Restriction map for
pFN21AE1457
(Circular) (Six-base) MAPSORT of: pFN21AE1457.seq Check: 4187 from: 1 to: 8512
pFN21AE1457 8512 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2538 2625 3006 3325 3769 4480 2538
Size: 87 381 319 444 711 6570
Fragments arranged by size:
6570 711 444 381 319 87
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6322 278
Size: 53 83 186 936 4786 2468
Fragments arranged by size:
4786 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5616 5855 1937
Size: 3679 239 4594
Fragments arranged by size:
4594 3679 239
Acc65I G'GTAC_C
Cuts at: 5833 5833
Size: 8512
AclI AA'CG_TT
Cuts at: 1563 6639 7012 1563
Size: 5076 373 3063
Fragments arranged by size:
5076 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2297 2906 3362 4179 4993 5687 6575
Size: 506 159 609 456 817 814 694 888
Cuts at: 6575 7959 8227 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 888 817 814 694 609 506
456 268 159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8315
AflIII A'CryG_T
Cuts at: 1203 4353 5495 5619 7411 8388 1203
Size: 3150 1142 124 1792 977 1327
Fragments arranged by size:
3150 1792 1327 1142 977 124
AgeI A'CCGG_T
Cuts at: 7343 7343
Size: 8512
AhdI GACnn_n'nnGTC
Cuts at: 4345 7241 4345
Size: 2896 5616
AleI CACnn'nnGTG
Cuts at: 1166 2018 1166
Size: 852 7660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8512
AlwNI CAG_nnn'CTG
Cuts at: 2176 2611 3340 3442 4309 4423 4824 5336 5540
Size: 435 729 102 867 114 401 512 204
Cuts at: 5540 6255 7827 2176
Size: 715 1572 2861
Fragments arranged by size:
2861 1572 867 729 715 512 435 401
204 114 102
ApaI G_GGCC'C
Cuts at: 3264 3409 3991 3264
Size: 145 582 7785
Fragments arranged by size:
7785 582 145
ApaLI G'TGCA_C
Cuts at: 2525 2810 6569 7725 2525
Size: 285 3759 1156 3312
Fragments arranged by size:
3759 3312 1156 285
ApoI r'AATT_y
Cuts at: 1493 2281 2902 5821 6047 6199 8308 1493
Size: 788 621 2919 226 152 2109 1697
Fragments arranged by size:
2919 2109 1697 788 621 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7066 160
Size: 872 6034 1606
Fragments arranged by size:
6034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8512
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 2262 2720 3402 4472 4573 5837
Size: 73 207 112 458 682 1070 101 1264
Cuts at: 5837 1870
Size: 4545
Fragments arranged by size:
4545 1264 1070 682 458 207 112 101
73
BamHI G'GATC_C
Cuts at: 5842 6209 5842
Size: 367 8145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2716 3018 3215 4478 4828
Size: 325 217 627 929 302 197 1263 350
Cuts at: 4828 5153 5833 7288 618
Size: 325 680 1455 1842
Fragments arranged by size:
1842 1455 1263 929 680 627 350 325
325 302 217 197
BanII G_rGCy'C
Cuts at: 1391 3264 3409 3991 4101 4134 4574 5831 1391
Size: 1873 145 582 110 33 440 1257 4072
Fragments arranged by size:
4072 1873 1257 582 440 145 110 33
BbeI G_GCGC'C
Cuts at: 5157 5157
Size: 8512
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3849 4167 4716 4801 4869 5364 928
Size: 971 1950 318 549 85 68 495 4076
Fragments arranged by size:
4076 1950 971 549 495 318 85 68
BbvCI CC'TCA_GC
Cuts at: 2048 2405 2477 2762 2860 3029 4911 2048
Size: 357 72 285 98 169 1882 5649
Fragments arranged by size:
5649 1882 357 285 169 98 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6703 6737 1903
Size: 34 4766 34 3678
Fragments arranged by size:
4766 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6404 7614 6404
Size: 1210 7302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2258 4476
Size: 107 122 71 631 605 337 249 2218
Cuts at: 4476 5351 5725 7123 136
Size: 875 374 1398 1525
Fragments arranged by size:
2218 1525 1398 875 631 605 374 337
249 122 107 71
BglII A'GATC_T
Cuts at: 3636 8507 3636
Size: 4871 3641
BlpI GC'TnA_GC
Cuts at: 2876 5660 5929 2876
Size: 2784 269 5459
Fragments arranged by size:
5459 2784 269
Bme1580I G_kGCm'C
Cuts at: 1790 2363 2529 2721 2814 3023 3264 3409 3912
Size: 573 166 192 93 209 241 145 503
Cuts at: 3912 3991 4481 5455 6573 7729 1790
Size: 79 490 974 1118 1156 2573
Fragments arranged by size:
2573 1156 1118 974 573 503 490 241
209 192 166 145 93 79
BmgBI CAC'GTC
Cuts at: 1318 2686 3487 4354 5498 1318
Size: 1368 801 867 1144 4332
Fragments arranged by size:
4332 1368 1144 867 801
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3640 4130 4834
Size: 416 53 83 399 24 2223 490 704
Cuts at: 4834 6243 7201 442
Size: 1409 958 1753
Fragments arranged by size:
2223 1753 1409 958 704 490 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3212 1056
Size: 2156 6356
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5566 5566
Size: 8512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 2513 2681 3011 3464 4238 4760 5072
Size: 1154 120 168 330 453 774 522 312
Cuts at: 5072 5218 5806 7172 1239
Size: 146 588 1366 2579
Fragments arranged by size:
2579 1366 1154 774 588 522 453 330
312 168 146 120
Bpu10I CC'TnA_GC
Cuts at: 2048 2405 2477 2762 2860 3029 4911 2048
Size: 357 72 285 98 169 1882 5649
Fragments arranged by size:
5649 1882 357 285 169 98 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2356 3581 3899 4391 5252 6637 7502 7800 2356
Size: 1225 318 492 861 1385 865 298 3068
Fragments arranged by size:
3068 1385 1225 865 861 492 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4583 7175 882
Size: 617 3084 2592 2219
Fragments arranged by size:
3084 2592 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2815 3976 4153 6303 493
Size: 711 1611 1161 177 2150 2702
Fragments arranged by size:
2702 2150 1611 1161 711 177
BsaBI GATnn'nnATC
Cuts at: 8506 8506
Size: 8512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4602 5154 6319 6701
Size: 53 83 186 936 3069 552 1165 382
Cuts at: 6701 7336 275
Size: 635 1451
Fragments arranged by size:
3069 1451 1165 936 635 552 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2285 5505 6945 7343 7617 7764 8175 2285
Size: 3220 1440 398 274 147 411 2622
Fragments arranged by size:
3220 2622 1440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3605 3635 3782 3812 4229
Size: 30 165 30 2202 30 147 30 417
Cuts at: 4229 4259 5216 5246 1178
Size: 30 957 30 4444
Fragments arranged by size:
4444 2202 957 417 165 147 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 3401 3761 3782 4712 4787 4859 4862
Size: 982 1233 360 21 930 75 72 3
Cuts at: 4862 4886 4934 4949 5231 5432 1186
Size: 24 48 15 282 201 4266
Fragments arranged by size:
4266 1233 982 930 360 282 201 75
72 48 24 21 15 3
BseYI C'CCAG_C
Cuts at: 2587 2628 2741 3410 4411 5165 7715 8211 2587
Size: 41 113 669 1001 754 2550 496 2888
Fragments arranged by size:
2888 2550 1001 754 669 496 113 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2597 4106 4421 5188 1163
Size: 573 861 1509 315 767 4487
Fragments arranged by size:
4487 1509 861 767 573 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5720 5962 6723 7751 1540
Size: 275 186 2371 1348 242 761 1028 2301
Fragments arranged by size:
2371 2301 1348 1028 761 275 242 186
BsiHKAI G_wGCw'C
Cuts at: 2529 2547 2627 2814 3597 4101 5513 6573 6658
Size: 18 80 187 783 504 1412 1060 85
Cuts at: 6658 7729 2529
Size: 1071 3312
Fragments arranged by size:
3312 1412 1071 1060 783 504 187 85
80 18
BsmI GAATG_Cn'
Cuts at: 4296 6023 6116 4296
Size: 1727 93 6692
Fragments arranged by size:
6692 1727 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5504 5504
Size: 8512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2363 2529 2547 2627 2721 2814 3023
Size: 399 573 166 18 80 94 93 209
Cuts at: 3023 3264 3409 3597 3912 3991 4101 4134 4481
Size: 241 145 188 315 79 110 33 347
Cuts at: 4481 4574 5455 5513 5831 6573 6658 7729 1391
Size: 93 881 58 318 742 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 573 399 347 318
315 241 209 188 166 145 110 94
93 93 85 80 79 58 33 18
BspEI T'CCGG_A
Cuts at: 2285 8175 2285
Size: 5890 2622
BspHI T'CATG_A
Cuts at: 6401 8131 6401
Size: 1730 6782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 2538 2625 2655 3006 3325 3769 4480
Size: 1310 384 87 30 351 319 444 711
Cuts at: 4480 4809 5389 5867 844
Size: 329 580 478 3489
Fragments arranged by size:
3489 1310 711 580 478 444 384 351
329 319 87 30
BsrBI CCG'CTC
Cuts at: 1394 1823 4054 5716 6399 1394
Size: 429 2231 1662 683 3507
Fragments arranged by size:
3507 2231 1662 683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2707 4555 5755 7007 7181 65
Size: 1183 1459 1848 1200 1252 174 1396
Fragments arranged by size:
1848 1459 1396 1252 1200 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4440 5724 7156 7343 1700
Size: 110 2630 1284 1432 187 2869
Fragments arranged by size:
2869 2630 1432 1284 187 110
BsrGI T'GTAC_A
Cuts at: 96 2730 4188 4329 96
Size: 2634 1458 141 4279
Fragments arranged by size:
4279 2634 1458 141
BssHII G'CGCG_C
Cuts at: 1925 2248 4393 1925
Size: 323 2145 6044
Fragments arranged by size:
6044 2145 323
BssSI C'ACGA_G
Cuts at: 2540 6572 7584 2540
Size: 4032 1012 3468
Fragments arranged by size:
4032 3468 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2611 3158 4309 5211 794
Size: 1817 547 1151 902 4095
Fragments arranged by size:
4095 1817 1151 902 547
BstBI TT'CG_AA
Cuts at: 7316 7316
Size: 8512
BstEII G'GTnAC_C
Cuts at: 1184 3061 1184
Size: 1877 6635
BstXI CCAn_nnnn'nTGG
Cuts at: 2659 2914 4110 4672 2659
Size: 255 1196 562 6499
Fragments arranged by size:
6499 1196 562 255
BstYI r'GATC_y
Cuts at: 1972 2689 3636 5178 5842 6209 6594 6611 8063
Size: 717 947 1542 664 367 385 17 1452
Cuts at: 8063 8149 8161 8507 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1542 1452 947 717 664 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2935 2935
Size: 8512
BtgI C'CryG_G
Cuts at: 513 1065 2004 2363 3231 3559 4248 4455 4652
Size: 552 939 359 868 328 689 207 197
Cuts at: 4652 513
Size: 4373
Fragments arranged by size:
4373 939 868 689 552 359 328 207
197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2226 2606 3771 487
Size: 683 56 1000 380 1165 5228
Fragments arranged by size:
5228 1165 1000 683 380 56
BtsI GCAGTG_nn'
Cuts at: 2852 4076 4635 5541 6028 6822 6842 2852
Size: 1224 559 906 487 794 20 4522
Fragments arranged by size:
4522 1224 906 794 559 487 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8477
DraI TTT'AAA
Cuts at: 5816 5816
Size: 8512
DraIII CAC_nnn'GTG
Cuts at: 3061 4124 5211 3061
Size: 1063 1087 6362
Fragments arranged by size:
6362 1087 1063
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4805 7519 809
Size: 507 3489 2714 1802
Fragments arranged by size:
3489 2714 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 4169 4369
Size: 54 1425 50 275 18 330 2009 200
Cuts at: 4369 4442 4674 4780 5605 5717 5808 5959 6848
Size: 73 232 106 825 112 91 151 889
Cuts at: 6848 8477 8
Size: 1629 43
Fragments arranged by size:
2009 1629 1425 889 825 330 275 232
200 151 112 106 91 73 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 5717 5959 1537
Size: 275 2557 1348 242 4090
Fragments arranged by size:
4090 2557 1348 275 242
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 2958 4764 5042 5507 6442 1353
Size: 1094 511 1806 278 465 935 3423
Fragments arranged by size:
3423 1806 1094 935 511 465 278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4697 7099 7473 7619 387
Size: 1064 138 3108 2402 374 146 1280
Fragments arranged by size:
3108 2402 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4099 4099
Size: 8512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2297 2393 2513 2681 2906 3011
Size: 393 506 159 96 120 168 225 105
Cuts at: 3011 3362 3464 4179 4238 4760 4993 5072 5218
Size: 351 102 715 59 522 233 79 146
Cuts at: 5218 5687 5806 6575 7172 7959 8227 1239
Size: 469 119 769 597 787 268 1524
Fragments arranged by size:
1524 787 769 715 597 522 506 469
393 351 268 233 225 168 159 146
120 119 105 102 96 79 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2150
Size: 8512
EcoO109I rG'GnC_Cy
Cuts at: 3103 3268 3406 3439 3921 3987 5631 5803 3103
Size: 165 138 33 482 66 1644 172 5812
Fragments arranged by size:
5812 1644 482 172 165 138 66 33
EcoRI G'AATT_C
Cuts at: 2281 2902 5821 6199 2281
Size: 621 2919 378 4594
Fragments arranged by size:
4594 2919 621 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 5293 5545 2935
Size: 2358 252 5902
Fragments arranged by size:
5902 2358 252
FspI TGC'GCA
Cuts at: 3710 7018 3710
Size: 3308 5204
HaeII r_GCGC'y
Cuts at: 1678 5157 5199 5793 7659 1678
Size: 3479 42 594 1866 2531
Fragments arranged by size:
3479 2531 1866 594 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 3495 3527 3605
Size: 32 135 32 734 32 1157 32 78
Cuts at: 3605 3637 4331 4363 4561 4593 7153 7185 7227
Size: 32 694 32 198 32 2560 32 42
Cuts at: 7227 7259 1373
Size: 32 2626
Fragments arranged by size:
2626 2560 1157 734 694 198 135 78
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3703 4530 5856 6102 669
Size: 988 281 1765 827 1326 246 3079
Fragments arranged by size:
3079 1765 1326 988 827 281 246
HindIII A'AGCT_T
Cuts at: 748 3678 748
Size: 2930 5582
HpaI GTT'AAC
Cuts at: 6102 6102
Size: 8512
KasI G'GCGC_C
Cuts at: 5153 5153
Size: 8512
KpnI G_GTAC'C
Cuts at: 5837 5837
Size: 8512
MfeI C'AATT_G
Cuts at: 6111 6111
Size: 8512
MluI A'CGCG_T
Cuts at: 8388 8388
Size: 8512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2931 3879 4374 5538 5577 7626 7810 1305
Size: 1626 948 495 1164 39 2049 184 2007
Fragments arranged by size:
2049 2007 1626 1164 948 495 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4782 5607 10
Size: 54 1425 343 2950 825 2915
Fragments arranged by size:
2950 2915 1425 825 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2393 3863 5624 6470 6829 6988
Size: 648 852 375 1470 1761 846 359 159
Cuts at: 6988 518
Size: 2042
Fragments arranged by size:
2042 1761 1470 852 846 648 375 359
159
MspA1I CmG'CkG
Cuts at: 1606 2052 2350 2365 3164 3202 3242 3248 3414
Size: 446 298 15 799 38 40 6 166
Cuts at: 3414 3496 3618 4654 4690 4910 5772 5928 6605
Size: 82 122 1036 36 220 862 156 677
Cuts at: 6605 7753 7998 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1036 862 799 677 446 298
245 220 166 156 122 82 40 38
36 15 6
NaeI GCC'GGC
Cuts at: 5726 5726
Size: 8512
NarI GG'CG_CC
Cuts at: 5154 5154
Size: 8512
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7021
Fragments arranged by size:
7021 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8512
NgoMIV G'CCGG_C
Cuts at: 5724 5724
Size: 8512
NheI G'CTAG_C
Cuts at: 1052 3208 1052
Size: 2156 6356
NotI GC'GGCC_GC
Cuts at: 5717 5959 5717
Size: 242 8270
NspI r_CATG'y
Cuts at: 1132 1229 2392 2570 3352 5623 5698 5870 7415
Size: 97 1163 178 782 2271 75 172 1545
Cuts at: 7415 1132
Size: 2229
Fragments arranged by size:
2271 2229 1545 1163 782 178 172 97
75
PasI CC'CwG_GG
Cuts at: 1348 5042 1348
Size: 3694 4818
PciI A'CATG_T
Cuts at: 5619 7411 5619
Size: 1792 6720
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3442 1108
Size: 2334 6178
PfoI T'CCnGG_A
Cuts at: 2328 3105 3741 4583 2328
Size: 777 636 842 6257
Fragments arranged by size:
6257 842 777 636
PmeI GTTT'AAAC
Cuts at: 5816 5816
Size: 8512
PmlI CAC'GTG
Cuts at: 2815 4153 2815
Size: 1338 7174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6581 6613 8094 8126 6581
Size: 32 1481 32 6967
Fragments arranged by size:
6967 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3103 3268 3921 5631 3103
Size: 165 653 1710 5984
Fragments arranged by size:
5984 1710 653 165
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8512
PsiI TTA'TAA
Cuts at: 6082 6082
Size: 8512
PspOMI G'GGCC_C
Cuts at: 3260 3405 3987 3260
Size: 145 582 7785
Fragments arranged by size:
7785 582 145
PspXI vC'TCGA_Gb
Cuts at: 4472 4472
Size: 8512
PstI C_TGCA'G
Cuts at: 830 2172 2535 2607 3017 3195 3241 3247 3253
Size: 1342 363 72 410 178 46 6 6
Cuts at: 3253 3780 4305 4569 4820 5864 830
Size: 527 525 264 251 1044 3478
Fragments arranged by size:
3478 1342 1044 527 525 410 363 264
251 178 72 46 6 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8512
PvuII CAG'CTG
Cuts at: 2350 3202 3242 3248 3496 4690 2350
Size: 852 40 6 248 1194 6172
Fragments arranged by size:
6172 1194 852 248 40 6
SacI G_AGCT'C
Cuts at: 4101 4101
Size: 8512
SacII CC_GC'GG
Cuts at: 2366 4655 2366
Size: 2289 6223
SalI G'TCGA_C
Cuts at: 1936 5854 1936
Size: 3918 4594
SanDI GG'GwC_CC
Cuts at: 5631 5631
Size: 8512
SapI GCTCTTCn'nnn_
Cuts at: 2447 2447
Size: 8512
SbfI CC_TGCA'GG
Cuts at: 3780 5864 3780
Size: 2084 6428
ScaI AGT'ACT
Cuts at: 6760 8472 6760
Size: 1712 6800
SexAI A'CCwGG_T
Cuts at: 3057 3129 3057
Size: 72 8440
SfcI C'TryA_G
Cuts at: 826 1045 2168 2531 2603 3013 3191 3237 3243
Size: 219 1123 363 72 410 178 46 6
Cuts at: 3243 3249 3776 4301 4565 4816 5389 5860 6995
Size: 6 527 525 264 251 573 471 1135
Cuts at: 6995 7676 7867 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1123 681 573 527 525 471
410 363 264 251 219 191 178 72
46 6 6
SfoI GGC'GCC
Cuts at: 5155 5155
Size: 8512
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8512
SmaI CCC'GGG
Cuts at: 1872 3404 4575 5839 1872
Size: 1532 1171 1264 4545
Fragments arranged by size:
4545 1532 1264 1171
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2335 3596 3914 4406 4472 5267
Size: 197 926 392 1261 318 492 66 795
Cuts at: 5267 6616 7517 7779 820
Size: 1349 901 262 1553
Fragments arranged by size:
1553 1349 1261 926 901 795 492 392
318 262 197 66
SnaBI TAC'GTA
Cuts at: 493 3976 6303 493
Size: 3483 2327 2702
Fragments arranged by size:
3483 2702 2327
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8512
SphI G_CATG'C
Cuts at: 1132 2570 5870 1132
Size: 1438 3300 3774
Fragments arranged by size:
3774 3300 1438
SrfI GCCC'GGGC
Cuts at: 3404 3404
Size: 8512
SspI AAT'ATT
Cuts at: 5 52 6436 5
Size: 47 6384 2081
Fragments arranged by size:
6384 2081 47
StuI AGG'CCT
Cuts at: 1517 5672 1517
Size: 4155 4357
StyI C'CwwG_G
Cuts at: 513 1065 2004 3264 3935 4014 4269 5703 5951
Size: 552 939 1260 671 79 255 1434 248
Cuts at: 5951 513
Size: 3074
Fragments arranged by size:
3074 1434 1260 939 671 552 255 248
79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2642 2651 2797 3710 3972 4112 4816
Size: 1255 1062 9 146 913 262 140 704
Cuts at: 4816 5305 5428 5614 6539 6556 6709 6894 7339
Size: 489 123 186 925 17 153 185 445
Cuts at: 7339 325
Size: 1498
Fragments arranged by size:
1498 1255 1062 925 913 704 489 445
262 186 185 153 146 140 123 17
9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 4188 4329
Size: 274 80 33 51 1443 753 1458 141
Cuts at: 4329 4956 5481 6758 8470 96
Size: 627 525 1277 1712 138
Fragments arranged by size:
1712 1458 1443 1277 753 627 525 274
141 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3856 3888 4931 4963 5487 5519 5684 5716 8472
Size: 32 1043 32 524 32 165 32 2756
Cuts at: 8472 8504 3856
Size: 32 3864
Fragments arranged by size:
3864 2756 1043 524 165 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8512
XbaI T'CTAG_A
Cuts at: 5848 5848
Size: 8512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2986 3271 2389
Size: 597 285 7630
Fragments arranged by size:
7630 597 285
XhoI C'TCGA_G
Cuts at: 1943 4472 1943
Size: 2529 5983
XmaI C'CCGG_G
Cuts at: 1870 3402 4573 5837 1870
Size: 1532 1171 1264 4545
Fragments arranged by size:
4545 1532 1264 1171
XmnI GAAnn'nnTTC
Cuts at: 5432 6641 5432
Size: 1209 7303
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6320 276
Size: 53 83 186 936 4786 2468
Fragments arranged by size:
4786 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII
SfiI SwaI