Restriction map for
pFN21AE1467
(Circular) (Six-base) MAPSORT of: pFN21AE1467.seq Check: 8789 from: 1 to: 6415
pFN21AE1467 6415 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2514 3326 3642 4478 5862 6130 1632
Size: 882 812 316 836 1384 268 1917
Fragments arranged by size:
1917 1384 882 836 812 316 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 5314 6291 1203
Size: 4111 977 1327
Fragments arranged by size:
4111 1327 977
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 2900 1166
Size: 1734 4681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6415
AlwNI CAG_nnn'CTG
Cuts at: 4158 5730 4158
Size: 1572 4843
ApaI G_GGCC'C
Cuts at: 2170 3174 2170
Size: 1004 5411
ApaLI G'TGCA_C
Cuts at: 3482 4472 5628 3482
Size: 990 1156 4269
Fragments arranged by size:
4269 1156 990
ApoI r'AATT_y
Cuts at: 1493 2185 3560 3724 3950 4102 6211 1493
Size: 692 1375 164 226 152 2109 1697
Fragments arranged by size:
2109 1697 1375 692 226 164 152
AseI AT'TA_AT
Cuts at: 160 1032 2567 4969 160
Size: 872 1535 2402 1606
Fragments arranged by size:
2402 1606 1535 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 2391 3678 3740 1870
Size: 73 448 1287 62 4545
Fragments arranged by size:
4545 1287 448 73 62
BamHI G'GATC_C
Cuts at: 3745 4112 3745
Size: 367 6048
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3015 3736 5191 618
Size: 325 217 627 1228 721 1455 1842
Fragments arranged by size:
1842 1455 1228 721 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 2170 3174 3734 1391
Size: 643 136 1004 560 4072
Fragments arranged by size:
4072 1004 643 560 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2065 2284 3054 928
Size: 971 166 219 770 4289
Fragments arranged by size:
4289 971 770 219 166
BbvCI CC'TCA_GC
Cuts at: 3425 3425
Size: 6415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3071 4307 5517 3071
Size: 1236 1210 3969
Fragments arranged by size:
3969 1236 1210
BclI T'GATC_A
Cuts at: 1548 3507 1548
Size: 1959 4456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5026 136
Size: 107 122 71 631 605 3354 1525
Fragments arranged by size:
3354 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3318 3370 6410 3318
Size: 52 3040 3323
Fragments arranged by size:
3323 3040 52
BlpI GC'TnA_GC
Cuts at: 3832 3832
Size: 6415
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2993 3174 3486 4476 5632 1790
Size: 380 823 181 312 990 1156 2573
Fragments arranged by size:
2573 1156 990 823 380 312 181
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2023 3559 4146
Size: 416 53 83 399 24 606 1536 587
Cuts at: 4146 5104 442
Size: 958 1753
Fragments arranged by size:
1753 1536 958 606 587 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 3626 2050
Size: 1576 4839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 3029 3407 5075 1239
Size: 1320 470 378 1668 2579
Fragments arranged by size:
2579 1668 1320 470 378
Bpu10I CC'TnA_GC
Cuts at: 3425 3425
Size: 6415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3248 4540 5405 5703 3248
Size: 1292 865 298 3960
Fragments arranged by size:
3960 1292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5078 882
Size: 617 3579 2219
Fragments arranged by size:
3579 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2690 4206 493
Size: 711 1486 1516 2702
Fragments arranged by size:
2702 1516 1486 711
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4222 4604 5239 275
Size: 53 83 186 936 2689 382 635 1451
Fragments arranged by size:
2689 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2595 4848 5246 5520 5667 6078 2595
Size: 2253 398 274 147 411 2932
Fragments arranged by size:
2932 2253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6190
Fragments arranged by size:
6190 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3509 1186
Size: 2323 4092
BseYI C'CCAG_C
Cuts at: 2059 3450 5618 6114 2059
Size: 1391 2168 496 2360
Fragments arranged by size:
2360 2168 1391 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5842
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 4626 5654 1540
Size: 275 186 1864 761 1028 2301
Fragments arranged by size:
2301 1864 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 4476 4561 5632 3486
Size: 990 85 1071 4269
Fragments arranged by size:
4269 1071 990 85
BsmI GAATG_Cn'
Cuts at: 2392 2777 3926 4019 2392
Size: 385 1149 93 4788
Fragments arranged by size:
4788 1149 385 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2170 2993 3174 3486 3734 4476
Size: 399 244 136 823 181 312 248 742
Cuts at: 4476 4561 5632 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 823 742 399 312 248 244
181 136 85
BspEI T'CCGG_A
Cuts at: 2595 6078 2595
Size: 3483 2932
BspHI T'CATG_A
Cuts at: 4304 6034 4304
Size: 1730 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3770 844
Size: 2926 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3230 4302 1394
Size: 429 1407 1072 3507
Fragments arranged by size:
3507 1407 1072 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2352 4910 5084 65
Size: 1183 1104 2558 174 1396
Fragments arranged by size:
2558 1396 1183 1104 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3416 5059 5246 1700
Size: 110 1606 1643 187 2869
Fragments arranged by size:
2869 1643 1606 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 4475 5487 4475
Size: 1012 5403
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6415
BstBI TT'CG_AA
Cuts at: 2275 5219 2275
Size: 2944 3471
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 3318 3370 3745 4112 4497 4514 5966 6052
Size: 1346 52 375 367 385 17 1452 86
Cuts at: 6052 6064 6410 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1346 385 375 367 346 86
52 17 12
Bsu36I CC'TnA_GG
Cuts at: 2240 3042 2240
Size: 802 5613
BtgI C'CryG_G
Cuts at: 513 1065 2004 2668 513
Size: 552 939 664 4260
Fragments arranged by size:
4260 939 664 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5676
Fragments arranged by size:
5676 683 56
BtsI GCAGTG_nn'
Cuts at: 3180 3931 4725 4745 3180
Size: 751 794 20 4850
Fragments arranged by size:
4850 794 751 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3091 3122 3126 3157 529
Size: 35 2527 31 4 31 3787
Fragments arranged by size:
3787 2527 35 31 31 4
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DraIII CAC_nnn'GTG
Cuts at: 2556 2556
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5422 809
Size: 507 4106 1802
Fragments arranged by size:
4106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 3862 4751
Size: 54 1425 50 275 18 330 1702 889
Cuts at: 4751 6380 8
Size: 1629 43
Fragments arranged by size:
1702 1629 1425 889 330 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4345 1353
Size: 2992 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3025 5002 5376 5522 387
Size: 1064 138 1436 1977 374 146 1280
Fragments arranged by size:
1977 1436 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2514 2559 3029 3326 3407 3642 4478
Size: 393 882 45 470 297 81 235 836
Cuts at: 4478 5075 5862 6130 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 882 836 787 597 470 393 297
268 235 81 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2239 2496 2239
Size: 257 6158
EcoO109I rG'GnC_Cy
Cuts at: 2166 2167 2828 3171 2166
Size: 1 661 343 5410
Fragments arranged by size:
5410 661 343 1
EcoRI G'AATT_C
Cuts at: 3560 3724 4102 3560
Size: 164 378 5873
Fragments arranged by size:
5873 378 164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3340 3727 3340
Size: 387 6028
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 5562 1678
Size: 3884 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2532 2564 2812 2844 5056
Size: 32 135 32 960 32 248 32 2212
Cuts at: 5056 5088 5130 5162 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2212 960 248 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3581 3759 4005 669
Size: 988 281 1643 178 246 3079
Fragments arranged by size:
3079 1643 988 281 246 178
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6415
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 3570 4014 3570
Size: 444 5971
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3684 5529 5713 1305
Size: 2379 1845 184 2007
Fragments arranged by size:
2379 2007 1845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 10
Size: 54 1425 343 330 4263
Fragments arranged by size:
4263 1425 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2900 3489 4373 4732 4891 518
Size: 648 1734 589 884 359 159 2042
Fragments arranged by size:
2042 1734 884 648 589 359 159
MspA1I CmG'CkG
Cuts at: 1606 3831 4508 5656 5901 1606
Size: 2225 677 1148 245 2120
Fragments arranged by size:
2225 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3418 3418
Size: 6415
NcoI C'CATG_G
Cuts at: 513 1065 2004 2668 513
Size: 552 939 664 4260
Fragments arranged by size:
4260 939 664 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NgoMIV G'CCGG_C
Cuts at: 3416 3416
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 2200 3353 3773 5318 1132
Size: 97 971 1153 420 1545 2229
Fragments arranged by size:
2229 1545 1153 971 420 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6415
PciI A'CATG_T
Cuts at: 5314 5314
Size: 6415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6415
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 4484 4516 5997 6029 3083
Size: 32 1369 32 1481 32 3469
Fragments arranged by size:
3469 1481 1369 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2828 2828
Size: 6415
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 3985 3985
Size: 6415
PspOMI G'GGCC_C
Cuts at: 2166 3170 2166
Size: 1004 5411
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2670 2702 2670
Size: 32 6383
PstI C_TGCA'G
Cuts at: 830 2176 3767 830
Size: 1346 1591 3478
Fragments arranged by size:
3478 1591 1346
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SanDI GG'GwC_CC
Cuts at: 2828 2828
Size: 6415
SbfI CC_TGCA'GG
Cuts at: 3767 3767
Size: 6415
ScaI AGT'ACT
Cuts at: 4663 6375 4663
Size: 1712 4703
SfcI C'TryA_G
Cuts at: 826 1045 2172 2933 3763 4898 5579 5770 826
Size: 219 1127 761 830 1135 681 191 1471
Fragments arranged by size:
1471 1135 1127 830 761 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3263 4519 5420 5682 820
Size: 197 926 1320 1256 901 262 1553
Fragments arranged by size:
1553 1320 1256 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 2200 3773 1132
Size: 1068 1573 3774
Fragments arranged by size:
3774 1573 1068
SspI AAT'ATT
Cuts at: 5 52 3284 4339 5
Size: 47 3232 1055 2081
Fragments arranged by size:
3232 2081 1055 47
StuI AGG'CCT
Cuts at: 1517 2502 3516 1517
Size: 985 1014 4416
Fragments arranged by size:
4416 1014 985
StyI C'CwwG_G
Cuts at: 513 1065 2004 2668 2898 3627 3854 513
Size: 552 939 664 230 729 227 3074
Fragments arranged by size:
3074 939 729 664 552 230 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4442 4459 4612 4797 5242 325
Size: 1255 2862 17 153 185 445 1498
Fragments arranged by size:
2862 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4661 6373 96
Size: 274 80 33 51 1443 2684 1712 138
Fragments arranged by size:
2684 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6375 6407 6375
Size: 32 6383
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6415
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6415
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 2072 2951 4544 2072
Size: 879 1593 3943
Fragments arranged by size:
3943 1593 879
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI