuts at: 3697 5979 6377 6651 6798 7209 3697
Size: 2282 398 274 147 411 4034
Fragments arranged by size:
4034 2282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 3939 3969 4397
Size: 30 165 30 1131 30 1375 30 428
Cuts at: 4397 4427 1178
Size: 30 4297
Fragments arranged by size:
4297 1375 1131 428 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3335 4130 4140 4176 4223 4385 1186
Size: 2149 795 10 36 47 162 4347
Fragments arranged by size:
4347 2149 795 162 47 36 10
BseYI C'CCAG_C
Cuts at: 2312 2963 3113 3709 3943 4068 4271 4353 4687
Size: 651 150 596 234 125 203 82 334
Cuts at: 4687 6749 7245 2312
Size: 2062 496 2613
Fragments arranged by size:
2613 2062 651 596 496 334 234 203
150 125 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2509 2558 2714 3357 3749 4607 1163
Size: 573 773 49 156 643 392 858 4102
Fragments arranged by size:
4102 858 773 643 573 392 156 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2660 4996 5757 6785 1540
Size: 275 186 659 2336 761 1028 2301
Fragments arranged by size:
2336 2301 1028 761 659 275 186
BsiHKAI G_wGCw'C
Cuts at: 2117 2456 3044 3249 3570 4481 4778 5607 5692
Size: 339 588 205 321 911 297 829 85
Cuts at: 5692 6763 2117
Size: 1071 2900
Fragments arranged by size:
2900 1071 911 829 588 339 321 297
205 85
BsmI GAATG_Cn'
Cuts at: 5057 5150 5057
Size: 93 7453
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2117 2150 2201 2456 2964 2986
Size: 399 248 79 33 51 255 508 22
Cuts at: 2986 3044 3098 3114 3249 3481 3570 3918 3967
Size: 58 54 16 135 232 89 348 49
Cuts at: 3967 3995 4096 4104 4135 4167 4481 4778 4865
Size: 28 101 8 31 32 314 297 87
Cuts at: 4865 5607 5692 6763 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 348 314 297
255 248 232 135 101 89 87 85
79 58 54 51 49 33 32 31
28 22 16 8
BspEI T'CCGG_A
Cuts at: 3697 7209 3697
Size: 3512 4034
BspHI T'CATG_A
Cuts at: 5435 7165 5435
Size: 1730 5816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4629 4776 4901 844
Size: 3785 147 125 3489
Fragments arranged by size:
3785 3489 147 125
BsrBI CCG'CTC
Cuts at: 1394 1823 3814 4285 5433 1394
Size: 429 1991 471 1148 3507
Fragments arranged by size:
3507 1991 1148 471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2711 6041 6215 65
Size: 1183 1463 3330 174 1396
Fragments arranged by size:
3330 1463 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2659 6190 6377 1700
Size: 110 849 3531 187 2869
Fragments arranged by size:
3531 2869 849 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7546
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7546
BssSI C'ACGA_G
Cuts at: 2976 4390 5606 6618 2976
Size: 1414 1216 1012 3904
Fragments arranged by size:
3904 1414 1216 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3025 3720 4312 794
Size: 2231 695 592 4028
Fragments arranged by size:
4028 2231 695 592
BstBI TT'CG_AA
Cuts at: 6350 6350
Size: 7546
BstEII G'GTnAC_C
Cuts at: 1184 4708 1184
Size: 3524 4022
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 7546
BstYI r'GATC_y
Cuts at: 1972 2172 2352 3101 3855 4683 4876 5243 5628
Size: 200 180 749 754 828 193 367 385
Cuts at: 5628 5645 7097 7183 7195 7541 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 828 754 749 385 367 346
200 193 180 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 513
Size: 552 939 132 5923
Fragments arranged by size:
5923 939 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2230 2293 3594 4645 487
Size: 683 56 1004 63 1301 1051 3388
Fragments arranged by size:
3388 1301 1051 1004 683 63 56
BtsI GCAGTG_nn'
Cuts at: 2016 2055 4778 5062 5856 5876 2016
Size: 39 2723 284 794 20 3686
Fragments arranged by size:
3686 2723 794 284 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7511
DraI TTT'AAA
Cuts at: 4850 4850
Size: 7546
DraIII CAC_nnn'GTG
Cuts at: 3928 3928
Size: 7546
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6553 809
Size: 507 5237 1802
Fragments arranged by size:
5237 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 2657 3072
Size: 54 1425 50 275 18 657 170 415
Cuts at: 3072 3610 4106 4825 4993 5882 7511 8
Size: 538 496 719 168 889 1629 43
Fragments arranged by size:
1629 1425 889 719 657 538 496 415
275 170 168 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2657 4993 1537
Size: 275 845 2336 4090
Fragments arranged by size:
4090 2336 845 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 2444 3983 5476 1353
Size: 785 306 1539 1493 3423
Fragments arranged by size:
3423 1539 1493 785 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3206 3831 6133 6507 6653 387
Size: 1064 138 1617 625 2302 374 146 1280
Fragments arranged by size:
2302 1617 1280 1064 625 374 146 138
EcoICRI GAG'CTC
Cuts at: 2115 2454 3042 4479 2115
Size: 339 588 1437 5182
Fragments arranged by size:
5182 1437 588 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2624 2804 2864 2978 3082 3524 3763
Size: 393 992 180 60 114 104 442 239
Cuts at: 3763 4007 4127 4268 4388 4507 4516 4684 4825
Size: 244 120 141 120 119 9 168 141
Cuts at: 4825 4831 4853 5609 6206 6993 7261 1239
Size: 6 22 756 597 787 268 1524
Fragments arranged by size:
1524 992 787 756 597 442 393 268
244 239 180 168 141 141 120 120
119 114 104 60 22 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3494 3494
Size: 7546
EcoO109I rG'GnC_Cy
Cuts at: 2505 2650 2750 3389 3478 3499 4092 4093 4100
Size: 145 100 639 89 21 593 1 7
Cuts at: 4100 4101 2505
Size: 1 5950
Fragments arranged by size:
5950 639 593 145 100 89 21 7
1 1
EcoRI G'AATT_C
Cuts at: 4855 5233 4855
Size: 378 7168
FspI TGC'GCA
Cuts at: 6052 6052
Size: 7546
HaeII r_GCGC'y
Cuts at: 1678 2565 6693 1678
Size: 887 4128 2531
Fragments arranged by size:
4128 2531 887
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2332 2364 2534 2566 2664
Size: 32 135 32 760 32 170 32 98
Cuts at: 2664 2696 3177 3209 4397 4429 6187 6219 6261
Size: 32 481 32 1188 32 1758 32 42
Cuts at: 6261 6293 1373
Size: 32 2626
Fragments arranged by size:
2626 1758 1188 760 481 170 135 98
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3400 4890 5136 669
Size: 988 281 1462 1490 246 3079
Fragments arranged by size:
3079 1490 1462 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2126 748
Size: 1378 6168
HpaI GTT'AAC
Cuts at: 5136 5136
Size: 7546
KasI G'GCGC_C
Cuts at: 2561 2561
Size: 7546
KpnI G_GTAC'C
Cuts at: 4871 4871
Size: 7546
MfeI C'AATT_G
Cuts at: 5145 5145
Size: 7546
MluI A'CGCG_T
Cuts at: 7422 7422
Size: 7546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2384 4401 6660 6844 1305
Size: 1079 2017 2259 184 2007
Fragments arranged by size:
2259 2017 2007 1079 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 3074 3612 4827 10
Size: 54 1425 343 657 585 538 1215 2729
Fragments arranged by size:
2729 1425 1215 657 585 538 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2534 3407 4745 5504 5863 6022
Size: 648 843 525 873 1338 759 359 159
Cuts at: 6022 518
Size: 2042
Fragments arranged by size:
2042 1338 873 843 759 648 525 359
159
MspA1I CmG'CkG
Cuts at: 1606 2231 2967 3442 3750 3938 4112 4321 4527
Size: 625 736 475 308 188 174 209 206
Cuts at: 4527 4691 4831 4962 5639 6787 7032 1606
Size: 164 140 131 677 1148 245 2120
Fragments arranged by size:
2120 1148 736 677 625 475 308 245
209 206 188 174 164 140 131
NaeI GCC'GGC
Cuts at: 2661 2661
Size: 7546
NarI GG'CG_CC
Cuts at: 2562 2562
Size: 7546
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6055
Fragments arranged by size:
6055 939 552
NdeI CA'TA_TG
Cuts at: 387 2569 387
Size: 2182 5364
NgoMIV G'CCGG_C
Cuts at: 2659 2659
Size: 7546
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7546
NotI GC'GGCC_GC
Cuts at: 4993 4993
Size: 7546
NspI r_CATG'y
Cuts at: 1132 1229 3023 3336 3772 4904 6449 1132
Size: 97 1794 313 436 1132 1545 2229
Fragments arranged by size:
2229 1794 1545 1132 436 313 97
PasI CC'CwG_GG
Cuts at: 1348 3433 4097 1348
Size: 2085 664 4797
Fragments arranged by size:
4797 2085 664
PciI A'CATG_T
Cuts at: 6445 6445
Size: 7546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2618 3081 3502 1108
Size: 1510 463 421 5152
Fragments arranged by size:
5152 1510 463 421
PfoI T'CCnGG_A
Cuts at: 4299 4493 4629 4299
Size: 194 136 7216
Fragments arranged by size:
7216 194 136
PmeI GTTT'AAAC
Cuts at: 4850 4850
Size: 7546
PmlI CAC'GTG
Cuts at: 2719 2719
Size: 7546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5615 5647 7128 7160 5615
Size: 32 1481 32 6001
Fragments arranged by size:
6001 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2505 2505
Size: 7546
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7546
PsiI TTA'TAA
Cuts at: 5116 5116
Size: 7546
PspOMI G'GGCC_C
Cuts at: 3477 4092 4100 3477
Size: 615 8 6923
Fragments arranged by size:
6923 615 8
PstI C_TGCA'G
Cuts at: 830 3937 4317 4696 4773 4898 830
Size: 3107 380 379 77 125 3478
Fragments arranged by size:
3478 3107 380 379 125 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7546
PvuII CAG'CTG
Cuts at: 2231 2967 3442 3750 4112 4321 4527 4691 4831
Size: 736 475 308 362 209 206 164 140
Cuts at: 4831 2231
Size: 4946
Fragments arranged by size:
4946 736 475 362 308 209 206 164
140
SacI G_AGCT'C
Cuts at: 2117 2456 3044 4481 2117
Size: 339 588 1437 5182
Fragments arranged by size:
5182 1437 588 339
SalI G'TCGA_C
Cuts at: 1936 4888 1936
Size: 2952 4594
SapI GCTCTTCn'nnn_
Cuts at: 2138 2444 3983 2138
Size: 306 1539 5701
Fragments arranged by size:
5701 1539 306
SbfI CC_TGCA'GG
Cuts at: 4898 4898
Size: 7546
ScaI AGT'ACT
Cuts at: 5794 7506 5794
Size: 1712 5834
SfcI C'TryA_G
Cuts at: 826 1045 2376 2782 3933 3968 4313 4692 4769
Size: 219 1331 406 1151 35 345 379 77
Cuts at: 4769 4894 6029 6710 6901 826
Size: 125 1135 681 191 1471
Fragments arranged by size:
1471 1331 1151 1135 681 406 379 345
219 191 125 77 35
SfoI GGC'GCC
Cuts at: 2563 2563
Size: 7546
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7546
SmaI CCC'GGG
Cuts at: 1872 3107 4547 4873 1872
Size: 1235 1440 326 4545
Fragments arranged by size:
4545 1440 1235 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 3161 3821 5650 6551 6813
Size: 197 926 186 1032 660 1829 901 262
Cuts at: 6813 820
Size: 1553
Fragments arranged by size:
1829 1553 1032 926 901 660 262 197
186
SnaBI TAC'GTA
Cuts at: 493 5337 493
Size: 4844 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7546
SphI G_CATG'C
Cuts at: 1132 3023 4904 1132
Size: 1891 1881 3774
Fragments arranged by size:
3774 1891 1881
SspI AAT'ATT
Cuts at: 5 52 5470 5
Size: 47 5418 2081
Fragments arranged by size:
5418 2081 47
StuI AGG'CCT
Cuts at: 1517 2616 4451 1517
Size: 1099 1835 4612
Fragments arranged by size:
4612 1835 1099
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2193 2205 2484 2617 3392
Size: 552 939 75 114 12 279 133 775
Cuts at: 3392 3729 3995 4088 4985 513
Size: 337 266 93 897 3074
Fragments arranged by size:
3074 939 897 775 552 337 279 266
133 114 93 75 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3367 5573 5590 5743 5928 6373 325
Size: 1255 1787 2206 17 153 185 445 1498
Fragments arranged by size:
2206 1787 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 3483 5792
Size: 274 80 33 51 1443 348 1158 2309
Cuts at: 5792 7504 96
Size: 1712 138
Fragments arranged by size:
2309 1712 1443 1158 348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3939 3971 7506 7538 3939
Size: 32 3535 32 3947
Fragments arranged by size:
3947 3535 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7546
XbaI T'CTAG_A
Cuts at: 4882 4882
Size: 7546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4531 2011
Size: 2520 5026
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7546
XmaI C'CCGG_G
Cuts at: 1870 3105 4545 4871 1870
Size: 1235 1440 326 4545
Fragments arranged by size:
4545 1440 1235 326
XmnI GAAnn'nnTTC
Cuts at: 3309 5675 3309
Size: 2366 5180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3550 5354 276
Size: 53 83 186 936 2016 1804 2468
Fragments arranged by size:
2468 2016 1804 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI