Restriction map for
pFN21AE1461
(Circular) (Six-base) MAPSORT of: pFN21AE1461.seq Check: 7290 from: 1 to: 6181
pFN21AE1461 6181 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2157 2157
Size: 6181
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3524 1937
Size: 1587 4594
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 4244 5628 5896 1632
Size: 659 1953 1384 268 1917
Fragments arranged by size:
1953 1917 1384 659 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3488 1184
Size: 2304 3877
AlwNI CAG_nnn'CTG
Cuts at: 2314 2638 3019 3924 5496 2314
Size: 324 381 905 1572 2999
Fragments arranged by size:
2999 1572 905 381 324
ApaI G_GGCC'C
Cuts at: 2471 2741 3231 3303 2471
Size: 270 490 72 5349
Fragments arranged by size:
5349 490 270 72
ApaLI G'TGCA_C
Cuts at: 2857 4238 5394 2857
Size: 1381 1156 3644
Fragments arranged by size:
3644 1381 1156
ApoI r'AATT_y
Cuts at: 1493 3490 3716 3868 5977 1493
Size: 1997 226 152 2109 1697
Fragments arranged by size:
2109 1997 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 2280 2397 2673 3049 3132 3230 3313
Size: 73 337 117 276 376 83 98 83
Cuts at: 3313 3426 3506 1870
Size: 113 80 4545
Fragments arranged by size:
4545 376 337 276 117 113 98 83
83 80 73
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2514 2653 3437 3502 4957
Size: 325 217 627 727 139 784 65 1455
Cuts at: 4957 618
Size: 1842
Fragments arranged by size:
1842 1455 784 727 627 325 217 139
65
BanII G_rGCy'C
Cuts at: 1391 2108 2404 2471 2741 2915 3063 3231 3237
Size: 717 296 67 270 174 148 168 6
Cuts at: 3237 3303 3320 3421 3500 1391
Size: 66 17 101 79 4072
Fragments arranged by size:
4072 717 296 270 174 168 148 101
79 67 66 17 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3193 928
Size: 971 1294 3916
Fragments arranged by size:
3916 1294 971
BbvCI CC'TCA_GC
Cuts at: 2286 2286
Size: 6181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2135 2169 4372 4406 1903
Size: 34 198 34 2203 34 3678
Fragments arranged by size:
3678 2203 198 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3186 3241 4792
Size: 107 122 71 631 605 1514 55 1551
Cuts at: 4792 136
Size: 1525
Fragments arranged by size:
1551 1525 1514 631 605 122 107 71
55
BglII A'GATC_T
Cuts at: 6176 6176
Size: 6181
BlpI GC'TnA_GC
Cuts at: 2382 3598 2382
Size: 1216 4965
Bme1580I G_kGCm'C
Cuts at: 1790 2471 2741 2861 3231 3303 4242 5398 1790
Size: 681 270 120 370 72 939 1156 2573
Fragments arranged by size:
2573 1156 939 681 370 270 120 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3912 4870 442
Size: 416 53 83 399 24 2495 958 1753
Fragments arranged by size:
2495 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2422 2481 2923 4841 1239
Size: 1183 59 442 1918 2579
Fragments arranged by size:
2579 1918 1183 442 59
Bpu10I CC'TnA_GC
Cuts at: 2286 2286
Size: 6181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4306 5171 5469 4306
Size: 865 298 5018
Fragments arranged by size:
5018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3098 3279 4844 882
Size: 617 1599 181 1565 2219
Fragments arranged by size:
2219 1599 1565 617 181
BsaAI yAC'GTr
Cuts at: 493 1204 3972 493
Size: 711 2768 2702
Fragments arranged by size:
2768 2702 711
BsaBI GATnn'nnATC
Cuts at: 6175 6175
Size: 6181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4370 5005 275
Size: 53 83 186 936 2455 382 635 1451
Fragments arranged by size:
2455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4614 5012 5286 5433 5844 4614
Size: 398 274 147 411 4951
Fragments arranged by size:
4951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 2420 2450 2646
Size: 30 165 30 801 30 186 30 196
Cuts at: 2646 2676 1178
Size: 30 4683
Fragments arranged by size:
4683 801 196 186 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2933 3110 1186
Size: 1747 177 4257
Fragments arranged by size:
4257 1747 177
BseYI C'CCAG_C
Cuts at: 2101 2406 2786 2847 3012 3019 3032 3276 5384
Size: 305 380 61 165 7 13 244 2108
Cuts at: 5384 5880 2101
Size: 496 2402
Fragments arranged by size:
2402 2108 496 380 305 244 165 61
13 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2948 1163
Size: 573 393 819 4396
Fragments arranged by size:
4396 819 573 393
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3224 3631 4392 5420 1540
Size: 275 186 1223 407 761 1028 2301
Fragments arranged by size:
2301 1223 1028 761 407 275 186
BsiHKAI G_wGCw'C
Cuts at: 2861 3421 4242 4327 5398 2861
Size: 560 821 85 1071 3644
Fragments arranged by size:
3644 1071 821 560 85
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
BsmBI CGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 6181
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2404 2471 2741 2861 2915 3063
Size: 399 318 296 67 270 120 54 148
Cuts at: 3063 3231 3237 3303 3320 3421 3500 4242 4327
Size: 168 6 66 17 101 79 742 85
Cuts at: 4327 5398 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 318 296 270 168
148 120 101 85 79 67 66 54
17 6
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 4070 5800 4070
Size: 1730 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 2985 3417 3480 3536 844
Size: 1313 828 432 63 56 3489
Fragments arranged by size:
3489 1313 828 432 63 56
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2550 4676 4850 65
Size: 1183 1302 2126 174 1396
Fragments arranged by size:
2126 1396 1302 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2590 3219 4825 5012 1700
Size: 110 780 629 1606 187 2869
Fragments arranged by size:
2869 1606 780 629 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2638 794
Size: 1844 4337
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 2311 3511 3878 4263 4280 5732 5818 5830
Size: 339 1200 367 385 17 1452 86 12
Cuts at: 5830 6176 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1200 385 367 346 339 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2643 3294 2643
Size: 651 5530
BtgI C'CryG_G
Cuts at: 513 1065 2004 3151 3184 3393 513
Size: 552 939 1147 33 209 3301
Fragments arranged by size:
3301 1147 939 552 209 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5442
Fragments arranged by size:
5442 683 56
BtsI GCAGTG_nn'
Cuts at: 2327 2623 2984 3110 3697 4491 4511 2327
Size: 296 361 126 587 794 20 3997
Fragments arranged by size:
3997 794 587 361 296 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DraIII CAC_nnn'GTG
Cuts at: 2764 2764
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5188 809
Size: 507 3872 1802
Fragments arranged by size:
3872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2592 3221
Size: 54 1425 50 275 18 403 359 629
Cuts at: 3221 3396 3628 4517 6146 8
Size: 175 232 889 1629 43
Fragments arranged by size:
1629 1425 889 629 403 359 275 232
175 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3221 3628 1537
Size: 275 1409 407 4090
Fragments arranged by size:
4090 1409 407 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2340 2526 3076 4111 1353
Size: 869 118 186 550 1035 3423
Fragments arranged by size:
3423 1035 869 550 186 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3206 4768 5142 5288 387
Size: 1064 138 1617 1562 374 146 1280
Fragments arranged by size:
1617 1562 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3419 3419
Size: 6181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2422 2481 2923 4244 4841 5628
Size: 393 659 131 59 442 1321 597 787
Cuts at: 5628 5896 1239
Size: 268 1524
Fragments arranged by size:
1524 1321 787 659 597 442 393 268
131 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2642 2666 2306
Size: 336 24 5821
Fragments arranged by size:
5821 336 24
EcoO109I rG'GnC_Cy
Cuts at: 2467 2678 2692 2776 2827 3227 3299 3300 2467
Size: 211 14 84 51 400 72 1 5348
Fragments arranged by size:
5348 400 211 84 72 51 14 1
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 2197 5328 1678
Size: 519 3131 2531
Fragments arranged by size:
3131 2531 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 2734 2766 3032
Size: 32 135 32 980 32 150 32 266
Cuts at: 3032 3064 4822 4854 4896 4928 1373
Size: 32 1758 32 42 32 2626
Fragments arranged by size:
2626 1758 980 266 150 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2584 3525 3771 669
Size: 988 281 646 941 246 3079
Fragments arranged by size:
3079 988 941 646 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6181
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 3429 5295 5479 1305
Size: 930 1194 1866 184 2007
Fragments arranged by size:
2007 1866 1194 930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3398 10
Size: 54 1425 343 1566 2793
Fragments arranged by size:
2793 1566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2272 2336 2577 3260 4139 4498
Size: 648 991 115 64 241 683 879 359
Cuts at: 4498 4657 518
Size: 159 2042
Fragments arranged by size:
2042 991 879 683 648 359 241 159
115 64
MspA1I CmG'CkG
Cuts at: 1606 2022 2172 2381 3019 3186 3597 4274 5422
Size: 416 150 209 638 167 411 677 1148
Cuts at: 5422 5667 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 638 416 411 245 209
167 150
NaeI GCC'GGC
Cuts at: 3221 3221
Size: 6181
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4690
Fragments arranged by size:
4690 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NgoMIV G'CCGG_C
Cuts at: 3219 3219
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 3539 5084 1132
Size: 97 2310 1545 2229
Fragments arranged by size:
2310 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6181
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3019 1108
Size: 1911 4270
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2025 2057 4250 4282 5763 5795 2025
Size: 32 2193 32 1481 32 2411
Fragments arranged by size:
2411 2193 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2678 2692 2678
Size: 14 6167
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PspOMI G'GGCC_C
Cuts at: 2467 2737 3227 3299 2467
Size: 270 490 72 5349
Fragments arranged by size:
5349 490 270 72
PspXI vC'TCGA_Gb
Cuts at: 2397 3049 3313 2397
Size: 652 264 5265
Fragments arranged by size:
5265 652 264
PstI C_TGCA'G
Cuts at: 830 2021 2634 3117 3414 3533 830
Size: 1191 613 483 297 119 3478
Fragments arranged by size:
3478 1191 613 483 297 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2022 3019 2022
Size: 997 5184
SacI G_AGCT'C
Cuts at: 3421 3421
Size: 6181
SacII CC_GC'GG
Cuts at: 3187 3187
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SanDI GG'GwC_CC
Cuts at: 2692 2692
Size: 6181
SapI GCTCTTCn'nnn_
Cuts at: 2340 2340
Size: 6181
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SfcI C'TryA_G
Cuts at: 826 1045 2017 2630 3113 3410 3529 4664 5345
Size: 219 972 613 483 297 119 1135 681
Cuts at: 5345 5536 826
Size: 191 1471
Fragments arranged by size:
1471 1135 972 681 613 483 297 219
191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 2282 2675 3134 3508 1872
Size: 410 393 459 374 4545
Fragments arranged by size:
4545 459 410 393 374
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2397 3049 3313 4285 5186 5448
Size: 197 926 454 652 264 972 901 262
Cuts at: 5448 820
Size: 1553
Fragments arranged by size:
1553 972 926 901 652 454 264 262
197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 2262 4105 5
Size: 47 2210 1843 2081
Fragments arranged by size:
2210 2081 1843 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6181
StyI C'CwwG_G
Cuts at: 513 1065 2004 3194 3239 3620 513
Size: 552 939 1190 45 381 3074
Fragments arranged by size:
3074 1190 939 552 381 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2428 2555 3356 4208 4225 4378 4563
Size: 1255 848 127 801 852 17 153 185
Cuts at: 4563 5008 325
Size: 445 1498
Fragments arranged by size:
1498 1255 852 848 801 445 185 153
127 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4427 6139 96
Size: 274 80 33 51 1443 2450 1712 138
Fragments arranged by size:
2450 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2646 2678 6141 6173 2646
Size: 32 3463 32 2654
Fragments arranged by size:
3463 2654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 2397 3049 3313 1943
Size: 454 652 264 4811
Fragments arranged by size:
4811 652 454 264
XmaI C'CCGG_G
Cuts at: 1870 2280 2673 3132 3506 1870
Size: 410 393 459 374 4545
Fragments arranged by size:
4545 459 410 393 374
XmnI GAAnn'nnTTC
Cuts at: 4310 4310
Size: 6181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PsrI RsrII SexAI
SfiI SfoI SrfI SwaI XcmI