(Circular) (Six-base) MAPSORT of: pFN21AE1462.seq Check: 120 from: 1 to: 5413 pFN21AE1462 5413 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 2712 3540 3913 1563 Size: 1149 828 373 3063 Fragments arranged by size: 3063 1149 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 3476 4860 5128 1632 Size: 545 1299 1384 268 1917 Fragments arranged by size: 1917 1384 1299 545 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 4312 5289 1203 Size: 3109 977 1327 Fragments arranged by size: 3109 1327 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 2632 4142 2632 Size: 1510 3903 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 2006 Size: 32 5381 AlwNI CAG_nnn'CTG Cuts at: 2155 3156 4728 2155 Size: 1001 1572 2840 Fragments arranged by size: 2840 1572 1001 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2073 2722 2948 3100 5209 1493 Size: 580 649 226 152 2109 1697 Fragments arranged by size: 2109 1697 649 580 226 152 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2268 2738 1870 Size: 73 325 470 4545 Fragments arranged by size: 4545 470 325 73 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2113 2306 2734 4189 Size: 325 217 627 221 105 193 428 1455 Cuts at: 4189 618 Size: 1842 Fragments arranged by size: 1842 1455 627 428 325 221 217 193 105 BanII G_rGCy'C Cuts at: 1391 2732 1391 Size: 1341 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2245 928 Size: 971 346 4096 Fragments arranged by size: 4096 971 346 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3604 3638 1903 Size: 34 1667 34 3678 Fragments arranged by size: 3678 1667 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4515 3305 Size: 1210 4203 BclI T'GATC_A Cuts at: 1548 2652 1548 Size: 1104 4309 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4024 136 Size: 107 122 71 631 605 2352 1525 Fragments arranged by size: 2352 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2317 5408 2317 Size: 3091 2322 BlpI GC'TnA_GC Cuts at: 2352 2830 2352 Size: 478 4935 Bme1580I G_kGCm'C Cuts at: 1790 3474 4630 1790 Size: 1684 1156 2573 Fragments arranged by size: 2573 1684 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 4102 442 Size: 416 53 83 399 24 1727 958 1753 Fragments arranged by size: 1753 1727 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4073 1239 Size: 2834 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3538 4403 4701 3538 Size: 865 298 4250 Fragments arranged by size: 4250 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2390 4076 882 Size: 617 891 1686 2219 Fragments arranged by size: 2219 1686 891 617 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3220 3602 4237 275 Size: 53 83 186 936 1687 382 635 1451 Fragments arranged by size: 1687 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2011 3846 4244 4518 4665 5076 2011 Size: 1835 398 274 147 411 2348 Fragments arranged by size: 2348 1835 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5188 Fragments arranged by size: 5188 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2373 2399 2408 1186 Size: 1187 26 9 4191 Fragments arranged by size: 4191 1187 26 9 BseYI C'CCAG_C Cuts at: 4616 5112 4616 Size: 496 4917 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4840 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2365 3474 3559 4630 2365 Size: 1109 85 1071 3148 Fragments arranged by size: 3148 1109 1071 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2365 2732 3474 3559 4630 1391 Size: 399 575 367 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 399 367 85 BspEI T'CCGG_A Cuts at: 5076 5076 Size: 5413 BspHI T'CATG_A Cuts at: 3302 5032 3302 Size: 1730 3683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 844 Size: 1924 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3300 1394 Size: 429 1477 3507 Fragments arranged by size: 3507 1477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2605 3908 4082 65 Size: 1183 1357 1303 174 1396 Fragments arranged by size: 1396 1357 1303 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4057 4244 1700 Size: 110 2247 187 2869 Fragments arranged by size: 2869 2247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5413 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstXI CCAn_nnnn'nTGG Cuts at: 2407 2572 2407 Size: 165 5248 BstYI r'GATC_y Cuts at: 1972 2317 2743 3110 3495 3512 4964 5050 5062 Size: 345 426 367 385 17 1452 86 12 Cuts at: 5062 5408 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 426 385 367 346 345 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3922 Fragments arranged by size: 3922 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3723 3743 2929 Size: 794 20 4599 Fragments arranged by size: 4599 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2702 2860 3749 Size: 54 1425 50 275 18 872 158 889 Cuts at: 3749 5378 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 872 275 158 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 3343 1353 Size: 987 1003 3423 Fragments arranged by size: 3423 1003 987 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 3476 4073 4860 5128 1239 Size: 393 545 1299 597 787 268 1524 Fragments arranged by size: 1524 1299 787 597 545 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2177 Size: 5413 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 4560 1678 Size: 2882 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2232 2264 2618 2650 4054 Size: 32 135 32 660 32 354 32 1404 Cuts at: 4054 4086 4128 4160 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1404 660 354 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2205 2757 3003 669 Size: 988 281 267 552 246 3079 Fragments arranged by size: 3079 988 552 281 267 246 HindIII A'AGCT_T Cuts at: 748 2120 748 Size: 1372 4041 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3591 Fragments arranged by size: 3591 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 3730 3889 518 Size: 648 2205 359 159 2042 Fragments arranged by size: 2205 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2829 3506 4654 4899 1606 Size: 1223 677 1148 245 2120 Fragments arranged by size: 2120 1223 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3922 Fragments arranged by size: 3922 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2771 4316 1132 Size: 97 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 2373 1108 Size: 1265 4148 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5413 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PstI C_TGCA'G Cuts at: 830 2765 830 Size: 1935 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SfcI C'TryA_G Cuts at: 826 1045 2761 3896 4577 4768 826 Size: 219 1716 1135 681 191 1471 Fragments arranged by size: 1716 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2268 3517 4418 4680 820 Size: 197 926 325 1249 901 262 1553 Fragments arranged by size: 1553 1249 926 901 325 262 197 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 2466 1517 Size: 949 4464 StyI C'CwwG_G Cuts at: 513 1065 2004 2372 2852 513 Size: 552 939 368 480 3074 Fragments arranged by size: 3074 939 552 480 368 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2250 2380 3440 3457 3610 3795 4240 Size: 1255 670 130 1060 17 153 185 445 Cuts at: 4240 325 Size: 1498 Fragments arranged by size: 1498 1255 1060 670 445 185 153 130 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3659 5371 96 Size: 274 80 33 51 1443 1682 1712 138 Fragments arranged by size: 1712 1682 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5373 5405 5373 Size: 32 5381 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 2268 1943 Size: 325 5088 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 2592 3542 2592 Size: 950 4463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI