Restriction map for
pFN21AE1466
(Circular) (Six-base) MAPSORT of: pFN21AE1466.seq Check: 6632 from: 1 to: 6700
pFN21AE1466 6700 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3668 3668
Size: 6700
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4510 278
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4043 1937
Size: 2106 4594
Acc65I G'GTAC_C
Cuts at: 4021 4021
Size: 6700
AclI AA'CG_TT
Cuts at: 1563 4827 5200 1563
Size: 3264 373 3063
Fragments arranged by size:
3264 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2604 4763 6147 6415 1632
Size: 972 2159 1384 268 1917
Fragments arranged by size:
2159 1917 1384 972 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6503
AflIII A'CryG_T
Cuts at: 1203 3588 5599 6576 1203
Size: 2385 2011 977 1327
Fragments arranged by size:
2385 2011 1327 977
AgeI A'CCGG_T
Cuts at: 3277 5531 3277
Size: 2254 4446
AhdI GACnn_n'nnGTC
Cuts at: 5429 5429
Size: 6700
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6700
AlwNI CAG_nnn'CTG
Cuts at: 4443 6015 4443
Size: 1572 5128
ApaLI G'TGCA_C
Cuts at: 4757 5913 4757
Size: 1156 5544
ApoI r'AATT_y
Cuts at: 1493 4009 4235 4387 6496 1493
Size: 2516 226 152 2109 1697
Fragments arranged by size:
2516 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5254 160
Size: 872 4222 1606
Fragments arranged by size:
4222 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
AvaI C'yCGr_G
Cuts at: 1870 1943 3259 4025 1870
Size: 73 1316 766 4545
Fragments arranged by size:
4545 1316 766 73
BamHI G'GATC_C
Cuts at: 4030 4397 4030
Size: 367 6333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2304 2310 2588 3062 3712
Size: 325 217 627 517 6 278 474 650
Cuts at: 3712 4021 5476 618
Size: 309 1455 1842
Fragments arranged by size:
1842 1455 650 627 517 474 325 309
278 217 6
BanII G_rGCy'C
Cuts at: 1391 4019 1391
Size: 2628 4072
BbeI G_GCGC'C
Cuts at: 2308 2308
Size: 6700
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 2438 928
Size: 971 351 188 5190
Fragments arranged by size:
5190 971 351 188
BbvCI CC'TCA_GC
Cuts at: 3706 3860 3706
Size: 154 6546
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4891 4925 1903
Size: 34 2954 34 3678
Fragments arranged by size:
3678 2954 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4592 5802 4592
Size: 1210 5490
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6700
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5311 136
Size: 107 122 71 631 605 3639 1525
Fragments arranged by size:
3639 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3361 6695 3361
Size: 3334 3366
BlpI GC'TnA_GC
Cuts at: 4117 4117
Size: 6700
Bme1580I G_kGCm'C
Cuts at: 1790 2238 3717 4761 5917 1790
Size: 448 1479 1044 1156 2573
Fragments arranged by size:
2573 1479 1156 1044 448
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2232 3478 4431
Size: 416 53 83 399 24 815 1246 953
Cuts at: 4431 5389 442
Size: 958 1753
Fragments arranged by size:
1753 1246 958 953 815 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6700
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3492 3492
Size: 6700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2196 2751 5360 1239
Size: 957 555 2609 2579
Fragments arranged by size:
2609 2579 957 555
Bpu10I CC'TnA_GC
Cuts at: 3706 3839 3860 3706
Size: 133 21 6546
Fragments arranged by size:
6546 133 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2551 4825 5690 5988 2375
Size: 176 2274 865 298 3087
Fragments arranged by size:
3087 2274 865 298 176
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3268 3869 5363 882
Size: 617 1769 601 1494 2219
Fragments arranged by size:
2219 1769 1494 617 601
BsaAI yAC'GTr
Cuts at: 493 1204 4491 493
Size: 711 3287 2702
Fragments arranged by size:
3287 2702 711
BsaBI GATnn'nnATC
Cuts at: 6694 6694
Size: 6700
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2305 4507 4889 5524
Size: 53 83 186 936 772 2202 382 635
Cuts at: 5524 275
Size: 1451
Fragments arranged by size:
2202 1451 936 772 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2986 3277 5133 5531 5805 5952 6363 2986
Size: 291 1856 398 274 147 411 3323
Fragments arranged by size:
3323 1856 411 398 291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2749 2779 2868 2898 1178
Size: 30 165 30 1346 30 89 30 4980
Fragments arranged by size:
4980 1346 165 89 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3594 3848 3877 1186
Size: 2408 254 29 4009
Fragments arranged by size:
4009 2408 254 29
BseYI C'CCAG_C
Cuts at: 2040 5903 6399 2040
Size: 3863 496 2341
Fragments arranged by size:
3863 2341 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 1163
Size: 573 474 5653
Fragments arranged by size:
5653 573 474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3828 4150 4911 5939 1540
Size: 275 186 1827 322 761 1028 2301
Fragments arranged by size:
2301 1827 1028 761 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 4761 4846 5917 4761
Size: 85 1071 5544
Fragments arranged by size:
5544 1071 85
BsmI GAATG_Cn'
Cuts at: 2321 4211 4304 2321
Size: 1890 93 4717
Fragments arranged by size:
4717 1890 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3774 3774
Size: 6700
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 3717 4019 4761 4846 5917 1391
Size: 399 448 1479 302 742 85 1071 2174
Fragments arranged by size:
2174 1479 1071 742 448 399 302 85
BspEI T'CCGG_A
Cuts at: 6363 6363
Size: 6700
BspHI T'CATG_A
Cuts at: 4589 6319 4589
Size: 1730 4970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2340 2758 3668 4055 844
Size: 1496 418 910 387 3489
Fragments arranged by size:
3489 1496 910 418 387
BsrBI CCG'CTC
Cuts at: 1394 1823 3038 4587 1394
Size: 429 1215 1549 3507
Fragments arranged by size:
3507 1549 1215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3385 5195 5369 65
Size: 1183 2137 1810 174 1396
Fragments arranged by size:
2137 1810 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2307 2719 3277 3329 5344 5531 1700
Size: 110 497 412 558 52 2015 187 2869
Fragments arranged by size:
2869 2015 558 497 412 187 110 52
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6700
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6700
BssSI C'ACGA_G
Cuts at: 4760 5772 4760
Size: 1012 5688
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2233 794
Size: 1439 5261
BstBI TT'CG_AA
Cuts at: 5504 5504
Size: 6700
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6700
BstYI r'GATC_y
Cuts at: 1972 3361 4030 4397 4782 4799 6251 6337 6349
Size: 1389 669 367 385 17 1452 86 12
Cuts at: 6349 6695 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1389 669 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3408 3669 3828 513
Size: 552 939 1404 261 159 3385
Fragments arranged by size:
3385 1404 939 552 261 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5961
Fragments arranged by size:
5961 683 56
BtsI GCAGTG_nn'
Cuts at: 3251 4216 5010 5030 3251
Size: 965 794 20 4921
Fragments arranged by size:
4921 965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6700
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6665
DraI TTT'AAA
Cuts at: 4004 4004
Size: 6700
DraIII CAC_nnn'GTG
Cuts at: 2233 2233
Size: 6700
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3282 5707 809
Size: 507 1966 2425 1802
Fragments arranged by size:
2425 1966 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3215 3327 3810
Size: 54 1425 50 275 18 1385 112 483
Cuts at: 3810 3825 4147 5036 6665 8
Size: 15 322 889 1629 43
Fragments arranged by size:
1629 1425 1385 889 483 322 275 112
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3825 4147 1537
Size: 275 2013 322 4090
Fragments arranged by size:
4090 2013 322 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3537 3927 4630 1353
Size: 2184 390 703 3423
Fragments arranged by size:
3423 2184 703 390
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5287 5661 5807 387
Size: 1064 138 3698 374 146 1280
Fragments arranged by size:
3698 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2604 2751 4763 5360 6147 6415
Size: 393 564 408 147 2012 597 787 268
Cuts at: 6415 1239
Size: 1524
Fragments arranged by size:
2012 1524 787 597 564 408 393 268
147
EcoRI G'AATT_C
Cuts at: 4009 4387 4009
Size: 378 6322
EcoRV GAT'ATC
Cuts at: 3520 3520
Size: 6700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3340 3340
Size: 6700
FspI TGC'GCA
Cuts at: 3573 5206 3573
Size: 1633 5067
HaeII r_GCGC'y
Cuts at: 1678 2308 5847 1678
Size: 630 3539 2531
Fragments arranged by size:
3539 2531 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 2868 2900 3217
Size: 32 135 32 926 32 338 32 317
Cuts at: 3217 3249 5341 5373 5415 5447 1373
Size: 32 2092 32 42 32 2626
Fragments arranged by size:
2626 2092 926 338 317 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4044 4290 669
Size: 988 281 2106 246 3079
Fragments arranged by size:
3079 2106 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6700
HpaI GTT'AAC
Cuts at: 4290 4290
Size: 6700
KasI G'GCGC_C
Cuts at: 2304 2304
Size: 6700
KpnI G_GTAC'C
Cuts at: 4025 4025
Size: 6700
MfeI C'AATT_G
Cuts at: 2694 4299 2694
Size: 1605 5095
MluI A'CGCG_T
Cuts at: 3588 6576 3588
Size: 2988 3712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 2475 3464 3906 5814 5998 1305
Size: 1155 15 989 442 1908 184 2007
Fragments arranged by size:
2007 1908 1155 989 442 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4878
Fragments arranged by size:
4878 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2381 4658 5017 5176 518
Size: 648 883 332 2277 359 159 2042
Fragments arranged by size:
2277 2042 883 648 359 332 159
MspA1I CmG'CkG
Cuts at: 1606 3289 3710 3746 4116 4793 5941 6186 1606
Size: 1683 421 36 370 677 1148 245 2120
Fragments arranged by size:
2120 1683 1148 677 421 370 245 36
NaeI GCC'GGC
Cuts at: 3331 3331
Size: 6700
NarI GG'CG_CC
Cuts at: 2305 2305
Size: 6700
NcoI C'CATG_G
Cuts at: 513 1065 2004 3669 513
Size: 552 939 1665 3544
Fragments arranged by size:
3544 1665 939 552
NdeI CA'TA_TG
Cuts at: 387 2836 387
Size: 2449 4251
NgoMIV G'CCGG_C
Cuts at: 3329 3329
Size: 6700
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6700
NotI GC'GGCC_GC
Cuts at: 4147 4147
Size: 6700
NspI r_CATG'y
Cuts at: 1132 1229 4058 5603 1132
Size: 97 2829 1545 2229
Fragments arranged by size:
2829 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6700
PciI A'CATG_T
Cuts at: 5599 5599
Size: 6700
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6700
PfoI T'CCnGG_A
Cuts at: 3351 3351
Size: 6700
PmeI GTTT'AAAC
Cuts at: 4004 4004
Size: 6700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4769 4801 6282 6314 4769
Size: 32 1481 32 5155
Fragments arranged by size:
5155 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2689 3848 1613
Size: 1076 1159 4465
Fragments arranged by size:
4465 1159 1076
PsiI TTA'TAA
Cuts at: 4270 4270
Size: 6700
PstI C_TGCA'G
Cuts at: 830 2614 4052 830
Size: 1784 1438 3478
Fragments arranged by size:
3478 1784 1438
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6700
PvuII CAG'CTG
Cuts at: 3746 3746
Size: 6700
RsrII CG'GwC_CG
Cuts at: 3370 3370
Size: 6700
SalI G'TCGA_C
Cuts at: 1936 4042 1936
Size: 2106 4594
SbfI CC_TGCA'GG
Cuts at: 4052 4052
Size: 6700
ScaI AGT'ACT
Cuts at: 4948 6660 4948
Size: 1712 4988
SfcI C'TryA_G
Cuts at: 826 1045 2610 2757 3462 4048 5183 5864 6055
Size: 219 1565 147 705 586 1135 681 191
Cuts at: 6055 826
Size: 1471
Fragments arranged by size:
1565 1471 1135 705 681 586 219 191
147
SfoI GGC'GCC
Cuts at: 2306 2306
Size: 6700
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6700
SgrAI Cr'CCGG_yG
Cuts at: 1700 2307 1700
Size: 607 6093
SmaI CCC'GGG
Cuts at: 1872 4027 1872
Size: 2155 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 2566 4804 5705 5967 820
Size: 197 926 411 212 2238 901 262 1553
Fragments arranged by size:
2238 1553 926 901 411 262 212 197
SnaBI TAC'GTA
Cuts at: 493 4491 493
Size: 3998 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6700
SphI G_CATG'C
Cuts at: 1132 4058 1132
Size: 2926 3774
SspI AAT'ATT
Cuts at: 5 52 2829 4624 5
Size: 47 2777 1795 2081
Fragments arranged by size:
2777 2081 1795 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6700
StyI C'CwwG_G
Cuts at: 513 1065 2004 2687 3669 4139 513
Size: 552 939 683 982 470 3074
Fragments arranged by size:
3074 982 939 683 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 4727 4744 4897 5082 5527 325
Size: 1255 480 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4946 6658 96
Size: 274 80 33 51 1443 2969 1712 138
Fragments arranged by size:
2969 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2393 2425 6660 6692 2393
Size: 32 4235 32 2401
Fragments arranged by size:
4235 2401 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6700
XbaI T'CTAG_A
Cuts at: 4036 4036
Size: 6700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 2599
Size: 6700
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6700
XmaI C'CCGG_G
Cuts at: 1870 4025 1870
Size: 2155 4545
XmnI GAAnn'nnTTC
Cuts at: 2418 3117 4829 2418
Size: 699 1712 4289
Fragments arranged by size:
4289 1712 699
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4508 276
Size: 53 83 186 936 2974 2468
Fragments arranged by size:
2974 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI